Line 112: | Line 112: | ||
<p> | <p> | ||
<ul class="ulclass"> | <ul class="ulclass"> | ||
− | <li> | + | <li>• Diethart Mattanovich, Michael Sauer and Hans Marx: organization of the iGEM project |
</li><li>• Gabi Wilt: ordered Materials for our Team | </li><li>• Gabi Wilt: ordered Materials for our Team | ||
− | </li><li>• | + | </li><li>• Corinna Rebnegger: helped us doing flow cytometric experiments for characterization of our pGAL syn |
− | </li><li>• Tom Gaßler: | + | </li><li>• Tom Gaßler: advice on setting up our CRISPR/Cas9 mediated gene integration in <i> Saccharomyces cerevisiae </i> by supporting us with primers for assembling the specific sgRNAs. |
− | </li><li>• Martin Altvater: provided us different plasmids for <i> Saccharomyces cerevisiae </i> ready for our Golden Gate system | + | </li><li>• Martin Altvater: provided us with different plasmids for <i> Saccharomyces cerevisiae </i> ready for our Golden Gate system |
− | </li><li>• Jürgen Zanghelini: supported us with | + | </li><li>• Jürgen Zanghelini: supported us with modeling |
</li><li>• Florian Strobl: advice and lab material | </li><li>• Florian Strobl: advice and lab material | ||
− | </li><li>• Gerald Striedner and Esther Egger provided us the | + | </li><li>• Gerald Striedner and Esther Egger provided us with the pSIM 5 and pSIM 9 plasmids |
− | + | ||
</li><li>• Franziska Doleschal: organized our bank account and managed all our invoices | </li><li>• Franziska Doleschal: organized our bank account and managed all our invoices | ||
</li><li>• Anna Gindl: designed our Logo | </li><li>• Anna Gindl: designed our Logo | ||
</li><li>• Nikolaus Huber: helped us setting up the core code of our wiki page | </li><li>• Nikolaus Huber: helped us setting up the core code of our wiki page | ||
− | </li><li>• | + | </li><li>• Sauer group: Provided us BB1_01 – BB1_08 |
− | </li><li>• | + | </li><li>• Mattanovich/ Gasser group: provided us material, all empty BBs for the Golden GATE system and many protocols: |
<ul class="ulclass"><li>• Preparation of chemical competent <i>E. coli</i> | <ul class="ulclass"><li>• Preparation of chemical competent <i>E. coli</i> | ||
− | </li><li>• Transformation chemical competent <i>E. coli</i> | + | </li><li>• <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#PreparationColi">Preparation chemical competent <i>E. coli</i></a> |
− | </li><li>• Preparation of electro competent <i>S. cerevisiae</i> | + | </li><li>• <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#TransformationColi">Transformation chemical competent <i>E. coli</i></a> |
− | </li><li>• Transformation of electro competent <i>S. cerevisiae</i> | + | </li><li>• <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#PreparationYeast">Preparation of electro competent <i>S. cerevisiae</i></a> |
− | </li><li>• mRNA extraction</li></ul></li> | + | </li><li>• <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#TransformationYeast">Transformation of electro competent <i>S. cerevisiae</i></a> |
+ | </li><li>• <a href="https://2017.igem.org/Team:BOKU-Vienna/Protocol#mRNA">mRNA extraction</li></ul></li></a> | ||
<li>• Bernd Albrecht: helped us doing the RT screenings on the Flow cytometer, by setting up the right parameters. </li></ul> | <li>• Bernd Albrecht: helped us doing the RT screenings on the Flow cytometer, by setting up the right parameters. </li></ul> | ||
</p> | </p> | ||
− | <p> Furthermore we want to thank Stefanie Wiesauer, | + | <p> Furthermore we want to thank Matthias Steiger, Stefanie Wiesauer, |
Revision as of 11:11, 1 November 2017
Attributions
In the course of our project we were able to conduct experiments never done before. However, in order to do so a base of knowledge and resources is required. Fortunately we encountered many ambitious people who supported us. We want to thank them all for kindly helping us during our project.
- • Diethart Mattanovich, Michael Sauer and Hans Marx: organization of the iGEM project
- • Gabi Wilt: ordered Materials for our Team
- • Corinna Rebnegger: helped us doing flow cytometric experiments for characterization of our pGAL syn
- • Tom Gaßler: advice on setting up our CRISPR/Cas9 mediated gene integration in Saccharomyces cerevisiae by supporting us with primers for assembling the specific sgRNAs.
- • Martin Altvater: provided us with different plasmids for Saccharomyces cerevisiae ready for our Golden Gate system
- • Jürgen Zanghelini: supported us with modeling
- • Florian Strobl: advice and lab material
- • Gerald Striedner and Esther Egger provided us with the pSIM 5 and pSIM 9 plasmids
- • Franziska Doleschal: organized our bank account and managed all our invoices
- • Anna Gindl: designed our Logo
- • Nikolaus Huber: helped us setting up the core code of our wiki page
- • Sauer group: Provided us BB1_01 – BB1_08
- • Mattanovich/ Gasser group: provided us material, all empty BBs for the Golden GATE system and many protocols:
- • Preparation of chemical competent E. coli
- • Preparation chemical competent E. coli
- • Transformation chemical competent E. coli
- • Preparation of electro competent S. cerevisiae
- • Transformation of electro competent S. cerevisiae
- • mRNA extraction
- • Bernd Albrecht: helped us doing the RT screenings on the Flow cytometer, by setting up the right parameters.
Furthermore we want to thank Matthias Steiger, Stefanie Wiesauer,