Difference between revisions of "Team:DEIAGRA/InterLab"

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color:black;mso-bidi-font-weight:normal'>2. Fluorescence Standard Curves<o:p></o:p></span></h2>
 
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Revision as of 15:49, 1 November 2017

Fourth International Inter-Lab Measurement Study

 

 

This year Inter-lab Measurement Study aims to establish a GFP measurement protocol based on engineering principles for testing six RBS devices (BCDs) that are intended to make gene expression more precise and reliable  in labs around the world.

Team DEIAGRA had followed the plate reader protocol for this study as mentioned on (https://static.igem.org/mediawiki/2017/8/85/InterLab_2017_Plate_Reader_Protocol.pdf) for obtaining the data represented below in form of graphs.

 

 

 

1.                   OD​600​ Reference point

 

 

 

LUDOX

H2O

Replicate 1

0.0667

0.0473

Replicate 2

0.0617

0.0489

Replicate 3

0.0628

0.0515

Replicate 4

0.0668

0.0506

Arith. Mean

0.0645

0.049575

Corrected Abs600

0.014925

Reference OD600

0.0425

OD600/Abs600

2.8475712

 

 

 

 

 

 

 

 

 

 

 

2. Fluorescence Standard Curves

(uM Fluorescein)

 

 

 

3.Fluorescence Curve

FluoroscenceCurveReading (Colony 1)

 

 

4.Absorbance Curve

(Optical Density 600 nM)