Difference between revisions of "Team:BNU-China/Safety"

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{{BNU-China}}
 
 
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    <title>BNU-China</title>
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    <meta name="Team" content="BNU-China" />
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    <meta name="keywords" content="iGEM 2017" />
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    <meta name="author" content="Ziyu Liu（刘梓钰）,Zhaodong Wang（王兆栋）,Xizong Zhang（张溪棕）" />
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    <link type="text/ttf" rel="stylesheet" href="https://2017.igem.org/File:T--BNU-China--CiceroSans.ttf" />
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    <link type="text/ttf" rel="stylesheet" href="https://2017.igem.org/File:T--BNU-China--Regular.ttf" />
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#content{
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#safe-operation {
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#safe-operation>li>p {
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<h1> Safety </h1>
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        <img src="https://static.igem.org/mediawiki/2017/6/60/T--BNU-China--index2.1.png" alt="Sorry, the image is not spupported by your browser.">
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<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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        <img id="eye" src="https://static.igem.org/mediawiki/2017/5/53/T--BNU-China--index1.jpg" alt="Sorry, the image is not spupported by your browser." style="width:100;position:absolute;top:-3px;">
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        <ul>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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            <li id="w_Project"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Description">Project</a></li>
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            <li id="w_Modeling"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Model">Modeling</a></li>
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            <li id="w_Practice"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/HP">Practice</a></li>
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            <li id="w_home"><a class="w_a" style="opacity:0;" href="https://2017.igem.org/Team:BNU-China">Home</a></li>
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            <li id="w_Achievement"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Judging">Achievement</a></li>
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            <li id="w_Lab"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Team">Lab</a></li>
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<h5>Safe Project Design</h5>
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            <li id="w_Safety"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Safety">Safety</a></li>
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        </ul>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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        <div id="w_navMenu" style="text-align:right;">
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            <p>Menu▼</p>
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<li>Choosing a non-pathogenic chassis</li>
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                <li id="w_Project"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Description">Project</a></li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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                <li id="w_Modeling"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Model">Modeling</a></li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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                <li id="w_Practice"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/HP">Practice</a></li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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                <li id="w_Achievement"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Judging">Achievement</a></li>
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                <li id="w_Lab"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Team">Lab</a></li>
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                <li id="w_Safety"><a class="w_a" href="https://2017.igem.org/Team:BNU-China/Safety">Safety</a></li>
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    <div id="w_content" style="width:100%;">
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        <div id="w_Panel" style="text-align:left;">
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            <ul id="w_menu">
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                <li id="w_menu1">
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                    <a class="w_a" href="https://2017.igem.org/Team:BNU-China/Safety">Safety</a>
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                    <ul>
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                        <li><p data-id="#title1">Biomaterial Overview</p></li>
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                        <li><p data-id="#title2">Measures</p></li>
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            <h1>Safety</h1>
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<h2 id="title1">Biomaterial Overview</h2>
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<p>Our project aims to improve the yeast surface display system by assemble biopolymers on the yeast cell wall. Here are two types of biopolymers synthesized our chasis organisms, the engineered <i>S. cerevisiae str. EBY100</i> and <i>INVSc1</i>. One is tubulin protein, whose sequences originate in <i>Mus musculus</i> (house mouse). The other one is flagellin protein coded by codon optimized sequences of <i>E.coli str. K-12</i>. In addition, there are some enzymes fused with the subunits of the biopolymers, including PETase, Xynase, BG, EG, CBH. All of the organisms involved, both the chasis organisms and those we acquire original coding sequences from, are belonged to the risk group one, which do not cause disease in healthy adult humans. </p>
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<h2 id="title2">Measures</h2>
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<p>Even though the organisms of our choice are relatively safe ones, we still took necessary measures to guarantee the safety of our project. </p>
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<p>First of all, we strictly limited the engineered strain within the lab by destroying their ability to survive under environment lack of certain substances. The engineered <i>EBY100</i> cells cannot sustain deficiency in leucine, and the engineered <i>INVSc1</i> cannot survive without histidine, tryptophan, and leucine.</p>
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<p>Secondly, before starting our lab work, we thoroughly examined the safety protection facilities, including wall-mounted eye-wash and emergency shower, first aid kits, fire extinguishers, etc. The broken, dysfunctional, overdue ones were immediately fixed or replaced.</p>
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<p>Thirdly, to keep the risk of accidents and leakages to the lowest level, we strictly followed the rules and codes of safe operation listed below: </p>
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<ul id="safe-operation">
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    <li><p style="padding-left:7px;">No slippers and pants would be permitted in lab. Close-toed shoes are worn at all times in the lab.</p></li>
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    <li><p style="padding-left:7px;">Gloves and medical mask is ordered when working in the labs, especially with some aerosolizing agents, such as SDS.</p></li>
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    <li><p style="padding-left:7px;">Hands must be washed before leaving the lab area. And lab coats are mandatory precaution.</p></li>  
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    <li><p style="padding-left:7px;">No food or drink are allowed in the lab.</p></li>
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    <li><p style="padding-left:7px;">All bench space is wiped with 75% EtOH before and after use, and the biosafety cabinets are exposed to UV for twenty minutes before and after use.</p></li>
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    <li><p style="padding-left:7px;">All unnecessary equipment is unplugged and removed from bench after use.</p></li>
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    <li><p style="padding-left:7px;">All rules on safety operation on high-risk equipment are strictly followed as documented in our <a href="https://static.igem.org/mediawiki/2017/b/bd/T--BNU-China--video1.mp4">video</a>.</p></li>
 
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<div style="background-color:#F7EDB7;text-align:center;">
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<p style="text-align: center;color: #e5b051;padding-top: 5px;margin-top:75px;">Copyright © 2017 <a href="https://2017.igem.org/Team:BNU-China" style="color: #9E2B20;text-decoration: none;opacity:0.8;">BNU-China</a>&nbsp;All rights reserved.</p>
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<p style="text-align: center;color: #e5b051;margin-bottom:-20px;">If you like this page, you can contact us: <span style="color:#9E2B20;text-decoration: none;opacity:0.8;">bnu_igem@163.com</a></span>
 
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h5>Safe Shipment</h5>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Latest revision as of 14:46, 1 November 2017

BNU-China

  • Safety

    • Biomaterial Overview

    • Measures

Safety

Biomaterial Overview

Our project aims to improve the yeast surface display system by assemble biopolymers on the yeast cell wall. Here are two types of biopolymers synthesized our chasis organisms, the engineered S. cerevisiae str. EBY100 and INVSc1. One is tubulin protein, whose sequences originate in Mus musculus (house mouse). The other one is flagellin protein coded by codon optimized sequences of E.coli str. K-12. In addition, there are some enzymes fused with the subunits of the biopolymers, including PETase, Xynase, BG, EG, CBH. All of the organisms involved, both the chasis organisms and those we acquire original coding sequences from, are belonged to the risk group one, which do not cause disease in healthy adult humans.

Measures

Even though the organisms of our choice are relatively safe ones, we still took necessary measures to guarantee the safety of our project.

First of all, we strictly limited the engineered strain within the lab by destroying their ability to survive under environment lack of certain substances. The engineered EBY100 cells cannot sustain deficiency in leucine, and the engineered INVSc1 cannot survive without histidine, tryptophan, and leucine.

Secondly, before starting our lab work, we thoroughly examined the safety protection facilities, including wall-mounted eye-wash and emergency shower, first aid kits, fire extinguishers, etc. The broken, dysfunctional, overdue ones were immediately fixed or replaced.

Thirdly, to keep the risk of accidents and leakages to the lowest level, we strictly followed the rules and codes of safe operation listed below:

  • No slippers and pants would be permitted in lab. Close-toed shoes are worn at all times in the lab.

  • Gloves and medical mask is ordered when working in the labs, especially with some aerosolizing agents, such as SDS.

  • Hands must be washed before leaving the lab area. And lab coats are mandatory precaution.

  • No food or drink are allowed in the lab.

  • All bench space is wiped with 75% EtOH before and after use, and the biosafety cabinets are exposed to UV for twenty minutes before and after use.

  • All unnecessary equipment is unplugged and removed from bench after use.

  • All rules on safety operation on high-risk equipment are strictly followed as documented in our video.

Copyright © 2017 BNU-China All rights reserved.

If you like this page, you can contact us: bnu_igem@163.com