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| | <img class="homeplaybutton" src="https://static.igem.org/mediawiki/2017/7/73/T--NKU_China--playbutton1.png"/> | | <img class="homeplaybutton" src="https://static.igem.org/mediawiki/2017/7/73/T--NKU_China--playbutton1.png"/> |
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| − | <div style="width:100%; padding-left:100px; padding-right:100px;" class="introduction"> | + | <div><img src="https://static.igem.org/mediawiki/2017/5/58/T--NKU_China--home1.png"/></div> |
| − | <div class="header">INTRODUCTION</div>
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| − | <div class="line"><img src="https://static.igem.org/mediawiki/2017/ b/ b8/T--NKU_China-- line.png"/></div> | + | |
| − | <div class="sub-header">DESCRIPTION</div> | + | |
| − | In the process of oil recovering by water injection method, it will form large water channels in the bottom. <br/><br/>
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| − | Water mainly flows through these channels, which stops brushing the oil hidden in the minor channel branches in soil, so that the remaining oil will be trapped into the branches. </br>
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| − | Our project is aiming to engineer Enterobacter sp. <i>FY-07</i> (which is separated from oilfield produced water and able to produce cellulose naturally), so as to controllably produce rhamnolipid and cellulose.</br>
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| − | Rhamnolipid is a biosurfactant with the ability of emulsifying oil, so the oil can be easily washed out.
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| − | Cellulose can clog large water channels, helping water enter into the gaps of soil and wash out the oil within them.</br><br/>
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| − | Our project is aimed at improving oil recovery rate in the oil harvesting process using this kind of bacteria.
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| − | <div class="sub-header">PROCESS ACHIEVED</div>
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| − | 1. We have cloned rhamnolipid synthesis gene (<i>rhIABC</i>) in Pseudomonas aeruginosa, and transformed it into <i>Escherichia coli BL21</i> and successfully achieved the expression of <i>rhIABC</i> in <i>E.coli BL21</i>.
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| − | 2. We have transformed rhIABC into <i>FY-07</i> and successfully fulfilled the co-production of rhamnolipid and cellulose.
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| − | 3. Based on <i>E.coli</i> fimbriae control system (<i>E.coli</i> fimbriae (Fim) phase variation system), we have finished the switch design, to whose both ends GFP and RFP have been linked. This modified switch has been transformed into <i>BL21</i> and proved to be feasible.
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| − | <div class="sub-header">FUTURE PLAN</div>
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| − | 1. Finish the construction of plasmid.
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| − | <br/><br/>
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| − | 2. Introduce molecular switch and rhamnolipid synthase into <i>BscA</i> gene knock out <i>FY-07</i> strain.
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| − | 3. Achieve controllable expression of rhamnolipid and cellulose in <i>FY-07</i> strain.
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| − | <br/><br/>
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| − | 4. Introduce suicide system and ensure the controllability.
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| − | <div class="header">COMMUNICATION AND CORPORATIONS</div>
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| − | <div class="line"><img src="https://static.igem.org/mediawiki/2017/b/b8/T--NKU_China--line.png"/></div>
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| − | 1. We expect to communicate with the practitioners in oil industry so as to know more about applying petroleum microorganisms into oil production. Please contact us if you are a practitioner in oil industry who has some knowledge in this field.
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| − | 2. We are looking for a suitable suicide system so that the bacteria will not survive in the environment for a long time. Please contact us if you have some recommendations.
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| − | 3. Please do not hesitate to contact us if you are interested in our project. We are looking forward to communicating with you!
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| − | </div>
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| − | </body>
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