Difference between revisions of "Team:INSA-UPS France/Contribution"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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    <section style="display:table;background:none;padding:0px !important;z-index:100; ">
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      <h1 style="vertical-align:bottom;display:table-cell; width:70%;font-size:60pt;letter-spacing: 0.2em;z-index:120;text-align: center;">Parts</h1>
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      <img style="vertical-align:bottom;display:table-cell; width:100%;" src="https://static.igem.org/mediawiki/2017/7/7e/T--INSA-UPS_France--Parts_croco.png" alt="">
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<h1>Contribution</h1>
 
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<h3>Bronze Medal Criterion #4</h3>
 
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study (to be documented on your InterLab page) and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range. Teams who are working on improving the characterization of an existing part should document their experimental design here, along with an explanation for why they chose that part to improve. Data can also be shown here, but it MUST also be documented on the part's Main Page in the Registry.
 
 
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<br><br>
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<b>Special Tracks:</b> Document at least one new substantial contribution to the iGEM community that showcases a project related to BioBricks. This contribution should be central to your project and equivalent in difficulty to making and submitting a BioBrick part.
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<h2> *** All parts listed here are available upon request *** <h/2>
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<p>Feel free to contact us, if you want to know more about our parts:  <a HREF="mailto: igem.toulouse@gmail.com ?subject=Parts iGEM INSA-UPS 2017 request ">igem.toulouse@gmail.com</a></p>
  
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<br><br>
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<br><br>
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    <h1>Existing Parts we have contributed to characterized</h1>
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<br><br>
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  <h2></html><partinfo>BBa_J04450</partinfo><html>: RFP coding device </h2>
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    <figure>
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      <img src="https://static.igem.org/mediawiki/parts/1/14/T--INSA-UPS_France--J04450.png" alt="">
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      <figcaption>
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        Figure 1: BBa_J04450 biobrick conjugated in <i>Vibrio harveyi</i>.
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      </figcaption>
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    </figure>
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    <p>
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      BBa_J04450 was tested in the <i>Vibrio harveyi</i> background. The biobrick was cloned in a broad host range plasmid (pBBR1MCS-4) and conjugated into <i>Vibrio harveyi</i> to demonstrate the production of RFP in this chassis.
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    </p>
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    <p>
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      <b>To learn more:</b> see </html><partinfo>BBa_J04450</partinfo><html> and our <a href="https://2017.igem.org/Team:INSA-UPS_France/Results">results</a>.
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    </p>
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<br><br>
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  <h2></html><partinfo>BBa_K431009</partinfo><html>: glyceraldehyde 3-phosphate dehydrogenase promoter (pGAP)</h2>
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    <figure>
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      <img src="https://static.igem.org/mediawiki/parts/7/77/T--INSA-UPS_France--pGAP-D-NY15.png" alt="">
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      <figcaption>
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      Figure 2: construction to characterized BBa_K431009.
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      </figcaption>
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    </figure>
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    <p>
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    In this part encoding sequence of D-NY15 was placed under the yeast constitutive promoter pGAP. This part was characterized by RT-qPCR.
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    </p>
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    <p>
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      <b>To learn more: </b> see </html><partinfo>BBa_K431009</partinfo><html> and our <a href="https://2017.igem.org/Team:INSA-UPS_France/Results">results</a>.
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    </p>
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<br><br>
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<br><br>
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  <h2></html><partinfo>BBa_K1800001</partinfo><html>: alpha factor secretion signal </h2>
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    <figure>
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      <img src="https://static.igem.org/mediawiki/parts/1/19/T--INSA-UPS_France--K1800001.png
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" alt="">
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      <figcaption>
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      Figure 3: construction to characterized BBa_K1800001.
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      </figcaption>
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    </figure>
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    <p>
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The α-factor sequence contains a kozak region and a signal sequence to secrete the produced peptides. The functionality of the signal factor was investigated by demonstrating that AMP are present in the supernatant using a toxicity assay.
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    </p>
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    <p>
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<b>To learn more: </b> see </html><partinfo>BBa_K180001</partinfo><html>  and our <a href="https://2017.igem.org/Team:INSA-UPS_France/Results">results</a>.
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<br><br>
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<br><br>
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<h2><i>Pichia pastoris</i> complete module with reporter gene : Odr-10 diacetyl receptor (</html><partinfo>BBa_K1072010</partinfo>), pFUS1 (<partinfo>BBa_K1072023</partinfo><html>)
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</h2>
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    <figure>
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      <img src="https://static.igem.org/mediawiki/parts/3/37/T--INSA-UPS_France--ODR-10-rfp.png" alt="">
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      <figcaption>
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      </figcaption>
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    </figure>
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    <p>
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This part includes Odr-10 receptor under the control of pGAP, a constitutive yeast promoter. When diacetyl binds to Odr-10 a cascade of activation of Ste proteins (endogenous to <i> P. pastoris </i>) will lead to the binding of Ste12 on pFUS1 promoter, and the expression of RFP should be activated.
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<br><br>
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This system allowed to produce RFP in response to diacetyl.
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<br><br>
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This construction was cloned in pPICZalpha yeast vector.
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    </p>
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    <p>
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      <b>To learn more:</b> see </html><partinfo>BBa_K1072010</partinfo>, <partinfo>BBa_K1072023</partinfo><html> and our <a href="https://2017.igem.org/Team:INSA-UPS_France/results">results</a>.
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Revision as of 17:26, 1 November 2017

Croc'n Cholera
iGEM UPS-INSA Toulouse 2017

Parts



*** All parts listed here are available upon request ***

Feel free to contact us, if you want to know more about our parts: igem.toulouse@gmail.com





Existing Parts we have contributed to characterized



<partinfo>BBa_J04450</partinfo>: RFP coding device

Figure 1: BBa_J04450 biobrick conjugated in Vibrio harveyi.

BBa_J04450 was tested in the Vibrio harveyi background. The biobrick was cloned in a broad host range plasmid (pBBR1MCS-4) and conjugated into Vibrio harveyi to demonstrate the production of RFP in this chassis.

To learn more: see <partinfo>BBa_J04450</partinfo> and our results.



<partinfo>BBa_K431009</partinfo>: glyceraldehyde 3-phosphate dehydrogenase promoter (pGAP)

Figure 2: construction to characterized BBa_K431009.

In this part encoding sequence of D-NY15 was placed under the yeast constitutive promoter pGAP. This part was characterized by RT-qPCR.

To learn more: see <partinfo>BBa_K431009</partinfo> and our results.





<partinfo>BBa_K1800001</partinfo>: alpha factor secretion signal

Figure 3: construction to characterized BBa_K1800001.

The α-factor sequence contains a kozak region and a signal sequence to secrete the produced peptides. The functionality of the signal factor was investigated by demonstrating that AMP are present in the supernatant using a toxicity assay.

To learn more: see <partinfo>BBa_K180001</partinfo> and our results.



Pichia pastoris complete module with reporter gene : Odr-10 diacetyl receptor (<partinfo>BBa_K1072010</partinfo>), pFUS1 (<partinfo>BBa_K1072023</partinfo>)

This part includes Odr-10 receptor under the control of pGAP, a constitutive yeast promoter. When diacetyl binds to Odr-10 a cascade of activation of Ste proteins (endogenous to P. pastoris ) will lead to the binding of Ste12 on pFUS1 promoter, and the expression of RFP should be activated.

This system allowed to produce RFP in response to diacetyl.

This construction was cloned in pPICZalpha yeast vector.

To learn more: see <partinfo>BBa_K1072010</partinfo>, <partinfo>BBa_K1072023</partinfo> and our results.