Difference between revisions of "Team:KUAS Korea/Design"
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| − | <div class="container-fluid page-heading" style="background-image: url(https://static.igem.org/mediawiki/ | + | <div class="container-fluid page-heading" style="background-image: url(https://static.igem.org/mediawiki/2017/5/5a/T--KUAS_Korea--BG_Design.jpg)"> |
<h3>Design</h3> | <h3>Design</h3> | ||
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| − | <h4> Detection System 1: | + | <h4> Detection System 1: HssR/S Mechanism </h4> |
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<img src="https://static.igem.org/mediawiki/2017/3/35/T--KUAS_Korea--HssRS.png"> | <img src="https://static.igem.org/mediawiki/2017/3/35/T--KUAS_Korea--HssRS.png"> | ||
| − | <br> | + | <br><br> |
| − | <p><font size=4>The | + | <p><font size=4>The HssR and HssS mechanism of our project is as follows.</font></p> |
<p><font size=4> ① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.<br> | <p><font size=4> ① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.<br> | ||
| − | + | ② When heme in the blood binds to the HssS protein, it phosphorylates histidine 249 through autophosphorylation.<br> | |
| − | + | ③ HssS transfers the phosphate group from its histidine 249 to aspartate 52 of HssR using transphosphorylation.<br> | |
| − | + | ④ The phosphorylated HssR binds to the direct repeat sequence of the hrtAB promoter and initiates the reporter’s transcription.<br> | |
</font><br> | </font><br> | ||
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<br> | <br> | ||
| − | <h4> Detection System 2: | + | <h4> Detection System 2: HrtR Mechanism </h4> |
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<img src="https://static.igem.org/mediawiki/2017/a/a9/T--KUAS_Korea--HrtR.png"> | <img src="https://static.igem.org/mediawiki/2017/a/a9/T--KUAS_Korea--HrtR.png"> | ||
| − | + | <br><br> | |
| − | <p><font size=4>Also, the mechanism of heme detection using | + | <p><font size=4>Also, the mechanism of heme detection using HrtR protein is as follows.</font></p> |
| − | <p><font size=4> | + | <p><font size=4>① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.<br> |
| − | + | ② When heme binds to the HrtR protein, HrtR binds to the promoter in front of the hrtR sequence and promotes its own transcription.<br> | |
| − | + | ③ The reporter behind the hrtR sequence is transcribed together to determine the presence of heme detection.<br> | |
| − | + | ||
</font><br> | </font><br> | ||
</p> | </p> | ||
Latest revision as of 15:17, 1 November 2017
Design
POO-robiotics
Detection System 1: HssR/S Mechanism
The HssR and HssS mechanism of our project is as follows.
① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.
② When heme in the blood binds to the HssS protein, it phosphorylates histidine 249 through autophosphorylation.
③ HssS transfers the phosphate group from its histidine 249 to aspartate 52 of HssR using transphosphorylation.
④ The phosphorylated HssR binds to the direct repeat sequence of the hrtAB promoter and initiates the reporter’s transcription.
Detection System 2: HrtR Mechanism
Also, the mechanism of heme detection using HrtR protein is as follows.
① If intestinal bleeding occurs for a variety of reasons, blood leak into the bowel.
② When heme binds to the HrtR protein, HrtR binds to the promoter in front of the hrtR sequence and promotes its own transcription.
③ The reporter behind the hrtR sequence is transcribed together to determine the presence of heme detection.
