Difference between revisions of "Team:XMU-China/Demonstrate"

 
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<li><a href="https://2017.igem.org/Team:XMU-China/Demonstrate">Demonstrate</a></li>
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Demonstrate
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<p>Combining our project with the freeze-dried engineering bacteria and East-Wind, we found that our project can be used to detect heavy metals in water. In order to link up all the experimental contents and show you the testing process clearly, we drew a flow chart below.<br /><br />
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<span class="demonstrateimg"><img class="demonstrateimg1" src="https://static.igem.org/mediawiki/2017/1/13/T--XMU-China--demonstrateimg1.jpeg"></span><br /><br />
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        <p>The team's project description must be documented on their iGEM Team Wiki by the deadline. The project description should be 1-3 paragraphs and only needs to include an up-to-date explanation of the project. This description should be posted on your Team Wiki home page (example URL: https://2017.igem.org/Team:Example).This description will not be used to judge your project. It serves to provide some background on what your team has been working on so far and what you hope to accomplish. It also provides other teams the opportunity to see what you're working on so they can reach out for collaborations or troubleshooting if they are exploring a similar topic. </p>
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Here are several possible issues that maybe influence the detection in a real environment. Of course, these variates have been controlled in ideal conditions. You can click <a href="https://2017.igem.org/Team:XMU-China/Results">here</a> to check our results.<br />
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1. pH of water samples<br />
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2. Temperature of water samples<br />
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3. Colored contaminants and suspended in water samples<br />
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        <p>Once the wikis are frozen, users can no longer make any changes until they are unfrozen after the Jamboree. Please be sure to read through the the wiki requirements! No extensions will be provided (no exceptions!) and judges will begin their assessment on November 2, so make sure all changes are done by 11:59PM EDT.PLEASE NOTE: During week of wiki freeze, iGEM experiences higher than normal traffic which can lead to strain on our servers and sessions timing out for users. iGEM HQ recommends that users do not wait till the last days to work on and/or finish their wikis. Edits done during the final week should be very minor edits (spelling mistakes, updating images, etc.). </p>
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4. Establishment of standard curves<br />
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5. Biosecurity<br /><br />
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To address these issues, we propose following solutions.<br /><br />
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1. As we all know, the most suitable pH for engineering bacteria is 6.0-8.0. Through Chinese government’s water-test website, we found that the pH of most water check point is 7.0-7.9, which means our engineering bacteria can grow normally in most cases directly. We can also test the water samples’ pH by using pH test strips. Even if they cannot grow normally due to the pH of water samples, at least the fact has proved that you’d better not drink water here and these water samples are worth having them inspected professionally.<br /><br />
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        <p>All iGEM 2017 Team Wikis are required to have the iGEM Login Bar. The Login Bar includes essential links, tools, and information for all users: both wiki creators and viewers. The judges need these tools.
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2. Actually we designed a constant temperature circuit in East-Wind. According to the ambient you can choose to use it or not. You can click <a href="https://2017.igem.org/Team:XMU-China/East-Wind">here</a> to know more about our hardware East-Wind.<br /><br />
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The iGEM Login Bar is a 20 pixel high, full-width menu bar that exists on all 2017.igem.org pages. It contains: iGEM related pages, wiki tools (edit, history, watch, etc.), search tools, team information (not released yet), login, and the site menu. It serves as a quick navigation tool for all users and must be maintained on all of your team wiki pages as a result. </p>
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3. In the natural environment, the water samples are usually colorless transparent liquid. If the water samples are cloudy, probably because of mud. So just let the water sample sit and take the supernatant to test. If water samples contain colored contaminants, you’d better not drink water here and the water samples are worth having them inspected professionally.<br /><br />
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4. Select two microfluidic chips as the control group, add pure water into one chip and standard solution into another. You can compare the data of your water samples with that of the control group to reach your conclusion.<br /><br />
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The purpose of our project is definitely not to replace professional testing. If our project can help people make preliminary judgments of water samples and save their time and money, it will inspire us a lot.<br /><br />
 +
5. As we mentioned above, the engineering bacteria are in the state of freeze-dried powder. They have been placed in the microfluidic chips which are hermetic in the laboratory before you use them in  practice. The microfluidic chips are disposable, so that they will not be exposed to biological safety problems if you collect them appropriately after using them.</p>
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<p>Xiamen University，Fujian,China</p>
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<p>Xiamen University, Fujian, China<br />
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<p>No. 422, Siming South Road, Xiamen, Fujian, P. R. China 361005</p>
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No. 422, Siming South Road, Xiamen, Fujian, P. R. China 361005</p>
 
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Latest revision as of 17:39, 1 November 2017

2017.igem.org/Team:XMU-China/Demonstrate



Combining our project with the freeze-dried engineering bacteria and East-Wind, we found that our project can be used to detect heavy metals in water. In order to link up all the experimental contents and show you the testing process clearly, we drew a flow chart below.



Here are several possible issues that maybe influence the detection in a real environment. Of course, these variates have been controlled in ideal conditions. You can click here to check our results.
1. pH of water samples
2. Temperature of water samples
3. Colored contaminants and suspended in water samples
4. Establishment of standard curves
5. Biosecurity

To address these issues, we propose following solutions.

1. As we all know, the most suitable pH for engineering bacteria is 6.0-8.0. Through Chinese government’s water-test website, we found that the pH of most water check point is 7.0-7.9, which means our engineering bacteria can grow normally in most cases directly. We can also test the water samples’ pH by using pH test strips. Even if they cannot grow normally due to the pH of water samples, at least the fact has proved that you’d better not drink water here and these water samples are worth having them inspected professionally.

2. Actually we designed a constant temperature circuit in East-Wind. According to the ambient you can choose to use it or not. You can click here to know more about our hardware East-Wind.

3. In the natural environment, the water samples are usually colorless transparent liquid. If the water samples are cloudy, probably because of mud. So just let the water sample sit and take the supernatant to test. If water samples contain colored contaminants, you’d better not drink water here and the water samples are worth having them inspected professionally.

4. Select two microfluidic chips as the control group, add pure water into one chip and standard solution into another. You can compare the data of your water samples with that of the control group to reach your conclusion.

The purpose of our project is definitely not to replace professional testing. If our project can help people make preliminary judgments of water samples and save their time and money, it will inspire us a lot.

5. As we mentioned above, the engineering bacteria are in the state of freeze-dried powder. They have been placed in the microfluidic chips which are hermetic in the laboratory before you use them in practice. The microfluidic chips are disposable, so that they will not be exposed to biological safety problems if you collect them appropriately after using them.