Difference between revisions of "Team:Cadets2Vets/Description"

Arsenic was selected by Cadets2Vets as the first chemical we would attempt to detect using a biological circuit. It is naturally found in the environment, especially in groundwater, and around industrial plants as a byproduct of manufacturing practices.

The detection of arsenic is especially important to team members in the Puget Sound area, because North Tacoma is a Superfund Site. The ASARCO smelting company smokestack, erected in 1917, was a symbol of economic prosperity and booming business in the developing city of Tacoma. The copper smelting site on Ruston’s shore in Puget Sound brought jobs and wealth to the area. Unfortunately, over the course of its 100 years of operation, the plant had been pumping toxic materials like arsenic and lead directly into the air, where it then settled into the surrounding soil and water of the greater Tacoma area.

The smokestack was demolished in 1985, but there are still toxic levels of heavy metals in the soil over 30 years later. The city of Tacoma takes measures to warn new Tacoma homeowners about how to mitigate any increased levels of exposure to toxic materials in their soil, but must also take more drastic steps to protect users of publicly-owned land.

The Department of Defense has a strong interest in developing rapid assays to test for environmental risks to protect the health of service members. Troops often deploy to hostile environments and assessing the risks of the environment itself can be challenging. The development of the arsenic circuit represents a new way to use synthetic biology in the military setting. Demonstrating that a biological circuit can be used in this application will lead to an expansion of the technology to test for more substances in the future.

The maximum contaminant level for arsenic that federal and state agencies use as guideline to test the arsenic levels in drinking water was established by the EPA. Initially it was set to 50 micrograms/liter or 50 ppb. However, drinking water that contains 50 ppb arsenic for prolonged periods of time may cause bladder or lung cancer and other chronic conditions.

In 2001, the EPA reduced the maximum contaminant level of arsenic to 10 micrograms/ liter or 10 ppb1. Nevertheless, according to data gathered between the years of 1993 and 2007, arsenic contamination of the waters in Washington state persisted2.

Probes in the waters of western part of Washington state, near the southern part of the state by the Columbia River, and also north near Everett have detected higher concentrations of arsenic, close to 50 ppb.

Developing an inexpensive paper-based circuit to detect the concentration of arsenic in the water in the region will greatly influence our knowledge and awareness of the quality of the water are consuming.We hope to develop our assay to detect arsenic within the EPA limit.

Difference between revisions of "Team:Cadets2Vets/Description"

Latest revision as of 16:05, 1 November 2017

DESCRIPTION OF OUR PROJECT

Goal #1

Goal #2

Goal #3

why we chose arsenic

arsenic levels of interest

Sources

Follow us on Social Media!

2304 Jefferson Ave, Tacoma, WA 98402

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+			<a class="removable-parent" href="notebook-july.html" data-link-type="EXISTING"   target="_self" >
+			<span id="element-09ca318ecaf1b2f"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook July</span>
+			</a>
+		</li>
+		<li class="removable-parent">
+			<a class="removable-parent" href="notebook-june.html" data-link-type="EXISTING"   target="_self" >
+			<span id="element-367041e6445b247"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook June</span>
+			</a>
+		</li>
+		<li class="removable-parent">
+			<a class="removable-parent" href="public-engagement.html" data-link-type="EXISTING"   target="_self" >
+			<span id="element-307cb61a9a844e1"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Public Engagement</span>
+			</a>
+		</li>
+		<li class="removable-parent">
+			<a class="removable-parent" href="safety.html" data-link-type="EXISTING"   target="_self" >
+			<span id="element-fa38138e1dd4fce"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Safety</span>
+			</a>
+		</li>
+		<li class="removable-parent">
+			<a class="removable-parent" href="team.html" data-link-type="EXISTING"   target="_self" >
+			<span id="element-8308751370782bc"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Team</span>
+			</a>
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+			<a class="removable-parent" href="basic-parts.html" data-link-type="EXISTING"   target="_self" >
+			<span id="element-78fbd0423085ce2"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Basic Parts</span>
+			</a>
+		</li>
+		<li class="removable-parent">
+			<a class="removable-parent" href="composite-parts.html" data-link-type="EXISTING"   target="_self" >
+			<span id="element-24ea54f35798e9a"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Composite Parts</span>
+			</a>
+		</li>
+		<li class="removable-parent">
+			<a class="removable-parent" href="parts.html" data-link-type="EXISTING"   target="_self" >
+			<span id="element-8d68d4dbac38a10"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Parts</span>
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+			<a class="removable-parent" href="interlab.html" data-link-type="EXISTING"   target="_self" >
+			<span id="element-c92600692c09125"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Interlab</span>
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+			<span id="element-be35f8069be1925"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Our Partners</span>
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+			<a class="removable-parent" href="notebook-september.html" data-link-type="EXISTING"   target="_self" >
+			<span id="element-4d7dd9f5c90ef9b"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook September</span>
+			</a>
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+		<li class="removable-parent">
+			<a class="removable-parent" href="environmental-project.html" data-link-type="EXISTING"   target="_self" >
+			<span id="2d522622"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Environmental Project</span>
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+			<span id="8d3cfa8c"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Hardware</span>
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+		</li>
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+			<span id="1ad7c153"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Demonstrate</span>
+			</a>
+		</li>
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+			<span id="d9b23d12"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Improve</span>
+			</a>
+		</li>
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+			<a class="removable-parent" href="results.html" data-link-type="EXISTING"   target="_self" >
+			<span id="7e0ed437"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Results</span>
+			</a>
+		</li>
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+			<span id="8a69633b"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Attribution</span>
+			</a>
+		</li>
+		<li class="removable-parent">
+			<a class="removable-parent" href="notebook-october.html" data-link-type="EXISTING"   target="_self" >
+			<span id="643d5085"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Notebook October</span>
+			</a>
+		</li>
+		<li class="removable-parent">
+			<a class="removable-parent" href="silver-hp.html" data-link-type="EXISTING"   target="_self" >
+			<span id="element-563894f97a8ebaf"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Silver HP</span>
+			</a>
+		</li>
+		<li class="removable-parent">
+			<a class="removable-parent" href="integrated-and-gold.html" data-link-type="EXISTING"   target="_self" >
+			<span id="element-874635e92416b44"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Integrated and Gold</span>
 			</a>
@@ Line 253: / Line 459: @@
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 			<div id="no-image" class="stripe-background load-high-res " ></div>
-			<div class="page-wrapper item-wrapper form-wrapper ">
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 <div  class="multi-container preview image-cover" >
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@@ Line 268: / Line 503: @@
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@@ Line 299: / Line 534: @@
 									<!--  BY SPECIFIC ORDER -->
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 <div class="preview-title-holder removable-parent order-handle">
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 </div>
@@ Line 350: / Line 555: @@
 <div class="preview-body-holder removable-parent order-handle">
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+	<div id="vbid-04d15a56-zlmqmubp" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_BODY">
-		<p style="text-align: center;">- 2017 iGEM Team -</p>
+		<p><span id="docs-internal-guid-1b7da29c-fd54-c3a1-2826-3c55b3c31a0c"><span style="font-size: 12pt; color: rgb(0, 0, 0); background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">The goal of this project is to engineer a biological circuit that utilizes </span><span style="font-size: 12pt; color: rgb(0, 0, 0); background-color: transparent; font-style: italic; vertical-align: baseline; white-space: pre-wrap;">E. coli</span><span style="font-size: 12pt; color: rgb(0, 0, 0); background-color: transparent; vertical-align: baseline; white-space: pre-wrap;"> plasmid DNA sequences and a green fluorescent protein (GFP) reporter to detect arsenic. The constitutive promoter in our construct produces a regulatory protein ArsR that binds with arsenic ions and triggers visible fluorescence. Our team will demonstrate this circuit using a cell-free lateral flow paper-based system. Ultimately, the purpose of this circuit will be to provide an inexpensive and ubiquitous arsenic sensor for testing environmental samples. </span></span></p>
 	</div>
@@ Line 379: / Line 584: @@
 			</div>
 		</div>
-		<!-- FORM END -->
+			</div>
+		</div>
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@@ Line 434: / Line 654: @@
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+<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
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-</div>
 		<!-- MAP TEMPLATE -->
@@ Line 466: / Line 678: @@
 							<div class="preview-content-holder shrinker-content">
-									<!--  BY SPECIFIC ORDER -->
+										<!--  BY NATIVE ORDER -->
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+<br class="upper-line-break" />
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+</div>
+<br class="lower-line-break" />
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+<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
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+		<p><span style="font-size:20px;"><span id="docs-internal-guid-1b7da29c-09db-8e3c-11bc-a8b64c92574a"><span style="font-family: Arial; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">Design and create a synthetic biological circuit containing a reporter protein regulated by arsenic ions.</span></span></span></p>
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 <div class="preview-title-holder removable-parent order-handle">
-	<h2 id="vbid-59a5b-j6hw2jzr" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >WE ARE Cadets2Vets.</h2>
+	<h2 id="vbid-6c9d2635-ul5d7ano" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Goal #2<br></h2>
 </div>
 <br class="lower-line-break" />
+<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 <br class="upper-line-break" />
-<div class="preview-subtitle-holder removable-parent order-handle">
+<div class="preview-body-holder removable-parent order-handle">
+	<div id="vbid-6c9d2635-0atjkqde" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_BODY">
+		<p><span style="font-size:20px;"><span id="docs-internal-guid-1b7da29c-09dc-2147-201d-0fee97b1058e"><span style="font-family: Arial; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">Demonstrate that the circuit can detect arsenic in solution in cells or using cell-free lysates.</span></span></span></p>
+	</div>
-	<h3 id="vbid-59a5b-potfnoot" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">2304 Jefferson Ave, <br>Tacoma, WA 98402<br></h3>
 </div>
 <br class="lower-line-break" />
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+											<div class="preview-form order-handle">
+<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
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+	<div class="preview-links-wrapper">
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-												<div class="preview-item-links order-handle removable-parent" style="display:inline-block;">
-	<span id="vbid-59a5b-hgd5ta77"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >info@rainincubator.org</span>
+<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
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-			<div class="helper-div middle-left" >
+			<div class="helper-div middle-center" >
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+					<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
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+	<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
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-									<!--  BY SPECIFIC ORDER -->
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+<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
-	<div class="preview-icon-holder Icon removable-parent order-handle" >
-		<img id="vbid-59a5b-x6dz6l6a" class="preview-element icon-source magic-circle-holder shrinkable-img  allow-mobile-hide" data-menu-name="PREVIEW_ICON" src="https://lh3.googleusercontent.com/RV9GQfQJ9BfAg_aSqdCOkSsuOMs7KUIGWbLAgwgxWEQsO3uW297q-3ex471NB0-4m-af1nKLiwPpEUHf" />
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+	<h2 id="vbid-6c9d2635-lns7saw8" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Goal #3<br></h2>
 </div>
 <br class="lower-line-break" />
+<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 <br class="upper-line-break" />
-<div class="preview-subtitle-holder removable-parent order-handle">
+<div class="preview-body-holder removable-parent order-handle">
+	<div id="vbid-6c9d2635-yxv565h7" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_BODY">
+		<p><span style="font-size:20px;"><span id="docs-internal-guid-1b7da29c-09dd-066b-7acc-ba488d576ad6"><span style="font-family: Arial; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">Determine the conditions needed for cell-free lysates to detect EPA limits for harmful concentrations of arsenic.</span></span></span></p>
+	</div>
-	<h3 id="vbid-59a5b-7isk1yxx" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">2304 Jefferson Ave, <br>Tacoma, WA 98402<br></h3>
 </div>
 <br class="lower-line-break" />
+<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
+											<div class="preview-form order-handle">
+<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
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+	<div class="preview-links-wrapper">
+<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
+	</div>
+</div>
-												<div class="preview-item-links order-handle removable-parent" style="display:inline-block;">
-	<span id="vbid-59a5b-hapd8kb8"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >info@rainincubator.org</span>
+<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
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+		data-ARRANGER_ITEM_SPACING="20"
+		data-ARRANGER_ITEMS_PER_PAGE="all"
+		data-ARRANGER_ORDER_TYPE="regular"
+		data-AUTO_PLAY="true"
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-			<div class="helper-div middle-left" >
+			<div class="helper-div middle-right" >
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 				<div class="vertical-aligner">
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+<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
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-	<div class="preview-icon-holder Icon removable-parent order-handle" >
+<br class="upper-line-break" />
+<div class="preview-title-holder removable-parent order-handle">
-		<img id="vbid-59a5b-fhlvsspa" class="preview-element icon-source magic-circle-holder shrinkable-img  allow-mobile-hide" data-menu-name="PREVIEW_ICON" src="https://lh3.googleusercontent.com/RV9GQfQJ9BfAg_aSqdCOkSsuOMs7KUIGWbLAgwgxWEQsO3uW297q-3ex471NB0-4m-af1nKLiwPpEUHf" />
+	<h2 id="vbid-4c38363c-lhrwnuia" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >why we chose arsenic<br></h2>
-	</div>
+</div>
+<br class="lower-line-break" />
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 <br class="upper-line-break" />
-<div class="preview-title-holder removable-parent order-handle">
+<div class="preview-divider-holder removable-parent order-handle">
+	<div id="vbid-4c38363c-i5dg9lve" class="preview-element preview-divider magic-circle-holder quick-text-style-menu" data-menu-name="PREVIEW_DIVIDER">
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+	</div>
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 <br class="upper-line-break" />
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+		<p><span style="line-height:1em;"><span style="font-size:16px;"><span style="letter-spacing: 1.1px; white-space: pre-wrap;">Arsenic was selected by Cadets2Vets as the first chemical we would attempt to detect using a biological circuit. It is naturally found in the environment, especially in groundwater, and around industrial plants as a byproduct of manufacturing practices.</span></span></span></p><p><br></p><p><span style="line-height:1em;"><span style="font-size:16px;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">The detection of arsenic is especially important to team </span></span><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">members in the Puget Sound area, because North Tacoma is a Superfund Site. The ASARCO smelting company smokestack, erected in 1917, was a symbol of economic prosperity and booming business in the developing city of Tacoma. The copper smelting site on Ruston’s shore in Puget Sound brought jobs and wealth to the area. Unfortunately, over the course of its 100 years of operation, the plant had been pumping toxic materials like arsenic and lead directly into the air, where it then settled into the surrounding soil and water of the greater Tacoma area.</span></span></span></span></p><p><br></p><p><span style="line-height:1em;"><span style="font-size:16px;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">The smokestack was demolished in 1985, but there are still toxic levels of heavy metals in the soil over 30 years later. The city of Tacoma takes measures to warn new Tacoma homeowners about how to mitigate any increased levels of exposure to toxic materials in their soil, but must also take more drastic steps to protect users of publicly-owned land.</span><br class="kix-line-break"><br class="kix-line-break"><span style="vertical-align: baseline; white-space: pre-wrap;">The Department of Defense has a strong interest in developing rapid assays to test for environmental risks to protect the health of service members. Troops often deploy to hostile environments and assessing the risks of the environment itself can be challenging. The development of the arsenic circuit represents a new way to use synthetic biology in the military setting. Demonstrating that a biological circuit can be used in this application will lead to an expansion of the technology to test for more substances in the future. </span></span></span></span></p>
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-	<span id="vbid-59a5b-ji7uyawu"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >info@rainincubator.org</span>
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+		<p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">The maximum contaminant level for arsenic that federal and state agencies use as guideline to test the arsenic levels in drinking water was established by the EPA. Initially it was set to 50 micrograms/liter or 50 ppb. However, drinking water that contains 50 ppb arsenic for prolonged periods of time may cause bladder or lung cancer and other chronic conditions.</span></span></span></span><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;"></span></span></span></span><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;"> </span></span></span></span><br></p><p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;"></span></span></span></span><br></p><p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">In 2001, the EPA reduced the maximum contaminant level of arsenic to 10 micrograms/ liter or 10 ppb</span><span style="vertical-align: super; white-space: pre-wrap;">1</span><span style="vertical-align: baseline; white-space: pre-wrap;">. Nevertheless, according to data gathered between the years of 1993 and 2007, arsenic contamination of the waters in Washington state persisted</span><span style="vertical-align: super; white-space: pre-wrap;">2</span><span style="vertical-align: baseline; white-space: pre-wrap;">.</span></span></span></span><br></p><p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;"></span></span></span></span><br></p><p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">Probes in the waters of western part of Washington state, </span></span></span></span><span style="font-size: 16px; letter-spacing: 1.1px; white-space: pre-wrap; background-color: rgb(255, 255, 255);">near the southern part of the state by the Columbia River, and also north near Everett</span><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;"> have detected higher concentrations of arsenic, close to 50 ppb. </span></span></span></span><br></p><p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;"></span></span></span></span><br></p><p><span style="font-size:16px;"><span style="line-height:1em;"><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">Developing an inexpensive paper-based circuit to detect the concentration of arsenic in the water in the region will greatly influence our knowledge and awareness of the quality of the water are consuming.</span></span><span style="letter-spacing: 1.1px;"><span style="vertical-align: baseline; white-space: pre-wrap;">We hope to develop our assay to detect arsenic within the EPA limit.</span></span></span></span></p>
+	</div>
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-	<span id="vbid-59a5b-e7h4yynk"  class="preview-element Link item-link magic-circle-holder text-element " data-menu-name="PREVIEW_LINK" >info@rainincubator.org</span>
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+		<p></p><ol><li><span><span style="font-size: 12pt; font-family: Arial; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">“Drinking Water Arsenic Rule History”, EPA.gov. https://www.epa.gov/dwreginfo/drinking-water-arsenic-rule-history. Web. Sept 06, 2017.</span></span><br></li><li><span><span id="docs-internal-guid-1b7da29c-fdbd-fb12-e772-bbf347097214"><span style="font-size: 12pt; font-family: Arial; background-color: transparent; vertical-align: baseline; white-space: pre-wrap;">“Arsenic Detection in Washington State Public Water Supplies”, doh.wa.gov. http://www.doh.wa.gov/CommunityandEnvironment/DrinkingWater/Contaminants/ArsenicinDrinkingWater/MapofArsenicDetections. Web. Sept 06, 2017.</span></span></span></li></ol>
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-	<h3 id="element-1b60f7ad57662d8" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">"The purpose of this team is to build an everlasting connection with our future and past leaders in order to foster futuristic thinking and ideas."<br>- Cadets2Vets Team Purpose, iGEM 2017<br></h3>
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