Difference between revisions of "Team:UFlorida/Experiments"
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<h3 style="text-align:center">Initial Standard Run of Tryptophan</h3> | <h3 style="text-align:center">Initial Standard Run of Tryptophan</h3> | ||
| − | MW of compounds: | + | <p style="text-align:center">MW of compounds: |
<br> | <br> | ||
Tryptophan MM: 204.225 g/mol | Tryptophan MM: 204.225 g/mol | ||
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Indole-3-acetaldehyde MM: 159.188 g/mol | Indole-3-acetaldehyde MM: 159.188 g/mol | ||
<br> | <br> | ||
| − | Tryptophol MM: 161.20 g/mol | + | Tryptophol MM: 161.20 g/mol </p> |
<h3 style="text-align:center">Tryptophan and Indole-3-pyruvic acid</h3> | <h3 style="text-align:center">Tryptophan and Indole-3-pyruvic acid</h3> | ||
Tryptophan and indole-3-pyruvic acid are too close in MM that unintended reactions during the run would make it difficult to differentiate the two. | Tryptophan and indole-3-pyruvic acid are too close in MM that unintended reactions during the run would make it difficult to differentiate the two. | ||
</html> | </html> | ||
Revision as of 15:00, 1 November 2017
HPLC Conditions
Eluent A
2.5 : 97.5 % (v/v) acetic acid: H2O, pH 3.8
*pH was adjusted by addition of 1 mol L-1 KOH
Eluent B
80 : 20 % (v/v) acetonitrile: H2O
Gradient Elution (A>B)
80:20
50:50
0:100
80:20
Time Scale(min) @ FR (1ml/min)
0-25
25-31
31-33
33-36
Analysis
Initial Standard Run of Tryptophan
MW of compounds:
Tryptophan MM: 204.225 g/mol
Indole-3-pyruvic acid MM: 203.197 g/mol
Indole-3-acetaldehyde MM: 159.188 g/mol
Tryptophol MM: 161.20 g/mol
