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<h2 class="text-left" style="margin-top: 2%; margin-bottom: 2%; font-family: Rubik">Parts combined to form complete cell systems </h2> | <h2 class="text-left" style="margin-top: 2%; margin-bottom: 2%; font-family: Rubik">Parts combined to form complete cell systems </h2> | ||
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<b> Detector Cell </b></br> | <b> Detector Cell </b></br> | ||
IPTG Detector: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2205009"> BBa_K2205009 </a> </br> | IPTG Detector: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2205009"> BBa_K2205009 </a> </br> |
Latest revision as of 19:08, 1 November 2017
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Part Collection
Sensynova Framework parts
Part range BBa_K2205007 – BBa_K2205030
The Newcastle iGEM team has developed the Sensynova framework to ease biosensor development through the use of mixed cell populations interacting via intercellular communication molecules. Each cell type has a distinct function according a design-pattern determined through systematic review of 121 previous iGEM biosensor projects. The system is detailed in the figure below – The dotted orange lines correspond to the junction between parts.
A target molecule is detected by a protein constitutively expressed in our Detector cell. A promoter sensitive to this protein drives the expression of the lasI gene. The LasI protein catalyses the production of 3O-C12 N-acyl homoserine lactone (C12 AHL). C12 AHL diffuses out of the cell and interacts with LasR, which is constitutively expressed in the Processing cell. LasR activates the pLas promoter. In the system, the pLas promoter drives expression of the rhlI gene, producing the RhlI protein which catalysis the formation of our second connector 3O-C4 N-acyl homoserine lactone (C4 AHL). The C4 diffuses out of the cell and interacts with RhlR in the output cell. This complex activates the pRhl promoter which regulates the transcription of an output device, such as a fluorescence protein. Therefore, upon triggering a detection device in the detection cell, intercellular connectors are able to propagate this signal to cells containing processing and output devices. As the connectors used are standard to cell types, cell mixing, rather than genetic re-engineering, can be used to test future processing and output variants.
We have demonstrated that biosensors built using this framework are able to generate desired responses to input molecules. For more information, visit our results pages here .
Parts Domesticated for use with our framework
Detectors IPTG Detector: BBa_K2205007 Formaldehyde: BBa_K2205029 Detector Cell LasI connector BBa_K2205008 Processing Cell LasR connector BBa_K2205010 Processing Fim Standby Switch: BBa_K2205024 PSP3 pag signal tuner: BBa_K2205025 Processing Cell RhlI connector BBa_K2205011 Reporter cell RhlR connector BBa_K2205013 Reporter sfGFP: BBa_K2205014 deGFP: BBa_K2205007Parts combined to form complete cell systems
Detector Cell IPTG Detector: BBa_K2205009 Psicose: BBa_K2205023 Arsenic: BBa_K2205022 Formaldehyde: BBa_K2205030 Processing Cell Blank: BBa_K2205012 P2 ogr signal tuner: BBa_K2205024 P2 ogr signal tuner (connector 1B only): BBa_K2205027 PSP3 pag signal tuner: BBa_K2205025 PSP3 pag signal tuner (connector 1B only): BBa_K2205028 Fim Standby Switch: BBa_K2205021 Reporter Cell sfGFP: BBa_K2205015 aeBlue: BBa_K2205016 amajLime: BBa_K2205017 spisPink: BBa_K2205018 deGFP: BBa_K2205020![]() |
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