Difference between revisions of "Team:Cadets2Vets/Description"

 
(54 intermediate revisions by 5 users not shown)
Line 1: Line 1:
−
{{Cadets2Vets}}
+
{{Team:Cadets2Vets/SideMenu}}
 +
 
 +
 
 
<html>
 
<html>
−
<body class="header-page  wsite-theme-light  wsite-page-index"><div class="wrapper">
+
<head>
−
  <div class="header-wrap">
+
<title>
−
    <div id="topBar" class="topbar">
+
Description - Home</title>
−
      <label class="hamburger"><span></span></label>
+
<!-- META -->
−
      <div class="logo"><span class="wsite-logo">
+
<meta charset="UTF-8">
 +
<meta name="viewport" content="width=device-width, maximum-scale=1"/>  
 +
  
 +
<!-- CSS -->
 +
<link href="https://2017.igem.org/Team:Cadets2Vets/css/common?action=raw&ctype=text/css" rel="stylesheet">
 +
                <link href="https://2017.igem.org/Team:Cadets2Vets/css/all_css?action=raw&ctype=text/css" rel="stylesheet">
 
 
−
<span class="wsite-title-placeholder">&nbsp;</span><span style="display:none">
 
−
<span style="display:none">Acquire</span>
 
−
</span>
 
 
 
 +
 +
<link rel="icon" type="image/png" href="https://static.igem.org/mediawiki/2017/7/71/Circleimage1.png" />
 +
<link rel="apple-touch-icon" href="https://static.igem.org/mediawiki/2017/7/71/Circleimage1.png" />
 +
 +
 +
 +
<link id="vbid-59a5b-fnxcttvm-STRIPE_DATA" link href="https://2017.igem.org/Team:Cadets2Vets/css/stripe_data_css?action=raw&ctype=text/css" rel="stylesheet">
 +
 +
<link id="vbid-04d15a56-3o81aqko-STRIPE_DATA" link href="https://2017.igem.org/Team:Cadets2Vets/css/description/stripe_data_css?action=raw&ctype=text/css" rel="stylesheet">
 +
 +
<link href="https://2017.igem.org/Team:Cadets2Vets/css/fonts?action=raw&ctype=text/css" rel="stylesheet">
 +
                <link href="https://2017.igem.org/Team:Cadets2Vets/css/effects?action=raw&ctype=text/css" rel="stylesheet">
 +
                <link href="https://2017.igem.org/Team:Cadets2Vets/css/lightbox?action=raw&ctype=text/css" rel="stylesheet">
 +
                <link href="https://2017.igem.org/Team:Cadets2Vets/css/spimeview?action=raw&ctype=text/css" rel="stylesheet">
 +
 +
 +
<link id="vbid-04d15a56-3o81aqko-STATIC_STYLE" link href="https://2017.igem.org/Team:Cadets2Vets/css/description/static_style?action=raw&ctype=text/css" rel="stylesheet">
 +
 +
<!-- SCRIPT -->
 +
<script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/xprs_helper?action=raw&ctype=text/javascript"></script>
  
−
</span></div>
+
<script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/all_js?action=raw&ctype=text/javascript"></script>
−
         
+
<script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/lib/touchswipe/jquery_mobile_custom_min?action=raw&ctype=text/javascript"></script>
−
         
+
−
    </div><!-- end .container -->
+
 +
 +
 +
<!-- User analytics -->
  
−
  <div class="banner-wrap">
+
 
−
<div class="wsite-elements wsite-not-footer wsite-header-elements">
+
<!-- Serving analytics -->
−
<div class="wsite-section-wrap">
+
<script>
−
<div  class="wsite-section wsite-header-section wsite-section-bg-image" style="height: 804px;vertical-align: bottom;background-image: url(https://static.igem.org/mediawiki/2017/thumb/3/34/Pic_pic_aai.jpg/450px-Pic_pic_aai.jpg) ;background-repeat: no-repeat ;background-position: 50.00% 65.91% ;background-size: 100% ;background-color: transparent ;background-size: cover;" >
+
var currentDomain =  window.location.host;
−
<div class="wsite-section-content">
+
if (currentDomain != "localhost:7000"){
 +
var _gaq = _gaq || [];
 +
_gaq.push(['_setAccount', 'UA-58695071-1']);
 +
_gaq.push(['_setDomainName', currentDomain]);
 +
_gaq.push(['_setAllowLinker', true]);
 +
_gaq.push(['_trackPageview']);
 +
}
 +
</script>
 +
 +
<!-- Label config-->
 +
<script>
 +
 +
var LABEL_CONFIG = {};
 +
 +
</script></head>
 +
 
 +
<body class="fast-animated-bg fixed-bg " data-ecommerce-solution="NONE" data-root-id="vbid-59a5b-fnxcttvm" data-root-style-id="style-59a5b-yamofliu" data-default-currency="USD" data-osid="osid--6af0e67f-af8681d1"  data-app-version="1.4.1f" data-caller="live"  data-ecommerce-dashboard="https://dashboard.shoprocket.co" data-static-server="//www.imcreator.com" data-imos-server="https://imos004-dot-im--os.appspot.com">
 +
 +
<div id="xprs" data-website-name="Home" class="xprs-holder" >
 +
<div class="main-page "  >
 +
<div id="content">
 +
<div  id="vbid-04d15a56-3o81aqko"  class="master container style-04d15a56-okeof5tv content stripes  website-style " data-itemtype="folder" data-creator="" data-itemname="Description"  data-itemslug="description" data-itemstyleid="style-04d15a56-okeof5tv" data-margintop="" data-arranger="stripes" data-layout="middle" data-vbid="vbid-04d15a56-3o81aqko" data-preset-type-id="" data-preview-style="style-04d15a56-okeof5tv" data-style="style-7f715-gkcpu3e69m" data-absolute-path=""  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="stripes"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="false"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT=""
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="middle"></div>
 +
<div id="children">
 +
 +
 +
 +
<!-- MENUS START -->
 +
<div  id="vbid-59a5b-1xbnq2ek" class="master item-box  header-box style-59a5b-jbqq3wgo injected        " data-holder-type="header"" data-holder-type="header"  data-child-type="ORIGIN"  data-styleid="style-59a5b-jbqq3wgo" data-preview-styleid='style-59a5b-jbqq3wgo' data-preset-type-id="MENUS">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="header-wrapper item-wrapper menus-wrapper ">
 +
 +
<div class="item-content leaf menu_layout header content" data-preview-style="style-59a5b-jbqq3wgo" data-style="style-7f715-scjjarqgxu"  data-behavior-type="NOTHING" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-59a5b-1xbnq2ek" data-bgimg="">
 +
<div class="preview-content-wrapper">
 +
<div class="preview-content-holder">
 +
<div class="left-div">
 +
<div class="benet" style="min-height:inherit;"></div>
 +
<div class="logo-holder">
 +
<!-- ICON TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 
 +
</div>
 +
<div class="helper-div">
 +
<div class="item-details menu">
 +
<!-- TITLE TEMPLATE -->
 
 
−
<div class="container">
+
<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
−
<div class="banner">
+
 
−
<div class="wsite-section-elements">
+
<!-- SUBTITLE TEMPLATE -->
−
<h2 class="wsite-content-title"><font color="#d5d5d5">Our Project</font><br /></h2>
+
−
</div>
+
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
−
</div>
+
 
−
</div>
+
</div>
−
+
 
</div>
 
</div>
−
<div class=""></div>
 
 
</div>
 
</div>
−
</div>
+
<div class="right-div">
 +
<div class="benet" style="min-height:inherit;"></div>
 +
<!-- LINKS TEMPLATE -->
 +
<div class="preview-item-links ">
 +
<ul class="preview-links-wrapper">
  
−
</div>
 
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="index.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-a1a56421418f7ce"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Home</span>
 +
</a>
 +
 +
</li>
  
−
</div>
+
−
  </div><!-- end .header-wrap -->
+
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="collaborations.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-5efad87d43e4352"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Collaborations</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="description.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-e6e2afb357139e4"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Description</span>
 +
</a>
 +
 +
</li>
  
−
<div class="main-wrap">
+
−
<div id="wsite-content" class="wsite-elements wsite-not-footer">
+
−
<div class="wsite-section-wrap">
+
<li class="removable-parent">
−
<div class="wsite-section wsite-body-section wsite-background-1 wsite-custom-background" >
+
−
<div class="wsite-section-content">
+
<a class="removable-parent" href="design.html" data-link-type="EXISTING"  target="_self" >
−
    <div class="container">
+
−
<div class="wsite-section-elements">
+
<span id="element-c6287513fca8901" class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Design</span>
−
<div class="wsite-spacer" style="height:50px;"></div>
+
</a>
 +
 +
</li>
  
−
<h2 class="wsite-content-title" style="text-align:center;"><font size="5" color="#2a2a2a"><font>Project Description</font></font></h2>
+
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="experiments.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-f4b485a3e8b3825" class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Experiments</span>
 +
</a>
 +
 +
</li>
  
−
<div class="paragraph" style="text-align:center;"><font size="2">The goal of this project is to engineer a biological circuit utilizing multiple e.coli DNA regulatory sequences and a green fluorescent protein (GFP) reporter as a means to detect Arsenic (See following list of parts). Once this construct is designed and constructed the goal will be to demonstrate this circuit as viable in a cell free lateral flow paper based system for the detection of &nbsp;Arsenic. Through this proof of concept we will be working closely with local Nonprofits Urban WThe goal of this project is to engineer a biological circuit utilizing multiple e.coli DNA regulatory sequences and a green fluorescent protein (GFP) reporter as a means to detect Arsenic (See list of parts in parts section). Once this construct is designed and constructed the goal will be to demonstrate this circuit as viable in a cell arsenic. Through this proof of concept we will be working closely with local Nonprofits Urban Waters and Readiness Acceleration and Innovation Network (RAIN) as well as a local high school Charles Wright Academy. Through this heave community involvement we hope to both increase pre-collegiate interest in STEM related fields, increase the communal understanding of the local environment and provide a cheap and effective tool for the detection of potentially toxic metals (Arsenic) and Readiness Acceleration and Innovation Network (RAIN) as well as a local high school Charles Wright Academy. Through this heave community involvement we hope to both increase pre-collegiate interest in STEM related fields, increase the communal understanding of the local environment and provide a cheap and effective tool for the detection of potentially toxic metals (Arsenic).<br /></font><br /><span></span><br /></div>
+
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="notebook.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-603d2303312c4bd"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook</span>
 +
</a>
 +
 +
</li>
  
−
<div><div class="wsite-multicol"><div class="wsite-multicol-table-wrap" style="margin:0 -15px;">
+
−
<table class="wsite-multicol-table">
+
−
<tbody class="wsite-multicol-tbody">
+
<li class="removable-parent">
−
<tr class="wsite-multicol-tr">
+
−
<td class="wsite-multicol-col" style="width:50%; padding:0 15px;">
+
<a class="removable-parent" href="notebook-august.html" data-link-type="EXISTING"  target="_self" >
−
+
−
+
<span id="element-e39fe0beffa33cd" class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook August</span>
 +
</a>
 +
 +
</li>
  
−
<div class="paragraph" style="text-align:center;">What is Synthetic Biology?<ul><li>Synthetic Biology is the shift from Understanding and modifying biology to treating biology as an engineering discipline where basic building blocks are assembled from the ground up into larger engineered systems<br /></li></ul><br /></div>
+
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="notebook-july.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-09ca318ecaf1b2f"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook July</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="notebook-june.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-367041e6445b247"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook June</span>
 +
</a>
 +
 +
</li>
  
−
+
−
</td> <td class="wsite-multicol-col" style="width:50%; padding:0 15px;">
+
−
+
<li class="removable-parent">
−
+
 +
<a class="removable-parent" href="public-engagement.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-307cb61a9a844e1"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Public Engagement</span>
 +
</a>
 +
 +
</li>
  
−
<div><div class="wsite-image wsite-image-border-none " style="padding-top:10px;padding-bottom:0px;margin-left:0px;margin-right:0px;text-align:left">
+
−
<a>
+
−
<img src="/uploads/1/0/7/7/107726947/published/cell.jpeg?1497288862" alt="Picture" style="width:auto;max-width:100%" />
+
<li class="removable-parent">
−
</a>
+
−
<div style="display:block;font-size:90%"></div>
+
<a class="removable-parent" href="safety.html" data-link-type="EXISTING"   target="_self" >
−
</div></div>
+
 +
<span id="element-fa38138e1dd4fce"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Safety</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="team.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-8308751370782bc"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Team</span>
 +
</a>
 +
 +
</li>
  
−
+
−
</td> </tr>
+
−
</tbody>
+
<li class="removable-parent">
−
</table>
+
−
</div></div></div>
+
<a class="removable-parent" href="basic-parts.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-78fbd0423085ce2"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Basic Parts</span>
 +
</a>
 +
 +
</li>
  
−
<div><div class="wsite-multicol"><div class="wsite-multicol-table-wrap" style="margin:0 -5px;">
+
−
<table class="wsite-multicol-table">
+
−
<tbody class="wsite-multicol-tbody">
+
<li class="removable-parent">
−
<tr class="wsite-multicol-tr">
+
−
<td class="wsite-multicol-col" style="width:50%; padding:0 5px;">
+
<a class="removable-parent" href="composite-parts.html" data-link-type="EXISTING"  target="_self" >
−
+
−
+
<span id="element-24ea54f35798e9a" class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Composite Parts</span>
 +
</a>
 +
 +
</li>
  
−
<div class="paragraph">Goals of Synthetic biology<ul><li>Develop tools to work at increasing levels of abstraction, and to enable facile manufacturing of designs</li><li>Leverage expertise from established engineering disciplines</li><li>Ultimately, forward engineer novel solutions to diverse problems</li></ul></div>
+
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="parts.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-8d68d4dbac38a10"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Parts</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="interlab.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-c92600692c09125"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Interlab</span>
 +
</a>
 +
 +
</li>
  
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="our-partners.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-be35f8069be1925"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Our Partners</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="notebook-september.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-4d7dd9f5c90ef9b"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook September</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="environmental-project.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="2d522622"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Environmental Project</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="hardware.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="8d3cfa8c"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Hardware</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="demonstrate.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="1ad7c153"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Demonstrate</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="improve.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="d9b23d12"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Improve</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="results.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="7e0ed437"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Results</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="attribution.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="8a69633b"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Attribution</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="notebook-october.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="643d5085"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook October</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="silver-hp.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-563894f97a8ebaf"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Silver HP</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="integrated-and-gold.html" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="element-874635e92416b44"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Integrated and Gold</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</ul>
 +
</div>
 +
 +
 +
<button class="hamburger links-menu-btn  small" type="button">
 +
  <span class="hamburger-box">
 +
  <span class="hamburger-inner"></span>
 +
  </span>
 +
</button>
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="menu"
 +
data-MENU_OVERLAY="relative"
 +
data-MENU_SCROLL="false"
 +
data-ALWAYS_MINIFIED="false"
 +
data-MENU_POSITION="none"
 +
data-MENU_ALIGN="left"
 +
data-BACKGROUND_COLOR="rgb(124, 100, 41)"
 +
></div>
 +
</div>
 
 
−
</td> <td class="wsite-multicol-col" style="width:50%; padding:0 5px;">
+
</div>
 +
</div>
 +
<!-- MENUS END -->
 +
 +
 +
 +
 +
<!-- PROMO START -->
 +
<div  id="vbid-04d15a56-prooe7yt"  class="master item-box  gallery-box style-04d15a56-7epp0bbe          " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-04d15a56-7epp0bbe" data-preview-styleid='style-04d15a56-7epp0bbe' data-preset-type-id="PROMO">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper promo-wrapper ">
 
 
−
+
<div  class="sub container style-04d15a56-7epp0bbe content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-04d15a56-7epp0bbe" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-04d15a56-prooe7yt" data-preset-type-id="PROMO" data-preview-style="style-04d15a56-7epp0bbe" data-style="style-f3095-j0xhjgzynh" data-absolute-path=""  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-04d15a56-rgcbfzyd"  class="sub item-box  page-box style-04d15a56-7epp0bbe          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-04d15a56-7epp0bbe" data-preview-styleid='style-04d15a56-7epp0bbe' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-04d15a56-rgcbfzyd" data-preview-style="style-04d15a56-7epp0bbe" data-style="style-f3095-yq39oaomol" data-orig-thumb-height="1073" data-orig-thumb-width="2048" data-vbid="vbid-04d15a56-rgcbfzyd" data-bgimg="https://static.igem.org/mediawiki/2017/c/c3/C2V_Description_Background.jpeg">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-04d15a56-4md5tn4s-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
<div><div class="wsite-image wsite-image-border-none " style="padding-top:10px;padding-bottom:10px;margin-left:0;margin-right:0;text-align:right">
+
<div id="vbid-04d15a56-4md5tn4s" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://static.igem.org/mediawiki/2017/c/c3/C2V_Description_Background.jpeg);"  data-orig-width="2048" data-orig-height="1073" >
−
<a>
+
<!-- VIDEO TEMPLATE -->
−
<img src="/uploads/1/0/7/7/107726947/arsnic-redesing-1-1_orig.png" alt="Picture" style="width:auto;max-width:100%" />
+
−
</a>
+
−
<div style="display:block;font-size:90%"></div>
+
−
</div></div>
+
  
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
  
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h1 id="vbid-04d15a56-x26nl7vy" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >DESCRIPTION OF OUR PROJECT</h1>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-04d15a56-zlmqmubp" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p><span id="docs-internal-guid-1b7da29c-fd54-c3a1-2826-3c55b3c31a0c"><span style="font-size: 12pt; color: rgb(0, 0, 0); background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">The goal of this project is to engineer a biological circuit that utilizes </span><span style="font-size: 12pt; color: rgb(0, 0, 0); background-color: transparent; font-style: italic; vertical-align: baseline; white-space: pre-wrap;">E. coli</span><span style="font-size: 12pt; color: rgb(0, 0, 0); background-color: transparent; vertical-align: baseline; white-space: pre-wrap;"> plasmid DNA sequences and a green fluorescent protein (GFP) reporter to detect arsenic. The constitutive promoter in our construct produces a regulatory protein ArsR that binds with arsenic ions and triggers visible fluorescence. Our team will demonstrate this circuit using a cell-free lateral flow paper-based system. Ultimately, the purpose of this circuit will be to provide an inexpensive and ubiquitous arsenic sensor for testing environmental samples. </span></span>​</p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 
 
−
</td> </tr>
 
−
</tbody>
 
−
</table>
 
−
</div></div></div>
 
 
</div>
 
</div>
 
</div>
 
</div>
 +
 +
 +
 +
 +
 +
</div>
 
</div>
 
</div>
−
 
+
 +
 +
 
</div>
 
</div>
 
</div>
 
</div>
−
<div class="wsite-section-wrap">
+
−
<div class="wsite-section wsite-body-section wsite-section-bg-color wsite-background-2 wsite-custom-background" style="background-color: #f8f8f8;background-image: none;is_customized: 1;" >
+
−
<div class="wsite-section-content">
+
−
    <div class="container">
+
−
<div class="wsite-section-elements">
+
−
+
 
</div>
 
</div>
 
</div>
 
</div>
−
</div>
+
<!-- PROMO END -->
 +
 +
 +
 +
 +
<!-- PRICING START -->
 +
<div  id="vbid-6c9d2635-sv3yd6h4"  class="master item-box  gallery-box style-6c9d2635-eb0lt7ih    items-hover-effects item_hover2 items-enter-effects item_enter2    " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="NATIVE_ORDER"  data-styleid="style-6c9d2635-eb0lt7ih" data-preview-styleid='style-6c9d2635-eb0lt7ih' data-preset-type-id="PRICING">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper pricing-wrapper ">
 +
 +
<div  class="sub container style-6c9d2635-eb0lt7ih content matrix  " data-itemtype="folder" data-creator="" data-itemname="LOADING"  data-itemslug="loading" data-itemstyleid="style-6c9d2635-eb0lt7ih" data-margintop="" data-arranger="matrix" data-layout="multi" data-vbid="vbid-6c9d2635-sv3yd6h4" data-preset-type-id="PRICING" data-preview-style="style-6c9d2635-eb0lt7ih" data-style="style-f2783-7himpqznhz" data-absolute-path=""  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="matrix"
 +
data-ARRANGER_COLS="3"
 +
data-ARRANGER_ITEM_MAX_WIDTH="1000"
 +
data-ARRANGER_ITEM_MIN_WIDTH="228.89999999999998"
 +
data-ARRANGER_ITEM_RATIO="0.8"
 +
data-ARRANGER_ITEM_SPACING="4"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="false"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT=""
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-6c9d2635-ybzm333p"  class="sub item-box  page-box style-6c9d2635-eb0lt7ih          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-6c9d2635-eb0lt7ih" data-preview-styleid='style-6c9d2635-eb0lt7ih' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-6c9d2635-ybzm333p" data-preview-style="style-6c9d2635-eb0lt7ih" data-style="style-f2783-oogpxtqvka" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-6c9d2635-ybzm333p" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 
 +
<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 
 +
 
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
  
 
</div>
 
</div>
 +
 
</div>
 
</div>
−
<div class="wsite-section-wrap">
+
−
<div class="wsite-section wsite-body-section wsite-background-3 wsite-custom-background" >
+
 
−
<div class="wsite-section-content">
+
</div>
−
    <div class="container">
+
</div>
−
<div class="wsite-section-elements">
+
<div class="text-side shrinker-parent">
−
+
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-6c9d2635-qbra6yji" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Goal #1<br></h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 
 +
 +
 +
 
 +
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 +
 
 +
 
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-6c9d2635-pvckqd8j" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p><span style="font-size:20px;"><span id="docs-internal-guid-1b7da29c-09db-8e3c-11bc-a8b64c92574a"><span style="font-family: Arial; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">Design and create a synthetic biological circuit containing a reporter protein regulated by arsenic ions.</span></span></span></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 
 +
 +
 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 
 +
 +
<div class="preview-form order-handle">
 +
 
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 +
</div>
 +
 +
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
 +
 
 +
 
 +
 +
 +
<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
 +
 
 +
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 
</div>
 
</div>
 
</div>
 
</div>
−
</div>
 
−
 
 
</div>
 
</div>
 
</div>
 
</div>
−
<div class="wsite-section-wrap">
+
</div>
−
<div class="wsite-section wsite-body-section wsite-background-4 wsite-custom-background"  >
+
<!-- LAYOUT SETTINGS -->
−
<div class="wsite-section-content">
+
<div class="layout-settings" style="display:none;" data-type="multi"></div>
−
    <div class="container">
+
−
<div class="wsite-section-elements">
+
−
+
 
</div>
 
</div>
 
</div>
 
</div>
−
</div>
+
 +
 +
 +
 +
 +
 +
<div  id="vbid-6c9d2635-k0xsms7i"  class="sub item-box  page-box style-6c9d2635-eb0lt7ih          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-6c9d2635-eb0lt7ih" data-preview-styleid='style-6c9d2635-eb0lt7ih' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-6c9d2635-k0xsms7i" data-preview-style="style-6c9d2635-eb0lt7ih" data-style="style-f2783-oogpxtqvka" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-6c9d2635-k0xsms7i" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 
 +
<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 
 +
 
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
  
 
</div>
 
</div>
 +
 
</div>
 
</div>
 +
  
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-6c9d2635-ul5d7ano" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Goal #2<br></h2>
 +
 
</div>
 
</div>
 +
<br class="lower-line-break" />
  
−
  </div>
+
 +
  
−
    <div class="footer-wrap">
+
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
−
    <div class="container">
+
 
−
    <div class="footer"><div id="weebly-footer-signup-container">
+
 
−
<div class="signup-container-header trigger-signup">
+
−
<button class="start-free">Create a free website</button>
+
−
<div class="powered-by">
+
<br class="upper-line-break" />
−
Powered by <span class="link weebly-icon"></span>
+
<div class="preview-body-holder removable-parent order-handle">
−
</div>
+
 +
<div id="vbid-6c9d2635-0atjkqde" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p><span style="font-size:20px;"><span id="docs-internal-guid-1b7da29c-09dc-2147-201d-0fee97b1058e"><span style="font-family: Arial; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">Demonstrate that the circuit can detect arsenic in solution in cells or using cell-free lysates.</span></span></span></p>
 
</div>
 
</div>
 +
 
</div>
 
</div>
 +
<br class="lower-line-break" />
  
−
<script type="text/javascript" src="//cdn2.editmysite.com/js/site/footerSignup.js?buildTime=1497287536"></script>
+
−
<script type="text/javascript">
+
−
Weebly.footer.setupContainer('cdn2.editmysite.com', '1497287536', 'www.weebly.com');
+
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
−
</script>
+
 
 +
 +
<div class="preview-form order-handle">
 +
 
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 
</div>
 
</div>
−
    </div><!-- end container -->
+
−
    </div><!-- end footer-wrap -->
+
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 
</div>
 
</div>
 +
</div>
 +
 +
  
−
<div class="w-navpane nav mobile-nav">
+
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
−
<label class="hamburger w-navpane-trigger"><span></span></label>
+
 
−
<ul class="wsite-menu-default">
+
 
−
<li id="active" class="wsite-menu-item-wrap">
+
−
<a
+
−
href="/"
+
<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
−
class="wsite-menu-item"
+
 
−
>
+
−
Home
+
−
</a>
+
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 
 
−
</li>
+
</div>
−
<li id="pg222259470211333982" class="wsite-menu-item-wrap">
+
</div>
−
<a
+
−
href="/services.html"
+
−
class="wsite-menu-item"
+
−
>
+
−
Services
+
−
</a>
+
 +
<div  id="vbid-6c9d2635-rzieffte" class="sub item-box  page-box style-6c9d2635-eb0lt7ih          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-6c9d2635-eb0lt7ih" data-preview-styleid='style-6c9d2635-eb0lt7ih' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 
 
−
</li>
+
<div class="item-content leaf multi_layout page content -container" data-self="vbid-6c9d2635-rzieffte" data-preview-style="style-6c9d2635-eb0lt7ih" data-style="style-f2783-oogpxtqvka" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-6c9d2635-rzieffte" data-bgimg="">
−
<li id="pg176222549264264975" class="wsite-menu-item-wrap">
+
<div  class="multi-container preview image-cover" >
−
<a
+
<div class="Picture item-preview">
−
href="/about.html"
+
<div class="preview-image-holder">
−
class="wsite-menu-item"
+
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
−
>
+
</div>
−
About
+
<div class="helper-div middle-center" >
−
</a>
+
<!-- <div class="benet" style="min-height:inherit;"></div> -->
−
+
<div class="pic-side">
−
</li>
+
<div class="vertical-aligner">
−
<li id="pg736091085660923040" class="wsite-menu-item-wrap">
+
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
−
<a
+
−
href="/news.html"
+
−
class="wsite-menu-item"
+
−
>
+
−
News
+
−
</a>
+
−
+
−
</li>
+
−
<li id="pg581528279135988304" class="wsite-menu-item-wrap">
+
−
<a
+
−
href="/contact.html"
+
−
class="wsite-menu-item"
+
−
>
+
−
Contact
+
−
</a>
+
−
+
−
</li>
+
−
</ul>
+
−
</div>
+
  
−
<script type="text/javascript" src="/files/theme/plugins.js?1494340460"></script>
+
<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
−
<script type="text/javascript" src="/files/theme/custom.js?1494340460"></script>
+
<!-- VIDEO TEMPLATE -->
 +
  
−
<script type="text/javascript">
+
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
  
−
var _gaq = _gaq || [];
 
−
_gaq.push(['_setAccount', 'UA-7870337-1']);
 
−
_gaq.push(['_setDomainName', 'none']);
 
−
_gaq.push(['_setAllowLinker', true]);
 
−
_gaq.push(['_trackPageview']);
 
  
−
(function() {
+
<!-- MAP TEMPLATE -->
−
var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true;
+
−
ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js';
+
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
−
var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s);
+
−
})();
+
  
−
_W.Analytics = _W.Analytics || {'trackers': {}};
+
<!-- RAW TEMPLATE -->
−
_W.Analytics.trackers.wGA = '_gaq';
+
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
  
−
</script>
+
</div>
 +
 +
</div>
 +
  
−
<script type="text/javascript" async=1>
+
</div>
−
;(function(p,l,o,w,i,n,g){if(!p[i]){p.GlobalSnowplowNamespace=p.GlobalSnowplowNamespace||[];
+
</div>
−
p.GlobalSnowplowNamespace.push(i);p[i]=function(){(p[i].q=p[i].q||[]).push(arguments)
+
<div class="text-side shrinker-parent">
−
};p[i].q=p[i].q||[];n=l.createElement(o);g=l.getElementsByTagName(o)[0];n.async=1;
+
<div class="vertical-aligner">
−
n.src=w;g.parentNode.insertBefore(n,g)}}(window,document,'script','//cdn2.editmysite.com/js/wsnbn/snowday262.js','snowday'));
+
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
  
−
var r = [99, 104, 101, 99, 107, 111, 117, 116, 46, 40, 119, 101, 101, 98, 108, 121, 124, 101, 100, 105, 116, 109, 121, 115, 105, 116, 101, 41, 46, 99, 111, 109];
+
−
var snPlObR = function(arr) {
+
−
var s = '';
+
<br class="upper-line-break" />
−
for (var i = 0 ; i < arr.length ; i++){
+
<div class="preview-title-holder removable-parent order-handle">
−
s = s + String.fromCharCode(arr[i]);
+
−
}
+
<h2 id="vbid-6c9d2635-lns7saw8" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Goal #3<br></h2>
−
return s;
+
−
};
+
</div>
−
var s = snPlObR(r);
+
<br class="lower-line-break" />
  
−
var regEx = new RegExp(s);
+
 +
  
−
_W.Analytics = _W.Analytics || {'trackers': {}};
+
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
−
_W.Analytics.trackers.wSP = 'snowday';
+
−
_W.Analytics.user_id = '107726947';
+
−
_W.Analytics.site_id = '775468353378266231';
+
  
  
−
(function(app_id, ec_hostname, discover_root_domain) {
+
−
var track = window[_W.Analytics.trackers.wSP];
+
−
if (!track) return;
+
<br class="upper-line-break" />
−
track('newTracker', app_id, ec_hostname, {
+
<div class="preview-body-holder removable-parent order-handle">
−
appId: app_id,
+
−
post: true,
+
<div id="vbid-6c9d2635-yxv565h7" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
−
platform: 'web',
+
<p><span style="font-size:20px;"><span id="docs-internal-guid-1b7da29c-09dd-066b-7acc-ba488d576ad6"><span style="font-family: Arial; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">Determine the conditions needed for cell-free lysates to detect EPA limits for harmful concentrations of arsenic.</span></span></span></p>
−
discoverRootDomain: discover_root_domain,
+
</div>
−
cookieName: '_snow_',
+
−
contexts: {
+
</div>
−
webPage: true,
+
<br class="lower-line-break" />
−
performanceTiming: true,
+
−
gaCookies: true
+
−
},
+
−
crossDomainLinker: function (linkElement) {
+
−
return regEx.test(linkElement.href);
+
−
}
+
−
});
+
−
track('trackPageView', _W.Analytics.user_id+':'+_W.Analytics.site_id);
+
−
track('crossDomainLinker', function (linkElement) {
+
−
return regEx.test(linkElement.href);
+
−
});
+
−
})(
+
−
'_wn',
+
−
'ec.editmysite.com',
+
−
false
+
−
);
+
−
</script>
+
  
−
<!-- Quantcast Tag -->
+
−
<script type="text/javascript">
+
−
var _qevents = _qevents || [];
+
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
  
−
(function() {
+
−
var elem = document.createElement('script');
+
<div class="preview-form order-handle">
−
elem.src = "https://secure.quantserve.com/quant.js";
+
−
elem.async = true;
+
−
elem.type = "text/javascript";
+
−
var scpt = document.getElementsByTagName('script')[0];
+
−
scpt.parentNode.insertBefore(elem, scpt);
+
−
})();
+
  
−
_qevents.push({
+
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
−
qacct:"p-0dYLvhSGGqUWo",
+
</div>
−
labels:"l0,u107726947.u107726947s775468353378266231"
+
−
});
+
<div class="preview-item-links order-handle ">
−
</script>
+
<div class="preview-links-wrapper">
  
−
<noscript>
+
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
−
<div style="display:none;">
+
−
<img src="//pixel.quantserve.com/pixel/p-0dYLvhSGGqUWo.gif?labels=l0,u107726947.u107726947s775468353378266231" border="0" height="1" width="1" alt="Quantcast"/>
+
</div>
 
</div>
 
</div>
−
</noscript>
+
−
<!-- End Quantcast tag -->
+
  
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
  
−
<script>
 
  
−
(function(jQuery) {
+
−
try {
+
−
if (jQuery) {
+
<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
−
jQuery('div.blog-social div.fb-like').attr('class', 'blog-social-item blog-fb-like');
+
−
var $commentFrame = jQuery('#commentArea iframe');
+
−
if ($commentFrame.length > 0) {
+
−
var frameHeight = jQuery($commentFrame[0].contentWindow.document).height() + 50;
+
−
$commentFrame.css('min-height', frameHeight + 'px');
+
−
}
+
−
if (jQuery('.product-button').length > 0){
+
−
jQuery(document).ready(function(){
+
−
jQuery('.product-button').parent().each(function(index, product){
+
−
if(jQuery(product).attr('target') == 'paypal'){
+
−
if (!jQuery(product).find('> [name="bn"]').length){
+
−
jQuery('<input>').attr({
+
−
type: 'hidden',
+
−
name: 'bn',
+
−
value: 'DragAndDropBuil_SP_EC'
+
−
}).appendTo(product);
+
−
}
+
−
}
+
−
});
+
−
});
+
−
}
+
−
}
+
−
else {
+
−
// Prototype
+
−
$$('div.blog-social div.fb-like').each(function(div) {
+
−
div.className = 'blog-social-item blog-fb-like';
+
−
});
+
−
$$('#commentArea iframe').each(function(iframe) {
+
−
iframe.style.minHeight = '410px';
+
−
});
+
−
}
+
−
}
+
−
catch(ex) {}
+
−
})(window._W && _W.jQuery);
+
  
−
</script>
+
−
<div id="eu-cookie" class="notification" style="display: none;">
+
−
<span id="eu-cookie-content"></span>
+
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
−
<span id="eu-cookie-close">&#10005;</span>
+
 
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 
</div>
 
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- PRICING END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-4c38363c-pwfs3s8z"  class="master item-box  gallery-box style-4c38363c-cfykn4og    button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-4c38363c-cfykn4og" data-preview-styleid='style-4c38363c-cfykn4og' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-4c38363c-cfykn4og content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-4c38363c-cfykn4og" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-4c38363c-pwfs3s8z" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-4c38363c-cfykn4og" data-style="style-4e411-j0xhjgzynh" data-absolute-path=""  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-4c38363c-4gaxjzpo"  class="sub item-box  page-box style-4c38363c-cfykn4og          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-4c38363c-cfykn4og" data-preview-styleid='style-4c38363c-cfykn4og' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-4c38363c-4gaxjzpo" data-preview-style="style-4c38363c-cfykn4og" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="640" data-orig-thumb-width="505" data-vbid="vbid-4c38363c-4gaxjzpo" data-bgimg="https://static.igem.org/mediawiki/2017/f/f2/C2V_Description_WhyWeChoseArs.jpg">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-right" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-4c38363c-jm6adqzn-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
<script type="text/javascript">
+
<div  id="vbid-4c38363c-jm6adqzn" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://static.igem.org/mediawiki/2017/f/f2/C2V_Description_WhyWeChoseArs.jpg);"  data-orig-width="505" data-orig-height="640" >
−
(function($) {
+
<!-- VIDEO TEMPLATE -->
−
window.cookie_location = '';
+
−
// this cookie_content should already be pre-encoded from CookieSettingView.js
+
−
window.cookie_content = '' || _W.stl('templates.publish.virtual-includes.footerCode_1');
+
−
window.cookie_policy_key = '775468353378266231_cookie_policy';
+
−
window.cookie_content_hash = '';
+
  
−
if (!_W.getCookie(window.cookie_policy_key) || _W.getCookie(window.cookie_policy_key) !== window.cookie_content_hash){
+
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
−
if (window.cookie_location === 'top' || window.cookie_location === 'bottom'){
+
−
$('#eu-cookie-content').text(decodeURIComponent(window.cookie_content));
+
−
(window.cookie_location === 'top') ? animateFromTop() : animateFromBottom();
+
−
$('#eu-cookie').show();
+
−
}
+
−
}
+
  
−
$('#eu-cookie-close').click(function(){
 
−
_W.setCookie(window.cookie_policy_key, window.cookie_content_hash, 100000);
 
−
$('#eu-cookie').hide();
 
−
});
 
  
−
function animateFromTop(){
+
<!-- MAP TEMPLATE -->
−
$('#eu-cookie').css({
+
−
'top': '0px',
+
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
−
'animation': 'reveal-top 1 2s'
+
−
});
+
−
}
+
  
−
function animateFromBottom(){
+
<!-- RAW TEMPLATE -->
−
$('#eu-cookie').css({
+
−
'bottom': '0px',
+
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
−
'animation': 'reveal-bottom 1 2s'
+
−
});
+
−
}
+
−
})(window._W && _W.jQuery);
+
−
</script>
+
  
−
</body>
+
</div>
 +
 +
</div>
 +
  
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-4c38363c-lhrwnuia" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >why we chose arsenic<br></h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
  
−
<div class="column full_size">
+
−
<h2>Project Description</h2>
+
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-divider-holder removable-parent order-handle">
 +
<div id="vbid-4c38363c-i5dg9lve" class="preview-element preview-divider magic-circle-holder quick-text-style-menu" data-menu-name="PREVIEW_DIVIDER">
 +
</div>
 +
</div>
 +
<br class="lower-line-break" />
  
−
<p>The goal of this project is to engineer a biological circuit utilizing multiple e.coli DNA regulatory sequences and a green fluorescent protein (GFP) reporter as a means to detect Arsenic (See following list of parts). Once this construct is designed and constructed the goal will be to demonstrate this circuit as viable in a cell free lateral flow paper based system for the detection of  Arsenic. Through this proof of concept we will be working closely with local Nonprofits Urban WThe goal of this project is to engineer a biological circuit utilizing multiple e.coli DNA regulatory sequences and a green fluorescent protein (GFP) reporter as a means to detect Arsenic (See list of parts in parts section). Once this construct is designed and constructed the goal will be to demonstrate this circuit as viable in a cell arsenic. Through this proof of concept we will be working closely with local Nonprofits Urban Waters and Readiness Acceleration and Innovation Network (RAIN) as well as a local high school Charles Wright Academy. Through this heave community involvement we hope to both increase pre-collegiate interest in STEM related fields, increase the communal understanding of the local environment and provide a cheap and effective tool for the detection of potentially toxic metals (Arsenic) and Readiness Acceleration and Innovation Network (RAIN) as well as a local high school Charles Wright Academy. Through this heave community involvement we hope to both increase pre-collegiate interest in STEM related fields, increase the communal understanding of the local environment and provide a cheap and effective tool for the detection of potentially toxic metals (Arsenic).
+
−
</p>
+
−
<h4> What is Synthetic Biology? <h4/>
+
−
<li>Synthetic Biology is the shift from Understanding and modifying biology to treating biology as an engineering discipline where basic building  blocks are assembled from the ground up into larger engineered systems<li/>
+
−
<div/>
+
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-4c38363c-ccbscqzl" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p><span style="line-height:1em;"><span style="font-size:16px;"><span style="letter-spacing: 1.1px; white-space: pre-wrap;">Arsenic was selected by Cadets2Vets as the first chemical we would attempt to detect using a biological circuit. It is naturally found in the environment, especially in groundwater, and around industrial plants as a byproduct of manufacturing practices.</span>​</span></span></p><p><br></p><p><span style="line-height:1em;"><span style="font-size:16px;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">The detection of arsenic is especially important to team </span></span><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">members in the Puget Sound area, because North Tacoma is a Superfund Site. The ASARCO smelting company smokestack, erected in 1917, was a symbol of economic prosperity and booming business in the developing city of Tacoma. The copper smelting site on Ruston’s shore in Puget Sound brought jobs and wealth to the area. Unfortunately, over the course of its 100 years of operation, the plant had been pumping toxic materials like arsenic and lead directly into the air, where it then settled into the surrounding soil and water of the greater Tacoma area.</span></span></span></span></p><p><br></p><p><span style="line-height:1em;"><span style="font-size:16px;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">The smokestack was demolished in 1985, but there are still toxic levels of heavy metals in the soil over 30 years later. The city of Tacoma takes measures to warn new Tacoma homeowners about how to mitigate any increased levels of exposure to toxic materials in their soil, but must also take more drastic steps to protect users of publicly-owned land.</span><br class="kix-line-break"><br class="kix-line-break"><span style="vertical-align: baseline; white-space: pre-wrap;">The Department of Defense has a strong interest in developing rapid assays to test for environmental risks to protect the health of service members. Troops often deploy to hostile environments and assessing the risks of the environment itself can be challenging. The development of the arsenic circuit represents a new way to use synthetic biology in the military setting. Demonstrating that a biological circuit can be used in this application will lead to an expansion of the technology to test for more substances in the future. </span></span></span></span></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
  
−
<div class="column full_size">
+
−
<h4> Goals of Synthetic Biology <h4/>
+
−
<div/>
+
−
<li> Develop tools to work at increasing levels of abstraction, and to enable facile manufacturing of designs <li/>
+
−
<li>Leverage expertise from established engineering disciplines <li/>
+
−
<li>Ultimately, forward engineer novel solutions to diverse problems <li/>
+
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-4fa8302c-hudlvhna"  class="master item-box  gallery-box style-4fa8302c-ykkbejos    button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-4fa8302c-ykkbejos" data-preview-styleid='style-4fa8302c-ykkbejos' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-4fa8302c-ykkbejos content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-4fa8302c-ykkbejos" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-4fa8302c-hudlvhna" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-4fa8302c-ykkbejos" data-style="style-4e411-j0xhjgzynh" data-absolute-path=""  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-4fa8302c-wtxhhyba"  class="sub item-box  page-box style-4fa8302c-ykkbejos          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-4fa8302c-ykkbejos" data-preview-styleid='style-4fa8302c-ykkbejos' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-4fa8302c-wtxhhyba" data-preview-style="style-4fa8302c-ykkbejos" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="3024" data-orig-thumb-width="4032" data-vbid="vbid-4fa8302c-wtxhhyba" data-bgimg="https://static.igem.org/mediawiki/2017/8/87/C2V_Description_ArsLevels.jpg">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-left" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-4fa8302c-3bnrnrzv-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
 +
<div  id="vbid-4fa8302c-3bnrnrzv" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://static.igem.org/mediawiki/2017/8/87/C2V_Description_ArsLevels.jpg);"  data-orig-width="4032" data-orig-height="3024" >
 +
<!-- VIDEO TEMPLATE -->
 +
  
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
  
−
<div class="column full_size">
+
 
−
<h3>What was our motivation?</h3>
+
<!-- MAP TEMPLATE -->
−
<p>Because of the manufacturing and power plants in addition to other factors, Tacoma Washington has a lot of pollution specifically traces of arsenic.
+
−
Because of this, we developed a way to detect arsenic using our designed circuit to test soil and water samples <p/>
+
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 
 +
</div>
 +
 
</div>
 
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-4fa8302c-a7hrslal" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >arsenic levels of interest<br></h2>
 +
 
</div>
 
</div>
 +
<br class="lower-line-break" />
  
−
<ul>
+
−
<li> A clear and concise description of your project.</li>
+
−
<li>A detailed explanation of why your team chose to work on this particular project.</li>
+
−
<li>References and sources to document your research.</li>
+
−
<li>Use illustrations and other visual resources to explain your project.</li>
+
−
<ul/>
+
 +
<br class="upper-line-break" />
 +
<div class="preview-divider-holder removable-parent order-handle">
 +
<div id="vbid-4fa8302c-9he6h7nj" class="preview-element preview-divider magic-circle-holder quick-text-style-menu" data-menu-name="PREVIEW_DIVIDER">
 +
</div>
 +
</div>
 +
<br class="lower-line-break" />
  
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-4fa8302c-gttc6art" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">The maximum contaminant level for arsenic that federal and state agencies use as guideline to test the arsenic levels in drinking water was established by the EPA. Initially it was set to 50 micrograms/liter or 50 ppb. However, drinking water that contains 50 ppb arsenic for prolonged periods of time may cause bladder or lung cancer and other chronic conditions.</span></span></span></span><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;"></span></span></span></span><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;"> </span></span></span></span><br></p><p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;"></span></span></span></span><br></p><p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">In 2001, the EPA reduced the maximum contaminant level of arsenic to 10 micrograms/ liter or 10 ppb</span><span style="vertical-align: super; white-space: pre-wrap;">1</span><span style="vertical-align: baseline; white-space: pre-wrap;">. Nevertheless, according to data gathered between the years of 1993 and 2007, arsenic contamination of the waters in Washington state persisted</span><span style="vertical-align: super; white-space: pre-wrap;">2</span><span style="vertical-align: baseline; white-space: pre-wrap;">.</span></span></span></span><br></p><p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;"></span></span></span></span><br></p><p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">Probes in the waters of western part of Washington state, </span></span></span></span><span style="font-size: 16px; letter-spacing: 1.1px; white-space: pre-wrap; background-color: rgb(255, 255, 255);">near the southern part of the state by the Columbia River, and also north near Everett</span><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;"> have detected higher concentrations of arsenic, close to 50 ppb. </span></span></span></span><br></p><p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;"></span></span></span></span><br></p><p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">Developing an inexpensive paper-based circuit to detect the concentration of arsenic in the water in the region will greatly influence our knowledge and awareness of the quality of the water are consuming.</span></span><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">We hope to develop our assay to detect arsenic within the EPA limit.</span></span></span></span></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
  
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 
</div>
 
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-8f863eb8-qqcb9mt2"  class="master item-box  gallery-box style-8f863eb8-6rkb49z5    button-effects btn_hover3      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-8f863eb8-6rkb49z5" data-preview-styleid='style-8f863eb8-6rkb49z5' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-8f863eb8-6rkb49z5 content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-8f863eb8-6rkb49z5" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-8f863eb8-qqcb9mt2" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-8f863eb8-6rkb49z5" data-style="style-4e411-j0xhjgzynh" data-absolute-path=""  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-8f863eb8-3icuax6a"  class="sub item-box  page-box style-8f863eb8-6rkb49z5          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-8f863eb8-6rkb49z5" data-preview-styleid='style-8f863eb8-6rkb49z5' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-8f863eb8-3icuax6a" data-preview-style="style-8f863eb8-6rkb49z5" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-8f863eb8-3icuax6a" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
<div class="column full_size" >
+
<div id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
 +
<!-- VIDEO TEMPLATE -->
 +
  
−
<h5>Advice on writing your Project Description</h5>
+
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
  
−
<p>
 
−
We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
 
−
</p>
 
  
−
<p>
+
<!-- MAP TEMPLATE -->
−
Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
+
−
</p>
+
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
  
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 
</div>
 
</div>
 +
  
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-8f863eb8-7tigyyyk" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Sources</h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
  
−
<div class="column half_size" >
+
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-8f863eb8-rouzmtxs" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p></p><ol><li><span><span style="font-size: 12pt; font-family: Arial; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">“Drinking Water Arsenic Rule History”, EPA.gov. https://www.epa.gov/dwreginfo/drinking-water-arsenic-rule-history. Web. Sept 06, 2017.</span></span><br></li><li><span><span id="docs-internal-guid-1b7da29c-fdbd-fb12-e772-bbf347097214"><span style="font-size: 12pt; font-family: Arial; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">“Arsenic Detection in Washington State Public Water Supplies”, doh.wa.gov. http://www.doh.wa.gov/CommunityandEnvironment/DrinkingWater/Contaminants/ArsenicinDrinkingWater/MapofArsenicDetections. Web. Sept 06, 2017.</span></span></span></li></ol>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
  
−
<h5>References</h5>
+
−
<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
+
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- FOOTERS START -->
 +
<div  id="vbid-59a5b-mskg6fxi"  class="master item-box  footer-box style-59a5b-lbaqmrpi injected        " data-holder-type="footer"" data-holder-type="footer"  data-child-type="ORIGIN"  data-styleid="style-59a5b-lbaqmrpi" data-preview-styleid='style-59a5b-lbaqmrpi' data-preset-type-id="FOOTERS">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="footer-wrapper item-wrapper footers-wrapper ">
 +
 +
<div class="item-content leaf middle_layout footer content shrinker-parent" data-self="vbid-59a5b-mskg6fxi" data-preview-style="style-59a5b-lbaqmrpi" data-style="style-f785e-jp5ywgaa0t" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-59a5b-mskg6fxi" data-bgimg="">
 +
<div  class="preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
 +
<div  id="no-image" data-title='click to Add'  class="background-div  normal preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="PREVIEW_IMAGE" style="" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 
 +
 
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 
 +
</div>
 +
<div class="helper-div">
 +
<div class="benet" style="min-height:inherit;"></div>
 +
<div class="item-details preview-content-wrapper  middle">
 +
<div class="preview-content-holder shrinker-content">
 +
<!-- ICON TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 
 +
<!-- TITLE TEMPLATE -->
 +
 +
<div class="preview-title-holder removable-parent">
 +
 +
<h2 id="vbid-59a5b-xinhfnbg" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Follow us on Social Media!</h2>
 +
 +
</div>
 +
<br />
 +
 
 +
<!-- SUBTITLE TEMPLATE -->
 +
 +
<div class="preview-subtitle-holder removable-parent">
 +
 +
<h3 id="vbid-59a5b-novarlkx" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">2304 Jefferson Ave, Tacoma, WA 98402</h3>
  
 
</div>
 
</div>
 +
<br />
  
 +
<!-- BODY TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='BODY' style="display:none;"></div>
  
−
<div class="column half_size" >
+
<!-- PRICE TEMPLATE -->
−
<h5>Inspiration</h5>
+
−
<p>See how other teams have described and presented their projects: </p>
+
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
  
−
<ul>
+
<!-- FORM TEMPLATE -->
−
<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
+
<div class="preview-form">
−
<li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
+
 
−
<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
+
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
−
<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
+
−
</ul>
+
 
</div>
 
</div>
 +
<!-- LINKS TEMPLATE -->
 +
<div class="preview-item-links ">
 +
<div class="preview-links-wrapper">
  
  
  
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
<!-- SOCIAL TEMPLATE -->
 +
 +
<div class="preview-social-wrapper removable-parent"  >
 +
<div id="vbid-59a5b-xmkwjkjw" class="preview-element preview-social-holder magic-circle-holder" data-menu-name="PREVIEW_SOCIAL" data-theme="7" >
 +
 +
<div id="FACEBOOK" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="FACEBOOK" data-img-url="https://static.igem.org/mediawiki/2017/a/a4/FacebookImage.png">
 +
<a class='social-link-url' href="https://www.facebook.com/RAINIncubator" target='_blank'><img class='preview-link-img ' src="https://static.igem.org/mediawiki/2017/a/a4/FacebookImage.png" ></a>
 +
</div>
 +
 +
<div id="TWITTER" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="TWITTER" data-img-url="https://static.igem.org/mediawiki/2017/5/59/TwitterImage.png">
 +
<a class='social-link-url' href="https://twitter.com/RAINincubator" target='_blank'><img class='preview-link-img ' src="https://static.igem.org/mediawiki/2017/5/59/TwitterImage.png" ></a>
 +
</div>
 +
 +
<div id="INSTAGRAM" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="INSTAGRAM" data-img-url="https://static.igem.org/mediawiki/2017/8/86/InstagramImage.png">
 +
<a class='social-link-url' href="https://www.linkedin.com/in/rain-network" target='_blank'><img class='preview-link-img ' src="https://static.igem.org/mediawiki/2017/8/86/InstagramImage.png" ></a>
 +
</div>
 +
 +
<div id="YOUTUBE" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="YOUTUBE" data-img-url="https://static.igem.org/mediawiki/2017/3/34/YoutubeImage.png">
 +
<a class='social-link-url' href="https://www.youtube.com/channel/UCuVc4cKxuUzpjZfywBgoT9w" target='_blank'><img class='preview-link-img ' src="https://static.igem.org/mediawiki/2017/3/34/YoutubeImage.png" ></a>
 +
</div>
 +
 +
<div id="GOOGLE_PLUS" class='link-entry' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="GOOGLE_PLUS" data-img-url="https://static.igem.org/mediawiki/2017/f/fa/GooglePlusImage.png">
 +
<a class='social-link-url' href="https://plus.google.com/u/0/+RainincubatorOrg" target='_blank'><img class='preview-link-img ' src="https://static.igem.org/mediawiki/2017/f/fa/GooglePlusImage.png" ></a>
 +
</div>
 +
 +
</div>
 +
</div>
 +
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="middle"></div>
 +
 +
</div>
 +
</div>
 +
<!-- FOOTERS END -->
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="light-box-wrapper  space-layer" style="display:none;">
 +
<div class="light-box-image-holder">
 +
<div class="light-box-image animated">
 +
 +
</div>
 +
<div class="lightbox-text-wrapper ">
 +
<div class="lightbox-text-holder animated">
 +
<div class="lightbox-title"></div>
 +
<div class="lightbox-subtitle"></div>
 +
<div id="paginator" style="display:none;">
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
      <img src="https://static.igem.org/mediawiki/2017/d/db/Arrow1.png" class="download-gallery-btn clickable" style="display: none;">
 +
<img src="https://static.igem.org/mediawiki/2017/9/9e/XImage1.png" class="close-lightbox-btn clickable" style="opacity: 1;">
 +
<img src="https://2017.igem.org/File:Thing5.png" class="lightbox-arrow lightbox-left clickable top-layer" >
 +
<img src="https://2017.igem.org/File:Thing4.png" class="lightbox-arrow lightbox-right clickable top-layer" >
 +
</div>
 +
 +
</div>
 +
 +
<script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/lightbox?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/spimeengine?action=raw&ctype=text/javascript"></script>
 +
 +
 +
<!--  SHOPROCKET  ECOMMERCE  -->
 +
<script>
 +
var JSR = jQuery.noConflict();
 +
var $ = jQuery.noConflict();
 +
</script>
 +
 +
<input type="hidden" name="sr-companyid" id="sr-companyid" value="">
 +
</body>
 +
 +
 
</html>
 
</html>

Latest revision as of 16:05, 1 November 2017

HOME
TEAM
PROJECT
Description
Design
Experiments
Notebook
Notebook Overview
June Notebook
July Notebook
August Notebook
September Notebook
October Notebook
InterLab
Results
Demonstrate
Improve
Attributions
PARTS
Parts
Basic Parts
Composite Parts
SAFETY
HUMAN PRACTICES
Silver HP
Integrated and Gold
Public Engagement
AWARDS
Hardware
Environmental Project
JUDGING FORM


Description - Home


DESCRIPTION OF OUR PROJECT



The goal of this project is to engineer a biological circuit that utilizes E. coli plasmid DNA sequences and a green fluorescent protein (GFP) reporter to detect arsenic. The constitutive promoter in our construct produces a regulatory protein ArsR that binds with arsenic ions and triggers visible fluorescence. Our team will demonstrate this circuit using a cell-free lateral flow paper-based system. Ultimately, the purpose of this circuit will be to provide an inexpensive and ubiquitous arsenic sensor for testing environmental samples. ​



Goal #1



Design and create a synthetic biological circuit containing a reporter protein regulated by arsenic ions.



Goal #2



Demonstrate that the circuit can detect arsenic in solution in cells or using cell-free lysates.



Goal #3



Determine the conditions needed for cell-free lysates to detect EPA limits for harmful concentrations of arsenic.



why we chose arsenic





Arsenic was selected by Cadets2Vets as the first chemical we would attempt to detect using a biological circuit. It is naturally found in the environment, especially in groundwater, and around industrial plants as a byproduct of manufacturing practices.​


The detection of arsenic is especially important to team members in the Puget Sound area, because North Tacoma is a Superfund Site. The ASARCO smelting company smokestack, erected in 1917, was a symbol of economic prosperity and booming business in the developing city of Tacoma. The copper smelting site on Ruston’s shore in Puget Sound brought jobs and wealth to the area. Unfortunately, over the course of its 100 years of operation, the plant had been pumping toxic materials like arsenic and lead directly into the air, where it then settled into the surrounding soil and water of the greater Tacoma area.


The smokestack was demolished in 1985, but there are still toxic levels of heavy metals in the soil over 30 years later. The city of Tacoma takes measures to warn new Tacoma homeowners about how to mitigate any increased levels of exposure to toxic materials in their soil, but must also take more drastic steps to protect users of publicly-owned land.

The Department of Defense has a strong interest in developing rapid assays to test for environmental risks to protect the health of service members. Troops often deploy to hostile environments and assessing the risks of the environment itself can be challenging. The development of the arsenic circuit represents a new way to use synthetic biology in the military setting. Demonstrating that a biological circuit can be used in this application will lead to an expansion of the technology to test for more substances in the future.



arsenic levels of interest





The maximum contaminant level for arsenic that federal and state agencies use as guideline to test the arsenic levels in drinking water was established by the EPA. Initially it was set to 50 micrograms/liter or 50 ppb. However, drinking water that contains 50 ppb arsenic for prolonged periods of time may cause bladder or lung cancer and other chronic conditions.


In 2001, the EPA reduced the maximum contaminant level of arsenic to 10 micrograms/ liter or 10 ppb1. Nevertheless, according to data gathered between the years of 1993 and 2007, arsenic contamination of the waters in Washington state persisted2.


Probes in the waters of western part of Washington state, near the southern part of the state by the Columbia River, and also north near Everett have detected higher concentrations of arsenic, close to 50 ppb.


Developing an inexpensive paper-based circuit to detect the concentration of arsenic in the water in the region will greatly influence our knowledge and awareness of the quality of the water are consuming.We hope to develop our assay to detect arsenic within the EPA limit.



Sources



  1. “Drinking Water Arsenic Rule History”, EPA.gov. https://www.epa.gov/dwreginfo/drinking-water-arsenic-rule-history. Web. Sept 06, 2017.
  2. “Arsenic Detection in Washington State Public Water Supplies”, doh.wa.gov. http://www.doh.wa.gov/CommunityandEnvironment/DrinkingWater/Contaminants/ArsenicinDrinkingWater/MapofArsenicDetections. Web. Sept 06, 2017.

Follow us on Social Media!


2304 Jefferson Ave, Tacoma, WA 98402