Difference between revisions of "Biosafety.html"

(Created page with "<!DOCTYPE html> <html> <head> <title>DEI AGRA Wiki Page</title> <meta name="description" content="website description" /> <meta name="keywords" content="website keywor...")
 
 
(4 intermediate revisions by the same user not shown)
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<!DOCTYPE html>
+
<html xmlns:v="urn:schemas-microsoft-com:vml"
−
<html>
+
xmlns:o="urn:schemas-microsoft-com:office:office"
 +
xmlns:w="urn:schemas-microsoft-com:office:word"
 +
xmlns:m="http://schemas.microsoft.com/office/2004/12/omml"
 +
xmlns="http://www.w3.org/TR/REC-html40">
  
 
<head>
 
<head>
−
  <title>DEI AGRA Wiki Page</title>
+
<meta http-equiv=Content-Type content="text/html; charset=windows-1252">
−
  <meta name="description" content="website description" />
+
<meta name=ProgId content=Word.Document>
−
   <meta name="keywords" content="website keywords, website keywords" />
+
<meta name=Generator content="Microsoft Word 15">
−
   <meta http-equiv="content-type" content="text/html; charset=windows-1252" />
+
<meta name=Originator content="Microsoft Word 15">
−
  <link rel="stylesheet" type="text/css" href="css/style.css" />
+
<link rel=File-List href="Biosafety_files/filelist.xml">
−
   <!-- modernizr enables HTML5 elements and feature detects -->
+
<link rel=Edit-Time-Data href="Biosafety_files/editdata.mso">
−
   <script type="text/javascript" src="js/modernizr-1.5.min.js"></script>
+
<!--[if !mso]>
 +
<style>
 +
v\:* {behavior:url(#default#VML);}
 +
o\:* {behavior:url(#default#VML);}
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w\:* {behavior:url(#default#VML);}
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.shape {behavior:url(#default#VML);}
 +
</style>
 +
<![endif]--><!--[if gte mso 9]><xml>
 +
<o:DocumentProperties>
 +
   <o:Author>Shubhodaya Sharma</o:Author>
 +
  <o:Template>Normal</o:Template>
 +
  <o:LastAuthor>Shubhodaya Sharma</o:LastAuthor>
 +
  <o:Revision>2</o:Revision>
 +
  <o:TotalTime>61</o:TotalTime>
 +
  <o:LastPrinted>2017-08-18T04:37:00Z</o:LastPrinted>
 +
  <o:Created>2017-11-01T14:21:00Z</o:Created>
 +
  <o:LastSaved>2017-11-01T14:21:00Z</o:LastSaved>
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  <o:Pages>1</o:Pages>
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  <o:Words>801</o:Words>
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  <o:Characters>4570</o:Characters>
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  <o:CharactersWithSpaces>5361</o:CharactersWithSpaces>
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  <o:Version>15.00</o:Version>
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</o:DocumentProperties>
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<o:OfficeDocumentSettings>
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  <o:RelyOnVML/>
 +
  <o:AllowPNG/>
 +
</o:OfficeDocumentSettings>
 +
</xml><![endif]-->
 +
<link rel=dataStoreItem href="Biosafety_files/item0001.xml"
 +
target="Biosafety_files/props002.xml">
 +
<link rel=themeData href="Biosafety_files/themedata.thmx">
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<link rel=colorSchemeMapping href="Biosafety_files/colorschememapping.xml">
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<w:WordDocument>
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   <w:SpellingState>Clean</w:SpellingState>
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  <w:GrammarState>Clean</w:GrammarState>
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  <w:TrackMoves>false</w:TrackMoves>
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  <w:TrackFormatting/>
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  <w:DrawingGridVerticalSpacing>6 pt</w:DrawingGridVerticalSpacing>
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  <w:UseMarginsForDrawingGridOrigin/>
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  <w:DoNotShadeFormData/>
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  <w:Compatibility>
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  <w:SpaceForUL/>
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  <w:BalanceSingleByteDoubleByteWidth/>
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  <w:BreakWrappedTables/>
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  <w:DontGrowAutofit/>
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  <w:SplitPgBreakAndParaMark/>
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  <w:EnableOpenTypeKerning/>
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  <w:DontFlipMirrorIndents/>
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  <w:OverrideTableStyleHps/>
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  <w:UseFELayout/>
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  </w:Compatibility>
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  <m:mathPr>
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  <m:mathFont m:val="Cambria Math"/>
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  <m:brkBin m:val="before"/>
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  <m:brkBinSub m:val="&#45;-"/>
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 +
  <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
 +
  Name="Table List 3"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
 +
  Name="Table List 4"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
 +
  Name="Table List 5"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
 +
  Name="Table List 6"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
 +
  Name="Table List 7"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
 +
  Name="Table List 8"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
 +
  Name="Table 3D effects 1"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
 +
  Name="Table 3D effects 2"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
 +
  Name="Table 3D effects 3"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
 +
  Name="Table Contemporary"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
 +
  Name="Table Elegant"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
 +
  Name="Table Professional"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
 +
  Name="Table Subtle 1"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
 +
  Name="Table Subtle 2"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
 +
  Name="Table Web 1"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
 +
  Name="Table Web 2"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
 +
  Name="Table Web 3"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
 +
  Name="Balloon Text"/>
 +
  <w:LsdException Locked="false" Priority="39" Name="Table Grid"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" UnhideWhenUsed="true"
 +
  Name="Table Theme"/>
 +
  <w:LsdException Locked="false" SemiHidden="true" Name="Placeholder Text"/>
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  <w:LsdException Locked="false" Priority="1" QFormat="true" Name="No Spacing"/>
 +
  <w:LsdException Locked="false" Priority="60" Name="Light Shading"/>
 +
  <w:LsdException Locked="false" Priority="61" Name="Light List"/>
 +
  <w:LsdException Locked="false" Priority="62" Name="Light Grid"/>
 +
  <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1"/>
 +
  <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2"/>
 +
  <w:LsdException Locked="false" Priority="65" Name="Medium List 1"/>
 +
  <w:LsdException Locked="false" Priority="66" Name="Medium List 2"/>
 +
  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1"/>
 +
  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2"/>
 +
  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3"/>
 +
  <w:LsdException Locked="false" Priority="70" Name="Dark List"/>
 +
  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading"/>
 +
  <w:LsdException Locked="false" Priority="72" Name="Colorful List"/>
 +
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid"/>
 +
  <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="61" Name="Light List Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 1"/>
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  <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 1"/>
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  <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 1"/>
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  <w:LsdException Locked="false" SemiHidden="true" Name="Revision"/>
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  <w:LsdException Locked="false" Priority="34" QFormat="true"
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  Name="List Paragraph"/>
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  <w:LsdException Locked="false" Priority="29" QFormat="true" Name="Quote"/>
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  <w:LsdException Locked="false" Priority="30" QFormat="true"
 +
  Name="Intense Quote"/>
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  <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 1"/>
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  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 1"/>
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  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 1"/>
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  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 1"/>
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  <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 1"/>
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  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 1"/>
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  <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 1"/>
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  <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="61" Name="Light List Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 2"/>
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  <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 2"/>
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  <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 2"/>
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  <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 2"/>
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  <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="61" Name="Light List Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="61" Name="Light List Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 4"/>
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  <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="61" Name="Light List Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 5"/>
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  <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 5"/>
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  <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 5"/>
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  <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 5"/>
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  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 5"/>
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  <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 5"/>
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  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 5"/>
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  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="60" Name="Light Shading Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="61" Name="Light List Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="62" Name="Light Grid Accent 6"/>
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  <w:LsdException Locked="false" Priority="63" Name="Medium Shading 1 Accent 6"/>
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  <w:LsdException Locked="false" Priority="64" Name="Medium Shading 2 Accent 6"/>
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  <w:LsdException Locked="false" Priority="65" Name="Medium List 1 Accent 6"/>
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  <w:LsdException Locked="false" Priority="66" Name="Medium List 2 Accent 6"/>
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  <w:LsdException Locked="false" Priority="67" Name="Medium Grid 1 Accent 6"/>
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  <w:LsdException Locked="false" Priority="68" Name="Medium Grid 2 Accent 6"/>
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  <w:LsdException Locked="false" Priority="69" Name="Medium Grid 3 Accent 6"/>
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  <w:LsdException Locked="false" Priority="70" Name="Dark List Accent 6"/>
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  <w:LsdException Locked="false" Priority="71" Name="Colorful Shading Accent 6"/>
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  <w:LsdException Locked="false" Priority="72" Name="Colorful List Accent 6"/>
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  <w:LsdException Locked="false" Priority="73" Name="Colorful Grid Accent 6"/>
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  <w:LsdException Locked="false" Priority="19" QFormat="true"
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  Name="Subtle Emphasis"/>
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  <w:LsdException Locked="false" Priority="21" QFormat="true"
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  Name="Intense Emphasis"/>
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  <w:LsdException Locked="false" Priority="31" QFormat="true"
 +
  Name="Subtle Reference"/>
 +
  <w:LsdException Locked="false" Priority="32" QFormat="true"
 +
  Name="Intense Reference"/>
 +
  <w:LsdException Locked="false" Priority="33" QFormat="true" Name="Book Title"/>
 +
  <w:LsdException Locked="false" Priority="37" SemiHidden="true"
 +
  UnhideWhenUsed="true" Name="Bibliography"/>
 +
  <w:LsdException Locked="false" Priority="39" SemiHidden="true"
 +
  UnhideWhenUsed="true" QFormat="true" Name="TOC Heading"/>
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  <w:LsdException Locked="false" Priority="41" Name="Plain Table 1"/>
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  <w:LsdException Locked="false" Priority="42" Name="Plain Table 2"/>
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  <w:LsdException Locked="false" Priority="43" Name="Plain Table 3"/>
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  <w:LsdException Locked="false" Priority="44" Name="Plain Table 4"/>
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  <w:LsdException Locked="false" Priority="45" Name="Plain Table 5"/>
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  <w:LsdException Locked="false" Priority="40" Name="Grid Table Light"/>
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  <w:LsdException Locked="false" Priority="46" Name="Grid Table 1 Light"/>
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  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2"/>
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  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3"/>
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  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4"/>
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  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark"/>
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  <w:LsdException Locked="false" Priority="51" Name="Grid Table 6 Colorful"/>
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  <w:LsdException Locked="false" Priority="52" Name="Grid Table 7 Colorful"/>
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  <w:LsdException Locked="false" Priority="46"
 +
  Name="Grid Table 1 Light Accent 1"/>
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  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 1"/>
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  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 1"/>
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  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 1"/>
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  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 1"/>
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  <w:LsdException Locked="false" Priority="51"
 +
  Name="Grid Table 6 Colorful Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="Grid Table 7 Colorful Accent 1"/>
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  <w:LsdException Locked="false" Priority="46"
 +
  Name="Grid Table 1 Light Accent 2"/>
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  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 2"/>
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  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 2"/>
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  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 2"/>
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  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 2"/>
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  <w:LsdException Locked="false" Priority="51"
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  Name="Grid Table 6 Colorful Accent 2"/>
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  <w:LsdException Locked="false" Priority="52"
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  Name="Grid Table 7 Colorful Accent 2"/>
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  <w:LsdException Locked="false" Priority="46"
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  Name="Grid Table 1 Light Accent 3"/>
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  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 3"/>
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  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 3"/>
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  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 3"/>
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  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 3"/>
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  <w:LsdException Locked="false" Priority="51"
 +
  Name="Grid Table 6 Colorful Accent 3"/>
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  <w:LsdException Locked="false" Priority="52"
 +
  Name="Grid Table 7 Colorful Accent 3"/>
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  <w:LsdException Locked="false" Priority="46"
 +
  Name="Grid Table 1 Light Accent 4"/>
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  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 4"/>
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  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 4"/>
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  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 4"/>
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  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 4"/>
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  <w:LsdException Locked="false" Priority="51"
 +
  Name="Grid Table 6 Colorful Accent 4"/>
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  <w:LsdException Locked="false" Priority="52"
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  Name="Grid Table 7 Colorful Accent 4"/>
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  <w:LsdException Locked="false" Priority="46"
 +
  Name="Grid Table 1 Light Accent 5"/>
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  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 5"/>
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  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 5"/>
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  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 5"/>
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  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 5"/>
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  <w:LsdException Locked="false" Priority="51"
 +
  Name="Grid Table 6 Colorful Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="Grid Table 7 Colorful Accent 5"/>
 +
  <w:LsdException Locked="false" Priority="46"
 +
  Name="Grid Table 1 Light Accent 6"/>
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  <w:LsdException Locked="false" Priority="47" Name="Grid Table 2 Accent 6"/>
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  <w:LsdException Locked="false" Priority="48" Name="Grid Table 3 Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="49" Name="Grid Table 4 Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="50" Name="Grid Table 5 Dark Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="51"
 +
  Name="Grid Table 6 Colorful Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="Grid Table 7 Colorful Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="46" Name="List Table 1 Light"/>
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  <w:LsdException Locked="false" Priority="47" Name="List Table 2"/>
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  <w:LsdException Locked="false" Priority="48" Name="List Table 3"/>
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  <w:LsdException Locked="false" Priority="49" Name="List Table 4"/>
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  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark"/>
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  <w:LsdException Locked="false" Priority="51" Name="List Table 6 Colorful"/>
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  <w:LsdException Locked="false" Priority="52" Name="List Table 7 Colorful"/>
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  <w:LsdException Locked="false" Priority="46"
 +
  Name="List Table 1 Light Accent 1"/>
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  <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 1"/>
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  <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 1"/>
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  <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 1"/>
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  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="51"
 +
  Name="List Table 6 Colorful Accent 1"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="List Table 7 Colorful Accent 1"/>
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  <w:LsdException Locked="false" Priority="46"
 +
  Name="List Table 1 Light Accent 2"/>
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  <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 2"/>
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  <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 2"/>
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  <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 2"/>
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  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="51"
 +
  Name="List Table 6 Colorful Accent 2"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="List Table 7 Colorful Accent 2"/>
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  <w:LsdException Locked="false" Priority="46"
 +
  Name="List Table 1 Light Accent 3"/>
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  <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 3"/>
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  <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 3"/>
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  <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 3"/>
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  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 3"/>
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  <w:LsdException Locked="false" Priority="51"
 +
  Name="List Table 6 Colorful Accent 3"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="List Table 7 Colorful Accent 3"/>
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  <w:LsdException Locked="false" Priority="46"
 +
  Name="List Table 1 Light Accent 4"/>
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  <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 4"/>
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  <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 4"/>
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  <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 4"/>
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  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="51"
 +
  Name="List Table 6 Colorful Accent 4"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="List Table 7 Colorful Accent 4"/>
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  <w:LsdException Locked="false" Priority="46"
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  Name="List Table 1 Light Accent 5"/>
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  <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 5"/>
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  <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 5"/>
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  <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 5"/>
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  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 5"/>
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  <w:LsdException Locked="false" Priority="51"
 +
  Name="List Table 6 Colorful Accent 5"/>
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  <w:LsdException Locked="false" Priority="52"
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  Name="List Table 7 Colorful Accent 5"/>
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  <w:LsdException Locked="false" Priority="46"
 +
  Name="List Table 1 Light Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="47" Name="List Table 2 Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="48" Name="List Table 3 Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="49" Name="List Table 4 Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="50" Name="List Table 5 Dark Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="51"
 +
  Name="List Table 6 Colorful Accent 6"/>
 +
  <w:LsdException Locked="false" Priority="52"
 +
  Name="List Table 7 Colorful Accent 6"/>
 +
</w:LatentStyles>
 +
</xml><![endif]-->
 +
<style>
 +
<!--
 +
/* Font Definitions */
 +
@font-face
 +
{font-family:Wingdings;
 +
panose-1:5 0 0 0 0 0 0 0 0 0;
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mso-font-charset:2;
 +
mso-generic-font-family:auto;
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mso-font-pitch:variable;
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mso-font-signature:0 268435456 0 0 -2147483648 0;}
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@font-face
 +
{font-family:Mangal;
 +
panose-1:2 4 5 3 5 2 3 3 2 2;
 +
mso-font-charset:0;
 +
mso-generic-font-family:roman;
 +
mso-font-pitch:variable;
 +
mso-font-signature:32771 0 0 0 1 0;}
 +
@font-face
 +
{font-family:"Cambria Math";
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panose-1:2 4 5 3 5 4 6 3 2 4;
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mso-font-charset:1;
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mso-generic-font-family:roman;
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mso-font-format:other;
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mso-font-pitch:variable;
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mso-font-signature:0 0 0 0 0 0;}
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@font-face
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{font-family:Tahoma;
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panose-1:2 11 6 4 3 5 4 4 2 4;
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mso-font-charset:0;
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mso-generic-font-family:swiss;
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mso-font-pitch:variable;
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mso-font-signature:-520081665 -1073717157 41 0 66047 0;}
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/* Style Definitions */
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p.MsoNormal, li.MsoNormal, div.MsoNormal
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{mso-style-unhide:no;
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mso-style-qformat:yes;
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mso-style-parent:"";
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margin-top:0in;
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margin-right:0in;
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margin-bottom:8.0pt;
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margin-left:0in;
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line-height:107%;
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mso-pagination:widow-orphan;
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font-size:11.0pt;
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mso-bidi-font-size:10.0pt;
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font-family:"Calibri",sans-serif;
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mso-ascii-font-family:Calibri;
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mso-ascii-theme-font:minor-latin;
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mso-fareast-font-family:"Times New Roman";
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mso-fareast-theme-font:minor-fareast;
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mso-hansi-font-family:Calibri;
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mso-hansi-theme-font:minor-latin;
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mso-bidi-font-family:Mangal;}
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p.MsoAcetate, li.MsoAcetate, div.MsoAcetate
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{mso-style-noshow:yes;
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mso-style-priority:99;
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mso-style-link:"Balloon Text Char";
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margin:0in;
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margin-bottom:.0001pt;
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mso-pagination:widow-orphan;
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font-size:8.0pt;
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mso-bidi-font-size:7.0pt;
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font-family:"Tahoma",sans-serif;
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mso-fareast-font-family:"Times New Roman";
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mso-fareast-theme-font:minor-fareast;
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mso-bidi-font-family:Mangal;}
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p.MsoListParagraph, li.MsoListParagraph, div.MsoListParagraph
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{mso-style-priority:34;
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mso-style-unhide:no;
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mso-style-qformat:yes;
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margin-top:0in;
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margin-right:0in;
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margin-bottom:8.0pt;
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margin-left:.5in;
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mso-add-space:auto;
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line-height:107%;
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mso-pagination:widow-orphan;
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font-size:11.0pt;
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mso-bidi-font-size:10.0pt;
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font-family:"Calibri",sans-serif;
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mso-ascii-font-family:Calibri;
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mso-ascii-theme-font:minor-latin;
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mso-fareast-font-family:"Times New Roman";
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mso-fareast-theme-font:minor-fareast;
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mso-hansi-font-family:Calibri;
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mso-hansi-theme-font:minor-latin;
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mso-bidi-font-family:Mangal;}
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p.MsoListParagraphCxSpFirst, li.MsoListParagraphCxSpFirst, div.MsoListParagraphCxSpFirst
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{mso-style-priority:34;
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mso-style-unhide:no;
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mso-style-qformat:yes;
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mso-style-type:export-only;
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margin-top:0in;
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margin-right:0in;
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margin-bottom:0in;
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margin-left:.5in;
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margin-bottom:.0001pt;
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mso-add-space:auto;
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line-height:107%;
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mso-pagination:widow-orphan;
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font-size:11.0pt;
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mso-bidi-font-size:10.0pt;
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font-family:"Calibri",sans-serif;
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mso-ascii-font-family:Calibri;
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mso-ascii-theme-font:minor-latin;
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mso-fareast-font-family:"Times New Roman";
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mso-fareast-theme-font:minor-fareast;
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mso-hansi-font-family:Calibri;
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mso-hansi-theme-font:minor-latin;
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mso-bidi-font-family:Mangal;}
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p.MsoListParagraphCxSpMiddle, li.MsoListParagraphCxSpMiddle, div.MsoListParagraphCxSpMiddle
 +
{mso-style-priority:34;
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mso-style-unhide:no;
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mso-style-qformat:yes;
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mso-style-type:export-only;
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margin-top:0in;
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margin-right:0in;
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margin-bottom:0in;
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margin-left:.5in;
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margin-bottom:.0001pt;
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mso-add-space:auto;
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line-height:107%;
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mso-pagination:widow-orphan;
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font-size:11.0pt;
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mso-bidi-font-size:10.0pt;
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font-family:"Calibri",sans-serif;
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mso-ascii-font-family:Calibri;
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mso-ascii-theme-font:minor-latin;
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mso-fareast-font-family:"Times New Roman";
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mso-fareast-theme-font:minor-fareast;
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mso-hansi-font-family:Calibri;
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mso-hansi-theme-font:minor-latin;
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mso-bidi-font-family:Mangal;}
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p.MsoListParagraphCxSpLast, li.MsoListParagraphCxSpLast, div.MsoListParagraphCxSpLast
 +
{mso-style-priority:34;
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mso-style-unhide:no;
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mso-style-qformat:yes;
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mso-style-type:export-only;
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margin-top:0in;
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margin-right:0in;
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margin-bottom:8.0pt;
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margin-left:.5in;
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mso-add-space:auto;
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line-height:107%;
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mso-pagination:widow-orphan;
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font-size:11.0pt;
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mso-bidi-font-size:10.0pt;
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font-family:"Calibri",sans-serif;
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mso-ascii-font-family:Calibri;
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mso-ascii-theme-font:minor-latin;
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mso-fareast-font-family:"Times New Roman";
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mso-fareast-theme-font:minor-fareast;
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mso-hansi-font-family:Calibri;
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mso-hansi-theme-font:minor-latin;
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mso-bidi-font-family:Mangal;}
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span.BalloonTextChar
 +
{mso-style-name:"Balloon Text Char";
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mso-style-noshow:yes;
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mso-style-priority:99;
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mso-style-unhide:no;
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mso-style-locked:yes;
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mso-style-link:"Balloon Text";
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mso-ansi-font-size:8.0pt;
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mso-bidi-font-size:7.0pt;
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font-family:"Tahoma",sans-serif;
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mso-ascii-font-family:Tahoma;
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mso-hansi-font-family:Tahoma;
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mso-bidi-font-family:Mangal;}
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span.SpellE
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{mso-style-name:"";
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mso-spl-e:yes;}
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span.GramE
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{mso-style-name:"";
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mso-gram-e:yes;}
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.MsoChpDefault
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{mso-style-type:export-only;
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mso-default-props:yes;
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mso-bidi-font-size:11.0pt;
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font-family:"Calibri",sans-serif;
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mso-ascii-font-family:Calibri;
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mso-ascii-theme-font:minor-latin;
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mso-fareast-font-family:"Times New Roman";
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mso-fareast-theme-font:minor-fareast;
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mso-hansi-font-family:Calibri;
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mso-hansi-theme-font:minor-latin;}
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.MsoPapDefault
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{mso-style-type:export-only;
 +
margin-bottom:8.0pt;
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line-height:107%;}
 +
/* Page Definitions */
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@page
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{mso-page-border-surround-header:no;
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mso-page-border-surround-footer:no;}
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@page WordSection1
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{size:8.5in 11.0in;
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margin:1.0in 1.0in 1.0in 1.0in;
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mso-header-margin:.5in;
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mso-footer-margin:.5in;
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mso-paper-source:0;}
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div.WordSection1
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{page:WordSection1;}
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/* List Definitions */
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@list l0
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{mso-list-id:-2;
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mso-list-type:simple;
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mso-list-template-ids:1634529418;}
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@list l0:level1
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{mso-level-start-at:0;
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mso-level-number-format:bullet;
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mso-level-text:*;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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margin-left:0in;
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text-indent:0in;}
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@list l1
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{mso-list-id:227419778;
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mso-list-type:hybrid;
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mso-list-template-ids:456160410 67698699 67698691 67698693 67698689 67698691 67698693 67698689 67698691 67698693;}
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@list l1:level1
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{mso-level-number-format:bullet;
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mso-level-text:\F0D8;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;
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font-family:Wingdings;}
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@list l1:level2
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{mso-level-number-format:bullet;
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mso-level-text:o;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;
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font-family:"Courier New";}
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@list l1:level3
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{mso-level-number-format:bullet;
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mso-level-text:\F0A7;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;
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font-family:Wingdings;}
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@list l1:level4
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{mso-level-number-format:bullet;
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mso-level-text:\F0B7;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;
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font-family:Symbol;}
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@list l1:level5
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{mso-level-number-format:bullet;
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mso-level-text:o;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;
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font-family:"Courier New";}
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@list l1:level6
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{mso-level-number-format:bullet;
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mso-level-text:\F0A7;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;
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font-family:Wingdings;}
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@list l1:level7
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{mso-level-number-format:bullet;
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mso-level-text:\F0B7;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;
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font-family:Symbol;}
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@list l1:level8
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{mso-level-number-format:bullet;
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mso-level-text:o;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;
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font-family:"Courier New";}
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@list l1:level9
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{mso-level-number-format:bullet;
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mso-level-text:\F0A7;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;
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font-family:Wingdings;}
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@list l2
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{mso-list-id:1314873939;
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mso-list-type:hybrid;
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mso-list-template-ids:-2031861262 1634529418 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
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@list l2:level1
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{mso-level-start-at:0;
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mso-level-number-format:bullet;
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mso-level-text:\F0B7;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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mso-level-legacy:yes;
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mso-level-legacy-indent:0in;
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mso-level-legacy-space:.25in;
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text-indent:-.25in;
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font-family:Symbol;}
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@list l2:level2
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{mso-level-number-format:alpha-lower;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;}
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@list l2:level3
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{mso-level-number-format:roman-lower;
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mso-level-tab-stop:none;
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mso-level-number-position:right;
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text-indent:-9.0pt;}
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@list l2:level4
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{mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;}
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@list l2:level5
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{mso-level-number-format:alpha-lower;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;}
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@list l2:level6
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{mso-level-number-format:roman-lower;
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mso-level-tab-stop:none;
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mso-level-number-position:right;
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text-indent:-9.0pt;}
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@list l2:level7
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{mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;}
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@list l2:level8
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{mso-level-number-format:alpha-lower;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;}
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@list l2:level9
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{mso-level-number-format:roman-lower;
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mso-level-tab-stop:none;
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mso-level-number-position:right;
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text-indent:-9.0pt;}
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@list l3
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{mso-list-id:1910312008;
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mso-list-type:hybrid;
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mso-list-template-ids:-195768726 1197656136 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
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@list l3:level1
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{mso-level-number-format:roman-lower;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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margin-left:.75in;
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text-indent:-.5in;}
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@list l3:level2
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{mso-level-number-format:alpha-lower;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;}
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@list l3:level3
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{mso-level-number-format:roman-lower;
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mso-level-tab-stop:none;
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mso-level-number-position:right;
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text-indent:-9.0pt;}
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@list l3:level4
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{mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;}
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@list l3:level5
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{mso-level-number-format:alpha-lower;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;}
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@list l3:level6
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{mso-level-number-format:roman-lower;
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mso-level-tab-stop:none;
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mso-level-number-position:right;
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text-indent:-9.0pt;}
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@list l3:level7
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{mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;}
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@list l3:level8
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{mso-level-number-format:alpha-lower;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;}
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@list l3:level9
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{mso-level-number-format:roman-lower;
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mso-level-tab-stop:none;
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mso-level-number-position:right;
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text-indent:-9.0pt;}
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@list l4
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{mso-list-id:1926644913;
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mso-list-type:hybrid;
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mso-list-template-ids:129687552 67698703 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}
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@list l4:level1
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{mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;}
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@list l4:level2
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{mso-level-number-format:alpha-lower;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;}
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@list l4:level3
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{mso-level-number-format:roman-lower;
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mso-level-tab-stop:none;
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mso-level-number-position:right;
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text-indent:-9.0pt;}
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@list l4:level4
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{mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;}
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@list l4:level5
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{mso-level-number-format:alpha-lower;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;}
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@list l4:level6
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{mso-level-number-format:roman-lower;
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mso-level-tab-stop:none;
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mso-level-number-position:right;
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text-indent:-9.0pt;}
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@list l4:level7
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{mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;}
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@list l4:level8
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{mso-level-number-format:alpha-lower;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;}
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@list l4:level9
 +
{mso-level-number-format:roman-lower;
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mso-level-tab-stop:none;
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mso-level-number-position:right;
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text-indent:-9.0pt;}
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@list l5
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{mso-list-id:1981841374;
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mso-list-type:hybrid;
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mso-list-template-ids:2124821242 67698689 67698691 67698693 67698689 67698691 67698693 67698689 67698691 67698693;}
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@list l5:level1
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{mso-level-number-format:bullet;
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mso-level-text:\F0B7;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;
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font-family:Symbol;}
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@list l5:level2
 +
{mso-level-number-format:bullet;
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mso-level-text:o;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;
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font-family:"Courier New";}
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@list l5:level3
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{mso-level-number-format:bullet;
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mso-level-text:\F0A7;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;
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font-family:Wingdings;}
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@list l5:level4
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{mso-level-number-format:bullet;
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mso-level-text:\F0B7;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;
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font-family:Symbol;}
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@list l5:level5
 +
{mso-level-number-format:bullet;
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mso-level-text:o;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;
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font-family:"Courier New";}
 +
@list l5:level6
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{mso-level-number-format:bullet;
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mso-level-text:\F0A7;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;
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font-family:Wingdings;}
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@list l5:level7
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{mso-level-number-format:bullet;
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mso-level-text:\F0B7;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;
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font-family:Symbol;}
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@list l5:level8
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{mso-level-number-format:bullet;
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mso-level-text:o;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;
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font-family:"Courier New";}
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@list l5:level9
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{mso-level-number-format:bullet;
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mso-level-text:\F0A7;
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mso-level-tab-stop:none;
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mso-level-number-position:left;
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text-indent:-.25in;
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font-family:Wingdings;}
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@list l6
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{mso-list-id:2121952162;
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mso-list-type:hybrid;
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mso-list-template-ids:-109127522 67698689 67698691 67698693 67698689 67698691 67698693 67698689 67698691 67698693;}
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@list l6:level1
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{mso-level-number-format:bullet;
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mso-level-text:\F0B7;
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</head>
 
</head>
  
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<body>
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<body lang=EN-US style='tab-interval:.5in;text-justify-trim:punctuation'><br/><br/>
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inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
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none;text-autospace:none'><b><span style='font-size:12.0pt;line-height:150%;
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font-family:"Times New Roman",serif'><span style='mso-spacerun:yes'>  </span>&quot;Small
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Mistakes in Biosafety could have terrible global consequences&quot;<o:p></o:p></span></b></p>
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<div id="site_content">
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          <li class="show"><img width="900" height="250" src="images/home_1.jpg" /></li>
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none;text-autospace:none'><b><i><span style='font-size:12.0pt;line-height:150%;
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style='mso-spacerun:yes'>                                   </span><span
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style='mso-spacerun:yes'>                          </span><span class=GramE>by</span>-
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  </div>
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<u>Thomas <span class=SpellE>Inglesky</span></u><o:p></o:p></span></i></b></p>
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  <div id="content">
+
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          <div class="content_item">
+
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  <div><h2 id='htd'> <b>"Small Mistakes in Biosafety could have terrible global consequences"</b></h2></div>
+
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  <div><h2 id='htd'> <b>By-Thomas Ingle sky</b></h2></div>
+
−
          <div><h1><strong>(Centre of biosecurity at UNIVERSITY OF PITTSBURGH)</strong></h1></div>
+
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+
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</div>
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<div class="content_imagetext1">
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+
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  <div>
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+
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<p>Biosafety refers to individual safeguards designed to protect laboratory personnel, as well as the surrounding environment and community. The biosafety protocols are divided into “BIOLOGICAL SAFETY LEVELS (BSL), which are series of protection relegated to autoclave related activities that take place in particular labs.</p>
+
−
    <p>These levels are generally standardized across the world, however their implementation varies from country to country, labs to labs, organization to organizations.</p>
+
−
    <p>These biosafety levels are ranked from 1 to 4, on the basis of agents or organisms that are being researched or worked on, in any given laboratory settings and are determined by the following-Risks related to containment, Severity of infection, Transmissibility, Nature of the work conducted, Origin of the microbe, Agent in question, Route of exposure</p>
+
−
            <h3><u><strong>BIOSAFETY IN INDIA</strong></u></h3>
+
−
            <p>IN India, a three tier mechanism comprising Institutional Biosafety Committees (IBSC) at the Institute/ company; the Review Committee on Genetic Manipulation (RCGM) in the Department of Biotechnology; and the Genetic Engineering Approval Committee (GEAC) in the Ministry of Environment & Forests (MoE&F), do analysis for granting approval for research and development activities on recombinant DNA products, environmental release of genetically engineered (GE)crops and monitoring and evaluation of research activities involving recombinant DNA technology has been established.</p>
+
−
    <p>In India, GMOs and products thereof are regulated as per the “Rules for the manufacture, use/import/export and storage of hazardous microorganisms/ genetically engineered organisms or cells, 1989” (commonly referred as Rules, 1989) notified by the Ministry of Environment and Forests (MoEF), Government of India under the Environment (Protection) Act (1986). These rules are implemented by MoEF, the Department of Biotechnology (DBT), Ministry of Science and Technology and the State Governments through the six competent authorities notified under the Rules which are as follows:</p>  
+
−
    <ul>
+
−
<li>Recombinant DNA Advisory Committee (RDAC)</li>
+
−
            <li>Institutional Biosafety Committee (IBSC)</li>
+
−
              <li> Review Committee on Genetic Manipulation (RCGM)</li>
+
−
                <li>Genetic Engineering Appraisal Committee (GEAC)</li>
+
−
                <li>State Biotechnology Coordination Committee (SBCC)</li>
+
−
                <li>District Level Committee (DLC).</li>
+
−
            </ul>
+
−
          <p>While the RDAC has advisory in function, IBSC, RCGM, and GEAC</p>
+
−
  </div>
+
−
+
−
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+
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+
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  <div class="content_item"><!-------start TEAM INSTRUCTORS-->
+
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          <div><h1><strong>FOLLOWING STEPS ARE INVOLVED IN THE ANALYSIS OF A GENETICALLY MODIFIED CELL -</strong></h1></div>
+
−
 
+
−
 
+
−
  <div class="content_imagetext1">
+
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    <div class="content_image1">
+
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      <img width="199" height="284"src="images/risk.png" alt="Risk"/>
+
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    </div>
+
−
 
+
−
  <div>
+
−
    <h3 id='htd'> <b>"BIOSAFETY AT DAYALBAGH EDUCATIONAL INSTITUTE"</b></h3>
+
−
<p>We at Dayalbagh Educational Institute understand the importance of Biosafety and strongly believe in its enforcement, therefore our lab works and experiments are carried out under strict biosafety precautions.</p>
+
−
            <p>Before commencing our wet lab experiments we had received a training sessions of two days on safety and precautions to be maintained while handling the Instruments, glassware’s and reagents in a laboratory. </p>
+
−
<p>For the iGEM project we are working with the E.coli strain (DH5 a and BL-21 ), which falls under BSL-1(Biosafety level 1). Therefore all BSL-1 are implemented and followed during our laboratory workings</p>
+
−
    <p><b>STERILIZATION:</b> Clean, Dirt free environment is essential for getting good results. We used sanitizers and 70% ethanol for cleaning the platform. </p>
+
−
            <p><b>TOXICITY RISK:</b> Ethidium Bromide is the most commonly used staining dye for DNA, is highly toxic as mutagens. It may potentially cause carcinogenic effects. So for our team project we had decided to use a less toxic dye “GEL RED” purchased from GCC biotech. </p>
+
−
            <p><b>HANDLING OF EQUIPMENT: </b> We used all necessary equipment’s such as Autoclave, U-V trans-illuminator, Biosafety Cabinet under the guidance of well-trained Lab-Personnel’s.</p>
+
  
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><b><u><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'><span style='mso-spacerun:yes'>       
 +
</span>(<span class=SpellE><span class=GramE>centre</span></span> of
 +
biosecurity at UNIVERSITY OF PITTSBURGH)<o:p></o:p></span></u></b></p>
  
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'><o:p>&nbsp;</o:p></span></p>
  
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>Biosafety refers to individual safeguards
 +
designed to protect laboratory personnel, as well as the surrounding environment
 +
and community. The biosafety protocols are divided <span class=SpellE>into&quot;BIOLOGICAL</span>
 +
SAFETY LEVELS (BSL), which are series of protection relegated to autoclave
 +
related activities that take place in particular labs.<o:p></o:p></span></p>
  
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>These levels are generally standardized
 +
across the world, however their implementation varies from country to country,
 +
labs to labs, organization to organizations.<o:p></o:p></span></p>
  
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>These biosafety levels are ranked from 1
 +
to 4, on the basis of agents or organisms that are being researched or worked
 +
on, in any given laboratory settings and are determined by the following-Risks
 +
related to containment, Severity of infection, Transmissibility, Nature of the
 +
work conducted, Origin of the microbe, Agent in <span class=SpellE>question,Route</span>
 +
of exposure<o:p></o:p></span></p>
  
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+
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</div><!--close content_imagetext-->
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none;text-autospace:none'><b style='mso-bidi-font-weight:normal'><u><span
−
<div class="content_imagetext1">
+
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman",serif'>BIOSAFETY
−
   
+
IN INDIA-<o:p></o:p></span></u></b></p>
−
 
+
−
  <div>
+
−
    <h2 id='htd'> <b>"WASTE MANAGEMENT FOR OUR WORK"</b></h2>
+
−
<p><b>Different trash containers are available in the lab:</b>One for biological waste (this waste will be autoclaved before being thrown out). Infection materials are also decontaminated prior to disposal, generally through the use of an autoclave.</p>
+
−
            <p>One for common waste, special container for chemicals, Special container for waste broth and cultures </p>
+
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</div>
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</div><!--close content_imagetext-->
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+
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</div><!-------close TEAM INSTRUCTORS-->
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+
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  <div>
+
−
    <h2 id='htd'> <b>BASIC RULES FOLLOWED IN OUR LABORATORY</b></h2>
+
−
<p>In the Biosafety level 1 laboratory, personnel is allowed to work with low risk microbes that pose little or no threat of infection in healthy adults. Under BSL-1 following practices are permitted-</p>
+
−
    <ul>
+
−
<li>Mechanical pipetting only (no mouth pipetting allowed)</li>
+
−
            <li>Safe sharps handling</li>
+
−
              <li>Avoidance of splashes or aerosols</li>
+
−
                <li>Daily decontamination of all work surfaces when work is complete</li>
+
−
                <li>Hand washing</li>
+
−
                <li>Prohibition of food, drink and smoking materials in lab setting</li>
+
−
<li>Personal protective equipment, such as; eye protection, gloves and a lab coat or gown</li>
+
−
<li>Biohazard signs</li>
+
−
            </ul>
+
−
<p>Apart from following the basic guidelines, our team takes security and safety measures at the highest priority. All members had to wear clean Lab Coat during the practical sessions. Team members had to wear lab sleepers, glove, goggles and mask according to demand of work. We also ensure proper disposal of gloves, used pipette tips, Eppendorf tubes in a separate bio hazardous container.</p>
+
−
<p>IGEM 2017 -TEAM DEI ensures all the protocols and safety measures are followed and implemented during team's lab work.</p>
+
−
</div>
+
−
 
+
−
 
+
−
 
+
−
 
+
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+
−
 
+
−
 
+
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+
  
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>IN India, a three tier mechanism
 +
comprising <b>Institutional</b> <b>Biosafety</b> <b>Committees</b> (<b>IBSC</b>)
 +
at the Institute/ company; the <b>Review</b> <b>Committee</b> <b>on</b> <b>Genetic</b>
 +
<b>Manipulation</b> (<b>RCGM</b>) in the Department of Biotechnology; and the <b>Genetic</b>
 +
<b>Engineering</b> <b>Approval</b> <b>Committee</b> (<b>GEAC</b>) in the
 +
Ministry of Environment &amp; Forests (<span class=SpellE>MoE&amp;F</span>), do
 +
analysis for granting approval for research and development activities on
 +
recombinant DNA products, environmental release of genetically engineered
 +
(GE)crops and monitoring and evaluation of research activities involving
 +
recombinant DNA technology has been established.<o:p></o:p></span></p>
  
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+
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none;text-autospace:none'><span style='font-size:12.0pt;line-height:150%;
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+
font-family:"Times New Roman",serif'><span style='mso-spacerun:yes'> </span>In
−
  </div><!--close main-->
+
India, GMOs and products thereof are regulated as per the “Rules for the manufacture,
−
 
+
use/import/export and storage of hazardous microorganisms/ genetically engineered
−
    <footer>
+
organisms or cells, 1989” (<span class=GramE>commonly</span> referred as Rules,
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1989) notified by the Ministry of Environment and Forests (<span class=SpellE>MoEF</span>),
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Government of India under the Environment (Protection) Act (1986). These rules
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are implemented by <span class=SpellE>MoEF</span>, the Department of
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Biotechnology (DBT), Ministry of Science and Technology and the State
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  <script type="text/javascript" src="js/jquery.js"></script>
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Governments through the six competent authorities notified under the Rules which
−
  <script type="text/javascript" src="js/image_slide.js"></script>
+
are as follows:<o:p></o:p></span></p>
−
 
+
 
 +
<p class=MsoListParagraphCxSpFirst style='margin-top:0in;margin-right:0in;
 +
margin-bottom:10.0pt;margin-left:.75in;mso-add-space:auto;text-align:justify;
 +
text-justify:inter-ideograph;text-indent:-.5in;line-height:150%;mso-pagination:
 +
none;mso-list:l3 level1 lfo3;mso-layout-grid-align:none;text-autospace:none'><![if !supportLists]><span
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman",serif;
 +
mso-fareast-font-family:"Times New Roman"'><span style='mso-list:Ignore'>i.<span
 +
style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>Recombinant DNA Advisory Committee (RDAC)<o:p></o:p></span></p>
 +
 
 +
<p class=MsoListParagraphCxSpMiddle style='margin-top:0in;margin-right:0in;
 +
margin-bottom:10.0pt;margin-left:.75in;mso-add-space:auto;text-align:justify;
 +
text-justify:inter-ideograph;text-indent:-.5in;line-height:150%;mso-pagination:
 +
none;mso-list:l3 level1 lfo3;mso-layout-grid-align:none;text-autospace:none'><![if !supportLists]><span
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman",serif;
 +
mso-fareast-font-family:"Times New Roman"'><span style='mso-list:Ignore'>ii.<span
 +
style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>Institutional Biosafety Committee (IBSC)<o:p></o:p></span></p>
 +
 
 +
<p class=MsoListParagraphCxSpMiddle style='margin-top:0in;margin-right:0in;
 +
margin-bottom:10.0pt;margin-left:.75in;mso-add-space:auto;text-align:justify;
 +
text-justify:inter-ideograph;text-indent:-.5in;line-height:150%;mso-pagination:
 +
none;mso-list:l3 level1 lfo3;mso-layout-grid-align:none;text-autospace:none'><![if !supportLists]><span
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman",serif;
 +
mso-fareast-font-family:"Times New Roman"'><span style='mso-list:Ignore'>iii.<span
 +
style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>Review Committee on Genetic Manipulation
 +
(RCGM)<o:p></o:p></span></p>
 +
 
 +
<p class=MsoListParagraphCxSpMiddle style='margin-top:0in;margin-right:0in;
 +
margin-bottom:10.0pt;margin-left:.75in;mso-add-space:auto;text-align:justify;
 +
text-justify:inter-ideograph;text-indent:-.5in;line-height:150%;mso-pagination:
 +
none;mso-list:l3 level1 lfo3;mso-layout-grid-align:none;text-autospace:none'><![if !supportLists]><span
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman",serif;
 +
mso-fareast-font-family:"Times New Roman"'><span style='mso-list:Ignore'>iv.<span
 +
style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>Genetic Engineering Appraisal Committee
 +
(GEAC)<o:p></o:p></span></p>
 +
 
 +
<p class=MsoListParagraphCxSpMiddle style='margin-top:0in;margin-right:0in;
 +
margin-bottom:10.0pt;margin-left:.75in;mso-add-space:auto;text-align:justify;
 +
text-justify:inter-ideograph;text-indent:-.5in;line-height:150%;mso-pagination:
 +
none;mso-list:l3 level1 lfo3;mso-layout-grid-align:none;text-autospace:none'><![if !supportLists]><span
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman",serif;
 +
mso-fareast-font-family:"Times New Roman"'><span style='mso-list:Ignore'>v.<span
 +
style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>State Biotechnology Coordination Committee
 +
(SBCC)<o:p></o:p></span></p>
 +
 
 +
<p class=MsoListParagraphCxSpLast style='margin-top:0in;margin-right:0in;
 +
margin-bottom:10.0pt;margin-left:.75in;mso-add-space:auto;text-align:justify;
 +
text-justify:inter-ideograph;text-indent:-.5in;line-height:150%;mso-pagination:
 +
none;mso-list:l3 level1 lfo3;mso-layout-grid-align:none;text-autospace:none'><![if !supportLists]><span
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman",serif;
 +
mso-fareast-font-family:"Times New Roman"'><span style='mso-list:Ignore'>vi.<span
 +
style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>District Level Committee (DLC).<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>While the RDAC has advisory in function,
 +
IBSC, RCGM, and GEAC<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
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none;text-autospace:none'><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'><span style='mso-spacerun:yes'> </span><b><u><span
 +
style='text-transform:uppercase'>Following steps are involved in the analysis
 +
of a Genetically Modified Cell -<o:p></o:p></span></u></b></span></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
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inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
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none;text-autospace:none'><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'><span
 +
style='mso-spacerun:yes'>                </span><span style='mso-no-proof:yes'><v:shapetype
 +
id="_x0000_t75" coordsize="21600,21600" o:spt="75" o:preferrelative="t"
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path="m@4@5l@4@11@9@11@9@5xe" filled="f" stroked="f">
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<v:stroke joinstyle="miter"/>
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<v:formulas>
 +
  <v:f eqn="if lineDrawn pixelLineWidth 0"/>
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  <v:f eqn="sum @0 1 0"/>
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</v:formulas>
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<v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"/>
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<o:lock v:ext="edit" aspectratio="t"/>
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</v:shapetype><v:shape id="Picture_x0020_2" o:spid="_x0000_i1025" type="#_x0000_t75"
 +
style='width:386.25pt;height:257.25pt;visibility:visible;mso-wrap-style:square'>
 +
<v:imagedata src="https://static.igem.org/mediawiki/2017/thumb/f/f3/Biosafety_01.jpg/120px-Biosafety_01.jpg" o:title="Slide1" croptop="7522f"
 +
  cropbottom="2961f" cropright="3601f"/>
 +
</v:shape></span><o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><b style='mso-bidi-font-weight:normal'><u><span
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman",serif'><o:p><span
 +
style='text-decoration:none'>&nbsp;</span></o:p></span></u></b></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><b style='mso-bidi-font-weight:normal'><u><span
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman",serif'><o:p><span
 +
style='text-decoration:none'>&nbsp;</span></o:p></span></u></b></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><b style='mso-bidi-font-weight:normal'><u><span
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman",serif'><o:p><span
 +
style='text-decoration:none'>&nbsp;</span></o:p></span></u></b></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><b style='mso-bidi-font-weight:normal'><u><span
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman",serif'>BIOSAFETY
 +
AT DAYALBAGH EDUCATIONAL INSTITUTE-<o:p></o:p></span></u></b></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>We at <span class=SpellE>Dayalbagh</span>
 +
Educational Institute understand the importance of Biosafety and strongly believe
 +
in its enforcement, therefore our lab works and experiments are carried out
 +
under strict biosafety precautions.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>Before commencing our wet lab experiments
 +
we had received a training sessions of two days on safety and precautions to be
 +
maintained while handling the Instruments, glassware’s <span class=GramE>and<span
 +
style='mso-spacerun:yes'>  </span>reagents</span> in a laboratory. <o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>For the iGEM project we are working with
 +
the <i style='mso-bidi-font-style:normal'><u>E</u>.<u>coli</u></i><u> </u>strain
 +
(DH5 &#945; and BL-<span class=GramE>21 )</span>, which falls under
 +
BSL-1(Biosafety level 1). Therefore all BSL-1 are implemented and followed
 +
during our laboratory workings<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><b style='mso-bidi-font-weight:normal'><u><span
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman",serif;
 +
text-transform:uppercase'>Sterilization:</span></u></b><span style='font-size:
 +
12.0pt;line-height:150%;font-family:"Times New Roman",serif'> Clean, Dirt free
 +
environment is essential for getting good results. We used sanitizers and 70%
 +
ethanol for cleaning the platform. <o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><b style='mso-bidi-font-weight:normal'><u><span
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman",serif;
 +
text-transform:uppercase'>Toxicity Risk:</span></u></b><span style='font-size:
 +
12.0pt;line-height:150%;font-family:"Times New Roman",serif'> Ethidium Bromide
 +
is the most commonly used staining dye for DNA, is highly toxic as mutagens. It
 +
may potentially cause carcinogenic effects. So for our team project we had
 +
decided to use a less toxic dye “GEL RED” purchased from GCC biotech.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><b style='mso-bidi-font-weight:normal'><u><span
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman",serif;
 +
text-transform:uppercase'>Handling of equipment:</span></u></b><span
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman",serif'>
 +
We used all necessary equipment’s such as Autoclave, U-V trans-illuminator, Biosafety
 +
Cabinet under the guidance of well-trained Lab-Personnel’s.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><b><u><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>WASTE MANAGEMENT FOR OUR WORK-<o:p></o:p></span></u></b></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>Different trash containers are available
 +
in the lab:<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>One for biological waste (this waste will
 +
be autoclaved before being thrown out). Infection materials are also
 +
decontaminated prior to disposal, generally through the use of an autoclave.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>One for common waste, special container
 +
for chemicals, Special container for waste broth and cultures<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span class=GramE><b style='mso-bidi-font-weight:
 +
normal'><u><span style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman",serif'>BASIC<span
 +
style='mso-spacerun:yes'>  </span>RULES</span></u></b></span><b
 +
style='mso-bidi-font-weight:normal'><u><span style='font-size:12.0pt;
 +
line-height:150%;font-family:"Times New Roman",serif'><span
 +
style='mso-spacerun:yes'>  </span>FOLLOWED IN OUR LABORATORY: <o:p></o:p></span></u></b></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
 +
none;text-autospace:none'><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>In the Biosafety level 1 laboratory,
 +
personnel is allowed to work with low risk microbes that pose little or no
 +
threat of infection in healthy adults. Under BSL-1 following practices are
 +
permitted-<o:p></o:p></span></p>
 +
 
 +
<p class=MsoListParagraph style='margin-bottom:10.0pt;mso-add-space:auto;
 +
text-align:justify;text-justify:inter-ideograph;text-indent:-.25in;line-height:
 +
150%;mso-pagination:none;mso-list:l2 level1 lfo6;mso-layout-grid-align:none;
 +
text-autospace:none'><![if !supportLists]><span style='font-size:12.0pt;
 +
line-height:150%;font-family:Symbol;mso-fareast-font-family:Symbol;mso-bidi-font-family:
 +
Symbol'><span style='mso-list:Ignore'>·<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>Mechanical pipetting only (no mouth
 +
pipetting allowed)<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-top:0in;margin-right:0in;margin-bottom:10.0pt;
 +
margin-left:.5in;text-align:justify;text-justify:inter-ideograph;text-indent:
 +
-.25in;line-height:150%;mso-pagination:none;mso-list:l0 level1 lfo1;mso-layout-grid-align:
 +
none;text-autospace:none'><![if !supportLists]><span style='font-size:12.0pt;
 +
line-height:150%;font-family:Symbol;mso-fareast-font-family:Symbol;mso-bidi-font-family:
 +
Symbol'><span style='mso-list:Ignore'>·<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>Safe sharps handling<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-top:0in;margin-right:0in;margin-bottom:10.0pt;
 +
margin-left:.5in;text-align:justify;text-justify:inter-ideograph;text-indent:
 +
-.25in;line-height:150%;mso-pagination:none;mso-list:l0 level1 lfo1;mso-layout-grid-align:
 +
none;text-autospace:none'><![if !supportLists]><span style='font-size:12.0pt;
 +
line-height:150%;font-family:Symbol;mso-fareast-font-family:Symbol;mso-bidi-font-family:
 +
Symbol'><span style='mso-list:Ignore'>·<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>Avoidance of splashes or aerosols<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-top:0in;margin-right:0in;margin-bottom:10.0pt;
 +
margin-left:.5in;text-align:justify;text-justify:inter-ideograph;text-indent:
 +
-.25in;line-height:150%;mso-pagination:none;mso-list:l0 level1 lfo1;mso-layout-grid-align:
 +
none;text-autospace:none'><![if !supportLists]><span style='font-size:12.0pt;
 +
line-height:150%;font-family:Symbol;mso-fareast-font-family:Symbol;mso-bidi-font-family:
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Symbol'><span style='mso-list:Ignore'>·<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>Daily decontamination of all work surfaces
 +
when work is complete<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-top:0in;margin-right:0in;margin-bottom:10.0pt;
 +
margin-left:.5in;text-align:justify;text-justify:inter-ideograph;text-indent:
 +
-.25in;line-height:150%;mso-pagination:none;mso-list:l0 level1 lfo1;mso-layout-grid-align:
 +
none;text-autospace:none'><![if !supportLists]><span style='font-size:12.0pt;
 +
line-height:150%;font-family:Symbol;mso-fareast-font-family:Symbol;mso-bidi-font-family:
 +
Symbol'><span style='mso-list:Ignore'>·<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>Hand washing<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-top:0in;margin-right:0in;margin-bottom:10.0pt;
 +
margin-left:.5in;text-align:justify;text-justify:inter-ideograph;text-indent:
 +
-.25in;line-height:150%;mso-pagination:none;mso-list:l0 level1 lfo1;mso-layout-grid-align:
 +
none;text-autospace:none'><![if !supportLists]><span style='font-size:12.0pt;
 +
line-height:150%;font-family:Symbol;mso-fareast-font-family:Symbol;mso-bidi-font-family:
 +
Symbol'><span style='mso-list:Ignore'>·<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>Prohibition of food, drink and smoking
 +
materials in lab setting<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-top:0in;margin-right:0in;margin-bottom:10.0pt;
 +
margin-left:.5in;text-align:justify;text-justify:inter-ideograph;text-indent:
 +
-.25in;line-height:150%;mso-pagination:none;mso-list:l0 level1 lfo1;mso-layout-grid-align:
 +
none;text-autospace:none'><![if !supportLists]><span style='font-size:12.0pt;
 +
line-height:150%;font-family:Symbol;mso-fareast-font-family:Symbol;mso-bidi-font-family:
 +
Symbol'><span style='mso-list:Ignore'>·<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>Personal protective equipment, such as;
 +
eye protection, gloves and a lab coat or gown<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-top:0in;margin-right:0in;margin-bottom:10.0pt;
 +
margin-left:.5in;text-align:justify;text-justify:inter-ideograph;text-indent:
 +
-.25in;line-height:150%;mso-pagination:none;mso-list:l0 level1 lfo1;mso-layout-grid-align:
 +
none;text-autospace:none'><![if !supportLists]><span style='font-size:12.0pt;
 +
line-height:150%;font-family:Symbol;mso-fareast-font-family:Symbol;mso-bidi-font-family:
 +
Symbol'><span style='mso-list:Ignore'>·<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 +
</span></span></span><![endif]><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>Biohazard signs<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
 +
inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
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none;text-autospace:none'><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'>Apart from following the basic guidelines,
 +
our team takes security and safety measures at the highest priority. All
 +
members had to wear clean Lab Coat during the practical sessions. Team members
 +
had to wear lab sleepers, glove, goggles and mask according to demand of work.
 +
We also ensure proper disposal of gloves, used pipette tips, Eppendorf tubes in
 +
a separate bio hazardous container.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
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inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
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none;text-autospace:none'><b style='mso-bidi-font-weight:normal'><span
 +
style='font-size:12.0pt;line-height:150%;font-family:"Times New Roman",serif'>IGEM
 +
2017 -TEAM DEI</span></b><span style='font-size:12.0pt;line-height:150%;
 +
font-family:"Times New Roman",serif'> ensures all the protocols and safety measures
 +
are followed and implemented during team's lab work.<o:p></o:p></span></p>
 +
 
 +
<p class=MsoNormal style='margin-bottom:10.0pt;text-align:justify;text-justify:
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inter-ideograph;line-height:150%;mso-pagination:none;mso-layout-grid-align:
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font-family:"Times New Roman",serif'><o:p>&nbsp;</o:p></span></p>
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Latest revision as of 18:26, 1 November 2017



  "Small Mistakes in Biosafety could have terrible global consequences"

                                                             by- Thomas Inglesky

        (centre of biosecurity at UNIVERSITY OF PITTSBURGH)

 

Biosafety refers to individual safeguards designed to protect laboratory personnel, as well as the surrounding environment and community. The biosafety protocols are divided into"BIOLOGICAL SAFETY LEVELS (BSL), which are series of protection relegated to autoclave related activities that take place in particular labs.

These levels are generally standardized across the world, however their implementation varies from country to country, labs to labs, organization to organizations.

These biosafety levels are ranked from 1 to 4, on the basis of agents or organisms that are being researched or worked on, in any given laboratory settings and are determined by the following-Risks related to containment, Severity of infection, Transmissibility, Nature of the work conducted, Origin of the microbe, Agent in question,Route of exposure

BIOSAFETY IN INDIA-

IN India, a three tier mechanism comprising Institutional Biosafety Committees (IBSC) at the Institute/ company; the Review Committee on Genetic Manipulation (RCGM) in the Department of Biotechnology; and the Genetic Engineering Approval Committee (GEAC) in the Ministry of Environment & Forests (MoE&F), do analysis for granting approval for research and development activities on recombinant DNA products, environmental release of genetically engineered (GE)crops and monitoring and evaluation of research activities involving recombinant DNA technology has been established.

 In India, GMOs and products thereof are regulated as per the “Rules for the manufacture, use/import/export and storage of hazardous microorganisms/ genetically engineered organisms or cells, 1989” (commonly referred as Rules, 1989) notified by the Ministry of Environment and Forests (MoEF), Government of India under the Environment (Protection) Act (1986). These rules are implemented by MoEF, the Department of Biotechnology (DBT), Ministry of Science and Technology and the State Governments through the six competent authorities notified under the Rules which are as follows:

i.                    Recombinant DNA Advisory Committee (RDAC)

ii.                  Institutional Biosafety Committee (IBSC)

iii.                Review Committee on Genetic Manipulation (RCGM)

iv.                Genetic Engineering Appraisal Committee (GEAC)

v.                  State Biotechnology Coordination Committee (SBCC)

vi.                District Level Committee (DLC).

While the RDAC has advisory in function, IBSC, RCGM, and GEAC

 Following steps are involved in the analysis of a Genetically Modified Cell -

               

 

 

 

BIOSAFETY AT DAYALBAGH EDUCATIONAL INSTITUTE-

We at Dayalbagh Educational Institute understand the importance of Biosafety and strongly believe in its enforcement, therefore our lab works and experiments are carried out under strict biosafety precautions.

Before commencing our wet lab experiments we had received a training sessions of two days on safety and precautions to be maintained while handling the Instruments, glassware’s and  reagents in a laboratory.

For the iGEM project we are working with the E.coli strain (DH5 α and BL-21 ), which falls under BSL-1(Biosafety level 1). Therefore all BSL-1 are implemented and followed during our laboratory workings

Sterilization: Clean, Dirt free environment is essential for getting good results. We used sanitizers and 70% ethanol for cleaning the platform.

Toxicity Risk: Ethidium Bromide is the most commonly used staining dye for DNA, is highly toxic as mutagens. It may potentially cause carcinogenic effects. So for our team project we had decided to use a less toxic dye “GEL RED” purchased from GCC biotech.

Handling of equipment: We used all necessary equipment’s such as Autoclave, U-V trans-illuminator, Biosafety Cabinet under the guidance of well-trained Lab-Personnel’s.

WASTE MANAGEMENT FOR OUR WORK-

Different trash containers are available in the lab:

One for biological waste (this waste will be autoclaved before being thrown out). Infection materials are also decontaminated prior to disposal, generally through the use of an autoclave.

One for common waste, special container for chemicals, Special container for waste broth and cultures

BASIC  RULES  FOLLOWED IN OUR LABORATORY:

In the Biosafety level 1 laboratory, personnel is allowed to work with low risk microbes that pose little or no threat of infection in healthy adults. Under BSL-1 following practices are permitted-

·            Mechanical pipetting only (no mouth pipetting allowed)

·         Safe sharps handling

·         Avoidance of splashes or aerosols

·         Daily decontamination of all work surfaces when work is complete

·         Hand washing

·         Prohibition of food, drink and smoking materials in lab setting

·         Personal protective equipment, such as; eye protection, gloves and a lab coat or gown

·         Biohazard signs

Apart from following the basic guidelines, our team takes security and safety measures at the highest priority. All members had to wear clean Lab Coat during the practical sessions. Team members had to wear lab sleepers, glove, goggles and mask according to demand of work. We also ensure proper disposal of gloves, used pipette tips, Eppendorf tubes in a separate bio hazardous container.

IGEM 2017 -TEAM DEI ensures all the protocols and safety measures are followed and implemented during team's lab work.