Difference between revisions of "Team:SJTU-BioX-Shanghai/Contribution"

 
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                            <li class="nav-item"><a href="https://2017.igem.org/Team:SJTU-BioX-Shanghai/Description" class="nav-link">Project</a></li>
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                            <li class="nav-item"><a href="https://2017.igem.org/Team:SJTU-BioX-Shanghai/Notebook" class="nav-link">Wet Lab</a></li>
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<h1>Contribution</h1>
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        <div class="big-head h1-my-responsive"> <img src="https://static.igem.org/mediawiki/2017/5/52/T--SJTU-BioX-Shanghai--quan.png" class="img-fluid">Contribution</div>
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<h3>Bronze Medal Criterion #4</h3>
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study (to be documented on your InterLab page) and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range. Teams who are working on improving the characterization of an existing part should document their experimental design here, along with an explanation for why they chose that part to improve. Data can also be shown here, but it MUST also be documented on the part's Main Page in the Registry.
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<b>Special Tracks:</b> Document at least one new substantial contribution to the iGEM community that showcases a project related to BioBricks. This contribution should be central to your project and equivalent in difficulty to making and submitting a BioBrick part.
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                        <p>This summer, we designed another STAR system to achieve multiple factors detection. To make these systems more convenient, we constructed both Target and STAR(called Antisense in our project) on the same plasmid. In order to know whether the characteristics change, we conducted the same experiment again but in our dual-expression plasmid pCDF-Duet1 based on the characterization above. To distinguish two systems, we call our new STAR system STAR3 and the STAR system used by Imperial College STAR1(Part:BBa_K1893013).</p>
  
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                            <div class="figure-text">Figure 1(A) (B) : Characterization of STARs system in DH5α strain with pCDF as vector. Nomalized fluorescence over time for cell growth incorporating Star1 system in the absence or presence of transcribed Antisense Molecules. We used the dual-expression plasmid pCDF-Duet1. For the absence of Antisense1, the plasmid just includes Target1 in the MCS1. For the presence of Antisense1, Antisense1 is inserted into MCS2. For control(background), only pCDF backbone is transformed into DH5α. </div>
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                    <a target="_blank" href="http://www.sjtu.edu.cn/">
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                    <a target="_blank" href="http://www.bio-x.cn/">
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                    <a target="_blank" href="http://life.sjtu.edu.cn/">
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                    <a target="_blank" href="http://www.decodinglife.cn/">
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Latest revision as of 19:25, 1 November 2017

Contribution

This summer, we designed another STAR system to achieve multiple factors detection. To make these systems more convenient, we constructed both Target and STAR(called Antisense in our project) on the same plasmid. In order to know whether the characteristics change, we conducted the same experiment again but in our dual-expression plasmid pCDF-Duet1 based on the characterization above. To distinguish two systems, we call our new STAR system STAR3 and the STAR system used by Imperial College STAR1(Part:BBa_K1893013).

Figure 1(A) (B) : Characterization of STARs system in DH5α strain with pCDF as vector. Nomalized fluorescence over time for cell growth incorporating Star1 system in the absence or presence of transcribed Antisense Molecules. We used the dual-expression plasmid pCDF-Duet1. For the absence of Antisense1, the plasmid just includes Target1 in the MCS1. For the presence of Antisense1, Antisense1 is inserted into MCS2. For control(background), only pCDF backbone is transformed into DH5α.