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<div> | <div> | ||
<h4><strong>1.1 SDS-PAGE verification for <I>OprF-3*LBT 4</I> & <I>OprF-Sitag</I> expression</strong> </h4> | <h4><strong>1.1 SDS-PAGE verification for <I>OprF-3*LBT 4</I> & <I>OprF-Sitag</I> expression</strong> </h4> | ||
+ | <p>Whether the OpRF-3*LBT or OpRF-Sitag can be expressed or not needs to be detected by SDS-PAGE. | ||
<img title="demo1" src="https://static.igem.org/mediawiki/2017/b/b7/2017_HUST_China_demostrate002.png" class="col-xs-6 col-xs-offset-3" style="padding: 10px 0px;"> | <img title="demo1" src="https://static.igem.org/mediawiki/2017/b/b7/2017_HUST_China_demostrate002.png" class="col-xs-6 col-xs-offset-3" style="padding: 10px 0px;"> | ||
<img title="demo1" src="https://static.igem.org/mediawiki/2017/c/ce/2017_HUST_China_demostrate003.png" class="col-xs-6 col-xs-offset-3" style="padding: 10px 0px;"> | <img title="demo1" src="https://static.igem.org/mediawiki/2017/c/ce/2017_HUST_China_demostrate003.png" class="col-xs-6 col-xs-offset-3" style="padding: 10px 0px;"> | ||
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<div class="col-xs-12"> | <div class="col-xs-12"> | ||
<h4><strong>1.2 Verification of Cell Surface Display System by Immunofluorescent assay</strong> </h4> | <h4><strong>1.2 Verification of Cell Surface Display System by Immunofluorescent assay</strong> </h4> | ||
+ | <p>Then we did immunofluorescence assay to figure out whether the cell surface display system works or not.</p> | ||
<img title="demo1" src="https://static.igem.org/mediawiki/2017/c/cc/2017_HUST_China_demostrate004.png" class="col-xs-8 col-xs-offset-2" style="padding: 10px 0px;"> | <img title="demo1" src="https://static.igem.org/mediawiki/2017/c/cc/2017_HUST_China_demostrate004.png" class="col-xs-8 col-xs-offset-2" style="padding: 10px 0px;"> | ||
<p class="col-xs-12">Figure 3 shows the result of our verification of our cell surface display system. Some cells can show a considerable fluorescent intensity, while some perform partial or weak fluorescence which can not be detected by our microscope camera. But this result still successfully demonstrated the cell surface display of our LBTs. </p> | <p class="col-xs-12">Figure 3 shows the result of our verification of our cell surface display system. Some cells can show a considerable fluorescent intensity, while some perform partial or weak fluorescence which can not be detected by our microscope camera. But this result still successfully demonstrated the cell surface display of our LBTs. </p> |
Revision as of 16:03, 1 November 2017