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<h3> PCR Masterlist Spreadsheet</h3></br> | <h3> PCR Masterlist Spreadsheet</h3></br> | ||
By the end of our project we had done a variety of different PCRs, with different primers, DNA templates, and conditions, therefore we made a spreadsheet in order to keep track of them all, so members of the team could generate their own spreadsheet and print it out if they were doing a large number of reactions at once. We recommend that other teams do this early on, as we could have saved a lot of time by implementing this system from the start of the project! </br> | By the end of our project we had done a variety of different PCRs, with different primers, DNA templates, and conditions, therefore we made a spreadsheet in order to keep track of them all, so members of the team could generate their own spreadsheet and print it out if they were doing a large number of reactions at once. We recommend that other teams do this early on, as we could have saved a lot of time by implementing this system from the start of the project! </br> | ||
− | <img | + | <img src="https://static.igem.org/mediawiki/2017/0/0e/T--Oxford--Results--pcrspreadsheet.png"> |
</br> | </br> | ||
<h3>Double Digest Masterlist</h3> | <h3>Double Digest Masterlist</h3> | ||
We also made our own version of the NEB double-digest poster, with which buffers we could use with which restriction enzymes, because although HF-versions of enzymes and Cutsmart has been around for a while, there are still lots of non-HF versions of the enzymes around in our lab. Our version was simplified and only included the enzymes we were using frequently, this saved a lot of time when running lots of digestions simultaneously, this is something else we recommend teams do early on. | We also made our own version of the NEB double-digest poster, with which buffers we could use with which restriction enzymes, because although HF-versions of enzymes and Cutsmart has been around for a while, there are still lots of non-HF versions of the enzymes around in our lab. Our version was simplified and only included the enzymes we were using frequently, this saved a lot of time when running lots of digestions simultaneously, this is something else we recommend teams do early on. | ||
</br> | </br> | ||
− | <img src="https://static.igem.org/mediawiki/2017/4/4d/T--Oxford--Results--digestmasterlist.png"> | + | <img width="200px" src="https://static.igem.org/mediawiki/2017/4/4d/T--Oxford--Results--digestmasterlist.png"> |
</br> | </br> | ||
<h3>Numbering System</h3></br> | <h3>Numbering System</h3></br> |
Revision as of 11:48, 1 November 2017
Shipping Vector Cloning
Introduction
We have included this page to summarise the techniques and results we had in cloning our parts into the pSB1C3. For a detailed look at the protocols themselves and every step of the process please visit our protocols page.