Difference between revisions of "Team:SUIS Alpha Shanghai/InterLab"

 
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       <a class="navname" href="https://2017.igem.org/Team:SUIS_Alpha_Shanghai/InterLab">InterLab</a>
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<div class="top" id="top" style="position:fixed;"><center>Interlab</center></div>
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<h2><i>How close can the numbers be when fluorescence is measured all around the world?.</i></h2>
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<p>We are very proud to be able to contribute to the 2017 intrlab study. For us at SUIS it is first time we participate in iGEM, the interlab study provides us with a way to connect to the wider iGEM and synthetic biology community. The study also provided us with a worthwhile experience through collaborating and building relationships with some science departments at universities within our community. Overall the process was enriching and allowed us as a team to grow in confidence and develop our skills, in preparation for the design of our main project. <br>
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The purpose of this years study is for participating interlab teams to collect data which will be used to establish a Green Florescent Protein (GFP) measurement protocol based on engineering principles that anyone with a plate reader can use in their lab. <br>
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We followed the official standardized protocol from the iGEM foundation. Follow this link to learn more about the protocol. <a href:"https://2017.igem.org/Competition/InterLab_Study">iGEM Interlab Information</a></p>
  
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<p>This has been the first time we participate in igem competition and engage in the interlab study. The purpose of this years study is to figure out the fine difference between the plate readers all around the world. We are excited about the experiment and these are some datas we collected from interlab.</p>
 
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<p>OD600 referencing point</p>
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<center><h1>OD600 referencing point</h1><center>
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<p>Fluorensein Standard Curve</p>
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<h3>Collaboration</h3>
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<center><h1>Fluorensein Standard Curve</h1><center>
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<p>We get in touch with team Light Harvester in June through our instructor. Even though we’ve go though the HighSchool student handbook, we’re still lack of some biotechnology lab skill. And since our lab is specifically established for the igem project in this year, we are lack of equipment resources. As a result, we learned extraction and transformation of plasmids, standardized aseptic manipulation, the extraction and purification of DNA, and how to operate various instruments and so on. With all the knowledges we needed for completing the interlab, our team start the interlab in August 8th. We finished our final measurement of interlab with their assistants about plate reader and their help upon the cultivation of final. In the first week, we dilute the fluorescein and measure the fluorescein solution’s standard curve. In the week after that, we measured OD600 Reference point and fluorescein fluorescence standard curve using protocols. Other than communicate about the experimental design between two groups, our team help them to check english grammar use.</p>
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  <center><span class="ny4nr2">Our team is composed of a group of young and motivated students with helpful advisors from Shanghai United International School Wanyuan Campus. We all love synthetic biology. We aim to make a contribution for the society and improve the food safety issue. </span></center>
 
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Latest revision as of 16:28, 1 November 2017

Inter Lab

INTER LAB
Interlab

How close can the numbers be when fluorescence is measured all around the world?.

We are very proud to be able to contribute to the 2017 intrlab study. For us at SUIS it is first time we participate in iGEM, the interlab study provides us with a way to connect to the wider iGEM and synthetic biology community. The study also provided us with a worthwhile experience through collaborating and building relationships with some science departments at universities within our community. Overall the process was enriching and allowed us as a team to grow in confidence and develop our skills, in preparation for the design of our main project.
The purpose of this years study is for participating interlab teams to collect data which will be used to establish a Green Florescent Protein (GFP) measurement protocol based on engineering principles that anyone with a plate reader can use in their lab.
We followed the official standardized protocol from the iGEM foundation. Follow this link to learn more about the protocol. iGEM Interlab Information

OD600 referencing point



Fluorensein Standard Curve
Powered by SUIS Team, 2017