Difference between revisions of "Team:Lambert GA/Integrated Practices"

 
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<center> <h1 id="MainTitle"><b>Integrated Practices</b></h1><img src="https://static.igem.org/mediawiki/2017/b/bc/T--Lambert_GA--purpleline.png" style="width:18%; margin:auto;"> </center> <br>
 
<center> <h1 id="MainTitle"><b>Integrated Practices</b></h1><img src="https://static.igem.org/mediawiki/2017/b/bc/T--Lambert_GA--purpleline.png" style="width:18%; margin:auto;"> </center> <br>
 
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<h2 style="text-align:center">Using Precision Metabolic Engineering to Save Lives </h2>
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<h2 style="text-align:center"> Engineering Design Principles</h2>
 
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Micronutrient deficiencies plague impoverished and developing nations throughout the world. According to the World Health Organization (WHO), over 2 billion people - representing more than 30% of the world’s population - suffer from micronutrient deficiencies. However, diagnosis of micronutrient deficiency has proven to be extremely expensive and often time-consuming. To address this growing concern, medical professionals and synthetic biologists have created simple, inexpensive biosensors that serve as handy diagnostic tests to use in the field. Nevertheless, these versatile tools still suffer from errors that can yield inaccurate results and even lead to faulty diagnosis. With this in mind, the 2017 Lambert iGEM Team focused primarily on how to enhance existing biosensors and subsequently aid in the diagnosis of micronutrient deficiencies throughout the world.
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Lambert iGEM used engineering design principles to modify their project. In order to improve the Chrome-Q, the team consulted with Mr. Gibson, the CEO and founder of Gibson Ridge Software. After receiving feedback, the team applied new changes to the Chrome-Q in order to fix previous mistakes. This correlates with the Engineering Principles because they were able to change certain aspects of the device based on error analysis.
 
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The Styczynski Group at the Georgia Institute of Technology (GaTech) is working on developing a zinc micronutrient biosensor. After collaborating with the lab at GaTech, we gained a better understanding of some of the problems that biosensor development often faced. Oftentimes, biosensors do not consistently yield accurate responses to specific signals because of severe leakiness that may exist in the system or fluctuations in the affinities of biosensing promoters used in the system. Moreover, the overexpression of one pigment (or GFP) over another can cause misreadings. Such was the case with GaTech’s zinc biosensor - the overexpression of the pigment Violacein over the other pigments caused the system to be difficult to interpret.
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<h2 style="text-align:center"> Maker Faire</h2>
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The Lambert iGEM team attended and presented at Maker Faire in Atlanta, Georgia. The team introduced the project to people of all ages, specifically focusing on protein degradation and quantifying data using a new device they constructed called the Chrome-Q. After describing the overview of the project, they asked the public, “Why is biology important to you?”
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<b>Click <a href="https://2017.igem.org/Team:Lambert_GA/HP/Silver">here</a> to read more.</b>
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<font color="white"><i>Conference at Georgia Tech with Dr. Styczynski and Monica McNerney</i></font>
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Consequently, we studied protein degradation and devised a “Switch” that attempts to prevent this sort of overexpression and subsequently fine tune the overall accuracy of biosensors in general. We used SsrA tagged reporters in an inducible plasmid construct to characterize the relative strength of degradation as well as learn more about the protease ClpXP. To further characterize ClpXP, we used three different strains of <i>E. coli</i> cells (DH10, Keio Wild ClpX Knockout, Keio Wild ClpP Knockout) from the ECSC Yale Keio registry. The Keio strains are specific <i>E. coli</i> strains that express distinct gene knockouts; we worked with the ClpP knockout (JWO428-1), ClpX Knockout (JWO427-1) and the Keio Wild strains. However, due to time constraints, we were only able to grow and gain results for the ClpP strain.
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<h2 style="text-align:center">Call with Dr. Saad Bhamla</h2>
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Lambert iGEM discussed their progress with the PaperFuge in the project with Dr. Saad Bhamla, a new Georgia Tech professor who was a part of the Prakash Lab at Stanford University. Dr. Bhamla also assisted with some details so the team could improve their own device, the Chrome-Q, to be more fundamental. The progress with these low-cost appliances help contribute to underfunded labs.  
 
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<h2 style="text-align:center">Femme in STEM</h2>
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As a part of the Women in STEM club led by iGEM members, the team prepared a brunch called “Femme in STEM.” This brunch allowed both teachers and students at Lambert High School to delve more into fields in STEM without feeling restricted due to gender roles in society. Lambert iGEM also was able to discuss their project this year to promote iGEM and biotechnology at the high school.
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<font color="white"><i>Lauren Hong and Jack Kwon working in Georgia Tech lab over the summer to run through workflow with Monica McNerney</i></font>
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Given the physical and financial constraints of a high school laboratory, we didn’t have the luxury of using a fluorometer to quantify the relative strength of degradation in GFP molecules. Therefore, we used chromoproteins because they were visible to the naked eye. We exploited the chromoproteins’ stark visibility and constructed the ChromQ - a 3D printed “camera system” that feasibly quantifies the chromoproteins in a light-controlled chamber. After taking pictures of the chromoproteins inside the ChromQ light-chamber, we converted the pixels from the pictures into RGB values by using a commercial photo-editing software. In order to standardize chromoprotein expression, we used Roscolux filters to create a color chart that correlates with each degree of degradation in chromoproteins.
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<font color="white"><i>Panelists at Atlanta Science Festival Discussion</i></font>
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To deliver a more immediate impact outside the lab, we collaborated with numerous institutions to increase awareness of micronutrient deficiencies and synthetic biology in general. For example, we partnered with the Atlanta Science Festival to hold a discussion about the merits of GMOs. The discussion was lead by a team of professors from the University of Georgia, Emory University, Georgia Institute of Technology, and Georgia State University. Titled “GMOs or GMnos”, the discussion was held at GaTech and received over 100 participants. Moreover, we also hosted a workshop at the Building with Biology event at the Atlanta Maker Faire. We used this event as a venue to educate the public about DNA extraction and the recently discovered CRISPR-Cas9 gene editing technology. We even collaborated with Next Generation Focus, a non-profit organization based in Forsyth County, to educate underprivileged students in Forsyth County about synthetic biology and biotechnology.
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<font color="white"><i>Lauren Hong educating people about CRISPR/Cas9 at the Maker Faire</i></font>
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All in all, from devising an inducible plasmid construct in order to fine tune existing biosensors and further characterize a known protease system to collaborating with various universities and nonprofit organizations, the 2017 Lambert iGEM Team has delivered an impact both inside and outside the scientific community.
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Latest revision as of 10:53, 1 November 2017


Integrated Practices



Engineering Design Principles


Lambert iGEM used engineering design principles to modify their project. In order to improve the Chrome-Q, the team consulted with Mr. Gibson, the CEO and founder of Gibson Ridge Software. After receiving feedback, the team applied new changes to the Chrome-Q in order to fix previous mistakes. This correlates with the Engineering Principles because they were able to change certain aspects of the device based on error analysis.

Maker Faire


The Lambert iGEM team attended and presented at Maker Faire in Atlanta, Georgia. The team introduced the project to people of all ages, specifically focusing on protein degradation and quantifying data using a new device they constructed called the Chrome-Q. After describing the overview of the project, they asked the public, “Why is biology important to you?”
Click here to read more.


Call with Dr. Saad Bhamla


Lambert iGEM discussed their progress with the PaperFuge in the project with Dr. Saad Bhamla, a new Georgia Tech professor who was a part of the Prakash Lab at Stanford University. Dr. Bhamla also assisted with some details so the team could improve their own device, the Chrome-Q, to be more fundamental. The progress with these low-cost appliances help contribute to underfunded labs.

Femme in STEM


As a part of the Women in STEM club led by iGEM members, the team prepared a brunch called “Femme in STEM.” This brunch allowed both teachers and students at Lambert High School to delve more into fields in STEM without feeling restricted due to gender roles in society. Lambert iGEM also was able to discuss their project this year to promote iGEM and biotechnology at the high school.