In this year’s collaboration, NJU_china helped us to test the microRNA binding ability of miRNA binding sequences. They constructed a dual-luciferase miRNA target expression plasmid with their vector and our miRNA target sequences. Then they transfected the purified plasmids into cells and verify their functions.

In return, we performed nanoparticle tracking analysis (NTA) to have a more precise determination of the quantity and size of secreted exosomes. Extracellular vesicles, as nanoparticles, can be automatically tracked and sized based on Brownian motion and the diffusion coefficient. We measured the Nanosight enabled quantification and size determination of the extracellular vesicles in our experiment. The result we got from nanoparticle tracking analysis (NTA) can help NJU_china ascertain the relationship between protein concentration and exosomes quantity.

Figure 1 exosomes magnified 100000x

Figure 2 exosomes magnified 200000x

On this year’s FAFU CCiC (Fujian Agricultural and Forestry University Conference of China’s iGEMers Community), we were interested in the program from team BGIC-Union. And it was pleasure that we had chance to communicate with them. The split T7 report system which they brought up was really original and potential. We provided them plasmid sHRPN-dcas9 and sHRPC-dcas9 which could be used in their program’s detection. We designed these two plasmids in last year’s program. It was pleasure to hear that our design could provide convenience to them in their plasmid construction and signal transformation. We also showed them the plasmid profiles and design ideas of these plasmids. As return, they also gave us some useful advice in our studies. We really benefited a lot from the communication with BGIC-Union, not only in academic area, but also in friendship.