Difference between revisions of "Template:Team:Utrecht/MainBody"

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<br>
 
<br>
 
<span class="text-figure">
 
<span class="text-figure">
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Attenting a RIVM event. <a href="javascript:void(0)" onclick="openmodal('rivm-event')" style="font-size: 11px;">Click for full size.</a>
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Attending a RIVM event. <a href="javascript:void(0)" onclick="openmodal('rivm-event')" style="font-size: 11px;">Click for full size.</a>
 
</span>
 
</span>
 
</center>
 
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</script>
 
</script>
  
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<script id="page-attributions" type="text/template">
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<script id="page-basic_part" type="text/template">
 
<div class="page-heading">BioBricks - Basic Parts</div>
 
<div class="page-heading">BioBricks - Basic Parts</div>
  
−
This page contains an overview of all the basic BioBricks we created for our project.
+
This page contains an overview of all the basic BioBricks we created for our project.<br>
 +
Interested in our composite parts? You can find them <a onclick="return change_page('composite_part', 1)" href="composite_part">here</a>.
 +
 
 +
<br><br>
 +
 
 +
<table class="biobricktable" style="width: 100%; font-size: 12px; text-align: left;">
 +
<tr style="font-size: 15px; font-weight: bold;">
 +
<td width="150">Number</td>
 +
<td width="200">Name</td>
 +
<td>Short description</td>
 +
</tr>
 +
<tr><td><a target=_BLANK href="http://parts.igem.org/Part:BBa_K2351000" class="url_external">BBa_K2351000</a></td><td>Ig lambda-2 chain V region signal sequence</td><td>Allows for proteins to be secreted</td></tr>
 +
<tr><td><a target=_BLANK href="http://parts.igem.org/Part:BBa_K2351001" class="url_external">BBa_K2351001</a></td><td><b>Best basic part:</b> dAsCpf1</td><td>DNA and gRNA binding properties are maintained but no endonuclease activity is exhibited.</td></tr>
 +
<tr><td><a target=_BLANK href="http://parts.igem.org/Part:BBa_K2351002" class="url_external">BBa_K2351002</a></td><td>nCas9 </td><td>A cas9 variant without first Methionine and without stopcodon, such that it can be used as a fusion protein component that contains illegal restriction sites.</td></tr>
 +
<tr><td><a target=_BLANK href="http://parts.igem.org/Part:BBa_K2351003" class="url_external">BBa_K2351003</a></td><td>nCpf1</td><td>A cpf1 variant without first Methionine and without stopcodon, such that it can be used as a fusion protein component that contains illegal restriction sites.</td></tr>
 +
<tr><td><a target=_BLANK href="http://parts.igem.org/Part:BBa_K2351004" class="url_external">BBa_K2351004</a></td><td>Glycine [3x] linked histidine[6x] tag</td><td>Basic histidine tag with preceding glycine linker.</td></tr>
 +
<tr><td><a target=_BLANK href="http://parts.igem.org/Part:BBa_K2351007" class="url_external">BBa_K2351007</a></td><td>nCas9 </td><td>A cas9 variant without first Methionine and without stopcodon, such that it can be used as a fusion protein component.</td></tr>
 +
<tr><td><a target=_BLANK href="http://parts.igem.org/Part:BBa_K2351008" class="url_external">BBa_K2351008</a></td><td>nCpf1</td><td>A cpf1 variant without first Methionine and without stopcodon, such that it can be used as a fusion protein component.</td></tr>
 +
<tr><td><a target=_BLANK href="http://parts.igem.org/Part:BBa_K23510011" class="url_external">BBa_K23510011</a></td><td>dCpf1</td><td>DNA and gRNA binding properties are maintained but no endonuclease activity is exhibited.</td></tr>
 +
<tr><td><a target=_BLANK href="http://parts.igem.org/Part:BBa_K23510012" class="url_external">BBa_K23510012</a></td><td>sCpf1</td><td>Phytobrick* // Cpf1 that will be secreted from humane cells.</td></tr>
 +
<tr><td><a target=_BLANK href="http://parts.igem.org/Part:BBa_K23510013" class="url_external">BBa_K23510013</a></td><td>dCpf1</td><td>Phytobrick*// DNA and gRNA binding properties are maintained but no endonuclease activity is exhibited.</td></tr>
 +
<tr><td><a target=_BLANK href="http://parts.igem.org/Part:BBa_K23510014" class="url_external">BBa_K23510014</a></td><td>sCas9</td><td>Phytobrick* // Cas9 that will be secreted from humane cells.</td></tr>
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</table>
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<style type="text/css">
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</style>
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</script>
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<script id="page-composite_part" type="text/template">
 +
<div class="page-heading">BioBricks - Composite Parts</div>
 +
 
 +
This page contains an overview of all the composite BioBricks we created for our project.<br>
 +
Interested in our basic parts? You can find them <a onclick="return change_page('basic_part', 1)" href="basic_part">here</a>.
  
 
<br><br>
 
<br><br>
  
−
<table>
+
<table class="biobricktable" style="width: 100%; font-size: 12px; text-align: left;">
−
<tr>
+
<tr style="font-size: 15px; font-weight: bold;">
−
<td>Number</td>
+
<td width="150">Number</td>
−
<td>Name</td>
+
<td width="200">Name</td>
 
<td>Short description</td>
 
<td>Short description</td>
 
</tr>
 
</tr>
−
<tr>
+
<tr><td><a target=_BLANK href="http://parts.igem.org/Part:BBa_K2351005" class="url_external">BBa_K2351005</a></td><td><b>Best composite part:</b> secreted Cas9</td><td>Cas9 that will be secreted from humane cells.</td></tr>
−
Ba_K2351000: parts.igem.org/Part:BBa_K2351000 Ig lambda-2 chain V region signal sequence Allows for proteins to be secreted
+
<tr><td><a target=_BLANK href="http://parts.igem.org/Part:BBa_K2351006" class="url_external">BBa_K2351006</a></td><td>secreted Cpf1</td><td>Cpf1 that will be secreted from humane cells.</td></tr>
−
Ba_K2351001
+
<tr><td><a target=_BLANK href="http://parts.igem.org/Part:BBa_K2351009" class="url_external">BBa_K2351009</a></td><td>sCas9</td><td>Cas9 that will be secreted from humane cells.</td></tr>
−
parts.igem.org/Part:BBa_K2351001 Best basic part: dAsCpf1 DNA and gRNA binding properties are maintained but no endonuclease activity is exhibited.
+
<tr><td><a target=_BLANK href="http://parts.igem.org/Part:BBa_K23510010" class="url_external">BBa_K23510010</a></td><td>sCpf1</td><td>Cpf1 that will be secreted from humane cells.</td></tr>
−
Ba_K2351002
+
</table>
−
parts.igem.org/Part:BBa_K2351002 nCas9 A cas9 variant without first Methionine and without stopcodon, such that it can be used as a fusion protein component that contains illegal restriction sites.
+
−
Ba_K2351003
+
−
parts.igem.org/Part:BBa_K2351003 nCpf1 A cpf1 variant without first Methionine and without stopcodon, such that it can be used as a fusion protein component that contains illegal restriction sites.
+
−
Ba_K2351004
+
−
parts.igem.org/Part:BBa_K2351004 Glycine [3x] linked histidine[6x] tag Basic histidine tag with preceding glycine linker
+
−
Ba_K2351007
+
−
parts.igem.org/Part:BBa_K2351007 nCas9 A cas9 variant without first Methionine and without stopcodon, such that it can be used as a fusion protein component.
+
−
Ba_K2351008
+
−
parts.igem.org/Part:BBa_K2351008 nCpf1 A cpf1 variant without first Methionine and without stopcodon, such that it can be used as a fusion protein component.
+
−
Ba_K23510011
+
−
parts.igem.org/Part:BBa_K2351011 dCpf1 DNA and gRNA binding properties are maintained but no endonuclease activity is exhibited.
+
−
Ba_K23510012
+
−
parts.igem.org/Part:BBa_K2351012 sCpf1 Phytobrick* // Cpf1 that will be secreted from humane cells.
+
−
Ba_K23510013
+
−
parts.igem.org/Part:BBa_K2351013 dCpf1 Phytobrick*// DNA and gRNA binding properties are maintained but no endonuclease activity is exhibited.
+
−
Ba_K23510014
+
−
parts.igem.org/Part:BBa_K2351014 sCas9 Phytobrick* // Cas9 that will be secreted from humane cells
+
  
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<style type="text/css">
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.biobricktable tr:nth-child(even) { background: #f7f7f7; }
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.biobricktable td { padding: 5px; }
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6: "Administrative assistance",
 
6: "Administrative assistance",
 
7: "Final word"
 
7: "Final word"
−
}
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},
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"basic_part" : {},
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"composite_part" : {}
 
};
 
};
  

Revision as of 19:07, 1 November 2017

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  • Home
  • Experimental
  • Human practices
  • Team & Sponsors
Cas9 & Cpf1 secretion
and activity
Comparison of endonuclease activity for Cas9 and Cpf1 that has been produced in, and excreted by, HEK293 cells.
MESA two-component system replication
Details on the MESA two-component system, explanation of its relation to our design and the results of its reproduction.
OUTCASST system production
Detailed explanation of the OUTCASST mechanism, experimental progress and technical prospects.
Modeling and
mathematics
Ordinary differential equations, cellular automaton and an object based model for optimal linker-length estimation.
InterLab study participation
Results and details of our measurements for the iGEM 2017 InterLab Study.
Stakeholders & opinions
Interviews and dialogues with stakeholders, potential users, third parties and experts relating to pathogen detection or DNA-based diagnostics.
Risks & safety-issues
Implications and design considerations relating to safety in the usage and implementation of OUTCASST as a diagnostics tool.
Design & integration
OUTCASST toolkit and product design with factors such as bio-safety and user-friendliness taken into account.
Outreach
Videos we made for the dutch public, together with 'de Kennis van Nu'.
Meet our team
About us, our interests and roles in the team and our supervisors.
Sponsors
A listing of our sponsors, how they assisted us and our gratitude for their assistance.
Collaborations
Read about our exchanges with other iGEM teams and government agencies.
Achievements
A short description of all that we have achieved during our participation in the iGEM.
Attributions
A thank-you for everyone that assited us, both in and outside the lab.
Intro
The problem
The system