Difference between revisions of "Team:Cadets2Vets/Description"

 
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{{Cadets2Vets}}
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{{Team:Cadets2Vets/SideMenu}}
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<html>
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<head>
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<html>
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<head>
 
<title>
 
<title>
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Experiments - USE THIS ONE!!!</title>
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Description - Home</title>
 
<!-- META -->
 
<!-- META -->
 
<meta charset="UTF-8">
 
<meta charset="UTF-8">
 
<meta name="viewport" content="width=device-width, maximum-scale=1"/>  
 
<meta name="viewport" content="width=device-width, maximum-scale=1"/>  
 
 
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<!-- CSS -->
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<link href="https://2017.igem.org/Team:Cadets2Vets/css/common?action=raw&ctype=text/css" rel="stylesheet">
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                <link href="https://2017.igem.org/Team:Cadets2Vets/css/all_css?action=raw&ctype=text/css" rel="stylesheet">
 
 
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<meta name="robots" content="noindex, nofollow">
 
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<script src="https://www.youtube.com/iframe_api"></script>
 
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<!-- CSS -->
 
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<link rel="stylesheet" href="//edit.www.imcreator.com/css/common.css?v=1.4.0p1">
 
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<link rel="stylesheet" href="//edit.www.imcreator.com/js/lib/sweetalert/sweetalert.css" />
 
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<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/all_css.css?v=1.4.0p1" />
 
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<link rel="icon" type="image/png" href="https://lh3.googleusercontent.com/dd_e0xA19up9208Tv6odcjHEw6z4cKAA6fTgjZ9ynkKoSnr5R4vFxI7gZp6pnZH1Vi5T5f-fphjFsrLG=s30" />
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<link rel="icon" type="image/png" href="https://static.igem.org/mediawiki/2017/7/71/Circleimage1.png" />
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<link rel="apple-touch-icon" href="https://lh3.googleusercontent.com/dd_e0xA19up9208Tv6odcjHEw6z4cKAA6fTgjZ9ynkKoSnr5R4vFxI7gZp6pnZH1Vi5T5f-fphjFsrLG=s30" />
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<link rel="apple-touch-icon" href="https://static.igem.org/mediawiki/2017/7/71/Circleimage1.png" />
 
 
 
 
 
 
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<link rel="stylesheet" type="text/css" href="https://maxcdn.bootstrapcdn.com/font-awesome/4.7.0/css/font-awesome.min.css" />
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<link id="vbid-59a5b-fnxcttvm-STRIPE_DATA" link href="https://2017.igem.org/Team:Cadets2Vets/css/stripe_data_css?action=raw&ctype=text/css" rel="stylesheet">
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<link id="style-59a5b-mfwumtzk" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-59a5b-mfwumtzk&viewer=xprs&parent_style=style-59a5b-mfwumtzk&v=1.4.0p1">
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<link id="style-59a5b-ecga9z2y" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-59a5b-ecga9z2y&viewer=xprs&parent_style=style-59a5b-mfwumtzk&v=1.4.0p1">
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<link id="style-59a5b-up4vqblq" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-59a5b-up4vqblq&viewer=xprs&parent_style=style-59a5b-mfwumtzk&v=1.4.0p1">
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<link id="style-59a5b-mbbodavg" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-59a5b-mbbodavg&viewer=xprs&parent_style=style-59a5b-mfwumtzk&v=1.4.0p1">
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<link id="style-59a5b-2upaool4" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-59a5b-2upaool4&viewer=xprs&parent_style=style-59a5b-mfwumtzk&v=1.4.0p1">
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<link id="style-59a5b-lbaqmrpi" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-59a5b-lbaqmrpi&viewer=xprs&parent_style=style-59a5b-mfwumtzk&v=1.4.0p1">
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<link id="style-59a5b-rzwawvht" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-59a5b-rzwawvht&viewer=xprs&parent_style=style-59a5b-mfwumtzk&v=1.4.0p1">
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<link id="style-59a5b-jbqq3wgo" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-59a5b-jbqq3wgo&viewer=xprs&parent_style=style-59a5b-mfwumtzk&v=1.4.0p1">
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<link id="style-59a5b-yamofliu" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_dynamic_css?style_id=style-59a5b-yamofliu&viewer=xprs&parent_style=style-59a5b-mfwumtzk&v=1.4.0p1">
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<link id="vbid-59a5b-l5xeq0z6-STRIPE_DATA" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_data_css?page_id=vbid-59a5b-l5xeq0z6-STRIPE_DATA&v=1.4.0p1">  
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<link id="vbid-59a5b-fnxcttvm-STRIPE_DATA" rel="stylesheet" type="text/css"  href="//edit.www.imcreator.com/stripe_data_css?page_id=vbid-59a5b-fnxcttvm-STRIPE_DATA&v=1.4.0p1">  
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<link id="vbid-04d15a56-3o81aqko-STRIPE_DATA" link href="https://2017.igem.org/Team:Cadets2Vets/css/description/stripe_data_css?action=raw&ctype=text/css" rel="stylesheet">
 
 
−
<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/css/fonts.css?v=1.4.0p1" />
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<link href="https://2017.igem.org/Team:Cadets2Vets/css/fonts?action=raw&ctype=text/css" rel="stylesheet">
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<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/css/effects.css?v=1.4.0p1" />
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                <link href="https://2017.igem.org/Team:Cadets2Vets/css/effects?action=raw&ctype=text/css" rel="stylesheet">
−
<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/css/lightbox.css?v=1.4.0p1">
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                <link href="https://2017.igem.org/Team:Cadets2Vets/css/lightbox?action=raw&ctype=text/css" rel="stylesheet">
 +
                <link href="https://2017.igem.org/Team:Cadets2Vets/css/spimeview?action=raw&ctype=text/css" rel="stylesheet">
 
 
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<link rel="stylesheet" type="text/css" href="//edit.www.imcreator.com/css/spimeview.css?v=1.4.0p1">
 
 
 
 +
<link id="vbid-04d15a56-3o81aqko-STATIC_STYLE" link href="https://2017.igem.org/Team:Cadets2Vets/css/description/static_style?action=raw&ctype=text/css" rel="stylesheet">
 
 
 
<!-- SCRIPT -->
 
<!-- SCRIPT -->
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<script src="https://code.jquery.com/jquery-2.x-git.min.js" type="text/javascript"></script>
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<script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/xprs_helper?action=raw&ctype=text/javascript"></script>
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<script src="https://maps.googleapis.com/maps/api/js?key=AIzaSyByWRZWFbQRxJM7SiNz2NURPDPJPqTtBQQ" type="text/javascript"></script>
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<script src="//edit.www.imcreator.com/js/xprs_helper.js?v=1.4.0p1" type="text/javascript"></script>
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<script src="//edit.www.imcreator.com/js/lib/jquery.cookie.min.js" type="text/javascript"></script>
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<script src="//edit.www.imcreator.com/js/lib/sweetalert/sweetalert.min.js" type="text/javascript"></script>
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<script src="//edit.www.imcreator.com/translation_js?lang=en" type="text/javascript"></script>
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<!-- User Head Code -->
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<script src="https://imos004-dot-im--os.appspot.com/js/imos.js?v=1.4.0p1" type="text/javascript"></script>
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<script type="text/javascript" src="//edit.www.imcreator.com/all_js.js?v=1.4.0p1"></script>
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<script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/all_js?action=raw&ctype=text/javascript"></script>
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<script src="//edit.www.imcreator.com/js/lib/touchswipe/jquery.mobile.custom.min.js"></script>
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<script type="text/javascript" src="https://2017.igem.org/Team:Cadets2Vets/js/lib/touchswipe/jquery_mobile_custom_min?action=raw&ctype=text/javascript"></script>
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<script type="text/javascript">IMOS.pageView();</script>
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<!-- User analytics -->
 
<!-- User analytics -->
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<script type="text/javascript">
 
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  var _gaq = _gaq || [];
 
−
  _gaq.push(['_setAccount', '']);
 
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  _gaq.push(['_trackPageview']);
 
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  (function() {
 
−
    var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true;
 
−
    ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js';
 
−
    var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s);
 
−
  })();
 
−
</script>
 
  
  
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<!-- Serving analytics -->
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<script>
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var currentDomain =  window.location.host;
 +
if (currentDomain != "localhost:7000"){
 +
var _gaq = _gaq || [];
 +
_gaq.push(['_setAccount', 'UA-58695071-1']);
 +
_gaq.push(['_setDomainName', currentDomain]);
 +
_gaq.push(['_setAllowLinker', true]);
 +
_gaq.push(['_trackPageview']);
 +
}
 +
</script>
 +
 
<!-- Label config-->
 
<!-- Label config-->
 
<script>
 
<script>
 
 
−
var LABEL_CONFIG = JSON.parse('{"LANDING_PAGE": "fast_homepage", "BILLING": {"BUY_NOW_URL": "none", "BUY_NOW_IMAGE": "none", "PAYPAL_RECURRENCES": "1", "CUSTOM_CURRENCY": "USD", "HIDE_FREE_URL": "off", "PAYPAL_ID": "", "PAYPAL_AMOUNT": "100", "CUSTOM_TITLE": "Single Commercial License", "PAYPAL_PERIOD": "Y", "CUSTOM_EXPLAINED": "single domain connection for a single site. unlimited bandwidth, unlimited storage, premium support, e-commerce", "BILLING_TYPE": "abtest", "PAYPAL_OFFER_NAME": "Premium Subscription", "PAYPAL_EXPLAINED": "single domain connection for a single site. unlimited bandwidth, unlimited storage, premium support, e-commerce", "CUSTOM_CYCLE": "/month paid annualy", "PAYPAL_CURRENCY": "USD", "CUSTOM_PRICE": "100"}, "BRANDING": {"BRAND_THEMES_LOGO": "none", "BRAND_SECONDARY_COLOR": "rgb(255,255,255)", "BRAND_MAIN_COLOR": "#0099CC", "BRAND_BG": "https://lh3.googleusercontent.com/ZgLp2jeL7ynJJZ-aMv-LgcFLv1-wuV0E4mMxsQXqyPe9CDHiClIlQODrGLalwzQp4XNWjMBy8Z68369P4Q", "BUTTON_HOVER_COLOR": "#0099cc", "BRAND_SMALL_LOGO": "https://lh3.googleusercontent.com/PVw5qfegr3TcOhyx6UH2QShnmtTexxEIuzECuxiKnbfkzYPv28lrzSMSnelUe7nkoYjs-KLxOYzo1wgOSZk", "BRAND_LARGE_LOGO": "https://lh3.googleusercontent.com/BVPCuhW88_eSWMXpfFE8ZZh9IxRktAzfOp_rZ9vvhH2zLhLNoCJ6TjOleEnqppMxvYHdvsxDX-uXWfRrgQ"}, "SUPPORT": {"SUPPORT_URL": "http://support.imxprs.com/hc/en-us/requests/new", "VIDEO_ID": "K1ZnSLe-Rdk", "SUPPORT_ID": "", "KNOWLEDGE_CENTER_URL": "http://support.imxprs.com/hc/en-us"}, "ANALYTICS_ID": "UA-64970204-1", "META": {"OWNER": "xprsxprs", "DOMAIN": "imcreator.com", "CREATION_DATE": "28-07-2015 13:34:42", "PRODUCT_NAME": "XPRS", "NAME": "xprs"}, "DOMAIN_BILLING": {"BUY_NOW_URL": "none", "PAYPAL_RECURRENCES": "1", "CUSTOM_CURRENCY": "USD", "PAYPAL_PERIOD": "Y", "PAYPAL_AMOUNT": "100", "PAYPAL_ID": "", "CUSTOM_EXPLAINED": "1-Year domain subscription", "BILLING_TYPE": "standard", "PAYPAL_OFFER_NAME": "Premium Subscription", "PAYPAL_CURRENCY": "USD", "CUSTOM_PRICE": "100"}, "ID": "label-f6d7c", "SETTINGS": {"ALLOW_PERIPHERAL_PAGES": "allow", "LOGIN_URL": "", "ALLOW_ECOMMERCE": "allow", "ALLOW_NEW_DOMAIN": "allow", "ENABLE_IMXPRT": "true", "ECOMMERCE_DASHBOARD": "http://dashboard.shoprocket.co", "INCLUDE_REGISTER_PHONE": "disallow", "CUSTOM_TEMPLATES": "default", "API_TOKEN": "NOT GENERATED", "ALLOW_CALL_ME_CHECKBOX": "disallow", "SR_PARTNER_KEY": "N/A", "PHONE_EDIT_APP_LINK": "https://itunes.apple.com/app/apple-store/id953999749?pt=1847141&mt=8", "DEFAULT_EDITOR_LANG": "en", "CUSTOM_SECTIONS": "default", "ALLOW_PHONE_EDIT": "allow", "ALLOW_NEW_SITES": "allow", "REGISTER_URL": "", "BILLING_SERVER": "", "USER_PROFILE": "show", "REGISTER_STEP": "themes", "ENABLE_IMOS": "false", "ECOMMERCE_SOLUTION": "IMOS", "WEBHOOKS_SERVER": "", "ALLOW_RESELLER": "allow"}, "HIY": "false", "EMAILS": {"FORWARD_ALL_EMAILS": "off", "FORWARD_EMAIL": ""}, "SERVERS": {"HEROKU_APP": "", "S3_ACCESS": "", "S3_REGION": "", "UPLOAD_TO_S3": "off", "S3_SECRET": "", "S3_BUCKET": ""}, "TOS_URL": "http://www.imcreator.com/terms-of-service"}');
+
var LABEL_CONFIG = {};
 
 
 
 
 
</script></head>
 
</script></head>
  
−
<body class="fast-animated-bg fixed-bg " data-ecommerce-solution="NONE" data-root-id="vbid-59a5b-fnxcttvm" data-root-style-id="style-59a5b-yamofliu" data-default-currency="USD" data-osid="osid--6af0e67f-af8681d1"  data-app-version="1.4.0p1" data-caller="preview"  data-ecommerce-dashboard="http://dashboard.shoprocket.co" data-static-server="//edit.www.imcreator.com" data-imos-server="https://imos004-dot-im--os.appspot.com">
+
<body class="fast-animated-bg fixed-bg " data-ecommerce-solution="NONE" data-root-id="vbid-59a5b-fnxcttvm" data-root-style-id="style-59a5b-yamofliu" data-default-currency="USD" data-osid="osid--6af0e67f-af8681d1"  data-app-version="1.4.1f" data-caller="live"  data-ecommerce-dashboard="https://dashboard.shoprocket.co" data-static-server="//www.imcreator.com" data-imos-server="https://imos004-dot-im--os.appspot.com">
 
 
−
<div id="xprs" data-website-name="USE THIS ONE!!!" class="xprs-holder" >
+
<div id="xprs" data-website-name="Home" class="xprs-holder" >
 
<div class="main-page "  >
 
<div class="main-page "  >
 
<div id="content">
 
<div id="content">
−
<div  id="vbid-59a5b-l5xeq0z6"  class="master container style-59a5b-mfwumtzk content stripes  website-style " data-itemtype="folder" data-creator="" data-itemname="Experiments"  data-itemslug="experiments" data-itemstyleid="style-59a5b-mfwumtzk" data-margintop="" data-arranger="stripes" data-layout="middle" data-vbid="vbid-59a5b-l5xeq0z6" data-preset-type-id="" data-preview-style="style-59a5b-mfwumtzk" data-style="style-7f715-gkcpu3e69m" data-absolute-path="http://edit.www.imcreator.com/viewer" >
+
<div  id="vbid-04d15a56-3o81aqko"  class="master container style-04d15a56-okeof5tv content stripes  website-style " data-itemtype="folder" data-creator="" data-itemname="Description"  data-itemslug="description" data-itemstyleid="style-04d15a56-okeof5tv" data-margintop="" data-arranger="stripes" data-layout="middle" data-vbid="vbid-04d15a56-3o81aqko" data-preset-type-id="" data-preview-style="style-04d15a56-okeof5tv" data-style="style-7f715-gkcpu3e69m" data-absolute-path="" >
 
<!-- ARRANGER SETTINGS -->
 
<!-- ARRANGER SETTINGS -->
 
<div class="arranger-settings" style="display:none;"
 
<div class="arranger-settings" style="display:none;"
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<!-- TITLE TEMPLATE -->
 
<!-- TITLE TEMPLATE -->
 
 
−
<div class="preview-title-holder removable-parent">
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<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
−
+
−
<h2 id="vbid-59a5b-0dca3gi2" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Menu</h2>
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−
+
−
</div>
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−
<br />
+
  
 
<!-- SUBTITLE TEMPLATE -->
 
<!-- SUBTITLE TEMPLATE -->
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<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-qpimjeag" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="index.html" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="vbid-59a5b-w5gehayb"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Team Members</span>
+
<span id="element-a1a56421418f7ce"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Home</span>
 
</a>
 
</a>
 
 
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<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-l5xeq0z6" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="collaborations.html" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="vbid-59a5b-9i6v7wly"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Projects</span>
+
<span id="element-5efad87d43e4352"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Collaborations</span>
 
</a>
 
</a>
 
 
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<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-xtfijafb" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="description.html" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="vbid-59a5b-b9vovddz"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Parts</span>
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<span id="element-e6e2afb357139e4"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Description</span>
 
</a>
 
</a>
 
 
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<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-bb9vg46d" data-link-type="EXISTING"  target="_self" >
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<a class="removable-parent" href="design.html" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="vbid-59a5b-hlhlmo7j"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Safety</span>
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<span id="element-c6287513fca8901"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Design</span>
 
</a>
 
</a>
 
 
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<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-hjemejig" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="experiments.html" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="vbid-59a5b-k2taajbu"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Human Practices</span>
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<span id="element-f4b485a3e8b3825"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Experiments</span>
 
</a>
 
</a>
 
 
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<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-0rawt2n3" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="notebook.html" data-link-type="EXISTING"  target="_self" >
 
 
−
<span id="vbid-59a5b-dv55z3u4"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Awards</span>
+
<span id="element-603d2303312c4bd"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook</span>
 
</a>
 
</a>
 
 
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<li class="removable-parent">
 
<li class="removable-parent">
 
 
−
<a class="removable-parent" href="http://edit.www.imcreator.com/viewer/vbid-59a5b-fnxcttvm/vbid-59a5b-szkh4ovx" data-link-type="EXISTING"  target="_self" >
+
<a class="removable-parent" href="notebook-august.html" data-link-type="EXISTING"  target="_self" >
 
 
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−
<p>The goal of this project is to engineer a biological circuit utilizing multiple <i>E. coli</i> DNA regulatory sequences and a green fluorescent protein (GFP) reporter as a means to detect arsenic. Once this construct is designed and constructed the circuit will be tested to demonstrate its viability using a cell free lateral flow paper based system designed to detect arsenic.</p>
 
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+
−
<li class="ui-widget-content item" value="vbid-a86ce9b6-2qvkpfd1" style="background-position: center;background-image:url('https://lh3.googleusercontent.com/DOnLWB4PeczAQPeWWpq9voKMb1mVkgRpWpxm7uX_yjoLagAxzIJFauWVHQqqzW4bFaD0mKhWowYPDDHldA=s250')"></li>
+
−
<li class="ui-widget-content item" value="vbid-b7756e4f-gq2iu6ee" style="background-position: center;background-image:url('https://lh3.googleusercontent.com/WCR2bG62NvuH6t9gN280oemGW8JYIZnVt_UFC3wM3ZRCdkl5Po619_Tfgb9pglMGPiFwQhwgIxdoqDlS=s250')"></li>
+
−
<li class="ui-widget-content item" value="vbid-57286964-gq2iu6ee" style="background-position: center;background-image:url('https://lh3.googleusercontent.com/P1eBKYoYrGaj3ZV7swtXte706n4jfuIqTRvuNDTljpOZUBhgArr3uD0yZSoJWe7cqM19fEfELNC0DVFJkQ=s250')"></li>
+
−
<li class="ui-widget-content item" value="vbid-627dc7e6-s5pdf8f2" style="background-position: center;background-image:url('https://lh3.googleusercontent.com/rrrM4d3RIw5wYJ1TAo0gt0G09mwJ5sXLvAQt8zTBl8aCs_BJXP4zH_lvfVE3y-J-zek5-i9NmR-mv8zW=s250')"></li>
+
−
<li class="ui-widget-content item" value="vbid-8ff732dd-abrgqdbx" style="background-position: center;background-image:url('https://lh3.googleusercontent.com/tBT3-1uAyuGx4N7g9L9izG6TZoVf96Ly-9OgvTBJaTRqLWGOiIizej9ekbjKnyD7BmaAcJcw1WYHap_M=s250')"></li>
+
−
<li class="ui-widget-content item" value="vbid-466845d0-iidd4ogs" style="background-position: center;background-image:url('https://lh3.googleusercontent.com/ApteRE0sFclf1R2YJCTxGfXtgTdkKaTqj0KpFD0vwfmOxQyhMKGlUqFKm5pzcor1yd99aM72ygnUxVAVVg=s250')"></li>
+
−
<li class="ui-widget-content item" value="vbid-dd9c4df1-u8eh7xv3" style="background-position: center;background-image:url('https://lh3.googleusercontent.com/9xxMvka-ASkV2HFYfqn09hrBvTnhpdSt8tw53ULB19ZXhhSR0DNGCc4ON4zrvXGiin2U309HL-Psqa_m=s250')"></li>
+
−
<li class="ui-widget-content item" value="vbid-e59d5107-kqgmxt5n" style="background-position: center;background-image:url('https://lh3.googleusercontent.com/7s2eqiLGtVW2cKq9PmypBj7DPgK1QTI1M0TqKCyIjpk1Wm8uTjGgUs2vV6glSoA90MBYOCGWxW0vQ9Ri-S4=s250')"></li>
+
−
<li class="ui-widget-content item" value="vbid-05fbf368-36hyxloh" style="background-position: center;background-image:url('https://lh3.googleusercontent.com/j2UVF0-_NqZitrzY7Jw_dB8NwbKR_Oe6jt8nV8w2PG3v4N-MSxqrJiAnmBBrxSl88vXEuuOr44pZFjT_=s250')"></li>
+
−
<li class="ui-widget-content item" value="vbid-71c36603-wgdrzlzs" style="background-position: center;background-image:url('https://lh3.googleusercontent.com/MHyRWCeEK8VShirpKgHhNHaEE5xWhpEYJwUOU493peeHFU_140TE0QYHOc3SYxFXmTXbslSda5Kor5cyMQ=s250')"></li>
+
−
<li class="ui-widget-content item" value="vbid-275d0285-tpvaafiz" style="background-position: center;background-image:url('https://lh3.googleusercontent.com/gf1RbaxV8A9eXPzZm1xdosCEBJpTtwRs09VeEezhMlz86t0t1iNpXyzOhld_2-btPD8pnTBjlkAfzaM5kQ=s250')"></li>
+
−
<li class="ui-widget-content item" value="vbid-ae924c98-yeqviu8q" style="background-position: center;background-image:url('https://lh3.googleusercontent.com/uD8xDAgVKM8wavlw9JwcE1UNMKU-9rSaZ8HlRayRNRw90JClCJRo3_3Cj-zez000qwXpNqA2DXm-KttA2KY=s250')"></li>
+
−
</ol>
+
−
</div>
+
−
<div id='template_params' style="padding: 15px;">
+
−
<!--<label class='app_text_label' style="width:103px;margin-left: -8px;">Email for replies: </label>
+
−
<input class='app_field_input ' type="text" name="data_href" style="width: 265px;" placeholder="your@email.com"><br>
+
−
<textarea class='app_field_input ' style="width: 265px; height:50px" type="text" rows="3" name="data_text"  placeholder="Text to be presented after form submission"></textarea>
+
−
-->
+
−
<label class='app_text_label' style="width:103px;margin-left: -8px;">Location: </label>
+
−
  <select class='app_field_input' style="width:150px" name="locationOption" onChange="locationSelected(this.value)">
+
−
  <option value="center">Centered</option>
+
−
  <option value="bottom_right">Bottom Right</option>
+
−
  <option value="fullscreen">Fullscreen</option>
+
−
</select>
+
−
<br>
+
−
 
+
−
<label class='app_text_label short_label' name="popupWidth">Width </label>
+
−
<input class='app_field_input short_field' type="number" name="popupWidth" style="width:70px">
+
−
+
−
<label class='app_text_label short_label' name="popupHeight" style="margin-left: 35px;">Height </label>
+
−
<input class='app_field_input short_field' type="number" name="popupHeight" style="width:70px"><br><br>
+
 
 
−
<label class='app_text_label' style="width:8em;margin-left: -8px;">Pop Options: </label><br>
+
<a class="removable-parent" href="demonstrate.html" data-link-type="EXISTING"   target="_self" >
−
<input class='app_field_input' type="checkbox" id="timer" name="popingOptions" checked><label class='app_text_label' style="margin-left: 6px;margin-right: 0px;margin-top: 5px;">Timer </label>
+
−
<input class='app_field_input' type="checkbox" id="on_scroll" name="popingOptions"><label class='app_text_label' style="margin-left: 6px">On Scroll </label>
+
−
<input class='app_field_input' type="checkbox" id="on_exit" name="popingOptions" style="margin-left: 6px;"><label class='app_text_label' style="margin-left: 6px;margin-right: 0px;">On Exit </label>
+
−
<br>
+
 
 
−
<div class="extra_options timer" style="display:none">
+
<span id="1ad7c153"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Demonstrate</span>
−
<label class='app_text_label long_label' style='margin-right: 5px;'>Pop after</label>
+
</a>
−
<input class='app_field_input short_field' type="number" name="timeToPopup">
+
−
<label class='app_text_label long_label' style='width:67px;margin-left: 5px;'> seconds</label><br>
+
</li>
−
</div>
+
−
+
−
<div class="extra_options on_scroll" style="display:none">
+
−
<label class='app_text_label long_label' style='margin-right: 5px;'>Pop after scrolling</label>
+
−
<input class='app_field_input short_field' type="number" name="endPrecent">
+
−
<label class='app_text_label long_label' style='width: 77px;margin-left: 5px;'> % of the page</label><br>
+
−
</div>
+
−
+
−
<!--  
+
−
<div class="extra_options on_exit" style="display:none">
+
−
<label class='app_text_label long_label' style="margin-top: 5px;">Every page Reload </label>
+
−
<input class='app_field_input' type="checkbox" name="aggressive"><br>
+
−
</div>
+
−
  -->
+
−
</div>
+
−
</div>
+
  
−
<script>
+
−
 
+
−
var/*<{*/popupWidth/*}*/ = /*{*/1200/*}>*/;
+
−
var/*<{*/popupHeight/*}*/ = /*{*/600/*}>*/;
+
−
var /*<{*/timeToPopup/*}*/ = /*{*/5/*}>*/; //in seconds
+
−
var /*<{*/endPrecent/*}*/ = /*{*/80/*}>*/;
+
−
var /*<{*/popingOptions/*}*/ = /*{*/["timer"]/*}>*/;
+
−
var /*<{*/locationOption/*}*/ = /*{*/"center"/*}>*/;
+
−
var /*<{*/template/*}*/ = /*{*/"vbid-3726659f-w2ypdu1q"/*}>*/;
+
−
var /*<{*/data_href/*}*/ = /*{*/""/*}>*/;
+
−
var /*<{*/data_text/*}*/ = /*{*/""/*}>*/;
+
−
 
+
−
var stripeObj;
+
−
var backgroundObj;
+
−
 
+
−
var gDidPop = false;
+
−
 
+
−
if (typeof EditorHelper == "undefined"){
+
−
if (popingOptions.indexOf("timer") != -1) {
+
−
document.addEventListener('DOMContentLoaded', setTimeout(popUpStripe, timeToPopup*1000));
+
−
}
+
−
} else {
+
−
document.addEventListener('xprs-app-loaded', popUpStripe);
+
−
document.addEventListener('xprs-app-loaded', extraAppOptions);
+
−
//console.log('listeners added');
+
−
}
+
−
 
+
−
function locationSelected(selVal) {
+
−
if (selVal == 'fullscreen') {
+
 
 
−
popupWidth=window.innerWidth;
+
<li class="removable-parent">
−
popupHeight=window.innerHeight;
+
−
 
+
−
$("[name=popupWidth]").css('display','none');
+
−
$("[name=popupHeight]").css('display','none');
+
−
 
+
−
$("[name=popupWidth]").val(popupWidth);
+
−
$("[name=popupHeight]").val(popupHeight);
+
 
 
−
} else if (selVal == 'bottom_right' || selVal == 'center') {
+
<a class="removable-parent" href="improve.html" data-link-type="EXISTING"  target="_self" >
 
 
−
if (selVal == 'center') {
+
<span id="d9b23d12"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Improve</span>
−
popupWidth=1200;
+
</a>
−
popupHeight=600;
+
−
}
+
</li>
 +
 
 +
 
 
−
if (selVal == 'bottom_right') {
+
<li class="removable-parent">
−
popupWidth=400;
+
−
popupHeight=600;
+
−
}
+
 
 
−
$("[name=popupWidth]").val(popupWidth);
+
<a class="removable-parent" href="results.html" data-link-type="EXISTING"  target="_self" >
−
$("[name=popupHeight]").val(popupHeight);
+
 
 
−
$("[name=popupWidth]").css('display','inline-block');
+
<span id="7e0ed437"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Results</span>
−
$("[name=popupHeight]").css('display','inline-block');
+
</a>
−
}
+
 
 
−
$(".shown #update-html").trigger( "click" );
+
</li>
−
}
+
  
−
function fillPopupList() {
 
−
/*
 
−
var popupSiteVbid = 'vbid-stripe-popup-form-site';//'vbid-09eff374-npdbqrgi'
 
 
 
−
XPRSHelper.GET("/get_box", {"vbid":popupSiteVbid,"no_style":true},function(jsonObj) {
 
 
 
−
boxChildren = jsonObj['CHILDREN'];
+
<li class="removable-parent">
 
 
−
if (boxChildren){
+
<a class="removable-parent" href="attribution.html" data-link-type="EXISTING"   target="_self" >
−
            for idx, entry in enumerate(boxChildren):
+
−
                entryType = str(entry.keys()[0])
+
−
                if entryType == "CHILD":
+
−
                if "TYPE" in entry["CHILD"] and entry["CHILD"]["TYPE"] == "FORM"
+
 
 
−
console.log(jsonObj['CHILDREN']);
+
<span id="8a69633b"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Attribution</span>
−
}
+
</a>
−
});
+
−
*/
+
−
}
+
−
 
+
−
 
+
−
function templateSelected(selVal) {
+
−
 
+
−
//var popupStripeVbid = 'vbid-stripe-popup-form-' + selVal;//+ $(this).prop('id');
+
−
 
+
−
var displayedPanel = $('.shown #app_settings').find('#selectTemplateButton span');
+
−
displayedPanel.addClass("loading-state");
+
−
 
+
−
closeStripe();
+
−
duplicateStripe(selVal, displayedPanel);
+
−
}
+
−
 
+
−
function extraAppOptions() {
+
 
 
−
//extra options display
+
</li>
−
if (popingOptions.indexOf("timer") != -1) {
+
−
$('#app_settings .extra_options.timer').css('display','block');
+
−
} else {
+
−
$('#app_settings .extra_options.timer').css('display','none');
+
−
}
+
−
+
−
if (popingOptions.indexOf("on_exit") != -1) {
+
−
$('#app_settings .extra_options.on_exit').css('display','block');
+
−
} else {
+
−
$('#app_settings .extra_options.on_exit').css('display','none');
+
−
}
+
−
+
−
if (popingOptions.indexOf("on_scroll") != -1) {
+
−
$('#app_settings .extra_options.on_scroll').css('display','block');
+
−
} else {
+
−
$('#app_settings .extra_options.on_scroll').css('display','none');
+
−
}
+
−
+
−
+
−
$("input.app_field_input[type=checkbox]").unbind("click").bind("click",function(){
+
−
+
−
var checkboxId = $(this).attr('id');
+
  
−
if ($('#app_settings .extra_options.'+checkboxId).css('display') == 'none') {
 
−
$('#app_settings .extra_options.'+checkboxId).css('display','block');
 
−
} else {
 
−
$('#app_settings .extra_options.'+checkboxId).css('display','none');
 
−
}
 
−
});
 
 
 
−
var submitBtn = $(".stripe_popup_app a[data-link-type=SUBMIT]");
 
−
 
−
var newText = $('.shown textarea[name=data_text]').val();
 
−
var btnText = submitBtn.attr("data-text");
 
−
 
−
var newEmail = $('.shown input[name=data_href]').val();
 
−
var btnEmail = submitBtn.attr("data-href");
 
−
 
−
 
−
if ((typeof newText != "undefined" && newText != "") && (typeof newEmail != "undefined" && newEmail != "")) {
 
−
 
−
if (newText != btnText || newEmail != btnEmail) {
 
 
 
−
var target,nofollow;
+
<li class="removable-parent">
−
+
−
var item = $(".stripe_popup_app[data-preset-type-id=FORM]");
+
<a class="removable-parent" href="notebook-october.html" data-link-type="EXISTING"  target="_self" >
−
var clickedElement = submitBtn.find("span");
+
−
+
<span id="643d5085" class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook October</span>
−
if (item.length > 0 && clickedElement.length > 0) {
+
</a>
−
+
−
+
−
//setTimeout(function(){
+
−
EditorCore.setElementLink(clickedElement.attr("id"), item.attr("id"), "SUBMIT",newEmail,target,nofollow,newText);
+
−
EditorHelper.wrapElementWithLink(clickedElement,newEmail,"SUBMIT",newText);
+
−
//},1000);
+
−
}
+
−
}
+
−
}
+
 
 
−
document.removeEventListener('xprs-app-loaded', extraAppOptions);
+
</li>
−
}
+
  
−
/*
 
−
function afterUpdateAppOptions () {
 
−
console.log('after update...');
 
−
var newText =
 
−
console.log(data_text);
 
−
$(".stripe_popup_app_animate a[data-link-type='SUBMIT']").attr("data-text",data_text);
 
−
}
 
−
*/
 
−
 
−
function popUpStripe() {
 
−
 
−
if(gDidPop) return;
 
−
 
−
fillPopupList();
 
−
 
−
stripeObj = $('.stripe_popup_app_hide');
 
−
 
−
if(stripeObj.length == 0) {
 
−
stripeObj = $('.stripe_popup_app');
 
−
}
 
−
 
−
backgroundObj = $('#stripe_popup_app_bg');
 
−
 
−
addCloseButtonToStripe(stripeObj);
 
−
 
−
 
−
if (locationOption =="bottom_right") {
 
−
stripeObj.addClass("bottom-right");
 
−
} else {
 
−
stripeObj.removeClass("bottom-right");
 
−
}
 
−
 
−
stripeObj.removeClass("stripe_popup_app_hide");
 
−
stripeObj.addClass("stripe_popup_app");
 
−
stripeObj.addClass("stripe_popup_app_invisible");
 
−
 
−
stripeObj.css({"width":popupWidth+"px","min-height":popupHeight+"px"});
 
−
SpimeEngine.ArrangeHolder(stripeObj);
 
−
 
−
$('body').addClass('popup-mode');
 
−
 
−
stripeObj.removeClass("stripe_popup_app_invisible");
 
−
stripeObj.addClass("stripe_popup_app_animate");
 
−
 
−
stripeObj.removeClass("shadowed");
 
 
 
−
if (locationOption == 'bottom_right') {
 
 
 
−
stripeObj.addClass("shadowed");
+
<li class="removable-parent">
−
backgroundObj.css( 'pointer-events', 'none' );
+
−
 
+
−
} else {
+
 
 
−
backgroundObj.css( 'pointer-events', 'inherit' );
+
<a class="removable-parent" href="silver-hp.html" data-link-type="EXISTING"  target="_self" >
 
 
−
backgroundObj.removeClass("stripe_popup_app_invisible");
+
<span id="element-563894f97a8ebaf" class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Silver HP</span>
−
backgroundObj.addClass("stripe_popup_app_animate");
+
</a>
 
 
−
backgroundObj.unbind("click").bind("click",function (e) {
+
</li>
−
e.stopPropagation();
+
−
closeStripe();
+
−
});
+
−
}
+
−
+
−
if($('#xprs').hasClass('phone-mode')){
+
−
stripeObj.css({'min-height':window.innerHeight - 50 +'px','width':window.innerWidth - 50+'px','height':window.innerHeight - 50 +'px','overflow-y':'auto'});
+
−
stripeObj.removeClass("buttom-right");
+
−
stripeObj.find('.item-wrapper').css("height","auto");
+
−
SpimeEngine.ArrangeHolder(stripeObj);
+
−
}
+
−
+
−
gDidPop = true;
+
−
+
−
document.removeEventListener('xprs-app-loaded', popUpStripe);
+
−
}
+
  
−
function closeStripe() {
 
 
 
−
stripeObj = $('.stripe_popup_app_hide');
 
−
 
−
if(stripeObj.length == 0) {
 
−
stripeObj = $('.stripe_popup_app');
 
−
}
 
−
 
−
backgroundObj = $('#stripe_popup_app_bg');
 
−
 
−
backgroundObj.removeClass("stripe_popup_app_animate");
 
−
backgroundObj.addClass("stripe_popup_app_invisible");
 
−
stripeObj.removeClass("stripe_popup_app_animate");
 
−
stripeObj.addClass("stripe_popup_app_invisible");
 
−
 
−
$('body').removeClass('popup-mode');
 
−
stripeObj.addClass("stripe_popup_app_hide");
 
−
 
−
}
 
−
 
−
function addCloseButtonToStripe(stripeObj) {
 
−
//console.log('add x button');
 
−
var btnColor = "black";
 
−
var closeButton = $("<div class='clickable' style='position: absolute;padding: 20px;right: 0px;top: 0px;width: 20px;height: 20px;color: "+btnColor+";z-index: 999999999999;font-family: Raleway;text-shadow: 1px 1px white;'>X</div>");
 
−
 
−
closeButton.unbind("click").bind("click",function () {
 
−
closeStripe();
 
−
});
 
−
 
−
stripeObj.prepend(closeButton);
 
−
 
−
}
 
−
 
−
function duplicateStripe (stripeVbid, displayedPanel) {
 
−
 
−
var currentPageId = EditorHelper.getMasterId();
 
−
var appKind = "stripe_popup_app";
 
−
 
−
//removing old stripe
 
−
var stripeObj = $("."+appKind+"_hide[data-preset-type-id='FORM'],."+appKind+"_animate[data-preset-type-id='FORM']");
 
−
var stripeId = "";
 
−
 
−
if(stripeObj.length > 0) {
 
−
stripeObj.each(function(){
 
−
var stripeFormId = $(this).attr("id");
 
−
EditorHelper.handleDelete({"vbid":stripeFormId});
 
 
 
−
stripeId = stripeId + "|" + stripeFormId;
+
<li class="removable-parent">
−
});
+
−
} else {
+
−
console.log('no stripe found');
+
−
}
+
−
 
+
−
XPRSHelper.SAFEPOST("/remove_child",{"parent":currentPageId , "child": stripeId,"stripe_id":stripeId},currentPageId,"REMOVE-CHILD",function() {
+
 
 
−
var currentPageId = EditorHelper.getMasterId();
+
<a class="removable-parent" href="integrated-and-gold.html" data-link-type="EXISTING"  target="_self" >
 
 
−
var originalStripeId = stripeVbid;//'vbid-034631be-u8eh7xv3';
+
<span id="element-874635e92416b44" class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Integrated and Gold</span>
−
var originalparentVbid = 'vbid-09eff374-npdbqrgi';//'vbid-stripe-popup-form-site';//'vbid-3a092-7n0wntqn';
+
</a>
−
+
−
$("#empty-stripe").remove();
+
−
var addedHolder = $("<div />").addClass("master item-box animated-height").attr("id","empty-stripe");
+
−
$("#children").append(addedHolder);
+
−
+
−
EditorHelper.safeCloneStripe(originalStripeId,addedHolder,"FORM",currentPageId,originalparentVbid,"last",false, function(){
+
−
 
+
−
addedHolder.addClass("stripe_popup_app_hide");
+
−
 
+
−
SpimeEngine.loadHighResImage($(".stripe_popup_app_hide .load-high-res").not("#no-image"),1600);
+
−
 
+
−
newStripeID = addedHolder.attr("id");
+
−
XPRSHelper.SAFEPOST("/update_css_class", {"vbid":newStripeID,"updated_key":"APP_CLASS","updated_value":"stripe_popup_app_hide","stripe_id":newStripeID}, newStripeID, "CSS_CLASS");
+
−
$(".shown #update-html").trigger( "click" );
+
−
displayedPanel.removeClass("loading-state");
+
−
SpimeEngine.sendVideoCommand(addedHolder.find(".vid-autoplay").attr("id"),"play");
+
−
setTimeout(function(){EditorHelper.askAboutWebsiteStyle(addedHolder, true);
+
−
},2000);
+
−
+
−
}, false);
+
−
});
+
−
 
+
−
}
+
−
 
+
−
 
+
−
//scroll popup code
+
−
 
+
−
function popupStripeAppOnScroll() {
+
 
 
−
if (popingOptions.indexOf("on_scroll") != -1) {
+
</li>
−
    if ( document.documentElement.clientHeight + $(document).scrollTop() >= (document.body.offsetHeight * (endPrecent/100)) ){
+
−
    popUpStripe();
+
−
    }
+
−
}
+
−
}
+
  
−
/// exit popup code
 
  
−
(function(root, factory) {
+
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
−
  if (typeof define === 'function' && define.amd) {
+
−
    define(factory);
+
−
  } else if (typeof exports === 'object') {
+
−
    module.exports = factory(require,exports,module);
+
−
  } else {
+
−
    root.ouibounce = factory();
+
−
  }
+
−
}(this, function(require,exports,module) {
+
−
 
+
−
return function ouibounce(el, custom_config) {
+
−
  "use strict";
+
−
 
+
−
  var config    = custom_config || {},
+
−
    aggressive  = config.aggressive || false,
+
−
    sensitivity  = setDefault(config.sensitivity, 15),
+
−
    timer        = setDefault(config.timer, 1000),
+
−
    delay        = setDefault(config.delay, 0),
+
−
    callback    = config.callback || function() {},
+
−
    cookieExpire = setDefaultCookieExpire(config.cookieExpire) || '',
+
−
    cookieDomain = config.cookieDomain ? ';domain=' + config.cookieDomain : '',
+
−
    cookieName  = config.cookieName ? config.cookieName : 'viewedOuibounceModal',
+
−
    sitewide    = config.sitewide === true ? ';path=/' : '',
+
−
    _delayTimer  = null,
+
−
    _html        = document.documentElement;
+
−
 
+
−
  function setDefault(_property, _default) {
+
−
    return typeof _property === 'undefined' ? _default : _property;
+
−
  }
+
−
 
+
−
  function setDefaultCookieExpire(days) {
+
−
    // transform days to milliseconds
+
−
    var ms = days*24*60*60*1000;
+
−
 
+
−
    var date = new Date();
+
−
    date.setTime(date.getTime() + ms);
+
−
 
+
−
    return "; expires=" + date.toUTCString();
+
−
  }
+
−
 
+
−
  setTimeout(attachOuiBounce, timer);
+
−
  function attachOuiBounce() {
+
−
    if (isDisabled()) { return; }
+
−
 
+
−
    _html.addEventListener('mouseleave', handleMouseleave);
+
−
    _html.addEventListener('mouseenter', handleMouseenter);
+
−
    _html.addEventListener('keydown', handleKeydown);
+
−
  }
+
−
 
+
−
  function handleMouseleave(e) {
+
−
    if (e.clientY > sensitivity) { return; }
+
−
 
+
−
    _delayTimer = setTimeout(fire, delay);
+
−
  }
+
−
 
+
−
  function handleMouseenter() {
+
−
    if (_delayTimer) {
+
−
      clearTimeout(_delayTimer);
+
−
      _delayTimer = null;
+
−
    }
+
−
  }
+
−
 
+
−
  var disableKeydown = false;
+
−
  function handleKeydown(e) {
+
−
    if (disableKeydown) { return; }
+
−
    else if(!e.metaKey || e.keyCode !== 76) { return; }
+
−
 
+
−
    disableKeydown = true;
+
−
    _delayTimer = setTimeout(fire, delay);
+
−
  }
+
−
 
+
−
  function checkCookieValue(cookieName, value) {
+
−
    return parseCookies()[cookieName] === value;
+
−
  }
+
−
 
+
−
  function parseCookies() {
+
−
    // cookies are separated by '; '
+
−
    var cookies = document.cookie.split('; ');
+
−
 
+
−
    var ret = {};
+
−
    for (var i = cookies.length - 1; i >= 0; i--) {
+
−
      var el = cookies[i].split('=');
+
−
      ret[el[0]] = el[1];
+
−
    }
+
−
    return ret;
+
−
  }
+
−
 
+
−
  function isDisabled() {
+
−
    return checkCookieValue(cookieName, 'true') && !aggressive;
+
−
  }
+
−
 
+
−
  // You can use ouibounce without passing an element
+
−
  // https://github.com/carlsednaoui/ouibounce/issues/30
+
−
  function fire() {
+
−
    if (isDisabled()) { return; }
+
−
 
+
−
    if (el) { el.style.display = 'block'; }
+
 
 
−
    popUpStripe();
+
</ul>
−
   
+
</div>
−
    callback();
+
−
    disable();
+
−
  }
+
−
 
+
−
  function disable(custom_options) {
+
−
    var options = custom_options || {};
+
−
 
+
−
    // you can pass a specific cookie expiration when using the OuiBounce API
+
−
    // ex: _ouiBounce.disable({ cookieExpire: 5 });
+
−
    if (typeof options.cookieExpire !== 'undefined') {
+
−
      cookieExpire = setDefaultCookieExpire(options.cookieExpire);
+
−
    }
+
−
 
+
−
    // you can pass use sitewide cookies too
+
−
    // ex: _ouiBounce.disable({ cookieExpire: 5, sitewide: true });
+
−
    if (options.sitewide === true) {
+
−
      sitewide = ';path=/';
+
−
    }
+
−
 
+
−
    // you can pass a domain string when the cookie should be read subdomain-wise
+
−
    // ex: _ouiBounce.disable({ cookieDomain: '.example.com' });
+
−
    if (typeof options.cookieDomain !== 'undefined') {
+
−
      cookieDomain = ';domain=' + options.cookieDomain;
+
−
    }
+
−
 
+
−
    if (typeof options.cookieName !== 'undefined') {
+
−
      cookieName = options.cookieName;
+
−
    }
+
−
 
+
−
    document.cookie = cookieName + '=true' + cookieExpire + cookieDomain + sitewide;
+
−
 
+
−
    // remove listeners
+
−
    _html.removeEventListener('mouseleave', handleMouseleave);
+
−
    _html.removeEventListener('mouseenter', handleMouseenter);
+
−
    _html.removeEventListener('keydown', handleKeydown);
+
−
  }
+
−
 
+
−
  return {
+
−
    fire: fire,
+
−
    disable: disable,
+
−
    isDisabled: isDisabled
+
−
  };
+
−
}
+
−
 
+
−
/*exported ouibounce */
+
−
;
+
−
 
+
−
}));
+
−
 
+
−
 
+
−
if (popingOptions.indexOf("on_exit") != -1) {
+
−
ouibounce(null,{/*<{*/aggressive/*}*/ : /*{*/true/*}>*/});
+
−
}
+
−
 
+
−
//var modal = ouibounce($('#ouibounce-modal')[0],  { /*<{*/aggressive/*}*/ : /*{*/true/*}>*/ });
+
−
/// exit popup code
+
−
 
+
−
</script>
+
−
 
+
−
<style>
+
−
 
+
−
    .stripe_popup_app_bg {
+
−
 
+
−
        position: fixed;
+
−
        top: 0%;
+
−
        left: 0%;
+
−
        width: 100%;
+
−
        height: 100%;
+
−
        background-color: black;
+
−
        z-index:99999991;
+
−
        -moz-opacity: 0.8;
+
−
        opacity:.80;
+
−
        filter: alpha(opacity=80);
+
−
    }
+
−
    .item-box.stripe_popup_app {
+
−
 
+
−
        position: fixed;
+
−
        top: 50%;
+
−
    left: 50%;
+
−
    transform: translateY(-50%) translateX(-50%);
+
−
        z-index:99999992;
+
−
    }
+
−
   
+
−
    #xprs:not(.in-editor) .stripe_popup_app_bg {
+
−
        z-index:9999999991;
+
−
    }
+
−
   
+
−
    #xprs:not(.in-editor) .stripe_popup_app {
+
−
        z-index:9999999992;
+
−
    }
+
−
   
+
−
    .item-box.stripe_popup_app.bottom-right {
+
−
        bottom: 0;
+
−
    right: 0;
+
−
    top: inherit;
+
−
    left: inherit;
+
−
    transform: inherit;
+
−
    }
+
−
   
+
−
    .stripe_popup_app_animate {
+
−
    -webkit-transition: opacity 0.5s 0s, visibility 0s 0s;
+
−
    -moz-transition: opacity 0.5s 0s, visibility 0s 0s;
+
−
    transition: opacity 0.5s 0s, visibility 0s 0s;
+
−
    }
+
−
   
+
−
    .stripe_popup_app_invisible {
+
−
        opacity: 0;
+
−
        height:0px;
+
−
        width:0px;
+
−
        pointer-events:none;
+
−
    }
+
−
   
+
−
    .short_field {
+
−
    width: 50px;
+
−
    height: 25px;
+
−
    }
+
−
   
+
−
    .long_label {
+
−
    width: 100px;
+
−
    }
+
−
   
+
−
    .short_label {
+
−
    width: 25px;
+
−
    }
+
−
   
+
−
    .app_field_input {
+
−
    line-height: 1em;
+
−
    }
+
−
   
+
−
   
+
−
    .popup-mode .master.item-box:not(.stripe_popup_app_animate) .stripe-magic-circle {
+
−
    display: none;
+
−
    }
+
−
   
+
−
    .popup-mode .stripe_popup_app_animate .menu-options .item-settings-menu:not(.settings-btn) {
+
−
    display: none;
+
−
    }
+
−
 
+
−
   
+
−
    .raw-parameters #update-html {
+
−
        margin-left: 15px;
+
−
    }
+
−
   
+
−
    .raw-parameters.switching-panel{
+
−
        margin-left: 0px!important;
+
−
        height: 450px!important;
+
−
    }
+
−
   
+
−
    #xprs-class-selector-holder .raw-parameters.switching-panel {
+
−
        margin-top: 0px;
+
−
    }
+
−
   
+
−
    #app_settings {
+
−
        height: 100%;
+
−
        padding-right: 1.6em;
+
−
        padding-left: 1.6em;
+
−
    }
+
−
   
+
−
    #template_selector {
+
−
    padding-top: 11px;
+
−
    margin-bottom: 8px;
+
−
    background-color: rgba(187, 187, 187, 0.2);
+
−
    height: 37px;
+
−
    /*-webkit-border-radius: 50px;
+
−
    -moz-border-radius: 50px;
+
−
    border-radius: 7px;*/
+
−
    }
+
−
   
+
−
 
+
−
</style>
+
−
 
+
−
 
+
−
<div id="stripe_popup_app_bg" class="stripe_popup_app_bg stripe_popup_app_invisible"></div>
+
−
 
+
−
<!-- special dropdown controller -->
+
−
 
+
−
<style>
+
−
  #feedback { font-size: 1.4em; }
+
−
 
+
−
  #selectable { list-style-type: none; margin: 0; padding: 0; width: 88.3%; overflow: hidden; overflow-x: hidden; top: 54px; height: 0px; position: absolute; top:10px; background-color: #4e4e4e;}
+
−
  #selectable li {padding: 0.4em; font-size: 1.4em; height: 100px; background-color:#4e4e4e; color:white; border:none}
+
−
  #selectable li.item:hover {outline: 12px solid gray;}
+
−
  #selectable li.item{background-size: cover;background-repeat: no-repeat;padding: 0px;margin: 12px;}
+
−
  #selectable li.item.ui-selected {outline: 12px solid #F39814;}
+
−
 
+
−
  #selectable::-webkit-scrollbar-track{background-color: #000000;}
+
−
  #selectable::-webkit-scrollbar {width: 6px; background-color: #777;}
+
−
  #selectable::-webkit-scrollbar-thumb{background-color: #000000;}
+
−
  #selectable::-webkit-scrollbar-corner{background-color: #4e4e4e;}
+
−
  #selectable::-webkit-scrollbar-track-piece{background-color: #4e4e4e;}
+
−
 
+
−
  #selectTemplateButton.template-blank {height:22px;font-size: 10pt;font-family: sans-serif;font-weight: 100;letter-spacing: 0.01em;background: #4e4e4e;margin-top: 6px;margin-left: 75px;}
+
−
  #selectTemplateButton.template-selected {line-height: 38px;background-repeat: no-repeat;height: 37px;margin-top: -6px;margin-left: 5px;margin-right: 5px;background-size: contain;background-position: right;}
+
−
 
+
−
</style>
+
−
+
−
  <script>
+
−
 
+
−
  function expandPopupList() {
+
−
  var selectableTemplates = $( ".shown #selectable" );
+
 
 
−
  /*
+
<button class="hamburger links-menu-btn small" type="button">
−
  $('.raw-edit.shown #xprs-class-selector-holder').bind("click",function(){
+
  <span class="hamburger-box">
−
  console.log('shut it down!');
+
  <span class="hamburger-inner"></span>
−
  $(".shown #selectable").css({"height":"0px","overflow":"hidden"});
+
  </span>
−
  $(this).unbind("click");
+
</button>
−
  });
+
−
  */
+
−
 
+
−
  if(selectableTemplates.find('.ui-selected').length == 0){
+
</div>
−
 
+
−
//var selectedVal = selectableTemplates.attr('value');
+
−
//$( "li[value="+selectedVal+"]" ).addClass('ui-selected');  
+
−
 
+
−
  selectableTemplates.selectable({
+
−
  create:function(event, ui){
+
−
  var selectedVal = selectableTemplates.attr('value');
+
−
  $(event.target).children("li[value="+selectedVal+"]").addClass('ui-selected');
+
−
  },
+
−
      stop:function(event, ui){
+
−
          $(event.target).children('.ui-selected').not(':first').removeClass('ui-selected');
+
−
       
+
−
          var selectedTemplateId = $('.ui-selected').attr('value');
+
−
      var selectedTemplateImage = $('.ui-selected').css('background-image');
+
−
     
+
−
          if(selectedTemplateId) {
+
−
          templateSelected(selectedTemplateId);
+
−
        selectableTemplates.attr('value',selectedTemplateId);
+
−
         
+
−
        setTimeout(function(){
+
−
          selectableTemplates.scrollTop(0);
+
−
          selectableTemplates.css({"height":"0px","overflow":"hidden"});
+
−
         
+
−
          var templatedBtn = $(".shown #selectTemplateButton");
+
−
         
+
−
          templatedBtn.css('background-image',selectedTemplateImage);
+
−
          templatedBtn.removeClass('template-blank');
+
−
          templatedBtn.addClass('template-selected');
+
−
         
+
−
  //trick gilad
+
−
  var disp = selectableTemplates[0].style.display;
+
−
  selectableTemplates[0].style.display = 'none';
+
−
  var trick = selectableTemplates[0].offsetHeight;
+
−
  selectableTemplates[0].style.display = disp;
+
−
 
+
−
         
+
−
},500);
+
−
      }
+
−
      }
+
−
  });
+
−
  }
+
−
 
+
−
  if(selectableTemplates.css("height") == "0px") {
+
−
  selectableTemplates.css({"height":"350px","overflow":"scroll","top":"54px"});
+
−
  } else {
+
−
  selectableTemplates.css({"height":"0px","overflow":"hidden"});
+
−
  }
+
−
 
+
−
  //trick gilad
+
−
  var disp = selectableTemplates[0].style.display;
+
−
  selectableTemplates[0].style.display = 'none';
+
−
  var trick = selectableTemplates[0].offsetHeight;
+
−
  selectableTemplates[0].style.display = disp;
+
−
 
+
−
  }
+
−
  </script>
+
−
 
+
−
 
+
−
 
+
−
+
−
</div>
+
 
</div>
 
</div>
 
</div>
 
</div>
−
+
<!-- LAYOUT SETTINGS -->
−
</div>
+
<div class="layout-settings" style="display:none;" data-type="menu"
 +
data-MENU_OVERLAY="relative"
 +
data-MENU_SCROLL="false"
 +
data-ALWAYS_MINIFIED="false"
 +
data-MENU_POSITION="none"
 +
data-MENU_ALIGN="left"
 +
data-BACKGROUND_COLOR="rgb(124, 100, 41)"
 +
></div>
 +
</div>
 
 
 
</div>
 
</div>
 
</div>
 
</div>
−
+
<!-- MENUS END -->
 
 
 
 
 
 
 
 
−
<!-- ARTICLE START -->
+
<!-- PROMO START -->
−
<div  id="vbid-59a5b-rsrgkesu"  class="master item-box  page-box style-59a5b-mbbodavg         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-59a5b-mbbodavg" data-preview-styleid='style-59a5b-mbbodavg' data-preset-type-id="ARTICLE">
+
<div  id="vbid-04d15a56-prooe7yt"  class="master item-box  gallery-box style-04d15a56-7epp0bbe         " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-04d15a56-7epp0bbe" data-preview-styleid='style-04d15a56-7epp0bbe' data-preset-type-id="PROMO">
 
<div id="no-image" class="stripe-background load-high-res " ></div>
 
<div id="no-image" class="stripe-background load-high-res " ></div>
−
<div class="page-wrapper item-wrapper article-wrapper ">
+
<div class="gallery-wrapper item-wrapper promo-wrapper ">
 
 
−
<div class="item-content leaf blocks_layout page content" data-self="vbid-59a5b-rsrgkesu" data-preview-style="style-59a5b-mbbodavg" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="490" data-orig-thumb-width="490" data-vbid="vbid-59a5b-rsrgkesu" data-bgimg="https://lh3.googleusercontent.com/WUsZbuEMWlGHNmfKl7x69b_5v3NDqJcpqvhR-sRaQVtfjW5cuHrs689zwRnfZw8TocqM2OUzT1MH8TFfzEo">
+
<div class="sub container style-04d15a56-7epp0bbe content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-04d15a56-7epp0bbe" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-04d15a56-prooe7yt" data-preset-type-id="PROMO" data-preview-style="style-04d15a56-7epp0bbe" data-style="style-f3095-j0xhjgzynh" data-absolute-path=""  >
−
<div class="helper-div middle" >
+
<!-- ARRANGER SETTINGS -->
−
<!-- <div class="benet" style="min-height:inherit;"></div> -->
+
<div class="arranger-settings" style="display:none;"
−
<div class="text-side ">
+
data-ARRANGER_TYPE="flex"
−
<div class="vertical-aligner">
+
data-ARRANGER_COLS="0"
−
<div class="item-details blocks-preview-content-wrapper  blocks shrinker-parent" style="position:relative;">
+
data-ARRANGER_ITEM_MAX_WIDTH="400"
−
<div class="blocks-preview-content-holder shrinker-content">
+
data-ARRANGER_ITEM_MIN_WIDTH="230"
−
<!--  BY SPECIFIC ORDER -->
+
data-ARRANGER_ITEM_RATIO="1"
−
+
data-ARRANGER_ITEM_SPACING="20"
−
+
data-ARRANGER_ITEMS_PER_PAGE="all"
−
+
data-ARRANGER_ORDER_TYPE="regular"
−
<br class="upper-line-break" />
+
data-AUTO_PLAY="true"
−
<div class="blocks-preview-title-holder removable-parent order-handle">
+
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
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<h2 id="vbid-59a5b-iarpayyb" class="preview-element blocks-preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="BLOCKS_PREVIEW_TITLE" >Synthetic Biology</h2>
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<p>Synthetic Biology is the shift from understanding and modifying biology to treating biology as an engineering discipline where basic building blocks are assembled from the ground up into larger engineering systems.</p>
 
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<h1 id="vbid-04d15a56-x26nl7vy" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >DESCRIPTION OF OUR PROJECT</h1>
 
 
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<p><span style="color: rgb(48, 48, 48); font-family: Raleway; line-height: 13.2px; text-transform: uppercase;">GET <span style="font-size:22px;"><strong>25%</strong></span> OFF ALL OUR PACKAGES, LEAVE YOUR DETAILES below AND W'LL SEND YOU A COUPON.</span></p>
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<p><span id="docs-internal-guid-1b7da29c-fd54-c3a1-2826-3c55b3c31a0c"><span style="font-size: 12pt; color: rgb(0, 0, 0); background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">The goal of this project is to engineer a biological circuit that utilizes </span><span style="font-size: 12pt; color: rgb(0, 0, 0); background-color: transparent; font-style: italic; vertical-align: baseline; white-space: pre-wrap;">E. coli</span><span style="font-size: 12pt; color: rgb(0, 0, 0); background-color: transparent; vertical-align: baseline; white-space: pre-wrap;"> plasmid DNA sequences and a green fluorescent protein (GFP) reporter to detect arsenic. The constitutive promoter in our construct produces a regulatory protein ArsR that binds with arsenic ions and triggers visible fluorescence. Our team will demonstrate this circuit using a cell-free lateral flow paper-based system. Ultimately, the purpose of this circuit will be to provide an inexpensive and ubiquitous arsenic sensor for testing environmental samples. </span></span>​</p>
 
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<span id="vbid-59a5b-7ptmolat"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >GET CODE</span>
 
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<h2 id="vbid-6c9d2635-qbra6yji" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Goal #1<br></h2>
 
 
 
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<p>Develop tools to work at increasing levels of abstraction and enable facile manufacturing of designs</p>
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<p><span style="font-size:20px;"><span id="docs-internal-guid-1b7da29c-09db-8e3c-11bc-a8b64c92574a"><span style="font-family: Arial; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">Design and create a synthetic biological circuit containing a reporter protein regulated by arsenic ions.</span></span></span></p>
 
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<h2 id="vbid-6c9d2635-ul5d7ano" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Goal #2<br></h2>
 
 
 
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<p>Leverage expertise from established engineering disciplines</p>
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<p><span style="font-size:20px;"><span id="docs-internal-guid-1b7da29c-09dc-2147-201d-0fee97b1058e"><span style="font-family: Arial; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">Demonstrate that the circuit can detect arsenic in solution in cells or using cell-free lysates.</span></span></span></p>
 
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<p>Ultimately, forward engineer novel solutions to diverse problems</p>
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<p><span style="font-size:20px;"><span id="docs-internal-guid-1b7da29c-09dd-066b-7acc-ba488d576ad6"><span style="font-family: Arial; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">Determine the conditions needed for cell-free lysates to detect EPA limits for harmful concentrations of arsenic.</span></span></span></p>
 
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<p>Throughout this project we worked closely with several local non-profits in the Tacoma urban area. The goal of the project was to design a successful biological circuit utilizing e.coli DNA regulatory sequences and a green fluorescent protein (GFP) reporter as a means to detect Arsenic+.</p>
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<p><span style="line-height:1em;"><span style="font-size:16px;"><span style="letter-spacing: 1.1px; white-space: pre-wrap;">Arsenic was selected by Cadets2Vets as the first chemical we would attempt to detect using a biological circuit. It is naturally found in the environment, especially in groundwater, and around industrial plants as a byproduct of manufacturing practices.</span>​</span></span></p><p><br></p><p><span style="line-height:1em;"><span style="font-size:16px;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">The detection of arsenic is especially important to team </span></span><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">members in the Puget Sound area, because North Tacoma is a Superfund Site. The ASARCO smelting company smokestack, erected in 1917, was a symbol of economic prosperity and booming business in the developing city of Tacoma. The copper smelting site on Ruston’s shore in Puget Sound brought jobs and wealth to the area. Unfortunately, over the course of its 100 years of operation, the plant had been pumping toxic materials like arsenic and lead directly into the air, where it then settled into the surrounding soil and water of the greater Tacoma area.</span></span></span></span></p><p><br></p><p><span style="line-height:1em;"><span style="font-size:16px;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">The smokestack was demolished in 1985, but there are still toxic levels of heavy metals in the soil over 30 years later. The city of Tacoma takes measures to warn new Tacoma homeowners about how to mitigate any increased levels of exposure to toxic materials in their soil, but must also take more drastic steps to protect users of publicly-owned land.</span><br class="kix-line-break"><br class="kix-line-break"><span style="vertical-align: baseline; white-space: pre-wrap;">The Department of Defense has a strong interest in developing rapid assays to test for environmental risks to protect the health of service members. Troops often deploy to hostile environments and assessing the risks of the environment itself can be challenging. The development of the arsenic circuit represents a new way to use synthetic biology in the military setting. Demonstrating that a biological circuit can be used in this application will lead to an expansion of the technology to test for more substances in the future. </span></span></span></span></p>
 
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<p>After the circuit was designed and constructed the next step was to demonstrate the circuit as viable in a cell of Arsenic. To accomplish this goal we worked with Urban Waters, Readiness Acceleration and Innovation Networking (RAIN),and Charles Wright Academy.</p>
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<p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">The maximum contaminant level for arsenic that federal and state agencies use as guideline to test the arsenic levels in drinking water was established by the EPA. Initially it was set to 50 micrograms/liter or 50 ppb. However, drinking water that contains 50 ppb arsenic for prolonged periods of time may cause bladder or lung cancer and other chronic conditions.</span></span></span></span><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;"></span></span></span></span><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;"> </span></span></span></span><br></p><p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;"></span></span></span></span><br></p><p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">In 2001, the EPA reduced the maximum contaminant level of arsenic to 10 micrograms/ liter or 10 ppb</span><span style="vertical-align: super; white-space: pre-wrap;">1</span><span style="vertical-align: baseline; white-space: pre-wrap;">. Nevertheless, according to data gathered between the years of 1993 and 2007, arsenic contamination of the waters in Washington state persisted</span><span style="vertical-align: super; white-space: pre-wrap;">2</span><span style="vertical-align: baseline; white-space: pre-wrap;">.</span></span></span></span><br></p><p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;"></span></span></span></span><br></p><p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">Probes in the waters of western part of Washington state, </span></span></span></span><span style="font-size: 16px; letter-spacing: 1.1px; white-space: pre-wrap; background-color: rgb(255, 255, 255);">near the southern part of the state by the Columbia River, and also north near Everett</span><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;"> have detected higher concentrations of arsenic, close to 50 ppb. </span></span></span></span><br></p><p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;"></span></span></span></span><br></p><p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">Developing an inexpensive paper-based circuit to detect the concentration of arsenic in the water in the region will greatly influence our knowledge and awareness of the quality of the water are consuming.</span></span><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">We hope to develop our assay to detect arsenic within the EPA limit.</span></span></span></span></p>
 
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<p>By utilizing community involvement we hope to both increase pre-collegiate interest in STEM related fields as well as increase the communal understanding of the environment and provide a inexpensive and effective tool for the detection of potentially toxic metals.</p>
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<p></p><ol><li><span><span style="font-size: 12pt; font-family: Arial; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">“Drinking Water Arsenic Rule History”, EPA.gov. https://www.epa.gov/dwreginfo/drinking-water-arsenic-rule-history. Web. Sept 06, 2017.</span></span><br></li><li><span><span id="docs-internal-guid-1b7da29c-fdbd-fb12-e772-bbf347097214"><span style="font-size: 12pt; font-family: Arial; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">“Arsenic Detection in Washington State Public Water Supplies”, doh.wa.gov. http://www.doh.wa.gov/CommunityandEnvironment/DrinkingWater/Contaminants/ArsenicinDrinkingWater/MapofArsenicDetections. Web. Sept 06, 2017.</span></span></span></li></ol>
 
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Latest revision as of 16:05, 1 November 2017

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Description - Home


DESCRIPTION OF OUR PROJECT



The goal of this project is to engineer a biological circuit that utilizes E. coli plasmid DNA sequences and a green fluorescent protein (GFP) reporter to detect arsenic. The constitutive promoter in our construct produces a regulatory protein ArsR that binds with arsenic ions and triggers visible fluorescence. Our team will demonstrate this circuit using a cell-free lateral flow paper-based system. Ultimately, the purpose of this circuit will be to provide an inexpensive and ubiquitous arsenic sensor for testing environmental samples. ​



Goal #1



Design and create a synthetic biological circuit containing a reporter protein regulated by arsenic ions.



Goal #2



Demonstrate that the circuit can detect arsenic in solution in cells or using cell-free lysates.



Goal #3



Determine the conditions needed for cell-free lysates to detect EPA limits for harmful concentrations of arsenic.



why we chose arsenic





Arsenic was selected by Cadets2Vets as the first chemical we would attempt to detect using a biological circuit. It is naturally found in the environment, especially in groundwater, and around industrial plants as a byproduct of manufacturing practices.​


The detection of arsenic is especially important to team members in the Puget Sound area, because North Tacoma is a Superfund Site. The ASARCO smelting company smokestack, erected in 1917, was a symbol of economic prosperity and booming business in the developing city of Tacoma. The copper smelting site on Ruston’s shore in Puget Sound brought jobs and wealth to the area. Unfortunately, over the course of its 100 years of operation, the plant had been pumping toxic materials like arsenic and lead directly into the air, where it then settled into the surrounding soil and water of the greater Tacoma area.


The smokestack was demolished in 1985, but there are still toxic levels of heavy metals in the soil over 30 years later. The city of Tacoma takes measures to warn new Tacoma homeowners about how to mitigate any increased levels of exposure to toxic materials in their soil, but must also take more drastic steps to protect users of publicly-owned land.

The Department of Defense has a strong interest in developing rapid assays to test for environmental risks to protect the health of service members. Troops often deploy to hostile environments and assessing the risks of the environment itself can be challenging. The development of the arsenic circuit represents a new way to use synthetic biology in the military setting. Demonstrating that a biological circuit can be used in this application will lead to an expansion of the technology to test for more substances in the future.



arsenic levels of interest





The maximum contaminant level for arsenic that federal and state agencies use as guideline to test the arsenic levels in drinking water was established by the EPA. Initially it was set to 50 micrograms/liter or 50 ppb. However, drinking water that contains 50 ppb arsenic for prolonged periods of time may cause bladder or lung cancer and other chronic conditions.


In 2001, the EPA reduced the maximum contaminant level of arsenic to 10 micrograms/ liter or 10 ppb1. Nevertheless, according to data gathered between the years of 1993 and 2007, arsenic contamination of the waters in Washington state persisted2.


Probes in the waters of western part of Washington state, near the southern part of the state by the Columbia River, and also north near Everett have detected higher concentrations of arsenic, close to 50 ppb.


Developing an inexpensive paper-based circuit to detect the concentration of arsenic in the water in the region will greatly influence our knowledge and awareness of the quality of the water are consuming.We hope to develop our assay to detect arsenic within the EPA limit.



Sources



  1. “Drinking Water Arsenic Rule History”, EPA.gov. https://www.epa.gov/dwreginfo/drinking-water-arsenic-rule-history. Web. Sept 06, 2017.
  2. “Arsenic Detection in Washington State Public Water Supplies”, doh.wa.gov. http://www.doh.wa.gov/CommunityandEnvironment/DrinkingWater/Contaminants/ArsenicinDrinkingWater/MapofArsenicDetections. Web. Sept 06, 2017.

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