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We designed two constructs, known as biobricks, to allow the bacteria to express the proteins lipase and esterase that will degrade PET plastics. These constructs are identical except for the sequence for the enzyme each construct would produce: esterase and lipase. Our constructs were designed as follows. | We designed two constructs, known as biobricks, to allow the bacteria to express the proteins lipase and esterase that will degrade PET plastics. These constructs are identical except for the sequence for the enzyme each construct would produce: esterase and lipase. Our constructs were designed as follows. | ||
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<strong>Lactose Regulated Promoter (R0011):</strong> a DNA sequence that starts the transcription process in the presence of lactose or IPTG. Thus, the promoter is only active in certain times to regulate the amount of proteins the bacteria is able to produce at a time. The promoter causes the gene to activate and begin the process of forming mRNA and eventually proteins. | <strong>Lactose Regulated Promoter (R0011):</strong> a DNA sequence that starts the transcription process in the presence of lactose or IPTG. Thus, the promoter is only active in certain times to regulate the amount of proteins the bacteria is able to produce at a time. The promoter causes the gene to activate and begin the process of forming mRNA and eventually proteins. |
Revision as of 16:55, 1 November 2017
![BUGSS Logo](https://static.igem.org/mediawiki/2017/e/e2/Baltimore_biocre_BugssLOGO.png)
BALTIMORE BIO-CREW
Bio-Engineering E.Coli To Degrade Plastic and Save The Baltimore Inner Harbor
COMPOSITE PARTS
![](https://static.igem.org/mediawiki/2017/a/a5/T--Baltimore_Bio-Crew--small_construct_diagrams.png)