Difference between revisions of "Team:ZJU-China/Parts"

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{{ZJU-China}}
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<html lang="zh-CN">
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<html>
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<h1>Parts</h1>
 
  
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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/* set page to fill window*/
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h5>Note</h5>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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  width:100%;
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<div class="column half_size">
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}
  
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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<p>The information needed to initially create a part on the Registry is:</p>
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<ul>
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<li>Part Name</li>
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}
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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</ul>
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<p>
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.TeamImage {
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h5>Inspiration</h5>
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<p>We have a created a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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                  <!-- <h1><a class="navbar-brand" href="index.html">MuMei Lab</a></h1> -->
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 +
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                      <li class="m_nav_item dropdown">
 +
                          <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Overview<b class="caret"></b></a>
 +
                          <ul class="dropdown-menu ">
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Overview">Description</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Demonstrate">Demonstrate</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Applied_Design">Applied Design</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Achievements">Achievements</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Improve">Improve Parts</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/InterLab">InterLab</a></li>
 +
 
 +
                          </ul>
 +
                      </li>
 +
 
 +
                      <li class="m_nav_item dropdown">
 +
                          <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Project<b class="caret"></b></a>
 +
                          <ul class="dropdown-menu ">
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Project/tp">Trichoderma Proof</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Project/voc">VOC sensors</a></li>
 +
                              <li><a style="font-size: 0.7em!important;" href="https://2017.igem.org/Team:ZJU-China/Project/st">Chemical Signal Transduction</a></li>
 +
                              <li><a style="font-size: 0.7em!important;" href="https://2017.igem.org/Team:ZJU-China/Project/mt">Medium Wave Transduction</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Project/Downstream">Downstream</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Project/conclusion">Conclusions</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Notebook">Notebook</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Protocols">Protocols</a></li>
 +
                          </ul>
 +
                      </li>
 +
 
 +
                      <li class="m_nav_item dropdown" >
 +
                          <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Model<b class="caret"></b></a>
 +
                          <ul class="dropdown-menu ">
 +
                              <!--<li><a href="https://2017.igem.org/Team:ZJU-China/Model">Summery</a></li>-->
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Model">VOC analysis</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Model/Coculture">Coculture</a></li>
 +
 
 +
                          </ul>
 +
                      </li>
 +
 
 +
                      <li class="m_nav_item dropdown">
 +
                          <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Parts<b class="caret"></b></a>
 +
                          <ul class="dropdown-menu ">
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Parts">All Parts</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Basic_Part">Basic Parts</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Composite_Part">Composite Parts</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Part_Collection">Part Collection</a></li>
 +
 
 +
 
 +
 
 +
 
 +
                          </ul>
 +
                      </li>
 +
 
 +
                      <li class="m_nav_item dropdown" >
 +
                          <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Hardware<b class="caret"></b></a>
 +
                          <ul class="dropdown-menu ">
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Hardware">Overview</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Hardware/Device">Device</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Hardware/Improvements">Improvements</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Hardware/MediumWave">Medium Wave</a></li>
 +
                          </ul>
 +
                      </li>
 +
 
 +
                      <li class="m_nav_item dropdown" >
 +
                          <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Safety<b class="caret"></b></a>
 +
                          <ul class="dropdown-menu ">
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Safety">Environment</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Safety/Lab">Laboratory</a></li>
 +
                          </ul>
 +
                      </li>
 +
 
 +
                      <li class="m_nav_item dropdown">
 +
                          <a href="#" class="dropdown-toggle link" data-toggle="dropdown">HP<b class="caret"></b></a>
 +
                          <ul class="dropdown-menu ">
 +
 
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Human_Practices">Summary</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/HP/Silver">Silver</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/HP/Gold_Integrated">Gold Integrated</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Engagement">Engagement</a></li>
 +
                          </ul>
 +
                      </li>
 +
 
 +
                      <li class="m_nav_item dropdown" >
 +
                          <a href="#" class="dropdown-toggle link" data-toggle="dropdown">Team<b class="caret"></b></a>
 +
                          <ul class="dropdown-menu ">
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Team">Teammates</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Attributions">Attribution</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:ZJU-China/Collaborations">Collaboration</a></li>
 +
                          </ul>
 +
                      </li>
 +
                  </ul>
 +
              </div>
 +
          </nav>
 +
      </div>
 +
  </div>
 +
 
 +
  <p></p>
 +
 
 +
<div style="width: 100%" class="container zjuContent">
 +
  <div class="col-md-3"></div>
 +
    <div class="col-md-9" role="main">
 +
      <div class="bs-docs-section">
 +
 
 +
          <div style="height: 50px;"></div>
 +
  <h1 id="introduction" class="page-header ArticleHead GreenAH">Parts</h1>
 +
  <p  class="PP">Welcome to the ZJU-China Parts Pages.This year, we designed 31 parts,and 18 of them had been submitted to the iGEM Registry follow the BioBricks assembly standard as described by RFC10.</p>
 +
  <p class="PP">We have a brief description of our parts here, but if you would like more detailed information on the parts, we strongly recommend you visit the corresponding parts pages on the iGEM BioBricks Registry.</p>
 +
 
 +
  <!-- Table -->
 +
 
 +
  <table id="partTable" class="table">
 +
  <tr>
 +
<th class="yellowTable">Name</th>
 +
<th class="yellowTable">Type</th>
 +
<th class="yellowTable">Description</th>
 +
<th class="yellowTable">Designer</th>
 +
<th class="yellowTable">Length(bp)</th>
 +
<th class="yellowTable">Submitted</th>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
    <th class="grayTable">BBa_K2207000</th>
 +
    <td>Device</td>
 +
    <td>H3-RP27 expression system</td>
 +
    <td>Qianjin Jiang</td>
 +
    <td>2644</td>
 +
    <td> </td>
 +
</tr>
 +
      <tr>
 +
      <th class="grayTable">BBa_K2207001</th>
 +
      <td>Promoters</td>
 +
      <td>Hex promoter</td>
 +
      <td>Junbo Yang</td>
 +
      <td>3317</td>
 +
      <td></td>
 +
      </tr>
 +
<tr>
 +
    <th class="grayTable">BBa_K2207002</th>
 +
    <td>Promoters</td>
 +
    <td>Ech42 promoter</td>
 +
    <td>Junbo Yang</td>
 +
    <td>3352</td>
 +
    <td></td>
 +
</tr>
 +
 
 +
<tr>
 +
    <th class="grayTable">BBa_K2207003</th>
 +
    <td>Composite Parts</td>
 +
    <td>Trichoderma HR System I SOD-Tubulin dobule promoter</td>
 +
    <td>Yihe Zhang</td>
 +
    <td>3155</td>
 +
    <td>✔</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
    <th class="grayTable">BBa_K2207004</th>
 +
    <td>Composite Parts</td>
 +
    <td>Trichoderma HR System II Ech42-H3 double promoter</td>
 +
    <td>Yihe Zhang</td>
 +
    <td>4845</td>
 +
    <td>✔</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
    <th class="grayTable">BBa_K2207005</th>
 +
    <td>Composite Parts</td>
 +
    <td>Trichoderma HR System III L1-ADH1-RP27-L2</td>
 +
    <td>Yihe Zhang</td>
 +
    <td>903</td>
 +
    <td>✔</td>
 +
 
 +
</tr>
 +
<tr>
 +
    <th class="grayTable">BBa_K2207006</th>
 +
    <td>Composite Parts</td>
 +
    <td>Trichoderma HR System IV L3-ADH1-RP27-L4</td>
 +
    <td>Yihe Zhang</td>
 +
    <td>903</td>
 +
    <td>✔</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
    <th class="grayTable">BBa_K2207007</th>
 +
    <td>Composite Parts</td>
 +
    <td>Trichoderma HR System V Homologous Binding SiteA</td>
 +
    <td>Yihe Zhang</td>
 +
    <td>1676</td>
 +
    <td>✔</td>
 +
 
 +
</tr>
 +
<tr>
 +
    <th class="grayTable">BBa_K2207008</th>
 +
    <td>Composite Parts</td>
 +
    <td>Trichoderma HR System VI Homologous Binding SiteB</td>
 +
    <td>Yihe Zhang</td>
 +
    <td>1650</td>
 +
    <td>✔</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
    <th class="grayTable">BBa_K2207009</th>
 +
    <td>Coding Sequences</td>
 +
    <td>PhlF transcription factor</td>
 +
    <td>Qianjin Jiang</td>
 +
    <td>627</td>
 +
    <td>✔</td>
 +
 
 +
</tr>
 +
<tr>
 +
    <th class="grayTable">BBa_K2207010</th>
 +
    <td>Other</td>
 +
    <td>Phl Operator</td>
 +
    <td>Yuxing Chen</td>
 +
    <td>825</td>
 +
    <td>✔</td>
 +
 
 +
</tr>
 +
 
 +
<tr>
 +
    <th class="grayTable">BBa_K2207011</th>
 +
    <td>Protein Generators</td>
 +
    <td>Phl Operator(BBa_K2207010) mRFP</td>
 +
    <td>Yuxing Chen</td>
 +
    <td>1545</td>
 +
    <td></td>
 +
 
 +
</tr>
 +
<tr>
 +
    <th class="grayTable">BBa_K2207012</th>
 +
    <td>Composite Parts</td>
 +
    <td>DAPG sensor(PhlOF)</td>
 +
    <td>Qianjin Jiang</td>
 +
    <td>1450</td>
 +
    <td></td>
 +
</tr>
 +
 
 +
<tr>
 +
    <th class="grayTable">BBa_K2207998</th>
 +
    <td>Composite Parts</td>
 +
    <td>2,4-DAPG PhlABCD Cluster</td>
 +
    <td>Junmin Qian</td>
 +
    <td>4332</td>
 +
    <td> </td>
 +
</tr>
 +
 
 +
<tr>
 +
    <th class="grayTable">BBa_K2207014</th>
 +
    <td>Composite Parts</td>
 +
    <td>PhlAC enzyme</td>
 +
    <td>Junmin Qian</td>
 +
    <td>5025</td>
 +
    <td> </td>
 +
</tr>
 +
 
 +
<tr>
 +
    <th class="grayTable">BBa_K2207015</th>
 +
    <td>Composite Parts</td>
 +
    <td>PhlBD enzyme</td>
 +
    <td>Junmin Qian</td>
 +
    <td>4212</td>
 +
    <td></td>
 +
</tr>
 +
<tr>
 +
    <th class="grayTable">BBa_K2207016</th>
 +
    <td>Composite Parts</td>
 +
    <td>PhlAB enzyme</td>
 +
    <td>Junmin Qian</td>
 +
    <td>5604</td>
 +
    <td></td>
 +
</tr>
 +
<tr>
 +
    <th class="grayTable">BBa_K2207017</th>
 +
    <td>Composite Parts</td>
 +
    <td>PhlCD enzyme</td>
 +
    <td>Junmin Qian</td>
 +
    <td>4646</td>
 +
    <td></td>
 +
</tr>
 +
<tr>
 +
    <th class="grayTable">BBa_K2207018</th>
 +
    <td>Devices</td>
 +
    <td>GPD-CYC1 expression system</td>
 +
    <td>Shisheng Li</td>
 +
    <td>382</td>
 +
    <td></td>
 +
</tr>
 +
<tr>
 +
    <th class="grayTable">BBa_K2207019</th>
 +
    <td>Translational units</td>
 +
    <td>TRPV1-Ferritin</td>
 +
    <td>Cheng Shen</td>
 +
    <td>4853</td>
 +
    <td></td>
 +
</tr>
 +
<tr>
 +
    <th class="grayTable">BBa_K2207020</th>
 +
    <td>Translational units</td>
 +
    <td>Ferritin</td>
 +
    <td>Cheng Shen</td>
 +
    <td>1173</td>
 +
    <td></td>
 +
 
 +
</tr>
 +
<tr>
 +
    <th class="grayTable">BBa_K2207021</th>
 +
    <td>Promoters</td>
 +
    <td>Calcineurin-dependent response element (CDRE)</td>
 +
    <td>Shisheng Li</td>
 +
    <td>474</td>
 +
    <td>✔</td>
 +
</tr>
 +
 
 +
<tr>
 +
    <th class="grayTable">BBa_K2207022</th>
 +
    <td>Protein Generators</td>
 +
    <td>CDRE(BBa_K2207021) oligoA-mrfp</td>
 +
    <td>Shisheng Li</td>
 +
    <td>1186</td>
 +
    <td>✔</td>
 +
</tr>
 +
 
 +
<tr>
 +
    <th class="grayTable">BBa_K2207023</th>
 +
    <td>Coding Sequences</td>
 +
    <td>Mature serine protein from Paecilomyces lilacinus</td>
 +
    <td>Zifan Xie</td>
 +
    <td>882</td>
 +
    <td>✔</td>
 +
</tr>
 +
<tr>
 +
    <th class="grayTable">BBa_K2207024</th>
 +
    <td>Promoters</td>
 +
    <td>T7-mutant1</td>
 +
    <td>Yuxing Chen</td>
 +
    <td>42</td>
 +
    <td>✔</td>
 +
 
 +
</tr>
 +
<tr>
 +
    <th class="grayTable">BBa_K2207025</th>
 +
    <td>Promoters</td>
 +
    <td>T7-mutant2</td>
 +
    <td>Yuxing Chen</td>
 +
    <td>42</td>
 +
    <td>✔</td>
 +
</tr>
 +
<tr>
 +
    <th class="grayTable">BBa_K2207026</th>
 +
    <td>Promoters</td>
 +
    <td>T7-mutant3</td>
 +
    <td>Yuxing Chen</td>
 +
    <td>42</td>
 +
    <td>✔</td>
 +
</tr>
 +
<tr>
 +
    <th class="grayTable">BBa_K2207027</th>
 +
    <td>Promoters</td>
 +
    <td>T7-mutant4</td>
 +
    <td>Yuxing Chen</td>
 +
    <td>42</td>
 +
    <td>✔</td>
 +
</tr>
 +
<tr>
 +
    <th class="grayTable">BBa_K2207028</th>
 +
    <td>Promoters</td>
 +
    <td>T7-mutant5</td>
 +
    <td>Yuxing Chen</td>
 +
    <td>42</td>
 +
    <td>✔</td>
 +
</tr>
 +
<tr>
 +
    <th class="grayTable">BBa_K2207029</th>
 +
    <td>Promoters</td>
 +
    <td>T7-mutant6</td>
 +
    <td>Yuxing Chen</td>
 +
    <td>42</td>
 +
    <td>✔</td>
 +
</tr>
 +
<tr>
 +
    <th class="grayTable">BBa_K2207030</th>
 +
    <td>Promoters</td>
 +
    <td>T7-mutant7</td>
 +
    <td>Yuxing Chen</td>
 +
    <td>42</td>
 +
    <td>✔</td>
 +
</tr>
 +
  <tr>
 +
      <th class="grayTable">BBa_K2207031</th>
 +
      <td>Coding Sequences</td>
 +
      <td>PhlE efflux pump</td>
 +
      <td>Junming Qian</td>
 +
      <td>1272</td>
 +
      <td>✔</td>
 +
  </tr>
 +
 
 +
      <tr>
 +
          <th class="grayTable">BBa_K2207997</th>
 +
          <td>Composite Parts</td>
 +
          <td>2,4-DAPG PhlABCD Cluster with T7 promoter and Double Terminator</td>
 +
          <td>Junming Qian</td>
 +
          <td>1272</td>
 +
          <td>✔</td>
 +
      </tr>
 +
 
 +
      <tr>
 +
          <th class="grayTable">BBa_K2207036</th>
 +
          <td>Coding Sequencess</td>
 +
          <td>oligoA-mRFP</td>
 +
          <td>Shisheng Li</td>
 +
          <td>712</td>
 +
          <td>✔</td>
 +
      </tr>
 +
 
 +
  </table>
 +
 
 +
  <h2 id="favouriteParts" class="H2Head">Favorite Parts!</h2>
 +
          <h3 class="H3Head">Our new Basic Parts in Trichoderma spp.</h3>
 +
          <p class="PP Retract"><strong>BBa_K2207003 ~ BBa_K2207008</strong></p>
 +
          <p class="PP">We have collected and proved many new parts which can be used in Trichoderma spp. , including four promoters, two terminators and the selection marker hygB gene. And then we cloned them with homologous sequences on pSB1C3,for any researches who want to express genes in Trichoderma spp, they only need to integrate these parts with GOI and clone them into corresponding vectors(no matter what kinds of transduction methods or plasmids he used.) <a class="cite" href="https://2017.igem.org/Team:ZJU-China/Project/tp#overview">Click here</a> to see how we constructed these systems.</p>
 +
 
 +
          <h3 class="H3Head">Our calcium ion response  promoter.</h3>
 +
              <p class="PP Retract"><strong>BBa_K2207021</strong></p>
 +
          <p class="PP">We replaced the upstream activating sequence (UAS) of CYC1 promoter with 4 CDREs. This promoter responds to the cell calcium concentration increased, only designed for Saccharomyces cerevisiae and depended on S.cerevisiae's endogenous calcineurin and calmodulin. <a class="cite" href="https://2017.igem.org/Team:ZJU-China/Basic_Part">Click here</a> to see how we use this part.</p>
 +
 
 +
 
 +
          <h2 id="BestCompositeParts" class="H2Head">Best Composite Parts</h2>
 +
          <p class="PP Retract"><strong>BBa_K2207013</strong></p>
 +
          <p class="PP">We got a new gene cluster through PCR from Pseudomonadaceae.fluorescens genome,phlABCD,coding four enzymes used to synthesis 2,4-DAPG.We have mutated some illegal enzyme sites and successfully detected the DAPG production.<a class="cite" href="https://2017.igem.org/Team:ZJU-China/Composite_Part">Click here</a> to see our fantastic work.</p>
 +
  <!--配DAPG基因簇图-->
 +
 
 +
  <h2 id="ImproveT7promoters" class="H2Head">Improve T7 promoters</h2>
 +
          <p class="PP Retract"><strong>BBa_K2207024 ~ BBa_K2207030</strong></p>
 +
          <p class="PP">T7 promoter is widely used in genetic engineering for its highly yields of recombinant proteins when T7 RNAP is present. This year,we did Site-directed mutagenesis to get some mutants based on T7 promoters.We used mRFP as a reporter gene to measure these mutants strength.<a class="cite" href="https://2017.igem.org/Team:ZJU-China/Part_Collection">Click here</a> to see more details.</p>
 +
      <!--配IP结果处理图-->
  
−
<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
−
<ul>
 
−
<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
−
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
−
</ul>
 
 
</div>
 
</div>
 +
       
  
−
<div class="column full_size">
 
−
<h5>Part Table </h5>
 
  
−
<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
+
<!-- 右侧监听开始 -->
 +
<div class="col-md-3 disappear-on-top" role="complementary">
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  <nav  style="position: fixed; top: 100px ; left:50px;" class="bs-docs-sidebar hidden-print hidden-xs hidden-sm">
 +
    <ul class="nav bs-docs-sidenav shorterli">
  
−
<div class="highlight">
+
      <li><a href="#introduction">Introduction</a></li>
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Latest revision as of 15:18, 1 November 2017

Parts

Welcome to the ZJU-China Parts Pages.This year, we designed 31 parts,and 18 of them had been submitted to the iGEM Registry follow the BioBricks assembly standard as described by RFC10.

We have a brief description of our parts here, but if you would like more detailed information on the parts, we strongly recommend you visit the corresponding parts pages on the iGEM BioBricks Registry.

Name Type Description Designer Length(bp) Submitted
BBa_K2207000 Device H3-RP27 expression system Qianjin Jiang 2644
BBa_K2207001 Promoters Hex promoter Junbo Yang 3317
BBa_K2207002 Promoters Ech42 promoter Junbo Yang 3352
BBa_K2207003 Composite Parts Trichoderma HR System I SOD-Tubulin dobule promoter Yihe Zhang 3155 ✔
BBa_K2207004 Composite Parts Trichoderma HR System II Ech42-H3 double promoter Yihe Zhang 4845 ✔
BBa_K2207005 Composite Parts Trichoderma HR System III L1-ADH1-RP27-L2 Yihe Zhang 903 ✔
BBa_K2207006 Composite Parts Trichoderma HR System IV L3-ADH1-RP27-L4 Yihe Zhang 903 ✔
BBa_K2207007 Composite Parts Trichoderma HR System V Homologous Binding SiteA Yihe Zhang 1676 ✔
BBa_K2207008 Composite Parts Trichoderma HR System VI Homologous Binding SiteB Yihe Zhang 1650 ✔
BBa_K2207009 Coding Sequences PhlF transcription factor Qianjin Jiang 627 ✔
BBa_K2207010 Other Phl Operator Yuxing Chen 825 ✔
BBa_K2207011 Protein Generators Phl Operator(BBa_K2207010) mRFP Yuxing Chen 1545
BBa_K2207012 Composite Parts DAPG sensor(PhlOF) Qianjin Jiang 1450
BBa_K2207998 Composite Parts 2,4-DAPG PhlABCD Cluster Junmin Qian 4332
BBa_K2207014 Composite Parts PhlAC enzyme Junmin Qian 5025
BBa_K2207015 Composite Parts PhlBD enzyme Junmin Qian 4212
BBa_K2207016 Composite Parts PhlAB enzyme Junmin Qian 5604
BBa_K2207017 Composite Parts PhlCD enzyme Junmin Qian 4646
BBa_K2207018 Devices GPD-CYC1 expression system Shisheng Li 382
BBa_K2207019 Translational units TRPV1-Ferritin Cheng Shen 4853
BBa_K2207020 Translational units Ferritin Cheng Shen 1173
BBa_K2207021 Promoters Calcineurin-dependent response element (CDRE) Shisheng Li 474 ✔
BBa_K2207022 Protein Generators CDRE(BBa_K2207021) oligoA-mrfp Shisheng Li 1186 ✔
BBa_K2207023 Coding Sequences Mature serine protein from Paecilomyces lilacinus Zifan Xie 882 ✔
BBa_K2207024 Promoters T7-mutant1 Yuxing Chen 42 ✔
BBa_K2207025 Promoters T7-mutant2 Yuxing Chen 42 ✔
BBa_K2207026 Promoters T7-mutant3 Yuxing Chen 42 ✔
BBa_K2207027 Promoters T7-mutant4 Yuxing Chen 42 ✔
BBa_K2207028 Promoters T7-mutant5 Yuxing Chen 42 ✔
BBa_K2207029 Promoters T7-mutant6 Yuxing Chen 42 ✔
BBa_K2207030 Promoters T7-mutant7 Yuxing Chen 42 ✔
BBa_K2207031 Coding Sequences PhlE efflux pump Junming Qian 1272 ✔
BBa_K2207997 Composite Parts 2,4-DAPG PhlABCD Cluster with T7 promoter and Double Terminator Junming Qian 1272 ✔
BBa_K2207036 Coding Sequencess oligoA-mRFP Shisheng Li 712 ✔

Favorite Parts!

Our new Basic Parts in Trichoderma spp.

BBa_K2207003 ~ BBa_K2207008

We have collected and proved many new parts which can be used in Trichoderma spp. , including four promoters, two terminators and the selection marker hygB gene. And then we cloned them with homologous sequences on pSB1C3,for any researches who want to express genes in Trichoderma spp, they only need to integrate these parts with GOI and clone them into corresponding vectors(no matter what kinds of transduction methods or plasmids he used.) Click here to see how we constructed these systems.

Our calcium ion response promoter.

BBa_K2207021

We replaced the upstream activating sequence (UAS) of CYC1 promoter with 4 CDREs. This promoter responds to the cell calcium concentration increased, only designed for Saccharomyces cerevisiae and depended on S.cerevisiae's endogenous calcineurin and calmodulin. Click here to see how we use this part.

Best Composite Parts

BBa_K2207013

We got a new gene cluster through PCR from Pseudomonadaceae.fluorescens genome,phlABCD,coding four enzymes used to synthesis 2,4-DAPG.We have mutated some illegal enzyme sites and successfully detected the DAPG production.Click here to see our fantastic work.

Improve T7 promoters

BBa_K2207024 ~ BBa_K2207030

T7 promoter is widely used in genetic engineering for its highly yields of recombinant proteins when T7 RNAP is present. This year,we did Site-directed mutagenesis to get some mutants based on T7 promoters.We used mRFP as a reporter gene to measure these mutants strength.Click here to see more details.


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