Difference between revisions of "Team:DEIAGRA/InterLab"

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mso-font-charset:0;
 +
mso-generic-font-family:swiss;
 +
mso-font-pitch:variable;
 +
mso-font-signature:-520092929 1073786111 9 0 415 0;}
 +
@font-face
 +
{font-family:Tahoma;
 +
panose-1:2 11 6 4 3 5 4 4 2 4;
 +
mso-font-charset:0;
 +
mso-generic-font-family:swiss;
 +
mso-font-pitch:variable;
 +
mso-font-signature:-520081665 -1073717157 41 0 66047 0;}
 +
@font-face
 +
{font-family:"Arial Black";
 +
panose-1:2 11 10 4 2 1 2 2 2 4;
 +
mso-font-charset:0;
 +
mso-generic-font-family:swiss;
 +
mso-font-pitch:variable;
 +
mso-font-signature:647 0 0 0 159 0;}
 +
/* Style Definitions */
 +
p.MsoNormal, li.MsoNormal, div.MsoNormal
 +
{mso-style-unhide:no;
 +
mso-style-qformat:yes;
 +
mso-style-parent:"";
 +
margin-top:0in;
 +
margin-right:0in;
 +
margin-bottom:10.0pt;
 +
margin-left:0in;
 +
line-height:115%;
 +
mso-pagination:widow-orphan;
 +
font-size:11.0pt;
 +
font-family:"Calibri","sans-serif";
 +
mso-ascii-font-family:Calibri;
 +
mso-ascii-theme-font:minor-latin;
 +
mso-fareast-font-family:Calibri;
 +
mso-fareast-theme-font:minor-latin;
 +
mso-hansi-font-family:Calibri;
 +
mso-hansi-theme-font:minor-latin;
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-bidi-theme-font:minor-bidi;}
 +
h1
 +
{mso-style-priority:9;
 +
mso-style-unhide:no;
 +
mso-style-qformat:yes;
 +
mso-style-link:"Heading 1 Char";
 +
mso-margin-top-alt:auto;
 +
margin-right:0in;
 +
mso-margin-bottom-alt:auto;
 +
margin-left:0in;
 +
mso-pagination:widow-orphan;
 +
mso-outline-level:1;
 +
font-size:24.0pt;
 +
font-family:"Times New Roman","serif";
 +
mso-fareast-font-family:"Times New Roman";
 +
font-weight:bold;}
 +
h2
 +
{mso-style-noshow:yes;
 +
mso-style-priority:9;
 +
mso-style-qformat:yes;
 +
mso-style-link:"Heading 2 Char";
 +
mso-style-next:Normal;
 +
margin-top:10.0pt;
 +
margin-right:0in;
 +
margin-bottom:0in;
 +
margin-left:0in;
 +
margin-bottom:.0001pt;
 +
line-height:115%;
 +
mso-pagination:widow-orphan lines-together;
 +
page-break-after:avoid;
 +
mso-outline-level:2;
 +
font-size:13.0pt;
 +
font-family:"Cambria","serif";
 +
mso-ascii-font-family:Cambria;
 +
mso-ascii-theme-font:major-latin;
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:major-fareast;
 +
mso-hansi-font-family:Cambria;
 +
mso-hansi-theme-font:major-latin;
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-bidi-theme-font:major-bidi;
 +
color:#4F81BD;
 +
mso-themecolor:accent1;
 +
font-weight:bold;}
 +
h3
 +
{mso-style-noshow:yes;
 +
mso-style-priority:9;
 +
mso-style-qformat:yes;
 +
mso-style-link:"Heading 3 Char";
 +
mso-style-next:Normal;
 +
margin-top:10.0pt;
 +
margin-right:0in;
 +
margin-bottom:0in;
 +
margin-left:0in;
 +
margin-bottom:.0001pt;
 +
line-height:115%;
 +
mso-pagination:widow-orphan lines-together;
 +
page-break-after:avoid;
 +
mso-outline-level:3;
 +
font-size:11.0pt;
 +
font-family:"Cambria","serif";
 +
mso-ascii-font-family:Cambria;
 +
mso-ascii-theme-font:major-latin;
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:major-fareast;
 +
mso-hansi-font-family:Cambria;
 +
mso-hansi-theme-font:major-latin;
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-bidi-theme-font:major-bidi;
 +
color:#4F81BD;
 +
mso-themecolor:accent1;
 +
font-weight:bold;}
 +
a:link, span.MsoHyperlink
 +
{mso-style-priority:99;
 +
color:blue;
 +
mso-themecolor:hyperlink;
 +
text-decoration:underline;
 +
text-underline:single;}
 +
a:visited, span.MsoHyperlinkFollowed
 +
{mso-style-noshow:yes;
 +
mso-style-priority:99;
 +
color:purple;
 +
mso-themecolor:followedhyperlink;
 +
text-decoration:underline;
 +
text-underline:single;}
 +
p
 +
{mso-style-noshow:yes;
 +
mso-style-priority:99;
 +
mso-margin-top-alt:auto;
 +
margin-right:0in;
 +
mso-margin-bottom-alt:auto;
 +
margin-left:0in;
 +
mso-pagination:widow-orphan;
 +
font-size:12.0pt;
 +
font-family:"Times New Roman","serif";
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:minor-fareast;}
 +
p.MsoAcetate, li.MsoAcetate, div.MsoAcetate
 +
{mso-style-noshow:yes;
 +
mso-style-priority:99;
 +
mso-style-link:"Balloon Text Char";
 +
margin:0in;
 +
margin-bottom:.0001pt;
 +
mso-pagination:widow-orphan;
 +
font-size:8.0pt;
 +
font-family:"Tahoma","sans-serif";
 +
mso-fareast-font-family:Calibri;
 +
mso-fareast-theme-font:minor-latin;}
 +
p.MsoListParagraph, li.MsoListParagraph, div.MsoListParagraph
 +
{mso-style-priority:34;
 +
mso-style-unhide:no;
 +
mso-style-qformat:yes;
 +
margin-top:0in;
 +
margin-right:0in;
 +
margin-bottom:10.0pt;
 +
margin-left:.5in;
 +
mso-add-space:auto;
 +
line-height:115%;
 +
mso-pagination:widow-orphan;
 +
font-size:11.0pt;
 +
font-family:"Calibri","sans-serif";
 +
mso-ascii-font-family:Calibri;
 +
mso-ascii-theme-font:minor-latin;
 +
mso-fareast-font-family:Calibri;
 +
mso-fareast-theme-font:minor-latin;
 +
mso-hansi-font-family:Calibri;
 +
mso-hansi-theme-font:minor-latin;
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-bidi-theme-font:minor-bidi;}
 +
p.MsoListParagraphCxSpFirst, li.MsoListParagraphCxSpFirst, div.MsoListParagraphCxSpFirst
 +
{mso-style-priority:34;
 +
mso-style-unhide:no;
 +
mso-style-qformat:yes;
 +
mso-style-type:export-only;
 +
margin-top:0in;
 +
margin-right:0in;
 +
margin-bottom:0in;
 +
margin-left:.5in;
 +
margin-bottom:.0001pt;
 +
mso-add-space:auto;
 +
line-height:115%;
 +
mso-pagination:widow-orphan;
 +
font-size:11.0pt;
 +
font-family:"Calibri","sans-serif";
 +
mso-ascii-font-family:Calibri;
 +
mso-ascii-theme-font:minor-latin;
 +
mso-fareast-font-family:Calibri;
 +
mso-fareast-theme-font:minor-latin;
 +
mso-hansi-font-family:Calibri;
 +
mso-hansi-theme-font:minor-latin;
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-bidi-theme-font:minor-bidi;}
 +
p.MsoListParagraphCxSpMiddle, li.MsoListParagraphCxSpMiddle, div.MsoListParagraphCxSpMiddle
 +
{mso-style-priority:34;
 +
mso-style-unhide:no;
 +
mso-style-qformat:yes;
 +
mso-style-type:export-only;
 +
margin-top:0in;
 +
margin-right:0in;
 +
margin-bottom:0in;
 +
margin-left:.5in;
 +
margin-bottom:.0001pt;
 +
mso-add-space:auto;
 +
line-height:115%;
 +
mso-pagination:widow-orphan;
 +
font-size:11.0pt;
 +
font-family:"Calibri","sans-serif";
 +
mso-ascii-font-family:Calibri;
 +
mso-ascii-theme-font:minor-latin;
 +
mso-fareast-font-family:Calibri;
 +
mso-fareast-theme-font:minor-latin;
 +
mso-hansi-font-family:Calibri;
 +
mso-hansi-theme-font:minor-latin;
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-bidi-theme-font:minor-bidi;}
 +
p.MsoListParagraphCxSpLast, li.MsoListParagraphCxSpLast, div.MsoListParagraphCxSpLast
 +
{mso-style-priority:34;
 +
mso-style-unhide:no;
 +
mso-style-qformat:yes;
 +
mso-style-type:export-only;
 +
margin-top:0in;
 +
margin-right:0in;
 +
margin-bottom:10.0pt;
 +
margin-left:.5in;
 +
mso-add-space:auto;
 +
line-height:115%;
 +
mso-pagination:widow-orphan;
 +
font-size:11.0pt;
 +
font-family:"Calibri","sans-serif";
 +
mso-ascii-font-family:Calibri;
 +
mso-ascii-theme-font:minor-latin;
 +
mso-fareast-font-family:Calibri;
 +
mso-fareast-theme-font:minor-latin;
 +
mso-hansi-font-family:Calibri;
 +
mso-hansi-theme-font:minor-latin;
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-bidi-theme-font:minor-bidi;}
 +
span.BalloonTextChar
 +
{mso-style-name:"Balloon Text Char";
 +
mso-style-noshow:yes;
 +
mso-style-priority:99;
 +
mso-style-unhide:no;
 +
mso-style-locked:yes;
 +
mso-style-link:"Balloon Text";
 +
mso-ansi-font-size:8.0pt;
 +
mso-bidi-font-size:8.0pt;
 +
font-family:"Tahoma","sans-serif";
 +
mso-ascii-font-family:Tahoma;
 +
mso-hansi-font-family:Tahoma;
 +
mso-bidi-font-family:Tahoma;}
 +
span.Heading1Char
 +
{mso-style-name:"Heading 1 Char";
 +
mso-style-priority:9;
 +
mso-style-unhide:no;
 +
mso-style-locked:yes;
 +
mso-style-link:"Heading 1";
 +
mso-ansi-font-size:24.0pt;
 +
mso-bidi-font-size:24.0pt;
 +
font-family:"Times New Roman","serif";
 +
mso-ascii-font-family:"Times New Roman";
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-hansi-font-family:"Times New Roman";
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-font-kerning:18.0pt;
 +
font-weight:bold;}
 +
span.Heading2Char
 +
{mso-style-name:"Heading 2 Char";
 +
mso-style-noshow:yes;
 +
mso-style-priority:9;
 +
mso-style-unhide:no;
 +
mso-style-locked:yes;
 +
mso-style-link:"Heading 2";
 +
mso-ansi-font-size:13.0pt;
 +
mso-bidi-font-size:13.0pt;
 +
font-family:"Cambria","serif";
 +
mso-ascii-font-family:Cambria;
 +
mso-ascii-theme-font:major-latin;
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:major-fareast;
 +
mso-hansi-font-family:Cambria;
 +
mso-hansi-theme-font:major-latin;
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-bidi-theme-font:major-bidi;
 +
color:#4F81BD;
 +
mso-themecolor:accent1;
 +
font-weight:bold;}
 +
span.Heading3Char
 +
{mso-style-name:"Heading 3 Char";
 +
mso-style-noshow:yes;
 +
mso-style-priority:9;
 +
mso-style-unhide:no;
 +
mso-style-locked:yes;
 +
mso-style-link:"Heading 3";
 +
font-family:"Cambria","serif";
 +
mso-ascii-font-family:Cambria;
 +
mso-ascii-theme-font:major-latin;
 +
mso-fareast-font-family:"Times New Roman";
 +
mso-fareast-theme-font:major-fareast;
 +
mso-hansi-font-family:Cambria;
 +
mso-hansi-theme-font:major-latin;
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-bidi-theme-font:major-bidi;
 +
color:#4F81BD;
 +
mso-themecolor:accent1;
 +
font-weight:bold;}
 +
.MsoChpDefault
 +
{mso-style-type:export-only;
 +
mso-default-props:yes;
 +
font-family:"Calibri","sans-serif";
 +
mso-ascii-font-family:Calibri;
 +
mso-ascii-theme-font:minor-latin;
 +
mso-fareast-font-family:Calibri;
 +
mso-fareast-theme-font:minor-latin;
 +
mso-hansi-font-family:Calibri;
 +
mso-hansi-theme-font:minor-latin;
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-bidi-theme-font:minor-bidi;}
 +
.MsoPapDefault
 +
{mso-style-type:export-only;
 +
margin-bottom:10.0pt;
 +
line-height:115%;}
 +
@page WordSection1
 +
{size:8.5in 11.0in;
 +
margin:1.0in 1.0in 1.0in 1.0in;
 +
mso-header-margin:.5in;
 +
mso-footer-margin:.5in;
 +
mso-paper-source:0;}
 +
div.WordSection1
 +
{page:WordSection1;}
 +
/* List Definitions */
 +
@list l0
 +
{mso-list-id:1222712662;
 +
mso-list-type:hybrid;
 +
mso-list-template-ids:-1517373948 -2078644262 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
 +
@list l0:level1
 +
{mso-level-tab-stop:none;
 +
mso-level-number-position:left;
 +
margin-left:39.0pt;
 +
text-indent:-.5in;
 +
mso-ansi-font-size:11.0pt;
 +
font-family:"Times New Roman","serif";
 +
mso-ascii-font-family:Calibri;
 +
mso-ascii-theme-font:minor-latin;
 +
mso-fareast-font-family:Calibri;
 +
mso-fareast-theme-font:minor-latin;
 +
mso-hansi-font-family:Calibri;
 +
mso-hansi-theme-font:minor-latin;
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-bidi-theme-font:minor-bidi;
 +
mso-ansi-font-weight:normal;}
 +
@list l0:level2
 +
{mso-level-number-format:alpha-lower;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:left;
 +
margin-left:57.0pt;
 +
text-indent:-.25in;}
 +
@list l0:level3
 +
{mso-level-number-format:roman-lower;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:right;
 +
margin-left:93.0pt;
 +
text-indent:-9.0pt;}
 +
@list l0:level4
 +
{mso-level-tab-stop:none;
 +
mso-level-number-position:left;
 +
margin-left:129.0pt;
 +
text-indent:-.25in;}
 +
@list l0:level5
 +
{mso-level-number-format:alpha-lower;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:left;
 +
margin-left:165.0pt;
 +
text-indent:-.25in;}
 +
@list l0:level6
 +
{mso-level-number-format:roman-lower;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:right;
 +
margin-left:201.0pt;
 +
text-indent:-9.0pt;}
 +
@list l0:level7
 +
{mso-level-tab-stop:none;
 +
mso-level-number-position:left;
 +
margin-left:237.0pt;
 +
text-indent:-.25in;}
 +
@list l0:level8
 +
{mso-level-number-format:alpha-lower;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:left;
 +
margin-left:273.0pt;
 +
text-indent:-.25in;}
 +
@list l0:level9
 +
{mso-level-number-format:roman-lower;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:right;
 +
margin-left:309.0pt;
 +
text-indent:-9.0pt;}
 +
@list l1
 +
{mso-list-id:1241453227;
 +
mso-list-type:hybrid;
 +
mso-list-template-ids:1146023568 1452603116 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
 +
@list l1:level1
 +
{mso-level-start-at:3;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:left;
 +
margin-left:57.0pt;
 +
text-indent:-.25in;}
 +
@list l1:level2
 +
{mso-level-number-format:alpha-lower;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:left;
 +
margin-left:93.0pt;
 +
text-indent:-.25in;}
 +
@list l1:level3
 +
{mso-level-number-format:roman-lower;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:right;
 +
margin-left:129.0pt;
 +
text-indent:-9.0pt;}
 +
@list l1:level4
 +
{mso-level-tab-stop:none;
 +
mso-level-number-position:left;
 +
margin-left:165.0pt;
 +
text-indent:-.25in;}
 +
@list l1:level5
 +
{mso-level-number-format:alpha-lower;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:left;
 +
margin-left:201.0pt;
 +
text-indent:-.25in;}
 +
@list l1:level6
 +
{mso-level-number-format:roman-lower;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:right;
 +
margin-left:237.0pt;
 +
text-indent:-9.0pt;}
 +
@list l1:level7
 +
{mso-level-tab-stop:none;
 +
mso-level-number-position:left;
 +
margin-left:273.0pt;
 +
text-indent:-.25in;}
 +
@list l1:level8
 +
{mso-level-number-format:alpha-lower;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:left;
 +
margin-left:309.0pt;
 +
text-indent:-.25in;}
 +
@list l1:level9
 +
{mso-level-number-format:roman-lower;
 +
mso-level-tab-stop:none;
 +
mso-level-number-position:right;
 +
margin-left:345.0pt;
 +
text-indent:-9.0pt;}
 +
ol
 +
{margin-bottom:0in;}
 +
ul
 +
{margin-bottom:0in;}
 +
-->
 +
</style>
 +
<!--[if gte mso 10]>
 +
<style>
 +
/* Style Definitions */
 +
table.MsoNormalTable
 +
{mso-style-name:"Table Normal";
 +
mso-tstyle-rowband-size:0;
 +
mso-tstyle-colband-size:0;
 +
mso-style-noshow:yes;
 +
mso-style-priority:99;
 +
mso-style-parent:"";
 +
mso-padding-alt:0in 5.4pt 0in 5.4pt;
 +
mso-para-margin-top:0in;
 +
mso-para-margin-right:0in;
 +
mso-para-margin-bottom:10.0pt;
 +
mso-para-margin-left:0in;
 +
line-height:115%;
 +
mso-pagination:widow-orphan;
 +
font-size:11.0pt;
 +
font-family:"Calibri","sans-serif";
 +
mso-ascii-font-family:Calibri;
 +
mso-ascii-theme-font:minor-latin;
 +
mso-hansi-font-family:Calibri;
 +
mso-hansi-theme-font:minor-latin;
 +
mso-bidi-font-family:"Times New Roman";
 +
mso-bidi-theme-font:minor-bidi;}
 +
table.MsoTableGrid
 +
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</head>
  
 +
<body lang=EN-US link=blue vlink=purple style='tab-interval:.5in'>
  
−
<div class="clear"></div>
+
<div class=WordSection1>
  
−
<div class="column full_size">
+
<p class=MsoNormal align=center style='margin-bottom:0in;margin-bottom:.0001pt;
−
<h1>InterLab</h1>
+
text-align:center;line-height:normal;mso-outline-level:1;background:white'><b><span
−
<h3>Bronze Medal Criterion #4</h3>
+
style='font-size:28.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
−
<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range.
+
"Times New Roman";mso-font-kerning:18.0pt'>Fourth International Inter-Lab
−
<br><br>
+
Measurement Study<o:p></o:p></span></b></p>
−
For teams participating in the <a href="https://2017.igem.org/Competition/InterLab_Study">InterLab study</a>, all work must be shown on this page.
+
  
−
</p>
+
<p class=MsoNormal align=center style='margin-bottom:0in;margin-bottom:.0001pt;
−
</div>
+
text-align:center;line-height:normal;mso-outline-level:1;background:white'><b><span
 +
style='font-size:28.0pt;font-family:"Arial","sans-serif";mso-fareast-font-family:
 +
"Times New Roman";mso-font-kerning:18.0pt'><o:p>&nbsp;</o:p></span></b></p>
  
 +
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 +
normal;mso-outline-level:1;background:white'><o:p>&nbsp;</o:p></p>
  
 +
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;text-align:
 +
justify;line-height:150%;mso-outline-level:1;background:white'><span
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif";
 +
color:black;background:white'>This year Inter-lab Measurement Study aims to establish
 +
a GFP measurement protocol based on engineering principles for testing six RBS
 +
devices (BCDs) that are intended to make gene expression more precise and
 +
reliable<span style='mso-spacerun:yes'>  </span>in labs around the world.<o:p></o:p></span></p>
  
 +
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;text-align:
 +
justify;line-height:150%;mso-outline-level:1;background:white'><span
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif";
 +
color:black;background:white'>Team DEIAGRA had followed the plate reader
 +
protocol for this study as mentioned on (</span><a
 +
href="https://static.igem.org/mediawiki/2017/8/85/InterLab_2017_Plate_Reader_Protocol.pdf"><span
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif";
 +
background:white'>https://static.igem.org/mediawiki/2017/8/85/InterLab_2017_Plate_Reader_Protocol.pdf</span></a><span
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif";
 +
color:black;background:white'>) for obtaining the data represented below in
 +
form of graphs. <o:p></o:p></span></p>
  
 +
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;text-align:
 +
justify;line-height:150%;mso-outline-level:1;background:white'><span
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman","serif";
 +
color:black;background:white'><o:p>&nbsp;</o:p></span></p>
  
 +
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;text-align:
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justify;line-height:normal;mso-outline-level:1;background:white'><span
 +
style='font-size:10.0pt;font-family:"Arial Black","sans-serif";mso-bidi-font-family:
 +
Tahoma;color:black;background:white'><o:p>&nbsp;</o:p></span></p>
 +
 +
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
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normal;mso-outline-level:1;background:white'><span style='font-size:10.0pt;
 +
font-family:"Arial Black","sans-serif";mso-bidi-font-family:Tahoma;color:black;
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background:white'><o:p>&nbsp;</o:p></span></p>
 +
 +
<p class=MsoListParagraph style='margin-top:0in;margin-right:0in;margin-bottom:
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0in;margin-left:39.0pt;margin-bottom:.0001pt;mso-add-space:auto;text-indent:
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 +
18.0pt;mso-bidi-font-weight:bold'><span style='mso-list:Ignore'>1.<span
 +
style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><b style='mso-bidi-font-weight:normal'><span
 +
style='font-size:18.0pt;font-family:"Arial Black","sans-serif";mso-bidi-font-family:
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"Times New Roman"'>OD</span></b><b style='mso-bidi-font-weight:normal'><span
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style='font-size:18.0pt;font-family:"Arial","sans-serif"'>&#8203;</span></b><b
 +
style='mso-bidi-font-weight:normal'><span style='font-size:18.0pt;font-family:
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"Arial Black","sans-serif";mso-bidi-font-family:"Times New Roman"'>600</span></b><b
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style='mso-bidi-font-weight:normal'><span style='font-size:18.0pt;font-family:
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"Arial","sans-serif"'>&#8203;</span></b><b style='mso-bidi-font-weight:normal'><span
 +
style='font-size:18.0pt;font-family:"Arial Black","sans-serif";mso-bidi-font-family:
 +
"Times New Roman"'> Reference point</span></b><b><span style='font-size:18.0pt;
 +
font-family:"Arial Black","sans-serif";mso-fareast-font-family:"Times New Roman";
 +
mso-bidi-font-family:"Times New Roman";mso-font-kerning:18.0pt'><o:p></o:p></span></b></p>
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normal;mso-outline-level:1;background:white'><b><span style='font-size:22.0pt;
 +
font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
 +
mso-font-kerning:18.0pt'><o:p>&nbsp;</o:p></span></b></p>
 +
 +
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
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normal;mso-outline-level:1;background:white'><b><span style='font-size:22.0pt;
 +
font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
 +
mso-font-kerning:18.0pt'><o:p>&nbsp;</o:p></span></b></p>
 +
 +
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width=258 style='width:193.85pt;border-collapse:collapse;border:none;
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mso-border-alt:solid windowtext .5pt;mso-yfti-tbllook:1184;mso-table-lspace:
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 +
left;mso-table-top:428.6pt;mso-padding-alt:0in 5.4pt 0in 5.4pt'>
 +
<tr style='mso-yfti-irow:0;mso-yfti-firstrow:yes;height:15.0pt'>
 +
  <td width=111 nowrap valign=top style='width:83.0pt;border:solid windowtext 1.0pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0in 5.4pt 0in 5.4pt;height:15.0pt'>
 +
  <p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
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  mso-element-top:428.6pt;mso-height-rule:exactly'><span style='mso-ascii-font-family:
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  Calibri;mso-fareast-font-family:"Times New Roman";mso-hansi-font-family:Calibri;
 +
  mso-bidi-font-family:Calibri;color:black'><o:p>&nbsp;</o:p></span></p>
 +
  </td>
 +
  <td width=78 nowrap valign=top style='width:58.2pt;border:solid windowtext 1.0pt;
 +
  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
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  solid windowtext .5pt;padding:0in 5.4pt 0in 5.4pt;height:15.0pt'>
 +
  <p class=MsoNormal align=right style='margin-bottom:0in;margin-bottom:.0001pt;
 +
  text-align:right;line-height:normal;mso-element:frame;mso-element-frame-hspace:
 +
  9.0pt;mso-element-wrap:around;mso-element-anchor-vertical:page;mso-element-anchor-horizontal:
 +
  margin;mso-element-top:428.6pt;mso-height-rule:exactly'><span
 +
  style='mso-ascii-font-family:Calibri;mso-fareast-font-family:"Times New Roman";
 +
  mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri;color:black'>LUDOX<o:p></o:p></span></p>
 +
  </td>
 +
  <td width=70 nowrap valign=top style='width:52.65pt;border:solid windowtext 1.0pt;
 +
  border-left:none;mso-border-left-alt:solid windowtext .5pt;mso-border-alt:
 +
  solid windowtext .5pt;padding:0in 5.4pt 0in 5.4pt;height:15.0pt'>
 +
  <p class=MsoNormal align=right style='margin-bottom:0in;margin-bottom:.0001pt;
 +
  text-align:right;line-height:normal;mso-element:frame;mso-element-frame-hspace:
 +
  9.0pt;mso-element-wrap:around;mso-element-anchor-vertical:page;mso-element-anchor-horizontal:
 +
  margin;mso-element-top:428.6pt;mso-height-rule:exactly'><span
 +
  style='mso-ascii-font-family:Calibri;mso-fareast-font-family:"Times New Roman";
 +
  mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri;color:black'>H<sub>2</sub>O<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:1;height:15.0pt'>
 +
  <td width=111 nowrap valign=top style='width:83.0pt;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0in 5.4pt 0in 5.4pt;height:15.0pt'>
 +
  <p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 +
  normal;mso-element:frame;mso-element-frame-hspace:9.0pt;mso-element-wrap:
 +
  around;mso-element-anchor-vertical:page;mso-element-anchor-horizontal:margin;
 +
  mso-element-top:428.6pt;mso-height-rule:exactly'><span style='mso-ascii-font-family:
 +
  Calibri;mso-fareast-font-family:"Times New Roman";mso-hansi-font-family:Calibri;
 +
  mso-bidi-font-family:Calibri;color:black'>Replicate 1<o:p></o:p></span></p>
 +
  </td>
 +
  <td width=78 nowrap valign=top style='width:58.2pt;border-top:none;
 +
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0in 5.4pt 0in 5.4pt;height:15.0pt'>
 +
  <p class=MsoNormal align=right style='margin-bottom:0in;margin-bottom:.0001pt;
 +
  text-align:right;line-height:normal;mso-element:frame;mso-element-frame-hspace:
 +
  9.0pt;mso-element-wrap:around;mso-element-anchor-vertical:page;mso-element-anchor-horizontal:
 +
  margin;mso-element-top:428.6pt;mso-height-rule:exactly'><span
 +
  style='mso-ascii-font-family:Calibri;mso-fareast-font-family:"Times New Roman";
 +
  mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri;color:black'>0.0667<o:p></o:p></span></p>
 +
  </td>
 +
  <td width=70 nowrap valign=top style='width:52.65pt;border-top:none;
 +
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0in 5.4pt 0in 5.4pt;height:15.0pt'>
 +
  <p class=MsoNormal align=right style='margin-bottom:0in;margin-bottom:.0001pt;
 +
  text-align:right;line-height:normal;mso-element:frame;mso-element-frame-hspace:
 +
  9.0pt;mso-element-wrap:around;mso-element-anchor-vertical:page;mso-element-anchor-horizontal:
 +
  margin;mso-element-top:428.6pt;mso-height-rule:exactly'><span
 +
  style='mso-ascii-font-family:Calibri;mso-fareast-font-family:"Times New Roman";
 +
  mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri;color:black'>0.0473<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:2;height:15.0pt'>
 +
  <td width=111 nowrap valign=top style='width:83.0pt;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0in 5.4pt 0in 5.4pt;height:15.0pt'>
 +
  <p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 +
  normal;mso-element:frame;mso-element-frame-hspace:9.0pt;mso-element-wrap:
 +
  around;mso-element-anchor-vertical:page;mso-element-anchor-horizontal:margin;
 +
  mso-element-top:428.6pt;mso-height-rule:exactly'><span style='mso-ascii-font-family:
 +
  Calibri;mso-fareast-font-family:"Times New Roman";mso-hansi-font-family:Calibri;
 +
  mso-bidi-font-family:Calibri;color:black'>Replicate 2<o:p></o:p></span></p>
 +
  </td>
 +
  <td width=78 nowrap valign=top style='width:58.2pt;border-top:none;
 +
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
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  mso-border-alt:solid windowtext .5pt;padding:0in 5.4pt 0in 5.4pt;height:15.0pt'>
 +
  <p class=MsoNormal align=right style='margin-bottom:0in;margin-bottom:.0001pt;
 +
  text-align:right;line-height:normal;mso-element:frame;mso-element-frame-hspace:
 +
  9.0pt;mso-element-wrap:around;mso-element-anchor-vertical:page;mso-element-anchor-horizontal:
 +
  margin;mso-element-top:428.6pt;mso-height-rule:exactly'><span
 +
  style='mso-ascii-font-family:Calibri;mso-fareast-font-family:"Times New Roman";
 +
  mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri;color:black'>0.0617<o:p></o:p></span></p>
 +
  </td>
 +
  <td width=70 nowrap valign=top style='width:52.65pt;border-top:none;
 +
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0in 5.4pt 0in 5.4pt;height:15.0pt'>
 +
  <p class=MsoNormal align=right style='margin-bottom:0in;margin-bottom:.0001pt;
 +
  text-align:right;line-height:normal;mso-element:frame;mso-element-frame-hspace:
 +
  9.0pt;mso-element-wrap:around;mso-element-anchor-vertical:page;mso-element-anchor-horizontal:
 +
  margin;mso-element-top:428.6pt;mso-height-rule:exactly'><span
 +
  style='mso-ascii-font-family:Calibri;mso-fareast-font-family:"Times New Roman";
 +
  mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri;color:black'>0.0489<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:3;height:15.0pt'>
 +
  <td width=111 nowrap valign=top style='width:83.0pt;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0in 5.4pt 0in 5.4pt;height:15.0pt'>
 +
  <p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
 +
  normal;mso-element:frame;mso-element-frame-hspace:9.0pt;mso-element-wrap:
 +
  around;mso-element-anchor-vertical:page;mso-element-anchor-horizontal:margin;
 +
  mso-element-top:428.6pt;mso-height-rule:exactly'><span style='mso-ascii-font-family:
 +
  Calibri;mso-fareast-font-family:"Times New Roman";mso-hansi-font-family:Calibri;
 +
  mso-bidi-font-family:Calibri;color:black'>Replicate 3<o:p></o:p></span></p>
 +
  </td>
 +
  <td width=78 nowrap valign=top style='width:58.2pt;border-top:none;
 +
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0in 5.4pt 0in 5.4pt;height:15.0pt'>
 +
  <p class=MsoNormal align=right style='margin-bottom:0in;margin-bottom:.0001pt;
 +
  text-align:right;line-height:normal;mso-element:frame;mso-element-frame-hspace:
 +
  9.0pt;mso-element-wrap:around;mso-element-anchor-vertical:page;mso-element-anchor-horizontal:
 +
  margin;mso-element-top:428.6pt;mso-height-rule:exactly'><span
 +
  style='mso-ascii-font-family:Calibri;mso-fareast-font-family:"Times New Roman";
 +
  mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri;color:black'>0.0628<o:p></o:p></span></p>
 +
  </td>
 +
  <td width=70 nowrap valign=top style='width:52.65pt;border-top:none;
 +
  border-left:none;border-bottom:solid windowtext 1.0pt;border-right:solid windowtext 1.0pt;
 +
  mso-border-top-alt:solid windowtext .5pt;mso-border-left-alt:solid windowtext .5pt;
 +
  mso-border-alt:solid windowtext .5pt;padding:0in 5.4pt 0in 5.4pt;height:15.0pt'>
 +
  <p class=MsoNormal align=right style='margin-bottom:0in;margin-bottom:.0001pt;
 +
  text-align:right;line-height:normal;mso-element:frame;mso-element-frame-hspace:
 +
  9.0pt;mso-element-wrap:around;mso-element-anchor-vertical:page;mso-element-anchor-horizontal:
 +
  margin;mso-element-top:428.6pt;mso-height-rule:exactly'><span
 +
  style='mso-ascii-font-family:Calibri;mso-fareast-font-family:"Times New Roman";
 +
  mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri;color:black'>0.0515<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr style='mso-yfti-irow:4;height:15.0pt'>
 +
  <td width=111 nowrap valign=top style='width:83.0pt;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
 +
  padding:0in 5.4pt 0in 5.4pt;height:15.0pt'>
 +
  <p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
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  normal;mso-element:frame;mso-element-frame-hspace:9.0pt;mso-element-wrap:
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  around;mso-element-anchor-vertical:page;mso-element-anchor-horizontal:margin;
 +
  mso-element-top:428.6pt;mso-height-rule:exactly'><span style='mso-ascii-font-family:
 +
  Calibri;mso-fareast-font-family:"Times New Roman";mso-hansi-font-family:Calibri;
 +
  mso-bidi-font-family:Calibri;color:black'>Replicate 4<o:p></o:p></span></p>
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  </td>
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  9.0pt;mso-element-wrap:around;mso-element-anchor-vertical:page;mso-element-anchor-horizontal:
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  margin;mso-element-top:428.6pt;mso-height-rule:exactly'><span
 +
  style='mso-ascii-font-family:Calibri;mso-fareast-font-family:"Times New Roman";
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  mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri;color:black'>0.0668<o:p></o:p></span></p>
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  </td>
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  <td width=70 nowrap valign=top style='width:52.65pt;border-top:none;
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  9.0pt;mso-element-wrap:around;mso-element-anchor-vertical:page;mso-element-anchor-horizontal:
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  margin;mso-element-top:428.6pt;mso-height-rule:exactly'><span
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  style='mso-ascii-font-family:Calibri;mso-fareast-font-family:"Times New Roman";
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  mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri;color:black'>0.0506<o:p></o:p></span></p>
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  </td>
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</tr>
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<tr style='mso-yfti-irow:5;height:15.0pt'>
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  <td width=111 nowrap valign=top style='width:83.0pt;border:solid windowtext 1.0pt;
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  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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  padding:0in 5.4pt 0in 5.4pt;height:15.0pt'>
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  <p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
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  mso-element-top:428.6pt;mso-height-rule:exactly'><span style='mso-ascii-font-family:
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  Calibri;mso-fareast-font-family:"Times New Roman";mso-hansi-font-family:Calibri;
 +
  mso-bidi-font-family:Calibri;color:black'>Arith. Mean<o:p></o:p></span></p>
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  </td>
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  <td width=78 nowrap valign=top style='width:58.2pt;border-top:none;
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  <p class=MsoNormal align=right style='margin-bottom:0in;margin-bottom:.0001pt;
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  9.0pt;mso-element-wrap:around;mso-element-anchor-vertical:page;mso-element-anchor-horizontal:
 +
  margin;mso-element-top:428.6pt;mso-height-rule:exactly'><span
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  style='mso-ascii-font-family:Calibri;mso-fareast-font-family:"Times New Roman";
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  mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri;color:black'>0.0645<o:p></o:p></span></p>
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  </td>
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  <td width=70 nowrap valign=top style='width:52.65pt;border-top:none;
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  <p class=MsoNormal align=right style='margin-bottom:0in;margin-bottom:.0001pt;
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  9.0pt;mso-element-wrap:around;mso-element-anchor-vertical:page;mso-element-anchor-horizontal:
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  margin;mso-element-top:428.6pt;mso-height-rule:exactly'><span
 +
  style='mso-ascii-font-family:Calibri;mso-fareast-font-family:"Times New Roman";
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  mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri;color:black'>0.049575<o:p></o:p></span></p>
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  </td>
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</tr>
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<tr style='mso-yfti-irow:6;height:15.0pt'>
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  <td width=111 nowrap valign=top style='width:83.0pt;border:solid windowtext 1.0pt;
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  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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  padding:0in 5.4pt 0in 5.4pt;height:15.0pt'>
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  <p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
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  mso-element-top:428.6pt;mso-height-rule:exactly'><span style='mso-ascii-font-family:
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  Calibri;mso-fareast-font-family:"Times New Roman";mso-hansi-font-family:Calibri;
 +
  mso-bidi-font-family:Calibri;color:black'>Corrected Abs600<o:p></o:p></span></p>
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  </td>
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  <td width=78 nowrap valign=top style='width:58.2pt;border-top:none;
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  <p class=MsoNormal align=right style='margin-bottom:0in;margin-bottom:.0001pt;
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  9.0pt;mso-element-wrap:around;mso-element-anchor-vertical:page;mso-element-anchor-horizontal:
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  margin;mso-element-top:428.6pt;mso-height-rule:exactly'><span
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  style='mso-ascii-font-family:Calibri;mso-fareast-font-family:"Times New Roman";
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  mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri;color:black'>0.014925<o:p></o:p></span></p>
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  </td>
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  mso-border-alt:solid windowtext .5pt;padding:0in 5.4pt 0in 5.4pt;height:15.0pt'></td>
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</tr>
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<tr style='mso-yfti-irow:7;height:15.0pt'>
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  <td width=111 nowrap valign=top style='width:83.0pt;border:solid windowtext 1.0pt;
 +
  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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  padding:0in 5.4pt 0in 5.4pt;height:15.0pt'>
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  <p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
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  mso-element-top:428.6pt;mso-height-rule:exactly'><span style='mso-ascii-font-family:
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  Calibri;mso-fareast-font-family:"Times New Roman";mso-hansi-font-family:Calibri;
 +
  mso-bidi-font-family:Calibri;color:black'>Reference OD600<o:p></o:p></span></p>
 +
  </td>
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  <td width=78 nowrap valign=top style='width:58.2pt;border-top:none;
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  <p class=MsoNormal align=right style='margin-bottom:0in;margin-bottom:.0001pt;
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  9.0pt;mso-element-wrap:around;mso-element-anchor-vertical:page;mso-element-anchor-horizontal:
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  margin;mso-element-top:428.6pt;mso-height-rule:exactly'><span
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  style='mso-ascii-font-family:Calibri;mso-fareast-font-family:"Times New Roman";
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  mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri;color:black'>0.0425<o:p></o:p></span></p>
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  </td>
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</tr>
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<tr style='mso-yfti-irow:8;mso-yfti-lastrow:yes;height:15.0pt'>
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  <td width=111 nowrap valign=top style='width:83.0pt;border:solid windowtext 1.0pt;
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  border-top:none;mso-border-top-alt:solid windowtext .5pt;mso-border-alt:solid windowtext .5pt;
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  Calibri;mso-fareast-font-family:"Times New Roman";mso-hansi-font-family:Calibri;
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  mso-bidi-font-family:Calibri;color:black'>OD600/Abs600<o:p></o:p></span></p>
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  </td>
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  <td width=78 nowrap valign=top style='width:58.2pt;border-top:none;
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  <p class=MsoNormal align=right style='margin-bottom:0in;margin-bottom:.0001pt;
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  margin;mso-element-top:428.6pt;mso-height-rule:exactly'><span
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  style='mso-ascii-font-family:Calibri;mso-fareast-font-family:"Times New Roman";
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  mso-hansi-font-family:Calibri;mso-bidi-font-family:Calibri;color:black'>2.8475712<o:p></o:p></span></p>
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  </td>
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  <td width=70 nowrap valign=top style='width:52.65pt;border-top:none;
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</tr>
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</table>
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normal;mso-outline-level:1;background:white'><b><span style='font-size:22.0pt;
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font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
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mso-font-kerning:18.0pt'><o:p>&nbsp;</o:p></span></b></p>
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<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
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normal;mso-outline-level:1;background:white'><b><span style='font-size:22.0pt;
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font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
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mso-font-kerning:18.0pt'><o:p>&nbsp;</o:p></span></b></p>
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mso-font-kerning:18.0pt'><o:p>&nbsp;</o:p></span></b></p>
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<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
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normal;mso-outline-level:1;background:white'><b><span style='font-size:22.0pt;
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font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
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mso-font-kerning:18.0pt'><o:p>&nbsp;</o:p></span></b></p>
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<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
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normal;mso-outline-level:1;background:white'><b><span style='font-size:22.0pt;
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font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
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mso-font-kerning:18.0pt'><o:p>&nbsp;</o:p></span></b></p>
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font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
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mso-font-kerning:18.0pt'><o:p>&nbsp;</o:p></span></b></p>
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font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
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mso-font-kerning:18.0pt'><o:p>&nbsp;</o:p></span></b></p>
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font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
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mso-font-kerning:18.0pt'><o:p>&nbsp;</o:p></span></b></p>
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normal;mso-outline-level:1;background:white'><b><span style='font-size:22.0pt;
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font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
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mso-font-kerning:18.0pt'><o:p>&nbsp;</o:p></span></b></p>
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<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
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normal;mso-outline-level:1;background:white'><b><span style='font-size:22.0pt;
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font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
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mso-font-kerning:18.0pt'><o:p>&nbsp;</o:p></span></b></p>
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<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
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normal;mso-outline-level:1;background:white'><b><span style='font-size:22.0pt;
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font-family:"Arial","sans-serif";mso-fareast-font-family:"Times New Roman";
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mso-font-kerning:18.0pt'><o:p>&nbsp;</o:p></span></b></p>
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style='font-size:12.0pt;line-height:115%;font-family:"Arial Black","sans-serif";
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color:black;font-weight:normal'>(uM Fluorescein)<o:p></o:p></span></h3>
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<p class=MsoNormal><o:p>&nbsp;</o:p></p>
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<p class=MsoNormal><o:p>&nbsp;</o:p></p>
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<p class=MsoListParagraphCxSpFirst align=center style='margin-left:57.0pt;
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mso-add-space:auto;text-align:center;text-indent:-.25in;mso-list:l1 level1 lfo2'><![if !supportLists]><span
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style='font-size:18.0pt;line-height:115%;font-family:"Arial Black","sans-serif";
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style='mso-list:Ignore'>4.</span></span><![endif]><span style='font-size:18.0pt;
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line-height:115%;font-family:"Arial Black","sans-serif"'>Absorbance Curve<o:p></o:p></span></p>
 +
 +
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Revision as of 15:09, 1 November 2017

Fourth International Inter-Lab Measurement Study

 

 

This year Inter-lab Measurement Study aims to establish a GFP measurement protocol based on engineering principles for testing six RBS devices (BCDs) that are intended to make gene expression more precise and reliable  in labs around the world.

Team DEIAGRA had followed the plate reader protocol for this study as mentioned on (https://static.igem.org/mediawiki/2017/8/85/InterLab_2017_Plate_Reader_Protocol.pdf) for obtaining the data represented below in form of graphs.

 

 

 

1.                   OD​600​ Reference point

 

 

 

LUDOX

H2O

Replicate 1

0.0667

0.0473

Replicate 2

0.0617

0.0489

Replicate 3

0.0628

0.0515

Replicate 4

0.0668

0.0506

Arith. Mean

0.0645

0.049575

Corrected Abs600

0.014925

Reference OD600

0.0425

OD600/Abs600

2.8475712

 

 

 

 

 

 

 

 

 

 

 

2. Fluorescence Standard Curves

(uM Fluorescein)

 

 

 

3.Fluorescence Curve

FluoroscenceCurveReading (Colony 1)

 

 

4.Absorbance Curve

(Optical Density 600 nM)