Difference between revisions of "Team:UFlorida/Experiments"

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<h1 style="text-align:center">Analysis</h1>
 
<h1 style="text-align:center">Analysis</h1>

Revision as of 14:59, 1 November 2017

HPLC Conditions

Eluent A

2.5 : 97.5 % (v/v) acetic acid: H2O, pH 3.8

*pH was adjusted by addition of 1 mol L-1 KOH

Eluent B

80 : 20 % (v/v) acetonitrile: H2O

Gradient Elution (A>B)

80:20

50:50

0:100

80:20

Time Scale(min) @ FR (1ml/min)

0-25

25-31

31-33

33-36


Analysis

Initial Standard Run of Tryptophan

MW of compounds:
Tryptophan MM: 204.225 g/mol
Indole-3-pyruvic acid MM: 203.197 g/mol
Indole-3-acetaldehyde MM: 159.188 g/mol
Tryptophol MM: 161.20 g/mol

Tryptophan and Indole-3-pyruvic acid

Tryptophan and indole-3-pyruvic acid are too close in MM that unintended reactions during the run would make it difficult to differentiate the two.