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This SOE2 PCR frament was tried to clone in low copy number plasmid pACYC177, at XhoI and XmaI site (high copy number plasmid). | This SOE2 PCR frament was tried to clone in low copy number plasmid pACYC177, at XhoI and XmaI site (high copy number plasmid). | ||
</h4> | </h4> | ||
+ | <h4>The E. coli transformants were screened for positive clones of construct 2 by restriction | ||
+ | digestion and obtained positive clone in lane 2,3,4,6,7,8,9,10 and 11 are shown in agraose gel | ||
+ | below.</h4> | ||
+ | |||
+ | <center><table><tr><td><img src="https://static.igem.org/mediawiki/2017/7/72/T--IISER-Mohali-INDIA--rslstss.png" alt="Flow chart"></td></tr></table></center> | ||
<center><table><tr><td><img src="https://static.igem.org/mediawiki/2017/3/31/T--IISER-Mohali-INDIA--CS4.png" alt="Flow chart"></td></tr></table></center> | <center><table><tr><td><img src="https://static.igem.org/mediawiki/2017/3/31/T--IISER-Mohali-INDIA--CS4.png" alt="Flow chart"></td></tr></table></center> | ||
<h4>For cloning of construct 2, Pctx-RBS5- chromoprotein I- RBS6-TetR- terminator5.</h4> | <h4>For cloning of construct 2, Pctx-RBS5- chromoprotein I- RBS6-TetR- terminator5.</h4> |
Revision as of 13:41, 1 November 2017