Difference between revisions of "Team:Kyoto/Achievements"

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　　We attributed all works done by outside collaborators accurately and thoroughly.<br>  
 
　　We attributed all works done by outside collaborators accurately and thoroughly.<br>  
 
Visit our attribution page.(<a href="https://2017.igem.org/Team:Kyoto/Attributions">
 
Visit our attribution page.(<a href="https://2017.igem.org/Team:Kyoto/Attributions">
−
https://2017.igem.org/Team:Kyoto/Attributions)
+
https://2017.igem.org/Team:Kyoto/Attributions</a>)
 
</li>
 
</li>
 
           <li>・Improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry.
 
           <li>・Improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry.

Revision as of 13:24, 1 November 2017

Achievements


Bronze
  • ・Expand on our silver medal activity by demonstrating how we have integrated the investigated issues into the design and/or execution of our project.
    We discuss the pine disease problems with forestry associations and conducted the forestry survey. We really gathered the nematodes in the forest and observed them in the lab. We fed S. cerevisiae with our reporter protein（BBa_K2403003, http://parts.igem.org/Part:BBa_K2403003）to them and investigated how many percent of the wild nematodes we can use for our project. As a result, we couldn’t confirm the nematodes except for B. xylophilus preyed S. cerevisiae. This suggested to us that our “B. x Buster” can specifically target B.xylophilus.
  • ・Improve the function of an existing BioBrick Part.
    　　We improved the following parts.
    ・BBa_J63006 (http://parts.igem.org/Part:BBa_J63006)
    ・BBa_K517001( ・BBa_K2403004(http://parts.igem.org/Part:BBa_K2403004 ) ・BBa_K2403005(http://parts.igem.org/Part:BBa_K2403005) We created a new part from BBa_J63006, BBa_K51701, and observed the strong fluorescence of EGFP in S. cerevisiae.() Furthermore, we succeeded in dying the digestive tract by GFP. We established the novel the usage and improved the function of the existing part.
    We confirmed BBa_K2403004 and BBa_K2403005 are useful for the expression of loop hairpin dsRNA in S. cerevisiae.
  • ・We confirmed that Our project works.
    We optimized how to culture and to take photograph, and finally succeeded in recording B.xylophilus preyed on S. cereviisae as a movie. We developed the fluorescent maker to distinguish the B. xylophilus and established the system which makes it possible to recognize only B. xylophilus which eats S. cerevisiae by a microscopy. We constructed the plasmid for S. cerevisiae to express dsRNA and determined the quantity.
    We confirmed we can observed the B. xylophilus selectively which preyed on S. cerevisiae expressing dsRNA by our fluorescent reporter. Visit our demonstrate page for details.( https://2017.igem.org/Team:Kyoto/Demonstrate”)
  • Integrated human practice Through this practice we tried to bridge the desire of the people who protect the pine, the damage caused by pine disease, and the theory (laboratory) and the practice (mountain).
    Integrated Human Practice
  • We attempted to convey the usefulness of GMO to people who showed a strong rejection response to genetically modified foods but failed. Based on this experience, it became a good opportunity to think about how we can listen to our opinions as to how to disagree with opinions.
    We had good opportunity to think about the way we get people who disagree with us listen to ours. Engagement