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<h4>With higher concentrations of salicylate, the response was again very quick but with greater magnitude. Saturation of fluorescence signal could not be studied with higher concentrations as the fluorescence crossed the upper-limit of the instrument. Thus, we observe that the <i>mar</i> promoter responds within 20 minutes to the salicylate.</h4> | <h4>With higher concentrations of salicylate, the response was again very quick but with greater magnitude. Saturation of fluorescence signal could not be studied with higher concentrations as the fluorescence crossed the upper-limit of the instrument. Thus, we observe that the <i>mar</i> promoter responds within 20 minutes to the salicylate.</h4> | ||
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<center> | <center> | ||
<table><tr><td width="22%"> | <table><tr><td width="22%"> | ||
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</tr></table> | </tr></table> | ||
</center></section> | </center></section> | ||
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+ | <h3>Cloning of chromoprotein for selection and part submission for registry :</h3> | ||
+ | <h4>We select five different chromoproteins for studying their kinetics to evaluate their utility in our circuit.</h4> | ||
+ | <h4>We have observed that out of these five different chromoproteins, chromoprotein sequence no. 1-3 develop color very fast (24-30 hours). But chromoproteins sequence no. 4-5 develop color vey late. Hence can not be suitable for utilization in our circuit. | ||
+ | Therefore, we select chromoprotein amilCP Blue chromoprotein and fwYellow chromoprotein for utilization circuit. | ||
+ | Further all these chromoproteins are cloned with RBS and LacI promoter by 3A assembly and submitted in depository with accession no. (from BBa_K2320001- K2320005). | ||
+ | </h4> | ||
+ | <center><table><tr><td><img src="https://static.igem.org/mediawiki/2017/a/a4/T--IISER-Mohali-INDIA--dish.jpg" alt="Dishes"></td></tr></table></center> | ||
+ | <center><table><tr><td><img src="https://static.igem.org/mediawiki/2017/8/83/T--IISER-Mohali-INDIA--dish2.jpg" alt="Dishes"></td></tr></table></center> | ||
<section> | <section> |
Revision as of 11:41, 1 November 2017