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<div class="box3 left biosafety" href="https://2017.igem.org/Team:TAS_Taipei/Safety"> | <div class="box3 left biosafety" href="https://2017.igem.org/Team:TAS_Taipei/Safety"> | ||
− | <h1> | + | <h1>Safety</h1> |
</div> | </div> | ||
<div class="box3 left about" href="https://2017.igem.org/Team:TAS_Taipei/Team"> | <div class="box3 left about" href="https://2017.igem.org/Team:TAS_Taipei/Team"> | ||
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<li> | <li> | ||
<a href="#CGU" class="pageNavBig">CGU_Taiwan</a> | <a href="#CGU" class="pageNavBig">CGU_Taiwan</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="#lambert" class="pageNavBig">LAMBERT_GA</a> | ||
</li> | </li> | ||
</ul> | </ul> | ||
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<div class="row"> | <div class="row"> | ||
<h4 class="para col-lg-8"> | <h4 class="para col-lg-8"> | ||
− | We have continued and strengthened our long standing partnership with National Yang Ming University (NYMU_Taipei). | + | We have continued and strengthened our long-standing partnership with National Yang Ming University (NYMU_Taipei). They troubleshooted our cloning procedure and design of primers, gave us protocols for assaying biofilm production, and trained our future iGEMers (Leona and Catherine). <br><br> |
In return, we helped add to the characterization of their lactose-induced suicide mechanism: the Holin-Endolysin-NrtA system (<a href="http://parts.igem.org/Part:BBa_K2350021">BBa_K2350021</a>). We measured the population of their kill-switch bacteria in different lactose concentrations, and find that our results are consistent with their findings. | In return, we helped add to the characterization of their lactose-induced suicide mechanism: the Holin-Endolysin-NrtA system (<a href="http://parts.igem.org/Part:BBa_K2350021">BBa_K2350021</a>). We measured the population of their kill-switch bacteria in different lactose concentrations, and find that our results are consistent with their findings. | ||
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</h4> | </h4> | ||
</div> | </div> | ||
− | </div> | + | </div><br> |
<div class="row"> | <div class="row"> | ||
<h4 class="para col-lg-12"> | <h4 class="para col-lg-12"> | ||
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<div class="row"> | <div class="row"> | ||
<h4 class="para col-lg-8"> | <h4 class="para col-lg-8"> | ||
− | We first met the CGU_Taiwan team at the end of our presentation for the Asia Pacific iGEM Conference hosted | + | We first met the CGU_Taiwan team at the end of our presentation for the Asia Pacific iGEM Conference hosted at National Chiao Tung University (NCTU). <br><br> |
− | They were excited that our biofilms were able to trap nanoparticles and | + | They were excited that our biofilms were able to trap nanoparticles, and wondered if they could trap ink particles as well. CGU_Taiwan is working on improving the procedure to manufacture reprocessed paper. They found that a process called “flotation,” typically used to de-ink paper, could cause significant paper fiber loss. They wondered if biofilm could potentially replace the current flotation process to preserve paper fibers. We offered to test this for CGU_Taiwan. We used a similar experimental procedure as our preliminary biofilm trapping experiment. The results suggest that <b>our biofilm can trap ink particles</b>. As shown below, the relative absorbance of ink decreased by approximately 50% when mixed with biofilm. |
</h4> | </h4> | ||
<div class="image_container col-lg-4"> | <div class="image_container col-lg-4"> | ||
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</h4> | </h4> | ||
</div> | </div> | ||
− | </div> | + | </div><br> |
+ | <div class="row"> | ||
+ | <div class="image_container col-lg-10 col-lg-offset-1"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/7/70/T--TAS_Taipei--CGU_DATA_.jpg.png" alt="test" id="group"> | ||
+ | <h4 class="subtitle">When ink was mixed with biofilm, we saw a 50% decrease in absorbance, compared to a sample containing only ink. <span class="subCred">Experiment: William Chen, Yvonne Wei</span> | ||
+ | </h4> | ||
+ | </div> | ||
+ | </div><br> | ||
<div class="row"> | <div class="row"> | ||
<h4 class="para col-lg-12"> | <h4 class="para col-lg-12"> | ||
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<div class="image_container col-lg-10 col-lg-offset-1"> | <div class="image_container col-lg-10 col-lg-offset-1"> | ||
<img src="https://static.igem.org/mediawiki/2017/7/70/T--TAS_Taipei--figure_3-18.jpg" alt="test" id="group"> | <img src="https://static.igem.org/mediawiki/2017/7/70/T--TAS_Taipei--figure_3-18.jpg" alt="test" id="group"> | ||
− | <h4 class="subtitle"> | + | <h4 class="subtitle"><b>A)</b> Our experimental results showed that <i>E. coli</i> overexpressing OmpR234 (BBa_K2229200) is producing more biofilm than a control which does not overexpress OmpR234 (BBa_K342003). <b>B)</b> CGU_Taiwan independently tested our constructs using crystal violet, a dye commonly used to quantify biofilm formation. BBa_K2229200 showed higher absorbance compared to the control BBa_K342003, reflecting the formation of more biofilm, which matches our results. |
<span class="subCred">Experiment: Yvonne Wei</span> | <span class="subCred">Experiment: Yvonne Wei</span> | ||
</h4> | </h4> | ||
</div> | </div> | ||
</div> | </div> | ||
+ | <div class="row" id="lambert"> | ||
+ | <h1 class="title2 col-lg-12">LAMBERT_GA</h1> | ||
+ | </div> | ||
+ | <div class="row"> | ||
+ | <h4 class="para col-lg-8"> | ||
+ | We received and 3D printed LAMBERT_GA’s Chrome-Q device. We used this device with our synthetic biology class when they started to clone DNA constructs that expressed GFP. Students monitored the effect of temperature on GFP degradation at 25, 37, 60 and 100 deg C. We also had a great video chat with their entire team in May 2017 to share project ideas & topics, summer plans, iGEM HS life, etc. We always enjoy seeing them at the jamboree!! | ||
+ | </h4> | ||
+ | <div class="image_container col-lg-4"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/6/68/T--TAS_Taipei--Lambert.jpg" alt="test" id="group"> | ||
+ | </div> | ||
+ | </div><br> | ||
+ | <div class="row"> | ||
+ | <div class="image_container col-lg-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/3/34/T--TAS_Taipei--Chrome-min.JPG" alt="test" id="group"> | ||
+ | </div> | ||
+ | <div class="image_container col-lg-6"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/8/8e/T--TAS_Taipei--LambertVideo-min.JPG" alt="test" id="group"> | ||
+ | </div> | ||
+ | </div><br> | ||
</section> | </section> | ||
</div> | </div> |
Latest revision as of 12:21, 1 November 2017
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Project
Experiments
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About Us
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Project
Experiment
Modeling
Prototype
Human Practice
Safety
About Us
Attributions
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