Difference between revisions of "Template:Heidelberg/best basic"
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This basic part exhibits a strong tendency to form silicon-carbon bonds and is, therefore, a valuable addition to perform controlled organic chemistry in microorganisms. | This basic part exhibits a strong tendency to form silicon-carbon bonds and is, therefore, a valuable addition to perform controlled organic chemistry in microorganisms. | ||
A triple mutant of this part has already been applied in the successful synthesis of organosilicons as a proof-of-concept. | A triple mutant of this part has already been applied in the successful synthesis of organosilicons as a proof-of-concept. | ||
| − | As a next step, this part can be implemented in the directed evolution approach of phage-assisted continuous evolution (PACE) or in the phage-related discontinuous evolution (PREDCEL) approach to improve organosilicon synthesis by cytochrome engineering.|https://static.igem.org/mediawiki/2017/ | + | As a next step, this part can be implemented in the directed evolution approach of phage-assisted continuous evolution (PACE) or in the phage-related discontinuous evolution (PREDCEL) approach to improve organosilicon synthesis by cytochrome engineering.|https://static.igem.org/mediawiki/2017/1/1d/T--Heidelberg--CytochromeCRMA.png}} |
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Revision as of 13:33, 1 November 2017
BBa_K2398000
We present as best basic part the codon-optimized version of the cytochrome c protein that is able to convert silicon educts to organosilicon products. This cytochrome c variant provides an easy to use tool that is accessible to everyone in the synthetic biology community and allows the user to harness the vast potential of organosilicons. This basic part exhibits a strong tendency to form silicon-carbon bonds and is, therefore, a valuable addition to perform controlled organic chemistry in microorganisms. A triple mutant of this part has already been applied in the successful synthesis of organosilicons as a proof-of-concept. As a next step, this part can be implemented in the directed evolution approach of phage-assisted continuous evolution (PACE) or in the phage-related discontinuous evolution (PREDCEL) approach to improve organosilicon synthesis by cytochrome engineering.
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