Difference between revisions of "Biosafety.html"

(Created page with "<!DOCTYPE html> <html> <head> <title>DEI AGRA Wiki Page</title> <meta name="description" content="website description" /> <meta name="keywords" content="website keywor...")
 
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</head>
 
</head>
  
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<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
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          <li class="show"><img width="900" height="250" src="images/home_1.jpg" /></li>
+
none;text-autospace:none'><b><span lang=EN-US style='font-size:12.0pt;
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          <li><img width="900" height="250" src="images/home_2.jpg" /></li>
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line-height:150%;font-family:"Times New Roman","serif"'>&quot;Small Mistakes in
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Biosafety could have terrible global consequences&quot;<o:p></o:p></span></b></p>
−
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+
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  <div id="content">
+
−
          <div class="content_item">
+
−
  <div><h2 id='htd'> <b>"Small Mistakes in Biosafety could have terrible global consequences"</b></h2></div>
+
−
  <div><h2 id='htd'> <b>By-Thomas Ingle sky</b></h2></div>
+
−
          <div><h1><strong>(Centre of biosecurity at UNIVERSITY OF PITTSBURGH)</strong></h1></div>
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</div>
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<div class="content_imagetext1">
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<p>Biosafety refers to individual safeguards designed to protect laboratory personnel, as well as the surrounding environment and community. The biosafety protocols are divided into “BIOLOGICAL SAFETY LEVELS (BSL), which are series of protection relegated to autoclave related activities that take place in particular labs.</p>
+
−
    <p>These levels are generally standardized across the world, however their implementation varies from country to country, labs to labs, organization to organizations.</p>
+
−
    <p>These biosafety levels are ranked from 1 to 4, on the basis of agents or organisms that are being researched or worked on, in any given laboratory settings and are determined by the following-Risks related to containment, Severity of infection, Transmissibility, Nature of the work conducted, Origin of the microbe, Agent in question, Route of exposure</p>
+
−
            <h3><u><strong>BIOSAFETY IN INDIA</strong></u></h3>
+
−
            <p>IN India, a three tier mechanism comprising Institutional Biosafety Committees (IBSC) at the Institute/ company; the Review Committee on Genetic Manipulation (RCGM) in the Department of Biotechnology; and the Genetic Engineering Approval Committee (GEAC) in the Ministry of Environment & Forests (MoE&F), do analysis for granting approval for research and development activities on recombinant DNA products, environmental release of genetically engineered (GE)crops and monitoring and evaluation of research activities involving recombinant DNA technology has been established.</p>
+
−
    <p>In India, GMOs and products thereof are regulated as per the “Rules for the manufacture, use/import/export and storage of hazardous microorganisms/ genetically engineered organisms or cells, 1989” (commonly referred as Rules, 1989) notified by the Ministry of Environment and Forests (MoEF), Government of India under the Environment (Protection) Act (1986). These rules are implemented by MoEF, the Department of Biotechnology (DBT), Ministry of Science and Technology and the State Governments through the six competent authorities notified under the Rules which are as follows:</p>
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    <ul>
+
−
<li>Recombinant DNA Advisory Committee (RDAC)</li>
+
−
            <li>Institutional Biosafety Committee (IBSC)</li>
+
−
              <li> Review Committee on Genetic Manipulation (RCGM)</li>
+
−
                <li>Genetic Engineering Appraisal Committee (GEAC)</li>
+
−
                <li>State Biotechnology Coordination Committee (SBCC)</li>
+
−
                <li>District Level Committee (DLC).</li>
+
−
            </ul>
+
−
          <p>While the RDAC has advisory in function, IBSC, RCGM, and GEAC</p>
+
−
  </div>
+
−
+
−
  </div><!--close content_imagetext-->
+
−
 
+
−
 
+
−
  <div class="content_item"><!-------start TEAM INSTRUCTORS-->
+
−
          <div><h1><strong>FOLLOWING STEPS ARE INVOLVED IN THE ANALYSIS OF A GENETICALLY MODIFIED CELL -</strong></h1></div>
+
−
 
+
−
 
+
−
  <div class="content_imagetext1">
+
−
    <div class="content_image1">
+
−
      <img width="199" height="284"src="images/risk.png" alt="Risk"/>
+
−
    </div>
+
−
 
+
−
  <div>
+
−
    <h3 id='htd'> <b>"BIOSAFETY AT DAYALBAGH EDUCATIONAL INSTITUTE"</b></h3>
+
−
<p>We at Dayalbagh Educational Institute understand the importance of Biosafety and strongly believe in its enforcement, therefore our lab works and experiments are carried out under strict biosafety precautions.</p>
+
−
            <p>Before commencing our wet lab experiments we had received a training sessions of two days on safety and precautions to be maintained while handling the Instruments, glassware’s and reagents in a laboratory. </p>
+
−
<p>For the iGEM project we are working with the E.coli strain (DH5 a and BL-21 ), which falls under BSL-1(Biosafety level 1). Therefore all BSL-1 are implemented and followed during our laboratory workings</p>
+
−
    <p><b>STERILIZATION:</b> Clean, Dirt free environment is essential for getting good results. We used sanitizers and 70% ethanol for cleaning the platform. </p>
+
−
            <p><b>TOXICITY RISK:</b> Ethidium Bromide is the most commonly used staining dye for DNA, is highly toxic as mutagens. It may potentially cause carcinogenic effects. So for our team project we had decided to use a less toxic dye “GEL RED” purchased from GCC biotech. </p>
+
−
            <p><b>HANDLING OF EQUIPMENT: </b> We used all necessary equipment’s such as Autoclave, U-V trans-illuminator, Biosafety Cabinet under the guidance of well-trained Lab-Personnel’s.</p>
+
  
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><b><i><span lang=EN-US style='font-size:12.0pt;
 +
line-height:150%;font-family:"Times New Roman","serif"'>by-<u>Thomas Inglesky</u><o:p></o:p></span></i></b></p>
  
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><b><u><span lang=EN-US style='font-size:12.0pt;
 +
line-height:150%;font-family:"Times New Roman","serif"'><span
 +
style='mso-spacerun:yes'>        </span>(centre of biosecurity at UNIVERSITY OF
 +
PITTSBURGH)<o:p></o:p></span></u></b></p>
  
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span lang=EN-US style='font-size:12.0pt;line-height:
 +
150%;font-family:"Times New Roman","serif"'><o:p>&nbsp;</o:p></span></p>
  
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span lang=EN-US style='font-size:12.0pt;line-height:
 +
150%;font-family:"Times New Roman","serif"'>Biosafety refers to individual
 +
safeguards designed to protect laboratory personnel, as well as the surrounding
 +
environment and community.The biosafety protocols are divided
 +
into&quot;BIOLOGICAL SAFETY LEVELS(BSL), which are series of protection
 +
relegated to autoclave related activities that takeplace in particular labs.<o:p></o:p></span></p>
  
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span lang=EN-US style='font-size:12.0pt;line-height:
 +
150%;font-family:"Times New Roman","serif"'>These levels are generally standardized
 +
across the world, however their implementation varies from country to country,
 +
labs to labs, organization to organizations.<o:p></o:p></span></p>
  
−
</div>
+
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
−
</div><!--close content_imagetext-->
+
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
−
+
none;text-autospace:none'><span lang=EN-US style='font-size:12.0pt;line-height:
−
<div class="content_imagetext1">
+
150%;font-family:"Times New Roman","serif"'>These biosafety levels are ranked
−
   
+
from 1 to 4, on the basis of agents or organisms that are being researched or
−
 
+
worked on, in any given laboratory settings and are determined by the
−
  <div>
+
following-Risks related to containment, Severity of infection, Transmissibility,
−
    <h2 id='htd'> <b>"WASTE MANAGEMENT FOR OUR WORK"</b></h2>
+
Nature of the work conducted, Origin of the microbe, Agent in question,Route of
−
<p><b>Different trash containers are available in the lab:</b>One for biological waste (this waste will be autoclaved before being thrown out). Infection materials are also decontaminated prior to disposal, generally through the use of an autoclave.</p>
+
exposure<o:p></o:p></span></p>
−
            <p>One for common waste, special container for chemicals, Special container for waste broth and cultures </p>
+
−
</div>
+
−
</div><!--close content_imagetext-->
+
−
+
−
+
−
</div><!-------close TEAM INSTRUCTORS-->
+
−
 
+
−
  <div>
+
−
    <h2 id='htd'> <b>BASIC RULES FOLLOWED IN OUR LABORATORY</b></h2>
+
−
<p>In the Biosafety level 1 laboratory, personnel is allowed to work with low risk microbes that pose little or no threat of infection in healthy adults. Under BSL-1 following practices are permitted-</p>
+
−
    <ul>
+
−
<li>Mechanical pipetting only (no mouth pipetting allowed)</li>
+
−
            <li>Safe sharps handling</li>
+
−
              <li>Avoidance of splashes or aerosols</li>
+
−
                <li>Daily decontamination of all work surfaces when work is complete</li>
+
−
                <li>Hand washing</li>
+
−
                <li>Prohibition of food, drink and smoking materials in lab setting</li>
+
−
<li>Personal protective equipment, such as; eye protection, gloves and a lab coat or gown</li>
+
−
<li>Biohazard signs</li>
+
−
            </ul>
+
−
<p>Apart from following the basic guidelines, our team takes security and safety measures at the highest priority. All members had to wear clean Lab Coat during the practical sessions. Team members had to wear lab sleepers, glove, goggles and mask according to demand of work. We also ensure proper disposal of gloves, used pipette tips, Eppendorf tubes in a separate bio hazardous container.</p>
+
−
<p>IGEM 2017 -TEAM DEI ensures all the protocols and safety measures are followed and implemented during team's lab work.</p>
+
−
</div>
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
  
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><b style='mso-bidi-font-weight:normal'><u><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif"'>BIOSAFETY
 +
IN INDIA-<o:p></o:p></span></u></b></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span lang=EN-US style='font-size:12.0pt;line-height:
 +
150%;font-family:"Times New Roman","serif"'>IN India, a three tier mechanism
 +
comprising <b>InstitutionalBiosafetyCommittees</b> (<b>IBSC</b>) at the
 +
Institute/ company; the <b>ReviewCommitteeonGeneticManipulation</b> (<b>RCGM</b>)
 +
in the Department of Biotechnology; and the <b>GeneticEngineeringApprovalCommittee</b>
 +
(<b>GEAC</b>) in the Ministry of Environment &amp; Forests (MoE&amp;F), do
 +
analysis for granting approval for research and development activities on
 +
recombinant DNA products, environmental release of genetically engineered
 +
(GE)crops and monitoring and evaluation of research activities involving
 +
recombinant DNA technology has been established.<o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span lang=EN-US style='font-size:12.0pt;line-height:
 +
150%;font-family:"Times New Roman","serif"'><span
 +
style='mso-spacerun:yes'> </span>In India, GMOs and products thereof are
 +
regulated as per the “Rules for themanufacture, use/import/export and storage
 +
of hazardous microorganisms/ geneticallyengineered organisms or cells, 1989”
 +
(commonly referred as Rules, 1989) notified bythe Ministry of Environment and
 +
Forests (MoEF), Government of India under theEnvironment (Protection) Act
 +
(1986). These rules are implemented by MoEF, theDepartment of Biotechnology
 +
(DBT), Ministry of Science and Technology and theState Governments through the
 +
six competent authorities notified under the Ruleswhich are as follows:<o:p></o:p></span></p>
 +
 +
<p class=MsoListParagraphCxSpFirst style='margin-top:0cm;margin-right:0cm;
 +
margin-bottom:10.0pt;margin-left:54.0pt;mso-add-space:auto;text-align:justify;
 +
text-justify:inter-ideograph;text-indent:-36.0pt;line-height:150%;mso-pagination:
 +
none;mso-list:l3 level1 lfo3;mso-layout-grid-align:none;text-autospace:none'><![if !supportLists]><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif";
 +
mso-fareast-font-family:"Times New Roman"'><span style='mso-list:Ignore'>i.<span
 +
style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span lang=EN-US style='font-size:12.0pt;
 +
line-height:150%;font-family:"Times New Roman","serif"'>Recombinant DNA
 +
Advisory Committee (RDAC)<o:p></o:p></span></p>
 +
 +
<p class=MsoListParagraphCxSpMiddle style='margin-top:0cm;margin-right:0cm;
 +
margin-bottom:10.0pt;margin-left:54.0pt;mso-add-space:auto;text-align:justify;
 +
text-justify:inter-ideograph;text-indent:-36.0pt;line-height:150%;mso-pagination:
 +
none;mso-list:l3 level1 lfo3;mso-layout-grid-align:none;text-autospace:none'><![if !supportLists]><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif";
 +
mso-fareast-font-family:"Times New Roman"'><span style='mso-list:Ignore'>ii.<span
 +
style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span lang=EN-US style='font-size:12.0pt;
 +
line-height:150%;font-family:"Times New Roman","serif"'>Institutional Biosafety
 +
Committee (IBSC)<o:p></o:p></span></p>
 +
 +
<p class=MsoListParagraphCxSpMiddle style='margin-top:0cm;margin-right:0cm;
 +
margin-bottom:10.0pt;margin-left:54.0pt;mso-add-space:auto;text-align:justify;
 +
text-justify:inter-ideograph;text-indent:-36.0pt;line-height:150%;mso-pagination:
 +
none;mso-list:l3 level1 lfo3;mso-layout-grid-align:none;text-autospace:none'><![if !supportLists]><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif";
 +
mso-fareast-font-family:"Times New Roman"'><span style='mso-list:Ignore'>iii.<span
 +
style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span lang=EN-US style='font-size:12.0pt;
 +
line-height:150%;font-family:"Times New Roman","serif"'>Review Committee on
 +
Genetic Manipulation (RCGM)<o:p></o:p></span></p>
 +
 +
<p class=MsoListParagraphCxSpMiddle style='margin-top:0cm;margin-right:0cm;
 +
margin-bottom:10.0pt;margin-left:54.0pt;mso-add-space:auto;text-align:justify;
 +
text-justify:inter-ideograph;text-indent:-36.0pt;line-height:150%;mso-pagination:
 +
none;mso-list:l3 level1 lfo3;mso-layout-grid-align:none;text-autospace:none'><![if !supportLists]><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif";
 +
mso-fareast-font-family:"Times New Roman"'><span style='mso-list:Ignore'>iv.<span
 +
style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span lang=EN-US style='font-size:12.0pt;
 +
line-height:150%;font-family:"Times New Roman","serif"'>Genetic Engineering
 +
Appraisal Committee (GEAC)<o:p></o:p></span></p>
 +
 +
<p class=MsoListParagraphCxSpMiddle style='margin-top:0cm;margin-right:0cm;
 +
margin-bottom:10.0pt;margin-left:54.0pt;mso-add-space:auto;text-align:justify;
 +
text-justify:inter-ideograph;text-indent:-36.0pt;line-height:150%;mso-pagination:
 +
none;mso-list:l3 level1 lfo3;mso-layout-grid-align:none;text-autospace:none'><![if !supportLists]><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif";
 +
mso-fareast-font-family:"Times New Roman"'><span style='mso-list:Ignore'>v.<span
 +
style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span lang=EN-US style='font-size:12.0pt;
 +
line-height:150%;font-family:"Times New Roman","serif"'>State Biotechnology
 +
Coordination Committee (SBCC)<o:p></o:p></span></p>
 +
 +
<p class=MsoListParagraphCxSpLast style='margin-top:0cm;margin-right:0cm;
 +
margin-bottom:10.0pt;margin-left:54.0pt;mso-add-space:auto;text-align:justify;
 +
text-justify:inter-ideograph;text-indent:-36.0pt;line-height:150%;mso-pagination:
 +
none;mso-list:l3 level1 lfo3;mso-layout-grid-align:none;text-autospace:none'><![if !supportLists]><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif";
 +
mso-fareast-font-family:"Times New Roman"'><span style='mso-list:Ignore'>vi.<span
 +
style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span lang=EN-US style='font-size:12.0pt;
 +
line-height:150%;font-family:"Times New Roman","serif"'>District Level
 +
Committee (DLC).<o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span lang=EN-US style='font-size:12.0pt;line-height:
 +
150%;font-family:"Times New Roman","serif"'>While the RDAC has advisory in
 +
function, IBSC, RCGM, and GEAC<o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><b><u><span lang=EN-US style='font-size:12.0pt;
 +
line-height:150%;font-family:"Times New Roman","serif";text-transform:uppercase'>Following
 +
steps are involved in the analysis of a Genetically Modified Cell -<o:p></o:p></span></u></b></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman","serif";mso-ansi-language:EN-IN;mso-fareast-language:
 +
EN-IN;mso-bidi-language:AR-SA;mso-no-proof:yes'><v:shapetype id="_x0000_t75"
 +
coordsize="21600,21600" o:spt="75" o:preferrelative="t" path="m@4@5l@4@11@9@11@9@5xe"
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filled="f" stroked="f">
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<v:stroke joinstyle="miter"/>
 +
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 +
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 +
<v:imagedata src="https://static.igem.org/mediawiki/2017/thumb/f/f3/Biosafety_01.jpg/800px-Biosafety_01.jpg" o:title="Slide1"
 +
  croptop="7522f" cropbottom="2961f" cropright="3601f"/>
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 +
font-family:"Times New Roman","serif"'><o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
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inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
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none;text-autospace:none'><b style='mso-bidi-font-weight:normal'><u><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif"'><o:p><span
 +
style='text-decoration:none'>&nbsp;</span></o:p></span></u></b></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
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none;text-autospace:none'><b style='mso-bidi-font-weight:normal'><u><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif"'><o:p><span
 +
style='text-decoration:none'>&nbsp;</span></o:p></span></u></b></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><a name="_GoBack"></a><b style='mso-bidi-font-weight:
 +
normal'><u><span lang=EN-US style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman","serif"'><o:p><span style='text-decoration:none'>&nbsp;</span></o:p></span></u></b></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><b style='mso-bidi-font-weight:normal'><u><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif"'>BIOSAFETY
 +
AT DAYALBAGH EDUCATIONAL INSTITUTE-<o:p></o:p></span></u></b></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span lang=EN-US style='font-size:12.0pt;line-height:
 +
150%;font-family:"Times New Roman","serif"'>We at Dayalbagh Educational
 +
Institute understand the importance of Biosafety and strongly believe in its
 +
enforcement, therefore our lab works and experiments are carried out under
 +
strict biosafety precautions.<o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span lang=EN-US style='font-size:12.0pt;line-height:
 +
150%;font-family:"Times New Roman","serif"'>Before commencing our wet lab
 +
experiments we had received a training sessions of two days on safety and
 +
precautions to be maintained while handling the Instruments, glassware’s
 +
and<span style='mso-spacerun:yes'>  </span>reagents in a laboratory. <o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span lang=EN-US style='font-size:12.0pt;line-height:
 +
150%;font-family:"Times New Roman","serif"'>For the iGEM project we are working
 +
with the <i style='mso-bidi-font-style:normal'><u>E</u>.<u>coli</u></i>strain (DH5
 +
&#945; and BL-21), which falls under BSL-1(Biosafety level 1). Therefore all
 +
BSL-1 are implemented and followed during our laboratory workings<o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><b style='mso-bidi-font-weight:normal'><u><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif";
 +
text-transform:uppercase'>Sterilization:</span></u></b><span lang=EN-US
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif"'>
 +
Clean, Dirt free environment is essential for getting good results. We used
 +
sanitizers and 70% ethanol for cleaning the platform. <o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><b style='mso-bidi-font-weight:normal'><u><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif";
 +
text-transform:uppercase'>Toxicity Risk:</span></u></b><span lang=EN-US
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif"'>
 +
Ethidium Bromide is the most commonly used staining dye for DNA, is highly
 +
toxic as mutagens. It may potentially cause carcinogenic effects. So for our
 +
team project we had decided to use a less toxic dye “GEL RED” purchased from
 +
GCC biotech.<o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><b style='mso-bidi-font-weight:normal'><u><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif";
 +
text-transform:uppercase'>Handling of equipment:</span></u></b><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif"'>
 +
We used all necessary equipment’s such as Autoclave, U-V trans-illuminator, Biosafety
 +
Cabinet under the guidance of well-trained Lab-Personnel’s.<o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><b><u><span lang=EN-US style='font-size:12.0pt;
 +
line-height:150%;font-family:"Times New Roman","serif"'>WASTE MANAGEMENT FOR
 +
OUR WORK-<o:p></o:p></span></u></b></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span lang=EN-US style='font-size:12.0pt;line-height:
 +
150%;font-family:"Times New Roman","serif"'>Different trash containers are
 +
available in the lab:<o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span lang=EN-US style='font-size:12.0pt;line-height:
 +
150%;font-family:"Times New Roman","serif"'>One for biological waste (this
 +
waste will be autoclaved before being thrown out). Infection materials are also
 +
decontaminated prior to disposal, generally through the use of an autoclave.<o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span lang=EN-US style='font-size:12.0pt;line-height:
 +
150%;font-family:"Times New Roman","serif"'>One for common waste,special
 +
container for chemicals, Special container for waste broth and cultures<o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><b style='mso-bidi-font-weight:normal'><u><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif"'>BASIC<span
 +
style='mso-spacerun:yes'>  </span>RULES<span style='mso-spacerun:yes'> 
 +
</span>FOLLOWED IN OUR LABORATORY: <o:p></o:p></span></u></b></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span lang=EN-US style='font-size:12.0pt;line-height:
 +
150%;font-family:"Times New Roman","serif"'>In the Biosafety level 1
 +
laboratory, personnel is allowed to work with low risk microbes that pose
 +
little or no threat of infection in healthy adults. Under BSL-1 following
 +
practices are permitted-<o:p></o:p></span></p>
 +
 +
<p class=MsoListParagraph style='margin-bottom:10.0pt;mso-add-space:auto;
 +
text-align:justify;text-justify:inter-ideograph;text-indent:-18.0pt;line-height:
 +
150%;mso-pagination:none;mso-list:l2 level1 lfo6;mso-layout-grid-align:none;
 +
text-autospace:none'><![if !supportLists]><span lang=EN-US style='font-size:
 +
12.0pt;line-height:150%;font-family:Symbol;mso-fareast-font-family:Symbol;
 +
mso-bidi-font-family:Symbol'><span style='mso-list:Ignore'>·<span
 +
style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span lang=EN-US style='font-size:12.0pt;
 +
line-height:150%;font-family:"Times New Roman","serif"'>Mechanical pipetting
 +
only (no mouth pipetting allowed)<o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-top:0cm;margin-right:0cm;margin-bottom:10.0pt;
 +
margin-left:36.0pt;text-align:justify;text-justify:inter-ideograph;text-indent:
 +
-18.0pt;line-height:150%;mso-pagination:none;mso-list:l0 level1 lfo1;
 +
mso-layout-grid-align:none;text-autospace:none'><![if !supportLists]><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:Symbol;
 +
mso-fareast-font-family:Symbol;mso-bidi-font-family:Symbol'><span
 +
style='mso-list:Ignore'>·<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span lang=EN-US style='font-size:12.0pt;
 +
line-height:150%;font-family:"Times New Roman","serif"'>Safe sharps handling<o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-top:0cm;margin-right:0cm;margin-bottom:10.0pt;
 +
margin-left:36.0pt;text-align:justify;text-justify:inter-ideograph;text-indent:
 +
-18.0pt;line-height:150%;mso-pagination:none;mso-list:l0 level1 lfo1;
 +
mso-layout-grid-align:none;text-autospace:none'><![if !supportLists]><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:Symbol;
 +
mso-fareast-font-family:Symbol;mso-bidi-font-family:Symbol'><span
 +
style='mso-list:Ignore'>·<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span lang=EN-US style='font-size:12.0pt;
 +
line-height:150%;font-family:"Times New Roman","serif"'>Avoidance of splashes
 +
or aerosols<o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-top:0cm;margin-right:0cm;margin-bottom:10.0pt;
 +
margin-left:36.0pt;text-align:justify;text-justify:inter-ideograph;text-indent:
 +
-18.0pt;line-height:150%;mso-pagination:none;mso-list:l0 level1 lfo1;
 +
mso-layout-grid-align:none;text-autospace:none'><![if !supportLists]><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:Symbol;
 +
mso-fareast-font-family:Symbol;mso-bidi-font-family:Symbol'><span
 +
style='mso-list:Ignore'>·<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span lang=EN-US style='font-size:12.0pt;
 +
line-height:150%;font-family:"Times New Roman","serif"'>Daily decontamination
 +
of all work surfaces when work is complete<o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-top:0cm;margin-right:0cm;margin-bottom:10.0pt;
 +
margin-left:36.0pt;text-align:justify;text-justify:inter-ideograph;text-indent:
 +
-18.0pt;line-height:150%;mso-pagination:none;mso-list:l0 level1 lfo1;
 +
mso-layout-grid-align:none;text-autospace:none'><![if !supportLists]><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:Symbol;
 +
mso-fareast-font-family:Symbol;mso-bidi-font-family:Symbol'><span
 +
style='mso-list:Ignore'>·<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span lang=EN-US style='font-size:12.0pt;
 +
line-height:150%;font-family:"Times New Roman","serif"'>Hand washing<o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-top:0cm;margin-right:0cm;margin-bottom:10.0pt;
 +
margin-left:36.0pt;text-align:justify;text-justify:inter-ideograph;text-indent:
 +
-18.0pt;line-height:150%;mso-pagination:none;mso-list:l0 level1 lfo1;
 +
mso-layout-grid-align:none;text-autospace:none'><![if !supportLists]><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:Symbol;
 +
mso-fareast-font-family:Symbol;mso-bidi-font-family:Symbol'><span
 +
style='mso-list:Ignore'>·<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span lang=EN-US style='font-size:12.0pt;
 +
line-height:150%;font-family:"Times New Roman","serif"'>Prohibition of food,
 +
drink and smoking materials in lab setting<o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-top:0cm;margin-right:0cm;margin-bottom:10.0pt;
 +
margin-left:36.0pt;text-align:justify;text-justify:inter-ideograph;text-indent:
 +
-18.0pt;line-height:150%;mso-pagination:none;mso-list:l0 level1 lfo1;
 +
mso-layout-grid-align:none;text-autospace:none'><![if !supportLists]><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:Symbol;
 +
mso-fareast-font-family:Symbol;mso-bidi-font-family:Symbol'><span
 +
style='mso-list:Ignore'>·<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span lang=EN-US style='font-size:12.0pt;
 +
line-height:150%;font-family:"Times New Roman","serif"'>Personal protective
 +
equipment, such as; eye protection, gloves and a lab coat or gown<o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-top:0cm;margin-right:0cm;margin-bottom:10.0pt;
 +
margin-left:36.0pt;text-align:justify;text-justify:inter-ideograph;text-indent:
 +
-18.0pt;line-height:150%;mso-pagination:none;mso-list:l0 level1 lfo1;
 +
mso-layout-grid-align:none;text-autospace:none'><![if !supportLists]><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:Symbol;
 +
mso-fareast-font-family:Symbol;mso-bidi-font-family:Symbol'><span
 +
style='mso-list:Ignore'>·<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span lang=EN-US style='font-size:12.0pt;
 +
line-height:150%;font-family:"Times New Roman","serif"'>Biohazard signs<o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span lang=EN-US style='font-size:12.0pt;line-height:
 +
150%;font-family:"Times New Roman","serif"'>Apart from following the basic
 +
guidelines, our team takes security and safety measures at the highest
 +
priority. All members had to wear clean Lab Coat during the practical sessions.
 +
Team members had to wear lab sleepers, glove, goggles and mask according to
 +
demand of work. We also ensure proper disposal of gloves, used pipette tips,
 +
Eppendorf tubes in a separate bio hazardous container.<o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><b style='mso-bidi-font-weight:normal'><span
 +
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif"'>IGEM
 +
2017 -TEAM DEI</span></b><span lang=EN-US style='font-size:12.0pt;line-height:
 +
150%;font-family:"Times New Roman","serif"'> ensures all the protocols and
 +
safety measures are followed and implemented during team's lab work.<o:p></o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span lang=EN-US style='font-size:12.0pt;line-height:
 +
150%;font-family:"Times New Roman","serif"'><o:p>&nbsp;</o:p></span></p>
 +
 +
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Revision as of 09:58, 1 November 2017

"Small Mistakes in Biosafety could have terrible global consequences"

by-Thomas Inglesky

        (centre of biosecurity at UNIVERSITY OF PITTSBURGH)

 

Biosafety refers to individual safeguards designed to protect laboratory personnel, as well as the surrounding environment and community.The biosafety protocols are divided into"BIOLOGICAL SAFETY LEVELS(BSL), which are series of protection relegated to autoclave related activities that takeplace in particular labs.

These levels are generally standardized across the world, however their implementation varies from country to country, labs to labs, organization to organizations.

These biosafety levels are ranked from 1 to 4, on the basis of agents or organisms that are being researched or worked on, in any given laboratory settings and are determined by the following-Risks related to containment, Severity of infection, Transmissibility, Nature of the work conducted, Origin of the microbe, Agent in question,Route of exposure

BIOSAFETY IN INDIA-

IN India, a three tier mechanism comprising InstitutionalBiosafetyCommittees (IBSC) at the Institute/ company; the ReviewCommitteeonGeneticManipulation (RCGM) in the Department of Biotechnology; and the GeneticEngineeringApprovalCommittee (GEAC) in the Ministry of Environment & Forests (MoE&F), do analysis for granting approval for research and development activities on recombinant DNA products, environmental release of genetically engineered (GE)crops and monitoring and evaluation of research activities involving recombinant DNA technology has been established.

 In India, GMOs and products thereof are regulated as per the “Rules for themanufacture, use/import/export and storage of hazardous microorganisms/ geneticallyengineered organisms or cells, 1989” (commonly referred as Rules, 1989) notified bythe Ministry of Environment and Forests (MoEF), Government of India under theEnvironment (Protection) Act (1986). These rules are implemented by MoEF, theDepartment of Biotechnology (DBT), Ministry of Science and Technology and theState Governments through the six competent authorities notified under the Ruleswhich are as follows:

i.                    Recombinant DNA Advisory Committee (RDAC)

ii.                  Institutional Biosafety Committee (IBSC)

iii.                Review Committee on Genetic Manipulation (RCGM)

iv.                Genetic Engineering Appraisal Committee (GEAC)

v.                  State Biotechnology Coordination Committee (SBCC)

vi.                District Level Committee (DLC).

While the RDAC has advisory in function, IBSC, RCGM, and GEAC

Following steps are involved in the analysis of a Genetically Modified Cell -

 

 

 

BIOSAFETY AT DAYALBAGH EDUCATIONAL INSTITUTE-

We at Dayalbagh Educational Institute understand the importance of Biosafety and strongly believe in its enforcement, therefore our lab works and experiments are carried out under strict biosafety precautions.

Before commencing our wet lab experiments we had received a training sessions of two days on safety and precautions to be maintained while handling the Instruments, glassware’s and  reagents in a laboratory.

For the iGEM project we are working with the E.colistrain (DH5 α and BL-21), which falls under BSL-1(Biosafety level 1). Therefore all BSL-1 are implemented and followed during our laboratory workings

Sterilization: Clean, Dirt free environment is essential for getting good results. We used sanitizers and 70% ethanol for cleaning the platform.

Toxicity Risk: Ethidium Bromide is the most commonly used staining dye for DNA, is highly toxic as mutagens. It may potentially cause carcinogenic effects. So for our team project we had decided to use a less toxic dye “GEL RED” purchased from GCC biotech.

Handling of equipment: We used all necessary equipment’s such as Autoclave, U-V trans-illuminator, Biosafety Cabinet under the guidance of well-trained Lab-Personnel’s.

WASTE MANAGEMENT FOR OUR WORK-

Different trash containers are available in the lab:

One for biological waste (this waste will be autoclaved before being thrown out). Infection materials are also decontaminated prior to disposal, generally through the use of an autoclave.

One for common waste,special container for chemicals, Special container for waste broth and cultures

BASIC  RULES  FOLLOWED IN OUR LABORATORY:

In the Biosafety level 1 laboratory, personnel is allowed to work with low risk microbes that pose little or no threat of infection in healthy adults. Under BSL-1 following practices are permitted-

·            Mechanical pipetting only (no mouth pipetting allowed)

·         Safe sharps handling

·         Avoidance of splashes or aerosols

·         Daily decontamination of all work surfaces when work is complete

·         Hand washing

·         Prohibition of food, drink and smoking materials in lab setting

·         Personal protective equipment, such as; eye protection, gloves and a lab coat or gown

·         Biohazard signs

Apart from following the basic guidelines, our team takes security and safety measures at the highest priority. All members had to wear clean Lab Coat during the practical sessions. Team members had to wear lab sleepers, glove, goggles and mask according to demand of work. We also ensure proper disposal of gloves, used pipette tips, Eppendorf tubes in a separate bio hazardous container.

IGEM 2017 -TEAM DEI ensures all the protocols and safety measures are followed and implemented during team's lab work.