Zhiling Zhou (Talk | contribs) |
Zhiling Zhou (Talk | contribs) |
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p{ | p{ | ||
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+ | .bs-docs-sidenav | ||
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+ | font-family:Tahoma, Geneva, sans-serif !important; | ||
+ | font-size: 20px !important; | ||
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+ | text-align: left !important; | ||
+ | } | ||
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+ | |||
+ | .textli li{ | ||
+ | font-family:Tahoma, Geneva, sans-serif !important; | ||
+ | font-size: 20px !important; | ||
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} | } | ||
/*文中自定义*/ | /*文中自定义*/ | ||
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<!-- <h1><a class="navbar-brand" href="index.html">MuMei Lab</a></h1> --> | <!-- <h1><a class="navbar-brand" href="index.html">MuMei Lab</a></h1> --> | ||
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− | </ | + | |
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− | + | <a href="https://2017.igem.org/Team:ZJU-China"> | |
− | + | <img style="margin-top:11px" class="navbar-brand" src="https://static.igem.org/mediawiki/2017/d/d5/ZJUChina_logo.png"> | |
− | + | </a> | |
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− | + | <!-- <h1><a class="navbar-brand" href="index.html">My Design</a></h1> --> | |
− | + | </div> | |
− | < | + | <!-- Collect the nav links, forms, and other content for toggling --> |
− | + | <div class="collapse navbar-collapse navbar-ex1-collapse"> | |
− | + | <ul class="nav navbar-nav navbar-right cl-effect-15"> | |
− | + | <!-- Hidden li included to remove active class from about link when scrolled up past about section --> | |
− | + | <li class="hidden"><a class="page-scroll" href="#page-top"></a> </li> | |
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− | + | <li class="m_nav_item dropdown"> | |
+ | <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Overview<b class="caret"></b></a> | ||
+ | <ul class="dropdown-menu "> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Overview">Description</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Demonstrate">Demonstrate</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Applied_Design">Applied Design</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Achievements">Achievements</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Improve">Improve Parts</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/InterLab">InterLab</a></li> | ||
− | + | </ul> | |
− | + | </li> | |
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− | + | <li class="m_nav_item dropdown"> | |
− | + | <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Project<b class="caret"></b></a> | |
− | + | <ul class="dropdown-menu "> | |
− | + | <li><a href="https://2017.igem.org/Team:ZJU-China/Project/tp">Trichoderma Proof</a></li> | |
− | + | <li><a href="https://2017.igem.org/Team:ZJU-China/Project/voc">VOC sensors</a></li> | |
− | + | <li><a style="font-size: 0.7em!important;" href="https://2017.igem.org/Team:ZJU-China/Project/st">Chemical Signal Transduction</a></li> | |
− | + | <li><a style="font-size: 0.7em!important;" href="https://2017.igem.org/Team:ZJU-China/Project/mt">Medium Wave Transduction</a></li> | |
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Project/Downstream">Downstream</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Project/conclusion">Conclusions</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Notebook">Notebook</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Protocols">Protocols</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
− | + | <li class="m_nav_item dropdown" > | |
− | + | <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Model<b class="caret"></b></a> | |
− | + | <ul class="dropdown-menu "> | |
− | + | <!--<li><a href="https://2017.igem.org/Team:ZJU-China/Model">Summery</a></li>--> | |
− | + | <li><a href="https://2017.igem.org/Team:ZJU-China/Model">VOC analysis</a></li> | |
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Model/Coculture">Coculture</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
− | < | + | <li class="m_nav_item dropdown"> |
− | < | + | <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Parts<b class="caret"></b></a> |
− | + | <ul class="dropdown-menu "> | |
− | + | <li><a href="https://2017.igem.org/Team:ZJU-China/Parts">All Parts</a></li> | |
− | < | + | <li><a href="https://2017.igem.org/Team:ZJU-China/Basic_Part">Basic Parts</a></li> |
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Composite_Part">Composite Parts</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Part_Collection">Part Collection</a></li> | ||
− | < | + | </ul> |
+ | </li> | ||
+ | |||
+ | <li class="m_nav_item dropdown" > | ||
+ | <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Hardware<b class="caret"></b></a> | ||
+ | <ul class="dropdown-menu "> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Hardware">Overview</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Hardware/Device">Device</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Hardware/Improvements">Improvements</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Hardware/MediumWave">Medium Wave</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | |||
+ | <li class="m_nav_item dropdown" > | ||
+ | <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Safety<b class="caret"></b></a> | ||
+ | <ul class="dropdown-menu "> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Safety">Environment</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Safety/Lab">Laboratory</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | |||
+ | <li class="m_nav_item dropdown"> | ||
+ | <a href="#" class="dropdown-toggle link" data-toggle="dropdown">HP<b class="caret"></b></a> | ||
+ | <ul class="dropdown-menu "> | ||
+ | |||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Human_Practices">Summary</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/HP/Silver">Silver</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/HP/Gold_Integrated">Gold Integrated</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Engagement">Engagement</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | |||
+ | <li class="m_nav_item dropdown" > | ||
+ | <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Team<b class="caret"></b></a> | ||
+ | <ul class="dropdown-menu "> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Team">Teammates</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Attributions">Attribution</a></li> | ||
+ | <li><a href="https://2017.igem.org/Team:ZJU-China/Collaborations">Collaboration</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </nav> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <p></p> | ||
+ | |||
+ | <div style="width: 100%" class="container zjuContent"> | ||
<div class="col-md-3"></div> | <div class="col-md-3"></div> | ||
<div class="col-md-9" role="main"> | <div class="col-md-9" role="main"> | ||
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<!-- Table --> | <!-- Table --> | ||
− | <table id="partTable" class="table | + | <table id="partTable" class="table"> |
<tr> | <tr> | ||
− | <th>Name</th> | + | <th class="yellowTable">Name</th> |
− | <th>Type</th> | + | <th class="yellowTable">Type</th> |
− | <th>Description</th> | + | <th class="yellowTable">Description</th> |
− | <th>Designer</th> | + | <th class="yellowTable">Designer</th> |
− | <th>Length(bp)</th> | + | <th class="yellowTable">Length(bp)</th> |
− | <th>Submitted</th> | + | <th class="yellowTable">Submitted</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th>BBa_K2207000</th> | + | <th class="grayTable">BBa_K2207000</th> |
<td>Device</td> | <td>Device</td> | ||
<td>H3-RP27 expression system</td> | <td>H3-RP27 expression system</td> | ||
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<td> </td> | <td> </td> | ||
</tr> | </tr> | ||
− | <th>BBa_K2207001</th> | + | <tr> |
+ | <th class="grayTable">BBa_K2207001</th> | ||
<td>Promoters</td> | <td>Promoters</td> | ||
<td>Hex promoter</td> | <td>Hex promoter</td> | ||
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<td>3317</td> | <td>3317</td> | ||
<td></td> | <td></td> | ||
− | + | </tr> | |
<tr> | <tr> | ||
− | <th>BBa_K2207002</th> | + | <th class="grayTable">BBa_K2207002</th> |
<td>Promoters</td> | <td>Promoters</td> | ||
<td>Ech42 promoter</td> | <td>Ech42 promoter</td> | ||
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<tr> | <tr> | ||
− | <th>BBa_K2207003</th> | + | <th class="grayTable">BBa_K2207003</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>Trichoderma HR System I SOD-Tubulin dobule promoter</td> | <td>Trichoderma HR System I SOD-Tubulin dobule promoter</td> | ||
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<tr> | <tr> | ||
− | <th>BBa_K2207004</th> | + | <th class="grayTable">BBa_K2207004</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>Trichoderma HR System II Ech42-H3 double promoter</td> | <td>Trichoderma HR System II Ech42-H3 double promoter</td> | ||
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<tr> | <tr> | ||
− | <th>BBa_K2207005</th> | + | <th class="grayTable">BBa_K2207005</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>Trichoderma HR System III L1-ADH1-RP27-L2</td> | <td>Trichoderma HR System III L1-ADH1-RP27-L2</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th>BBa_K2207006</th> | + | <th class="grayTable">BBa_K2207006</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>Trichoderma HR System IV L3-ADH1-RP27-L4</td> | <td>Trichoderma HR System IV L3-ADH1-RP27-L4</td> | ||
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<tr> | <tr> | ||
− | <th>BBa_K2207007</th> | + | <th class="grayTable">BBa_K2207007</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>Trichoderma HR System V Homologous Binding SiteA</td> | <td>Trichoderma HR System V Homologous Binding SiteA</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th>BBa_K2207008</th> | + | <th class="grayTable">BBa_K2207008</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>Trichoderma HR System VI Homologous Binding SiteB</td> | <td>Trichoderma HR System VI Homologous Binding SiteB</td> | ||
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<tr> | <tr> | ||
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<td>Coding Sequences</td> | <td>Coding Sequences</td> | ||
<td>PhlF transcription factor</td> | <td>PhlF transcription factor</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th>BBa_K2207010</th> | + | <th class="grayTable">BBa_K2207010</th> |
<td>Other</td> | <td>Other</td> | ||
<td>Phl Operator</td> | <td>Phl Operator</td> | ||
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<tr> | <tr> | ||
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<td>Protein Generators</td> | <td>Protein Generators</td> | ||
<td>Phl Operator(BBa_K2207010) mRFP</td> | <td>Phl Operator(BBa_K2207010) mRFP</td> | ||
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<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>DAPG sensor(PhlOF)</td> | <td>DAPG sensor(PhlOF)</td> | ||
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<tr> | <tr> | ||
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<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>2,4-DAPG PhlABCD Cluster</td> | <td>2,4-DAPG PhlABCD Cluster</td> | ||
<td>Junmin Qian</td> | <td>Junmin Qian</td> | ||
<td>4332</td> | <td>4332</td> | ||
− | <td> | + | <td> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th>BBa_K2207014</th> | + | <th class="grayTable">BBa_K2207014</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>PhlAC enzyme</td> | <td>PhlAC enzyme</td> | ||
<td>Junmin Qian</td> | <td>Junmin Qian</td> | ||
<td>5025</td> | <td>5025</td> | ||
− | <td></td> | + | <td> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th>BBa_K2207015</th> | + | <th class="grayTable">BBa_K2207015</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>PhlBD enzyme</td> | <td>PhlBD enzyme</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th>BBa_K2207016</th> | + | <th class="grayTable">BBa_K2207016</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>PhlAB enzyme</td> | <td>PhlAB enzyme</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th>BBa_K2207017</th> | + | <th class="grayTable">BBa_K2207017</th> |
<td>Composite Parts</td> | <td>Composite Parts</td> | ||
<td>PhlCD enzyme</td> | <td>PhlCD enzyme</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th>BBa_K2207018</th> | + | <th class="grayTable">BBa_K2207018</th> |
<td>Devices</td> | <td>Devices</td> | ||
<td>GPD-CYC1 expression system</td> | <td>GPD-CYC1 expression system</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th>BBa_K2207019</th> | + | <th class="grayTable">BBa_K2207019</th> |
<td>Translational units</td> | <td>Translational units</td> | ||
<td>TRPV1-Ferritin</td> | <td>TRPV1-Ferritin</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th>BBa_K2207020</th> | + | <th class="grayTable">BBa_K2207020</th> |
<td>Translational units</td> | <td>Translational units</td> | ||
<td>Ferritin</td> | <td>Ferritin</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th>BBa_K2207021</th> | + | <th class="grayTable">BBa_K2207021</th> |
<td>Promoters</td> | <td>Promoters</td> | ||
<td>Calcineurin-dependent response element (CDRE)</td> | <td>Calcineurin-dependent response element (CDRE)</td> | ||
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<td>✔</td> | <td>✔</td> | ||
</tr> | </tr> | ||
+ | |||
<tr> | <tr> | ||
− | <th>BBa_K2207022</th> | + | <th class="grayTable">BBa_K2207022</th> |
<td>Protein Generators</td> | <td>Protein Generators</td> | ||
− | <td>CDRE(BBa_K2207021) mrfp</td> | + | <td>CDRE(BBa_K2207021) oligoA-mrfp</td> |
<td>Shisheng Li</td> | <td>Shisheng Li</td> | ||
<td>1186</td> | <td>1186</td> | ||
<td>✔</td> | <td>✔</td> | ||
</tr> | </tr> | ||
+ | |||
<tr> | <tr> | ||
− | <th>BBa_K2207023</th> | + | <th class="grayTable">BBa_K2207023</th> |
<td>Coding Sequences</td> | <td>Coding Sequences</td> | ||
<td>Mature serine protein from Paecilomyces lilacinus</td> | <td>Mature serine protein from Paecilomyces lilacinus</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th>BBa_K2207024</th> | + | <th class="grayTable">BBa_K2207024</th> |
<td>Promoters</td> | <td>Promoters</td> | ||
<td>T7-mutant1</td> | <td>T7-mutant1</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th>BBa_K2207025</th> | + | <th class="grayTable">BBa_K2207025</th> |
<td>Promoters</td> | <td>Promoters</td> | ||
<td>T7-mutant2</td> | <td>T7-mutant2</td> | ||
Line 737: | Line 772: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th>BBa_K2207026</th> | + | <th class="grayTable">BBa_K2207026</th> |
<td>Promoters</td> | <td>Promoters</td> | ||
<td>T7-mutant3</td> | <td>T7-mutant3</td> | ||
Line 745: | Line 780: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th>BBa_K2207027</th> | + | <th class="grayTable">BBa_K2207027</th> |
<td>Promoters</td> | <td>Promoters</td> | ||
<td>T7-mutant4</td> | <td>T7-mutant4</td> | ||
Line 753: | Line 788: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th>BBa_K2207028</th> | + | <th class="grayTable">BBa_K2207028</th> |
<td>Promoters</td> | <td>Promoters</td> | ||
<td>T7-mutant5</td> | <td>T7-mutant5</td> | ||
Line 761: | Line 796: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th>BBa_K2207029</th> | + | <th class="grayTable">BBa_K2207029</th> |
<td>Promoters</td> | <td>Promoters</td> | ||
<td>T7-mutant6</td> | <td>T7-mutant6</td> | ||
Line 769: | Line 804: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th>BBa_K2207030</th> | + | <th class="grayTable">BBa_K2207030</th> |
<td>Promoters</td> | <td>Promoters</td> | ||
<td>T7-mutant7</td> | <td>T7-mutant7</td> | ||
Line 777: | Line 812: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <th>BBa_K2207031</th> | + | <th class="grayTable">BBa_K2207031</th> |
<td>Coding Sequences</td> | <td>Coding Sequences</td> | ||
<td>PhlE efflux pump</td> | <td>PhlE efflux pump</td> | ||
− | <td> | + | <td>Junming Qian</td> |
<td>1272</td> | <td>1272</td> | ||
<td>✔</td> | <td>✔</td> | ||
</tr> | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <th class="grayTable">BBa_K2207997</th> | ||
+ | <td>Composite Parts</td> | ||
+ | <td>2,4-DAPG PhlABCD Cluster with T7 promoter and Double Terminator</td> | ||
+ | <td>Junming Qian</td> | ||
+ | <td>1272</td> | ||
+ | <td>✔</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <th class="grayTable">BBa_K2207036</th> | ||
+ | <td>Coding Sequencess</td> | ||
+ | <td>oligoA-mRFP</td> | ||
+ | <td>Shisheng Li</td> | ||
+ | <td>712</td> | ||
+ | <td>✔</td> | ||
+ | </tr> | ||
</table> | </table> | ||
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<h3 class="H3Head">Our new Basic Parts in Trichoderma spp.</h3> | <h3 class="H3Head">Our new Basic Parts in Trichoderma spp.</h3> | ||
<p class="PP Retract"><strong>BBa_K2207003 ~ BBa_K2207008</strong></p> | <p class="PP Retract"><strong>BBa_K2207003 ~ BBa_K2207008</strong></p> | ||
− | + | <p class="PP">We have collected and proved many new parts which can be used in Trichoderma spp. , including four promoters, two terminators and the selection marker hygB gene. And then we cloned them with homologous sequences on pSB1C3,for any researches who want to express genes in Trichoderma spp, they only need to integrate these parts with GOI and clone them into corresponding vectors(no matter what kinds of transduction methods or plasmids he used.) <a class="cite" href="https://2017.igem.org/Team:ZJU-China/Project/tp#overview">Click here</a> to see how we constructed these systems.</p> | |
+ | |||
+ | <h3 class="H3Head">Our calcium ion response promoter.</h3> | ||
+ | <p class="PP Retract"><strong>BBa_K2207021</strong></p> | ||
+ | <p class="PP">We replaced the upstream activating sequence (UAS) of CYC1 promoter with 4 CDREs. This promoter responds to the cell calcium concentration increased, only designed for Saccharomyces cerevisiae and depended on S.cerevisiae's endogenous calcineurin and calmodulin. <a class="cite" href="https://2017.igem.org/Team:ZJU-China/Basic_Part">Click here</a> to see how we use this part.</p> | ||
+ | |||
− | + | <h2 id="BestCompositeParts" class="H2Head">Best Composite Parts</h2> | |
<p class="PP Retract"><strong>BBa_K2207013</strong></p> | <p class="PP Retract"><strong>BBa_K2207013</strong></p> | ||
− | + | <p class="PP">We got a new gene cluster through PCR from Pseudomonadaceae.fluorescens genome,phlABCD,coding four enzymes used to synthesis 2,4-DAPG.We have mutated some illegal enzyme sites and successfully detected the DAPG production.<a class="cite" href="https://2017.igem.org/Team:ZJU-China/Composite_Part">Click here</a> to see our fantastic work.</p> | |
<!--配DAPG基因簇图--> | <!--配DAPG基因簇图--> | ||
<h2 id="ImproveT7promoters" class="H2Head">Improve T7 promoters</h2> | <h2 id="ImproveT7promoters" class="H2Head">Improve T7 promoters</h2> | ||
<p class="PP Retract"><strong>BBa_K2207024 ~ BBa_K2207030</strong></p> | <p class="PP Retract"><strong>BBa_K2207024 ~ BBa_K2207030</strong></p> | ||
− | <p class="PP">T7 promoter is widely used in genetic engineering for its highly yields of recombinant proteins when T7 RNAP is present. This year,we did Site-directed mutagenesis to get some mutants based on T7 promoters.We used mRFP as a reporter gene to measure these mutants strength.Click here to see more details.</p> | + | <p class="PP">T7 promoter is widely used in genetic engineering for its highly yields of recombinant proteins when T7 RNAP is present. This year,we did Site-directed mutagenesis to get some mutants based on T7 promoters.We used mRFP as a reporter gene to measure these mutants strength.<a class="cite" href="https://2017.igem.org/Team:ZJU-China/Part_Collection">Click here</a> to see more details.</p> |
<!--配IP结果处理图--> | <!--配IP结果处理图--> | ||
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<div class="col-md-3 disappear-on-top" role="complementary"> | <div class="col-md-3 disappear-on-top" role="complementary"> | ||
<nav style="position: fixed; top: 100px ; left:50px;" class="bs-docs-sidebar hidden-print hidden-xs hidden-sm"> | <nav style="position: fixed; top: 100px ; left:50px;" class="bs-docs-sidebar hidden-print hidden-xs hidden-sm"> | ||
− | <ul class="nav bs-docs-sidenav"> | + | <ul class="nav bs-docs-sidenav shorterli"> |
<li><a href="#introduction">Introduction</a></li> | <li><a href="#introduction">Introduction</a></li> | ||
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<a class="back-to-top" href="#top"> | <a class="back-to-top" href="#top"> | ||
<!-- <i class="fa fa-arrow-up nav_icon"></i> --> | <!-- <i class="fa fa-arrow-up nav_icon"></i> --> | ||
− | + | <img style="width: 50px;" src="https://static.igem.org/mediawiki/2017/d/da/ZJU_China_UP.png"> | |
</a> | </a> | ||
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<div class="footer" style="background-color: #000000"> | <div class="footer" style="background-color: #000000"> | ||
<div class="copyright container text-center"> | <div class="copyright container text-center"> | ||
+ | <p><br></p> | ||
<p class="copyright">Copyright © 2017 ZJU-IGEM | <a href="http://www.zju.edu.cn/">Zhejiang University</a> |</p> | <p class="copyright">Copyright © 2017 ZJU-IGEM | <a href="http://www.zju.edu.cn/">Zhejiang University</a> |</p> | ||
+ | <p><br></p> | ||
<!--<p class="copyright">Contact us:<a class="copyright" href="">WeChat</a></p>--> | <!--<p class="copyright">Contact us:<a class="copyright" href="">WeChat</a></p>--> | ||
</div> | </div> |
Latest revision as of 15:18, 1 November 2017
Parts
Welcome to the ZJU-China Parts Pages.This year, we designed 31 parts,and 18 of them had been submitted to the iGEM Registry follow the BioBricks assembly standard as described by RFC10.
We have a brief description of our parts here, but if you would like more detailed information on the parts, we strongly recommend you visit the corresponding parts pages on the iGEM BioBricks Registry.
Name | Type | Description | Designer | Length(bp) | Submitted |
---|---|---|---|---|---|
BBa_K2207000 | Device | H3-RP27 expression system | Qianjin Jiang | 2644 | |
BBa_K2207001 | Promoters | Hex promoter | Junbo Yang | 3317 | |
BBa_K2207002 | Promoters | Ech42 promoter | Junbo Yang | 3352 | |
BBa_K2207003 | Composite Parts | Trichoderma HR System I SOD-Tubulin dobule promoter | Yihe Zhang | 3155 | ✔ |
BBa_K2207004 | Composite Parts | Trichoderma HR System II Ech42-H3 double promoter | Yihe Zhang | 4845 | ✔ |
BBa_K2207005 | Composite Parts | Trichoderma HR System III L1-ADH1-RP27-L2 | Yihe Zhang | 903 | ✔ |
BBa_K2207006 | Composite Parts | Trichoderma HR System IV L3-ADH1-RP27-L4 | Yihe Zhang | 903 | ✔ |
BBa_K2207007 | Composite Parts | Trichoderma HR System V Homologous Binding SiteA | Yihe Zhang | 1676 | ✔ |
BBa_K2207008 | Composite Parts | Trichoderma HR System VI Homologous Binding SiteB | Yihe Zhang | 1650 | ✔ |
BBa_K2207009 | Coding Sequences | PhlF transcription factor | Qianjin Jiang | 627 | ✔ |
BBa_K2207010 | Other | Phl Operator | Yuxing Chen | 825 | ✔ |
BBa_K2207011 | Protein Generators | Phl Operator(BBa_K2207010) mRFP | Yuxing Chen | 1545 | |
BBa_K2207012 | Composite Parts | DAPG sensor(PhlOF) | Qianjin Jiang | 1450 | |
BBa_K2207998 | Composite Parts | 2,4-DAPG PhlABCD Cluster | Junmin Qian | 4332 | |
BBa_K2207014 | Composite Parts | PhlAC enzyme | Junmin Qian | 5025 | |
BBa_K2207015 | Composite Parts | PhlBD enzyme | Junmin Qian | 4212 | |
BBa_K2207016 | Composite Parts | PhlAB enzyme | Junmin Qian | 5604 | |
BBa_K2207017 | Composite Parts | PhlCD enzyme | Junmin Qian | 4646 | |
BBa_K2207018 | Devices | GPD-CYC1 expression system | Shisheng Li | 382 | |
BBa_K2207019 | Translational units | TRPV1-Ferritin | Cheng Shen | 4853 | |
BBa_K2207020 | Translational units | Ferritin | Cheng Shen | 1173 | |
BBa_K2207021 | Promoters | Calcineurin-dependent response element (CDRE) | Shisheng Li | 474 | ✔ |
BBa_K2207022 | Protein Generators | CDRE(BBa_K2207021) oligoA-mrfp | Shisheng Li | 1186 | ✔ |
BBa_K2207023 | Coding Sequences | Mature serine protein from Paecilomyces lilacinus | Zifan Xie | 882 | ✔ |
BBa_K2207024 | Promoters | T7-mutant1 | Yuxing Chen | 42 | ✔ |
BBa_K2207025 | Promoters | T7-mutant2 | Yuxing Chen | 42 | ✔ |
BBa_K2207026 | Promoters | T7-mutant3 | Yuxing Chen | 42 | ✔ |
BBa_K2207027 | Promoters | T7-mutant4 | Yuxing Chen | 42 | ✔ |
BBa_K2207028 | Promoters | T7-mutant5 | Yuxing Chen | 42 | ✔ |
BBa_K2207029 | Promoters | T7-mutant6 | Yuxing Chen | 42 | ✔ |
BBa_K2207030 | Promoters | T7-mutant7 | Yuxing Chen | 42 | ✔ |
BBa_K2207031 | Coding Sequences | PhlE efflux pump | Junming Qian | 1272 | ✔ |
BBa_K2207997 | Composite Parts | 2,4-DAPG PhlABCD Cluster with T7 promoter and Double Terminator | Junming Qian | 1272 | ✔ |
BBa_K2207036 | Coding Sequencess | oligoA-mRFP | Shisheng Li | 712 | ✔ |
Favorite Parts!
Our new Basic Parts in Trichoderma spp.
BBa_K2207003 ~ BBa_K2207008
We have collected and proved many new parts which can be used in Trichoderma spp. , including four promoters, two terminators and the selection marker hygB gene. And then we cloned them with homologous sequences on pSB1C3,for any researches who want to express genes in Trichoderma spp, they only need to integrate these parts with GOI and clone them into corresponding vectors(no matter what kinds of transduction methods or plasmids he used.) Click here to see how we constructed these systems.
Our calcium ion response promoter.
BBa_K2207021
We replaced the upstream activating sequence (UAS) of CYC1 promoter with 4 CDREs. This promoter responds to the cell calcium concentration increased, only designed for Saccharomyces cerevisiae and depended on S.cerevisiae's endogenous calcineurin and calmodulin. Click here to see how we use this part.
Best Composite Parts
BBa_K2207013
We got a new gene cluster through PCR from Pseudomonadaceae.fluorescens genome,phlABCD,coding four enzymes used to synthesis 2,4-DAPG.We have mutated some illegal enzyme sites and successfully detected the DAPG production.Click here to see our fantastic work.
Improve T7 promoters
BBa_K2207024 ~ BBa_K2207030
T7 promoter is widely used in genetic engineering for its highly yields of recombinant proteins when T7 RNAP is present. This year,we did Site-directed mutagenesis to get some mutants based on T7 promoters.We used mRFP as a reporter gene to measure these mutants strength.Click here to see more details.