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+ | {{Heidelberg/templateus/Imagebox|https://static.igem.org/mediawiki/2017/4/40/T--Heidelberg--PlateAssay.png|Figure 3:|The riboswitch binding assay in a 96-well plate immediately after substrate addition. Rows show biological triplicates (n = 3) and riboswitch activators are compound (3) ethyl 2-(dimethyl(phenyl)silyl)propanoate and compound (1) dimethyl(phenyl)silane.|}} | ||
{{Heidelberg/partspanelleft|{{#tag:html|<a href="http://parts.igem.org/Part:BBa_K2398000">BBa_K2398000</a>}}|We present as best basic part the codon-optimized version of the cytochrome c protein that is able to convert silicon educts to organosilicon products.|https://static.igem.org/mediawiki/2017/1/1d/T--Heidelberg--CytochromeCRMA.png|}} | {{Heidelberg/partspanelleft|{{#tag:html|<a href="http://parts.igem.org/Part:BBa_K2398000">BBa_K2398000</a>}}|We present as best basic part the codon-optimized version of the cytochrome c protein that is able to convert silicon educts to organosilicon products.|https://static.igem.org/mediawiki/2017/1/1d/T--Heidelberg--CytochromeCRMA.png|}} | ||
Revision as of 17:27, 1 November 2017
{{#tag:html| {{Heidelberg/templateus/Contentsection|
![](https://static.igem.org/mediawiki/2017/4/40/T--Heidelberg--PlateAssay.png)
Figure 3:
The riboswitch binding assay in a 96-well plate immediately after substrate addition. Rows show biological triplicates (n = 3) and riboswitch activators are compound (3) ethyl 2-(dimethyl(phenyl)silyl)propanoate and compound (1) dimethyl(phenyl)silane.
BBa_K2398000
We present as best basic part the codon-optimized version of the cytochrome c protein that is able to convert silicon educts to organosilicon products.![Circularization Construct](https://static.igem.org/mediawiki/2017/1/1d/T--Heidelberg--CytochromeCRMA.png)
{{{5}}}
![](https://static.igem.org/mediawiki/parts/9/92/T--Heidelberg--GCAnilineCompound.png)
Figure 1:
Gas chromatogram for the reaction of educt (1) and (5) to the product (3). 11.7 minutes retention time, indicates product formation. Unconverted educts converge 6.9 and 7.2, 7.4 minutes
![](https://static.igem.org/mediawiki/parts/1/16/T--Heidelberg--MSAnilineCompound.png)
Figure 2:
Mass chromatogram shows the breakdown of the product (3) ethyl 2-((4-aminophenyl)dimethylsilyl)propanoate. The product itself corresponds to a mass of 251 dalton
![](https://static.igem.org/mediawiki/parts/e/ef/T--Heidelberg--GCNonAninlineCompound.png)
Figure 3:
Gas chromatogram for the reaction of educt (2) and (5) to the product (4). 9.2 minutes retention time indicates product formation.
![](https://static.igem.org/mediawiki/parts/2/2b/T--Heidelberg--MSNonAnilineCompound.png)
Figure 4:
Mass chromatogram shows the breakdown of the product (4) ethyl 2-(dimethyl(phenyl)silyl)propanoate. The product itself corresponds to a mass of 236 dalton