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− | Lorem ipsum dolor sit amet, consectetur adipisicing elit. Quas sit expedita, iusto repellendus cumque, officia architecto consequatur illo fuga eum sed ut autem eos voluptas. Nemo, a, rem! Atque quisquam aperiam eaque tenetur autem, soluta itaque omnis. Minus nesciunt, sint, animi illum quo ab voluptate esse delectus unde maiores iure, quasi a suscipit ipsam aliquid voluptatem. Perspiciatis eveniet, pariatur illum aut cum dolor neque consequatur error aliquid facilis in quasi temporibus assumenda tempore, doloremque autem saepe enim nihil. Voluptates asperiores ullam voluptate quas similique ratione quia hic, eum distinctio laboriosam, consectetur tempora voluptatibus optio natus cumque est necessitatibus dolore alias. | + | Here, we hope to develop an irreversible biological carbon fixed system through genetic engineering upon a kind of seaweed Synechococcus sp. PCC 7002, which would be used to fix carbon dioxide high efficiently in vast wild nature environment. |
| + | To reach our goal, the algae should be genetically engineered in two directions. First it need to be transformed with some stronger or more efficiency carbon fixation genes like Rubisco or glucogen genes to improve CO2 capturing and conversion. And further it will express metal binding proteins on surface to promote the absorption of metal irons to increase the cell density. Through these modifications, the algae is designed to sink to sea bottom in the late growth stage with the carbon products together so as to reduce the carbon recycle. |
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| + | <video src="https://static.igem.org/mediawiki/2017/1/14/T-Worldshaper-Nanjing-v001.mp4" width="1000" height="" controls="controls"></video> |
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| + | <p> <b>Detail</b> |
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− | Lorem ipsum dolor sit amet, consectetur adipisicing elit. Veritatis expedita repellendus laboriosam aliquid. Neque doloribus ea, id reprehenderit alias saepe debitis eligendi molestias odit, nesciunt rem. Dolorem saepe, provident dolore nesciunt laudantium nostrum enim natus veritatis harum maxime et iure ratione, nulla. Minus excepturi commodi tempore voluptate. Blanditiis similique dolor asperiores ex excepturi perspiciatis, dolores id esse. Voluptate beatae nesciunt cum esse ratione officiis necessitatibus blanditiis ea, laboriosam fugit vero maxime? Voluptatum illo dolorum autem pariatur quisquam. Voluptates soluta culpa necessitatibus veritatis tempora incidunt doloribus placeat repellat et facilis eum sapiente fugit numquam aut, laboriosam aspernatur, esse, magnam excepturi repudiandae amet voluptas nulla quidem. Veritatis nisi consequuntur saepe qui quisquam dignissimos assumenda, iusto odio. Dignissimos reprehenderit esse iusto cupiditate nisi enim, animi similique itaque, perspiciatis error qui. Aperiam, architecto provident. | + | Although it is a big and long-time project with lots of work to do and with great challenges, we have already moved forword with several critical and important steps in the past one year. These works can be divided into five major parts. The part 1 is about choosing a perfect algae from lots of candidates. In Part2 we mainly described the design and construction of expression vectors. In part3 we tested the metal binding using surface display in E.coli, which is a critical and fundamental work of ‘sinking alga’. And part 4 is about the transgenetic engineering of Synechococcus sp. PCC 7002. Finally we introduced a prototype of culture device designed by our team. |
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