Difference between revisions of "Team:KUAS Korea/Design"

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<div class="container-fluid page-heading" style="background-image: url(https://static.igem.org/mediawiki/2017/5/5a/T--KUAS_Korea--BG_Design.jpg)">
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    <h3>Design</h3>
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<div class="container-fluid">
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    <div class="row">
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        <div class="col-md-10 col-md-offset-1">
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<h2>POO-robiotics</h2>
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            <div class="section" id="description">
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<br>
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<h1>Design</h1>
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<h4> Detection System 1: HssR/S Mechanism </h4>
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<p>
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Design is the first step in the design-build-test cycle in engineering and synthetic biology. Use this page to describe the process that you used in the design of your parts. You should clearly explain the engineering principles used to design your project.
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<div class="image image-full">
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This page is different to the "Applied Design Award" page. Please see the <a href="https://2017.igem.org/Team:KUAS_Korea/Applied_Design">Applied Design</a> page for more information on how to compete for that award.
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                        <img src="https://static.igem.org/mediawiki/2017/3/35/T--KUAS_Korea--HssRS.png">
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<br><br>
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<p><font size=4>The HssR and HssS mechanism of our project is as follows.</font></p>
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<p><font size=4>      ① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.<br>
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② When heme in the blood binds to the HssS protein, it phosphorylates histidine 249 through autophosphorylation.<br>
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③ HssS transfers the phosphate group from its histidine 249 to aspartate 52 of HssR using transphosphorylation.<br>
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④ The phosphorylated HssR binds to the direct repeat sequence of the hrtAB promoter and initiates the reporter’s transcription.<br>
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      </font><br>
 
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<h4> Detection System 2: HrtR Mechanism </h4>
  
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<h5>What should this page contain?</h5>
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                        <img src="https://static.igem.org/mediawiki/2017/a/a9/T--KUAS_Korea--HrtR.png">
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<ul>
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<br><br>
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<li>Explanation of the engineering principles your team used in your design</li>
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<p><font size=4>Also, the mechanism of heme detection using HrtR protein is as follows.</font></p>
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<li>Discussion of the design iterations your team went through</li>
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<p><font size=4>① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.<br>
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<li>Experimental plan to test your designs</li>
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② When heme binds to the HrtR protein, HrtR binds to the promoter in front of the hrtR sequence and promotes its own transcription.<br>
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③ The reporter behind the hrtR sequence is transcribed together to determine the presence of heme detection.<br>
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      </font><br>
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<h5>Inspiration</h5>
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<ul>
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<li><a href="https://2016.igem.org/Team:MIT/Experiments/Promoters">2016 MIT</a></li>
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<li><a href="https://2016.igem.org/Team:BostonU/Proof">2016 BostonU</a></li>
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<li><a href="https://2016.igem.org/Team:NCTU_Formosa/Design">2016 NCTU Formosa</a></li>
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</ul>
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Latest revision as of 15:17, 1 November 2017

Design

POO-robiotics


Detection System 1: HssR/S Mechanism



The HssR and HssS mechanism of our project is as follows.

① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.
② When heme in the blood binds to the HssS protein, it phosphorylates histidine 249 through autophosphorylation.
③ HssS transfers the phosphate group from its histidine 249 to aspartate 52 of HssR using transphosphorylation.
④ The phosphorylated HssR binds to the direct repeat sequence of the hrtAB promoter and initiates the reporter’s transcription.


Detection System 2: HrtR Mechanism



Also, the mechanism of heme detection using HrtR protein is as follows.

① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.
② When heme binds to the HrtR protein, HrtR binds to the promoter in front of the hrtR sequence and promotes its own transcription.
③ The reporter behind the hrtR sequence is transcribed together to determine the presence of heme detection.