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| − | <head>
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| − | <title></title>
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| − | </head>
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| − | <body>
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| − | <!-- Junio -->
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| − | <div id="date-6-1">
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| − | <h1>Sunday, June 1st.</h1>
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| − | </div>
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| − |
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| − | <div id="date-6-2">
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| − | <h1>Monday, June 2nd.</h1>
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| − | <ul>
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| − | <li>Lab cleanup, 12:00.</li>
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| − | <ul>
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| − | <li>Everyone.</li>
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| − | </ul>
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| − | </ul>
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| − | </div>
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| − |
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| − | <div id="date-6-3">
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| − | <h1>Tuesday, June 3rd.</h1>
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| − | </div>
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| − |
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| − | <div id="date-6-4">
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| − | <h1>Wednesday, June 5th.</h1>
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| − | </div>
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| − |
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| − | <div id="date-6-5">
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| − | <h1>Thursday, June 5th.</h1>
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| − | </div>
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| − |
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| − | <div id="date-6-6">
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| − | <h1>Friday, June 6th.</h1>
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| − | <ul>
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| − | <li>iGEM 2014 meeting to talk about lab work, 14:00.</li>
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| − | <ul>
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| − | <li>Everyone.</li>
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| − | </ul>
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| − | </ul>
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| − | </div>
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| − |
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| − | <div id="date-6-7">
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| − | <h1>Saturday, June 7th.</h1>
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| − | </div>
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| − |
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| − | <div id="date-6-8">
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| − | <h1>Sunday, June 8th.</h1>
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| − | </div>
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| − |
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| − | <div id="date-6-9">
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| − | <h1>Monday, June 9th.</h1>
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| − | </div>
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| − |
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| − | <div id="date-6-10">
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| − | <h1>Tuesday, June 10th.</h1>
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| − | <ul>
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| − | <li>Revision of the protocols for next month’s lab-work, 12:00.</li>
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| − | <ul>
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| − | <li>Everyone.</li>
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| − | </ul>
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| − | <li>Revision of human practice ideas and collaborations with other teams, 13:00.</li>
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| − | <ul>
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| − | <li>Everyone.</li>
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| − | </ul>
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| − | </ul>
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| − | </div>
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| − |
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| − | <div id="date-6-11">
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| − | <h1>Wednesday, June 11th.</h1>
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| − | <ul>
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| − | <li>Electro-competent cells, 13:00.</li>
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| − | <ul>
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| − | <li>Cinthya</li>
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| − | </ul>
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| − | </ul>
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| − | </div>
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| − |
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| − | <div id="date-6-12">
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| − | <h1>Thursday, June 12th.</h1>
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| − | <ul>
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| − | <li>Sponsorships, all day.</li>
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| − | <ul>
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| − | <li>Cinthya.</li>
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| − | </ul>
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| − | </ul>
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| − | </div>
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| − |
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| − | <div id="date-6-13">
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| − | <h1>Friday, June 13th.</h1>
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| − | <ul>
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| − | <li>Meeting with advisors, 17:00.</li>
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| − | <li>Calcium-competent cells, 13:00.</li>
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| − | <ul>
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| − | <li>Cinthya.</li>
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| − | </ul>
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| − | </ul>
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| − | </div>
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| − |
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| − | <div id="date-6-14">
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| − | <h1>Saturday, June 14th.</h1>
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| − | </div>
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| − |
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| − | <div id="date-6-15">
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| − | <h1>Sunday, June 15th.</h1>
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| − | </div>
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| − |
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| − | <div id="date-6-16">
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| − | <h1>Monday, June 16th.</h1>
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| − | <ul>
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| − | <li>Electro-competent cells, 14:00.</li>
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| − | <ul>
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| − | <li>Cinthya, Mercedes.</li>
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| − | </ul>
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| − | <li>Minipreps, 16:00.</li>
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| − | <ul>
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| − | <li>Cinthya.</li>
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| − | </ul>
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| − | <li>Electrophoresis, 19:00.</li>
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| − | <ul>
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| − | <li>Cinthya.</li>
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| − | </ul>
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| − | <li>Inoculations, 20:00.</li>
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| − | <ul>
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| − | <li>Mercedes.</li>
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| − | </ul>
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| − | </ul>
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| − | </div>
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| − |
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| − | <div id="date-6-17">
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| − | <h1>Tuesday, June 17th.</h1>
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| − | </div>
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| − |
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| − | <div id="date-6-18">
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| − | <h1>Wednesday, June 18th.</h1>
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| − | <ul>
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| − | <li>Electro-competent cells, 10:00.</li>
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| − | <ul>
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| − | <li>Cinthya.</li>
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| − | </ul>
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| − | <li>Minipreps, 13:00.</li>
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| − | <ul>
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| − | <li>Mercedes.</li>
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| − | </ul>
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| − | <li>Electrophoresis, 16:00.</li>
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| − | <ul>
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| − | <li>Cinthya.</li>
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| − | </ul>
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| − | <li>Inoculations, 20:00.</li>
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| − | <ul>
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| − | <li>Cinthya.</li>
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| − | </ul>
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| − | </ul>
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| − | </div>
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| − |
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| − | <div id="date-6-19">
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| − | <h1>Thursday, June 19th.</h1>
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| − | </div>
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| − |
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| − | <div id="date-6-20">
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| − | <h1>Friday, June 20th.</h1>
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| − | <ul>
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| − | <li>Meeting with advisors, 17:00.</li>
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| − | </ul>
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| − | </div>
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| − |
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| − | <div id="date-6-21">
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| − | <h1>Saturday, June 21st.</h1>
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| − | </div>
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| − |
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| − | <div id="date-6-22">
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| − | <h1>Sunday, June 22nd.</h1>
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| − | </div>
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| − |
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| − | <div id="date-6-23">
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| − | <h1>Monday, June 23rd.</h1>
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| − | <ul>
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| − | <li>Calcium-competent cells, 12:00.</li>
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| − | <ul>
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| − | <li>Cinthya.</li>
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| − | </ul>
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| − | </ul>
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| − | </div>
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| − |
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| − | <div id="date-6-24">
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| − | <h1>Tuesday, June 24th.</h1>
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| − | <ul>
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| − | <li>Minipreps, 11:00</li>
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| − | <ul>
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| − | <li>Mercedes.</li>
| |
| − | </ul>
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| − | <li>Electrophoresis, 14:00.</li>
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| − | <ul>
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| − | <li>Mercedes.</li>
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| − | </ul>
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| − | <li>Inoculations. 18:00.</li>
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| − | <ul>
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| − | <li>Mercedes.</li>
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| − | </ul>
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| − | </ul>
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| − | </div>
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| − |
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| − | <div id="date-6-25">
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| − | <h1>Wednesday, June 25nd.</h1>
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| − | </div>
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| − |
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| − | <div id="date-6-26">
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| − | <h1>Thursday, June 26th.</h1>
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| − | <ul>
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| − | <li>Minipreps, 10:00</li>
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| − | <ul>
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| − | <li>Mercedes.</li>
| |
| − | </ul>
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| − | <li>Electrophoresis, 13:30.</li>
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| − | <ul>
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| − | <li>Mercedes.</li>
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| − | </ul>
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| − | </ul>
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| − | </div>
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| − |
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| − | <div id="date-6-27">
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| − | <h1>Friday, June 27th.</h1>
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| − | <ul>
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| − | <li>Meeting with advisors, 17:00.</li>
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| − | </ul>
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| − | </div>
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| − |
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| − | <div id="date-6-28">
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| − | <h1>Saturday, June 28nd.</h1>
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| − | </div>
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| − |
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| − | <div id="date-6-29">
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| − | <h1>Sunday, June 29nd.</h1>
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| − | </div>
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| − |
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| − | <div id="date-6-30">
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| − | <h1>Monday, June 30nd.</h1>
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| − | </div>
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| − |
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| − |
| |
| − | <!-- Julio -->
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| − | <div id="date-7-1">
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| − | <h1>Tuesday, July 1st.</h1>
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| − | <ul>
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| − | <li>Practice: Minipreps, 13:00.</li>
| |
| − | <ul>
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| − | <li>Eduardo Ramirez (1).</li>
| |
| − | </ul>
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| − | <li>Practice: Ligations, 16:00.</li>
| |
| − | <ul>
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| − | <li>Eduardo Ramirez (1).</li>
| |
| − | </ul>
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| − | </ul>
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| − | </div>
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| − |
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| − | <div id="date-7-2">
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| − | <h1>Wednesday, July 2nd.</h1>
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| − | </div>
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| − |
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| − | <div id="date-7-3">
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| − | <h1>Thursday, July 3rd.</h1>
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| − | </div>
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| − |
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| − | <div id="date-7-4">
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| − | <h1>Friday, July 4th.</h1>
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| − | <ul>
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| − | <li>Minipreps, 12:00.</li>
| |
| − | <ul>
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| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Practice: Ligations, 15:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Ramirez (1).</li>
| |
| − | </ul>
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| − | <li>Practice: Electrophoresis, 17:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Ramirez (1)</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
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| − |
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| − | <div id="date-7-5">
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| − | <h1>Saturday, July 5th.</h1>
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| − | </div>
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| − |
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| − | <div id="date-7-6">
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| − | <h1>Sunday, July 6th.</h1>
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| − | </div>
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| − |
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| − | <div id="date-7-7">
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| − | <h1>Monday, July 7th.</h1>
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| − | <ul>
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| − | <li>Sendoffs of our teammates for a deserved vacation through the next 2 weeks.</li>
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| − | <li>Practice: Minipreps, 16:00.</li>
| |
| − | <ul>
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| − | <li>Claudia.</li>
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| − | </ul>
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| − | <li>Omar leaves for vacation.</li>
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| − | </ul>
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| − | </div>
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| − |
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| − | <div id="date-7-8">
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| − | <h1>Tuesday, July 8th.</h1>
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| − | </div>
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| − |
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| − | <div id="date-7-9">
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| − | <h1>Wednesday, July 9th.</h1>
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| − | </div>
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| − |
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| − | <div id="date-7-10">
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| − | <h1>Thursday, July 10th.</h1>
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| − | <ul>
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| − | <li>Practice: Transformation of calcium-competent cells, 11:00,</li>
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| − | <ul>
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| − | <li>Eduardo Ramirez (1), Claudia.</li>
| |
| − | </ul>
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| − | <li>Inoculation on LB-agar plates, 13:00.</li>
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| − | <ul>
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| − | <li>Claudia.</li>
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| − | </ul>
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| − | <li>Laboratory summer cleaning, 15:00.</li>
| |
| − | <ul>
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| − | <li>Claudia.</li>
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| − | </ul>
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| − | </ul>
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| − | </div>
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| − |
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| − | <div id="date-7-11">
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| − | <h1>Friday, July 11th.</h1>
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| − | </div>
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| − |
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| − | <div id="date-7-12">
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| − | <h1>Saturday, July 12th.</h1>
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| − | </div>
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| − |
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| − | <div id="date-7-13">
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| − | <h1>Sunday, July 13th.</h1>
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| − | </div>
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| − |
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| − | <div id="date-7-14">
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| − | <h1>Monday, July 14th.</h1>
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| − | </div>
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| − |
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| − | <div id="date-7-15">
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| − | <h1>Tuesday, July 15th.</h1>
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| − | <ul>
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| − | <li>Practice: taking DNA out of the iGEM plates, 13:00.</li>
| |
| − | <ul>
| |
| − | <li>Claudia.</li>
| |
| − | </ul>
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| − | <li>Transformation with calcium-competent cells, 13:30.</li>
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| − | <ul>
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| − | <li>Claudia.</li>
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| − | </ul>
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| − | <li>Inoculations, 15:00.</li>
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| − | <ul>
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| − | <li>Claudia.</li>
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| − | </ul>
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| − | </ul>
| |
| − | </div>
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| − |
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| − | <div id="date-7-16">
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| − | <h1>Wednesday, July 16th.</h1>
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| − | <ul>
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| − | <li>Minipreps, 12:00.</li>
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| − | <ul>
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| − | <li>Eduardo Ramirez (1).</li>
| |
| − | </ul>
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| − | <li>Ligations, 15:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Ramirez (1).</li>
| |
| − | </ul>
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| − | <li>Electrophoresis, 18:00.</li>
| |
| − | <li>Practice: DNA recovery from gel, 20:00.</li>
| |
| − | <ul>
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| − | <li>Eduardo Ramirez (1)</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
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| − |
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| − | <div id="date-7-17">
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| − | <h1>Thursday, July 17th.</h1>
| |
| − | </div>
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| − |
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| − | <div id="date-7-18">
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| − | <h1>Friday, July 18th.</h1>
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| − | </div>
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| − |
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| − | <div id="date-7-19">
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| − | <h1>Saturday, July 19th.</h1>
| |
| − | <ul>
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| − | <li>Meeting with advisors to talk about gene problems and doubts, 17:00.</li>
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| − | </ul>
| |
| − | </div>
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| − |
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| − | <div id="date-7-20">
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| − | <h1>Sunday, July 20th.</h1>
| |
| − | </div>
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| − |
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| − | <div id="date-7-21">
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| − | <h1>Monday, July 21st.</h1>
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| − | </div>
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| − |
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| − | <div id="date-7-22">
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| − | <h1>Tuesday, July 22nd.</h1>
| |
| − | <ul>
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| − | <li>Minipreps, 11:00.</li>
| |
| − | <ul>
| |
| − | <li>Claudia.</li>
| |
| − | </ul>
| |
| − | <li>Ligations, 15:00.</li>
| |
| − | <ul>
| |
| − | <li>Claudia.</li>
| |
| − | </ul>
| |
| − | <li>Electrophoresis, 19:00.</li>
| |
| − | <ul>
| |
| − | <li>Claudia</li>
| |
| − | </ul>
| |
| − | <li>Practice: DNA recovery from gel, 21:00.</li>
| |
| − | <ul>
| |
| − | <li>Claudia.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
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| − | <div id="date-7-23">
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| − | <h1>Wednesday, July 23rd.</h1>
| |
| − | </div>
| |
| − |
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| − | <div id="date-7-24">
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| − | <h1>Thursday, July 24th.</h1>
| |
| − | </div>
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| − |
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| − | <div id="date-7-25">
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| − | <h1>Friday, July 25th.</h1>
| |
| − | </div>
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| − |
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| − | <div id="date-7-26">
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| − | <h1>Saturday, July 26th.</h1>
| |
| − | <ul>
| |
| − | <li>Meeting with advisors, 17:00.</li>
| |
| − | </ul>
| |
| − | <h1><strong><strong> </strong></strong></h1>
| |
| − | <h1>Monday, July 29th.</h1>
| |
| − | <ul>
| |
| − | <li>Meeting to plan the semester’s activities, 14:00.</li>
| |
| − | <ul>
| |
| − | <li>Schedule of people in the lab.</li>
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| − | <li>Ways to integrate other students.</li>
| |
| − | <li>Events to talk about iGEM.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
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| − |
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| − | <div id="date-7-27">
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| − | <h1>Sunday, July 27th.</h1>
| |
| − | </div>
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| − |
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| − | <div id="date-7-28">
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| − | <h1>Monday, July 28th.</h1>
| |
| − | </div>
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| − |
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| − | <div id="date-7-29">
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| − | <h1>Tuesday, July 29th.</h1>
| |
| − | </div>
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| − |
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| − | <div id="date-7-30">
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| − | <h1>Wednesday, July 30th.</h1>
| |
| − | </div>
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| − |
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| − | <div id="date-7-31">
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| − | <h1>Thursday, July 31st.</h1>
| |
| − | </div>
| |
| − |
| |
| − | <!-- Agosto -->
| |
| − | <div id="date-8-1">
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| − | <h1>Friday, August 1st.</h1>
| |
| − | </div>
| |
| − |
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| − | <div id="date-8-2">
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| − | <h1>Saturday, August 2nd.</h1>
| |
| − | </div>
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| − |
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| − | <div id="date-8-3">
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| − | <h1>Sunday, August 3rd.</h1>
| |
| − | </div>
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| − |
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| − | <div id="date-8-4">
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| − | <h1>Monday, August 4th.</h1>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-5">
| |
| − | <h1>Tuesday, August 5th.</h1>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-6">
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| − | <h1>Wednesday, August 6th.</h1>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-7">
| |
| − | <h1>Thursday, August 7th.</h1>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-8">
| |
| − | <h1>Friday, August 8</h1>
| |
| − | <ul>
| |
| − | <li>Talk with new students about iGEM, 15:30.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Minerva, Mercedes, Cesar, Omar, Claudia, Eduardo Ramirez (1).</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Session with advisors, 17:00.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Mercedes, Cesar, Omar, Claudia, Eduardo Ramirez (1).</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Production of LB medium, 18:30.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Cesar.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Cleaning of glassware, 18:45.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Eduardo Ramirez (1).</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Autoclave, 19:00.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Mercedes, Cesar, Eduardo Ramirez (1).</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Grow H5-α culture, 21:00.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Cesar</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | </ul>
| |
| − | <p>Comments: Most of the progress from today’s activities was not directly related to the laboratory.
| |
| − | Keep in mind the session with the advisors next Friday (about the team rooster primarily) and the various events this
| |
| − | month and the next with the general student population.</p>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-9">
| |
| − | <h1>Saturday, August 9</h1>
| |
| − | <ul>
| |
| − | <li>Cleaning of glassware, 10:00.</li>
| |
| − | <li>Stock of the growth H5-α, 11:00.</li>
| |
| − | <li>Mini prep GFP, 15:00.</li>
| |
| − | <li>Calcium-competent of H5-α, 16:30.</li>
| |
| − | <li>Transformation of GFP (18C), GFP (18E), GFP (mini prep), RFP (O12), RFP (mini prep), (pLUX GFP). 9:40</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Omar</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Stock of the growth H5-α, 10:00.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Mercedes, Omar.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>End of transformation (plaques), 23:23.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Omar, Mercedes.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-10">
| |
| − | <h1>Sunday, August 10</h1>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-11">
| |
| − | <h1>Monday, August 11</h1>
| |
| − | <ul>
| |
| − | <li>Selection of plate’s colonies to pass to Falcon tubes of 50 ml, 7:30</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Miniprep, 14:00</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Omar</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Digestions, 19:00.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Cesar</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Electrophoresis, 21:00.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Cesar.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-12">
| |
| − | <h1>Tuesday, August 12</h1>
| |
| − | <ul>
| |
| − | <li>Making of electro-competent H5-α, 11:00.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-13">
| |
| − | <h1>Wednesday, August 13</h1>
| |
| − | <ul>
| |
| − | <li>Mini-prep of GFP, 12:00.</li>
| |
| − | <ul>
| |
| − | <li><strong>Before incubation: Minerva.</strong></li>
| |
| − | <li>After incubation: Omar, Claudia.</li>
| |
| − | </ul>
| |
| − | <li>Transformation of the electro-competent cells with RFP, 17:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Ramirez (1), Claudia, Omar.</li>
| |
| − | </ul>
| |
| − | <li>Plating of the transformed cells, 21:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Ramirez (1).</li>
| |
| − | </ul>
| |
| − | <li>Electrophoresis of GFP mini-preps and digestions of GFP (Kn) and GFP (CAM), 21:10.</li>
| |
| − | <ul>
| |
| − | <li>Cesar, Eduardo Cepeda.</li>
| |
| − | <ul>
| |
| − | <li>Failed.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-14">
| |
| − | <h1>Thursady, August 14</h1>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-15">
| |
| − | <h1>Friday, August 15</h1>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-16">
| |
| − | <h1>Saturday, August 16</h1>
| |
| − | <ul>
| |
| − | <li>Miniprep GFP, 12:00.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Digestions, 15:30.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-17">
| |
| − | <h1>Sunday, August 17</h1>
| |
| − | <ul>
| |
| − | <li>Preparation of TAE buffer, 18:00.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Digestions, 20:00.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Electrophoresis, 22:00.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Mercedes, Cesar, Claudia, Eduardo Ramirez (2), Omar.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-18">
| |
| − | <h1>Monday, August 18th.</h1>
| |
| − | <ul>
| |
| − | <li><strong>Electrophoresis, 14:00.</strong></li>
| |
| − | <ul>
| |
| − | <li>Minerva.</li>
| |
| − | </ul>
| |
| − | <li>Teaching of the newbies (Miniprep basics), 16:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Autoclave of general materials, 18:00.</li>
| |
| − | <ul>
| |
| − | <li>Everyone.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-19">
| |
| − | <h1>Tuesday, August 19</h1>
| |
| − | <ul>
| |
| − | <li>MiniPrep GFP, 12:30</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Mercedes</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Digestions, 18:00</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Electrophoresis, 21:00</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Newbies (Alex, Dany).</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-20">
| |
| − | <h1>Wednesday, August 20</h1>
| |
| − | <ul>
| |
| − | <li>iGEM talk with interested students, 16:30.</li>
| |
| − |
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Special Guest: Ana Sifuentes</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Teaching of how to make media to newbies, 18:00.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Claudia.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-21">
| |
| − | <h1>Thursday, August 21</h1>
| |
| − | <ul>
| |
| − | <li><strong>Teaching of how to make an electrophoresis gel to the newbies, 18:00.</strong></li>
| |
| − | <ul>
| |
| − | <li>Claudia.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-22">
| |
| − | <h1>Friday, August 22</h1>
| |
| − | <ul>
| |
| − | <li>Teaching of how to make a transformation in calcium-competent cells, 17:00.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Claudia.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Purification of the Minipreps of GFP and the promoters D2 and D3, 18:00.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Eduardo Ramirez (2), Omar.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Electrophoresis of the past purifications, 22:00.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Purification of the electrophoresis and ligations, 23:30.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Mercedes, Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-23">
| |
| − | <h1>Saturday, August 23</h1>
| |
| − | <ul>
| |
| − | <li><strong>Making of electro-competent cells, 9:00.</strong></li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Visit to UANL to meet their iGEM team and to hear the iGEM HQ talk, 13:30.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes, Claudia, Eduardo Ramirez (1), Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Transformations of the electro-competent cells with D2, D3 and GFP, 18:00.</li>
| |
| − | <ul>
| |
| − | <li>Claudia.</li>
| |
| − | </ul>
| |
| − | <li>Inoculation of the ligation D2-GFP, 20:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-24">
| |
| − | <h1>Sunday, August 24</h1>
| |
| − | <ul>
| |
| − | <li><strong>Miniprep of the ligation D2-GFP, 11:00.</strong></li>
| |
| − | <ul>
| |
| − | <li>Minerva.</li>
| |
| − | </ul>
| |
| − | <li>Digestion of D2, D3, GFP and RFP, 16:00.</li>
| |
| − | <ul>
| |
| − | <li>Minerva, Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Electrophoresis, 19:00.</li>
| |
| − | <ul>
| |
| − | <li>Minerva, Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>DNA purification of the gel, 20:15.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Ligations D2-GFP, D3-GFP, 21:00.</li>
| |
| − | <ul>
| |
| − | <li><strong>Mercedes.</strong></li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-25">
| |
| − | <h1>Monday, August 25</h1>
| |
| − | <ul>
| |
| − | <li><strong>Miniprep D2-GFP, 13:00.</strong></li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Digestion of D2, 16:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Inoculation practice with the newbies, 19:00.</li>
| |
| − | <ul>
| |
| − | <li>Claudia.</li>
| |
| − | </ul>
| |
| − | <li>Transformations with electro-competent cells, 21:30.</li>
| |
| − | <ul>
| |
| − | <li>Cesar.</li>
| |
| − | </ul>
| |
| − | <li>Preparation of plaques, 23:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-26">
| |
| − | <h1>Tuesday, August 26</h1>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-27">
| |
| − | <h1>Wednesday, August 27</h1>
| |
| − | <ul>
| |
| − | <li>Inoculm of BL21 7:00.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Miniprep D2-GFP, D3-GFP, D2-RFP, D3-RFP, 13:30.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Digestions, 18:00.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Electrophoresis, 20:00</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Preparation of calcium-competents BL21,</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Claudia, Eduardo Ramirez.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-28">
| |
| − | <h1>Thursday, August 28</h1>
| |
| − | <ul>
| |
| − | <li>Electrophoresis, 22:00.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Newbies.</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | <li>Preparation of calcium-competent cells TOP10, 22:00.</li>
| |
| − | <li style="list-style: none; display: inline">
| |
| − | <ul>
| |
| − | <li>Eduardo Ramirez (2).</li>
| |
| − | </ul>
| |
| − | </li>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-29">
| |
| − | <h1>Friday, August 29</h1>
| |
| − | <ul>
| |
| − | <li><strong>Preparation of chloramphenicol antibiotic, 10:00.</strong></li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Miniprep of D2-GFP and D3-GFP, 13:00.</li>
| |
| − | <ul>
| |
| − | <li>Omar, Minerva.</li>
| |
| − | </ul>
| |
| − | <li>Miniprep of D2 and D3 promoters, 15:00.</li>
| |
| − | <ul>
| |
| − | <li>Omar.</li>
| |
| − | </ul>
| |
| − | <li>Purification of the minipreps, 20:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>?</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-30">
| |
| − | <h1>Saturday, August 30</h1>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-8-31">
| |
| − | <h1>Sunday, August 31</h1>
| |
| − | <ul>
| |
| − | <li><strong>Miniprep GFP Kn, RFP, 3 replicas of GFP Cam, 15:00.</strong></li>
| |
| − | <ul>
| |
| − | <li>Claudia, Eduardo Ramirez (2).</li>
| |
| − | </ul>
| |
| − | <li>Digestions, 18:30.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Electrophoresis, 22:40.</li>
| |
| − | <ul>
| |
| − | <li>Omar.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <!-- September -->
| |
| − | <div id="date-9-1">
| |
| − | <h1>Monday, September 1st.</h1>
| |
| − | <ul>
| |
| − | <li>Apoptosis kit arrived!, 14:00.</li>
| |
| − | <li>Accutase arrived!, 14:00.</li>
| |
| − | <li>Genes arrived!, 14:00.</li>
| |
| − | <li>Preparation of LB and LB-agar media, 13:30.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Ramirez (1).</li>
| |
| − | </ul>
| |
| − | <li>Preparation of the plaques for the transformation, 17:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | <table>
| |
| − | <tbody>
| |
| − | <tr>
| |
| − | <td>
| |
| − | <p>Abbreviation</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>Construct</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>Gene</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>Resistance</p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr>
| |
| − | <td>
| |
| − | <p>LPP</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>C1</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>LPP Red Lambda</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>Amp</p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr>
| |
| − | <td>
| |
| − | <p>MSBB</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>C2</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>Red Lambda</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>Kn + Amp</p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr>
| |
| − | <td>
| |
| − | <p>H-GFP</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>C4</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>GFP</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>Amp</p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr>
| |
| − | <td>
| |
| − | <p>C-Apop</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>C5</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>Apoptine</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>Amp</p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr>
| |
| − | <td>
| |
| − | <p>H-SiRNA 1</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>C6</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>SiRNA 1</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>Amp</p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr>
| |
| − | <td>
| |
| − | <p>H-SiRNA 2</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>C7</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>SiRNA 2</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>Amp</p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr>
| |
| − | <td>
| |
| − | <p>H-SiRNA 3</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>C8</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>SiRNA 3</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>Amp</p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr>
| |
| − | <td>
| |
| − | <p>H-SiRNA-Apop</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>C9</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>SiRNA + Apoptine</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>Amp</p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr>
| |
| − | <td>
| |
| − | <p>BBa_1</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>Biobrick</p>
| |
| − | </td>
| |
| − | <td> </td>
| |
| − | <td>
| |
| − | <p>Cam</p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr>
| |
| − | <td>
| |
| − | <p>BBa_2</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>Biobrick</p>
| |
| − | </td>
| |
| − | <td> </td>
| |
| − | <td>
| |
| − | <p>Cam</p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | <tr>
| |
| − | <td>
| |
| − | <p>BBa_3</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>Biobrick</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>Peptide + p3</p>
| |
| − | </td>
| |
| − | <td>
| |
| − | <p>Cam</p>
| |
| − | </td>
| |
| − | </tr>
| |
| − | </tbody>
| |
| − | </table>
| |
| − | <ul>
| |
| − | <li>HyperLadder 50bp arrived!, 17:10</li>
| |
| − | <li>HLA arrived!, 17:10:</li>
| |
| − | <li>Transformation of C1 through C7 in TOP10, 19:00.</li>
| |
| − | <ul>
| |
| − | <li>Minerva.</li>
| |
| − | </ul>
| |
| − | <li>Transformation of GFP and RFP in TOP10, 21:30.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-2">
| |
| − | <h1>Tuesday, September 2</h1>
| |
| − | <ul>
| |
| − | <li>Inoculation of plates with transformed colonies from yesterday, 12:00.</li>
| |
| − | <ul>
| |
| − | <li>Minerva.</li>
| |
| − | </ul>
| |
| − | <li>Transformations of GFP Amp, RFP Cam, D1 Kn, D2 Cam, D3 Amp, 19:30.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Ramirez (2).</li>
| |
| − | </ul>
| |
| − | <li>Miniprep of the grown transformed cells from yesterday (C4, C5, C6, C7, D2, GFP, D1, BBa_1), 21:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-3">
| |
| − | <h1>Wednesday, September 3</h1>
| |
| − | <ul>
| |
| − | <li>Digestions of the last minipreps, 02:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Electrophoresis, 03:30.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Preparation of electro-competent TOP10 cells, 08:00.</li>
| |
| − | <ul>
| |
| − | <li>Newbies: Alex, Danny, Chuy, Caro.</li>
| |
| − | </ul>
| |
| − | <li>Preparation of stock cells (C4, C5, C6, C7, D2, GFP, D1, BBa_1), 18:30.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Ligations of the C4, C5, C6, C7 with the pcb1c3, 20:30.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Inoculation of TOP10 for electro-competent cells tomorrow and D2 cells, 01:25.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-4">
| |
| − | <h1>Thursday, September 4</h1>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-5">
| |
| − | <h1>Friday, September 5</h1>
| |
| − | <ul>
| |
| − | <li>Preparation of the tickets for the cake raffle (fundraising), 12:00.</li>
| |
| − | <ul>
| |
| − | <li>Cinthya, Omar, Eduardo Ramirez (1), Eduardo Cepeda, Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Miniprep, 14:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes and Eduardo Cepeda with special guest: Ana Cristina.</li>
| |
| − | </ul>
| |
| − | <li>Talk with Professor Cardineau about the legislation proposal, 17:00.</li>
| |
| − | <ul>
| |
| − | <li>Omar.</li>
| |
| − | </ul>
| |
| − | <li>Preparations of electro-competente TOP10 cells, 17:30.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Ramirez (2), Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Digestions, 19:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Inoculation of LB-agar plaques, 21:00.</li>
| |
| − | <ul>
| |
| − | <li>Cynthia.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-6">
| |
| − | <h1>Saturday, September 6</h1>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-7">
| |
| − | <h1>Sunday, September 7</h1>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-8">
| |
| − | <h1>Monday, September 8</h1>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-9">
| |
| − | <h1>Tuesday, September 9</h1>
| |
| − | <ul>
| |
| − | <li>Inoculation of PCB1c3 (with C4, C5, C7 separately), 13:30.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-10">
| |
| − | <h1>Wednesday, September 10</h1>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-11">
| |
| − | <h1>Thursday, September 11</h1>
| |
| − | <ul>
| |
| − | <li>Revision of (not grown yet) plates, 12:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Inoculaton of D2-GFP, 21:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-12">
| |
| − | <h1>Friday, September 12</strong><strong>th</strong></h1>
| |
| − | <ul>
| |
| − | <li>Cleaning of the electroporation cells for the electro-competent cells, 16:00.</li>
| |
| − | <ul>
| |
| − | <li>Minerva.</li>
| |
| − | </ul>
| |
| − | <li>Inoculation of cells that grew from the prior day, 18:00.</li>
| |
| − | <ul>
| |
| − | <li>Cinthya.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-13">
| |
| − | <h1>Saturday, September 13</h1>
| |
| − | <ul>
| |
| − | <li>Transformation of the D2 and D3 variants for the interlab project, 00:10.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Ramirez (2), Mercedes, Claudia, Omar.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-14">
| |
| − | <h1>Sunday, September 14th.</strong></h1>
| |
| − | <ul>
| |
| − | <li>Miniprep, 17:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes, Eduardo Ramirez (2)</li>
| |
| − | </ul>
| |
| − | <li>Sterilization of Falcon tubes, LB broth and plastic pearls, 18:30.</li>
| |
| − | <ul>
| |
| − | <li>Omar</li>
| |
| − | </ul>
| |
| − | <li>Digestions, 21:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Inoculation of the transformed cells with D2 and D3 variants, 22:00.</li>
| |
| − | <ul>
| |
| − | <li>Omar.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-15">
| |
| − | <h1>Monday, September 15</h1>
| |
| − | <ul>
| |
| − | <li>Miniprep of yesterday's inoculations of D2 and D3 variants, 10:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Digestions, 17:30.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Ramirez (2).</li>
| |
| − | </ul>
| |
| − | <li>Inoculation of TOP10 and XL-Blue (Tet), 22:50.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-16">
| |
| − | <h1>Tuesday, September 16th.</h1>
| |
| − | <ul>
| |
| − | <li>Transformations of the M13 bacteriophage into the XL-Blue, 10:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-17">
| |
| − | <h1>Wednesday, September 17</h1>
| |
| − | <ul>
| |
| − | <li>Miniprep 01:15.</li>
| |
| − | <ul>
| |
| − | <li>Minerva, Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Digestions (C4, C5, C6, C7 and , 02:53.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>PBS buffer, 12:30.</li>
| |
| − | <ul>
| |
| − | <li>Omar.</li>
| |
| − | </ul>
| |
| − | <li>Recovery of DNA from Gel, 13:30.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-18">
| |
| − | <h1>Thursday, September 18</h1>
| |
| − | <ul>
| |
| − | <li>Transformations of C8, C9 and ligation in TOP10 electro-competent, 00:40.</li>
| |
| − | <ul>
| |
| − | <li>Cinthya, Omar.</li>
| |
| − | </ul>
| |
| − | <li>Electrophoresis, 01:30.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes, Eduardo Ramirez (1).</li>
| |
| − | </ul>
| |
| − | <li>Plates from the transformations, 01:50.</li>
| |
| − | <ul>
| |
| − | <li>Omar.</li>
| |
| − | </ul>
| |
| − | <li>Miniprep of PET, 12:30.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Transformation replica of the same ligation as before, 15:30.</li>
| |
| − | <ul>
| |
| − | <li>Omar.</li>
| |
| − | </ul>
| |
| − | <li>Preparation of LB broth, 17:00.</li>
| |
| − | <ul>
| |
| − | <li>All the newbies (Caro, Jesus, Alex, Dany).</li>
| |
| − | </ul>
| |
| − | <li>Digestion of PET, 19:30.</li>
| |
| − | <ul>
| |
| − | <li>Claudia.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-19">
| |
| − | <h1>Friday, September 19</h1>
| |
| − | <ul>
| |
| − | <li>Ligation of PET with all the constructs, 01:19.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Electrophoresis, 03:30.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Miniprep, 8:30.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Digestions, 13:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Electrophoresis, 15:30.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Quantification of the fluorescence for the interlab project, 16:30.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes, Eduardo Ramirez (2).</li>
| |
| − | </ul>
| |
| − | <li>Group photo taken, 18:00.</li>
| |
| − | <ul>
| |
| − | <li>Everyone except Mercedes, Eduardo Cepeda and Eduardo Ramirez.</li>
| |
| − | </ul>
| |
| − | <li>Group video filmed, 18:30.</li>
| |
| − | <ul>
| |
| − | <li>Everyone.</li>
| |
| − | </ul>
| |
| − | <li>Transformation of TOP10 cells with PET + (C4, C5, C6, C7), 20:30.</li>
| |
| − | <ul>
| |
| − | <li>Cinthya, Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Transformation of TOP10 cells with various repetitions of D2 and D3 (with GFP or RFP), 21:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes, Eduardo Ramirez (2).</li>
| |
| − | </ul>
| |
| − | <li>Inoculation in Falcon tubes with the ligation of C5 and various others, 22:00.</li>
| |
| − | <ul>
| |
| − | <li>Omar.</li>
| |
| − | </ul>
| |
| − | <li>Inoculation in plates of the previous transformed TOP10 cells with PET, 23:00.</li>
| |
| − | <ul>
| |
| − | <li>Cinthya.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-20">
| |
| − | <h1>Saturday, September 20</h1>
| |
| − | <ul>
| |
| − | <li>Transformation in electro-competent cells, 01:20.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes, Eduardo Ramirez.</li>
| |
| − | </ul>
| |
| − | <li>Minipreps, 18:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes, Omar.</li>
| |
| − | </ul>
| |
| − | <li>Digestions, 22:00.</li>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-21">
| |
| − | <h1>Sunday, September 21</strong><strong>st</strong><strong>.</strong></h1>
| |
| − | <ul>
| |
| − | <li>Electrophoresis, 00:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Inoculations for minipreps, 01:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Minipreps,</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-22">
| |
| − | <h1>Monday, September 22</h1>
| |
| − | <ul>
| |
| − | <li>Miniprep, 08:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Revision of the cell stocks, contamination detected, 09:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Inoculations of C8 and C9, 22:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-23">
| |
| − | <h1>Tuesday, September 23</h1>
| |
| − | <ul>
| |
| − | <li>Preparation of electo-competent cells DH5a, 03:00.</li>
| |
| − | <ul>
| |
| − | <li>Minerva.</li>
| |
| − | </ul>
| |
| − | <li>Minipreps for the interlab, 06:00</li>
| |
| − | <ul>
| |
| − | <li>Mercedes, Minerva.</li>
| |
| − | </ul>
| |
| − | <li>Transformations for the interlab, 07:30.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes, Minerva.</li>
| |
| − | </ul>
| |
| − | <li>Digestions for the interlab, 12:30.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Miniprep, 13:30.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Electrophoresis for interlab, 19:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Ramirez (2)</li>
| |
| − | </ul>
| |
| − | <li>Electrophoresis for the project (C8, C9), 21:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Ramirez (2)</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-24">
| |
| − | <h1>Wednesday, September 24</h1>
| |
| − | <ul>
| |
| − | <li>Purification of the electrophoresis gel, 01:20.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Digestions for the interlab, 01:20.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Ligations, 02:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Electrophoresis for the interlab, 02:40.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Inoculations for the interlab, 11:00.</li>
| |
| − | <ul>
| |
| − | <li>Omar, Mercedes.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-25">
| |
| − | <h1>Thursday, September 25</h1>
| |
| − | <ul>
| |
| − | <li>Inoculation of XL-Blue, 22:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Infection of the XL-Blue with bacteriophages, 23:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-26">
| |
| − | <h1>Friday, September 26</h1>
| |
| − | <ul>
| |
| − | <li>Miniprep of the bacteriophage-infected cells, 03:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Preparation of the cells for quantification of fluorescence and OD for the interlab project, from 8:00 to 24:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Purification of the miniprep. 18:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Ramirez (1), Claudia.</li>
| |
| − | </ul>
| |
| − | <li>Transformations of C1, C2, Bba_1, Bba_2, and ligations, 19:00.</li>
| |
| − | <ul>
| |
| − | <li>Cinthya, Omar.</li>
| |
| − | </ul>
| |
| − | <li>Plating of the transformations, 22:00.</li>
| |
| − | <ul>
| |
| − | <li>Cinthya.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-27">
| |
| − | <h1>Saturday, September 27</h1>
| |
| − | <ul>
| |
| − | <li>Inoculation of C1, C2, Bba_1, Bba _2 and ligations, 20:00.</li>
| |
| − | <ul>
| |
| − | <li>Omar</li>
| |
| − | </ul>
| |
| − | <li>Sterilization of material, 22:00.</li>
| |
| − | <ul>
| |
| − | <li>Omar.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-28">
| |
| − | <h1>Sunday, September 28</h1>
| |
| − | <ul>
| |
| − | <li>Preparation of electro-competent cells, 01:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Ligations, 03:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Electrophoresis, 12:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Minipreps of the inoculations from Saturday, 13:00.</li>
| |
| − | <ul>
| |
| − | <li>Claudia, Eduardo Ramirez (1).</li>
| |
| − | </ul>
| |
| − | <li>Recovery of DNA from gel, 15:00.</li>
| |
| − | <ul>
| |
| − | <li>Claudia.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-29">
| |
| − | <h1>Monday, September 29</h1>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-9-30">
| |
| − | <h1>Tuesday, September 30</h1>
| |
| − | </div>
| |
| − |
| |
| − | <!-- October -->
| |
| − | <div id="date-10-1">
| |
| − | <h1>Wednesday, October 1st.</h1>
| |
| − | <ul>
| |
| − | <li>Minipreps of all constructs, 12:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo.</li>
| |
| − | </ul>
| |
| − | <li>Digestions of all constructs, 20:30.</li>
| |
| − | <ul>
| |
| − | <li>Claudia, Jesus.</li>
| |
| − | </ul>
| |
| − | <li>Miniprep of C2, 21:00.</li>
| |
| − | <ul>
| |
| − | <li>Jesus.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-10-2">
| |
| − | <h1>Thursday, October 2nd.</h1>
| |
| − | <ul>
| |
| − | <li>Collaboration: gave C4 through C9 to the UANL team, 13:30.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Inoculo XL-Blue, 18:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Ramirez (2).</li>
| |
| − | </ul>
| |
| − | <li>Miniprep C8, C10, Bb_1, C6, 16:30.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda, Eduardo Ramirez (2).</li>
| |
| − | </ul>
| |
| − | <li>Miniprep of phagemides, 18:30.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-10-3">
| |
| − | <h1>Friday, October 3rd.</h1>
| |
| − | <ul>
| |
| − | <li>Digestions of C1, C2 and PET variants of the other constructs, 12:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Minipreps, 12:00.</li>
| |
| − | <ul>
| |
| − | <li>Claudia.</li>
| |
| − | </ul>
| |
| − | <li>Electrophoresis, 16:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Inoculations, 20:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-10-4">
| |
| − | <h1>Saturday, October 4th.</h1>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-10-5">
| |
| − | <h1>Sunday, October 5th.</h1>
| |
| − | <ul>
| |
| − | <li>Western Blot, 01:00.</li>
| |
| − | <ul>
| |
| − | <li>Minerva.</li>
| |
| − | </ul>
| |
| − | <li>Minipreps, 00:30.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Electrophoresis, 04:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Inoculations, 18:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-10-6">
| |
| − | <h1>Monday, October 6th.</h1>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-10-7">
| |
| − | <h1>Tuesday, October 7th.</h1>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-10-8">
| |
| − | <h1>Wednesday, October 8th.</h1>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-10-9">
| |
| − | <h1>Thursday, October 9th.</h1>
| |
| − | <ul>
| |
| − | <li>Minipreps for cells with flippase, 12:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Stock of cells with flippase, 12:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Digestions for flippase, 16:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Electrophoresis, 18:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-10-10">
| |
| − | <h1>Friday, October 10th.</h1>
| |
| − | <ul>
| |
| − | <li>Digestions of Bba_1, Bba_2 and Bba_3, 08:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Digestion of the modified phage, 09:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Electrophoresis, 12:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Ramirez (2).</li>
| |
| − | </ul>
| |
| − | <li>Ligation of the phages back to circle form, 13:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-10-11">
| |
| − | <h1>Saturday, October 11th.</h1>
| |
| − | <ul>
| |
| − | <li>Inoculations for constructs stocks, 20:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-10-12">
| |
| − | <h1>Sunday, October 12th.</h1>
| |
| − | <ul>
| |
| − | <li>Pictures taken for the wiki, 13:00.</li>
| |
| − | <ul>
| |
| − | <li>Everyone.</li>
| |
| − | </ul>
| |
| − | <li>PCR, 16:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Stocks, 18:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Electrophoresis, 20:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-10-13">
| |
| − | <h1>Monday, October 13th.</h1>
| |
| − | <ul>
| |
| − | <li>PCR for Red Lambda confirmation, 13:00.</li>
| |
| − | <ul>
| |
| − | <li>Eduardo Cepeda.</li>
| |
| − | </ul>
| |
| − | <li>Electrophoresis, 15:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>Digestions, 16:00.</li>
| |
| − | <ul>
| |
| − | <li>Mercedes.</li>
| |
| − | </ul>
| |
| − | <li>TAE buffer preparation, 20:00.</li>
| |
| − | <ul>
| |
| − | <li>Newbies: Alejandro, Danny.</li>
| |
| − | </ul>
| |
| − | <li>Western Blott, 21:00.</li>
| |
| − | <ul>
| |
| − | <li>Minerva, Claudia.</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-10-14">
| |
| − | <h1>Tuesday, October 14th.</h1>
| |
| − | <ul>
| |
| − | <li>Work on wiki</li>
| |
| − | <ul>
| |
| − | <li>Sebastián Valdivieso, Eduardo Zardain, Eduardo Ramírez</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-10-15">
| |
| − | <h1>Wednesday, October 15th.</h1>
| |
| − | <ul>
| |
| − | <li>Work on wiki</li>
| |
| − | <ul>
| |
| − | <li>Sebastián Valdivieso, Eduardo Zardain, Eduardo Ramírez</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-10-16">
| |
| − | <h1>Thursday, October 16th.</h1>
| |
| − | <ul>
| |
| − | <li>Work on wiki</li>
| |
| − | <ul>
| |
| − | <li>Sebastián Valdivieso, Eduardo Zardain, Eduardo Ramírez</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div id="date-10-17">
| |
| − | <h1>Friday, October 17th.</h1>
| |
| − | <ul>
| |
| − | <li>Work on wiki, its wiki-freeze day!!!</li>
| |
| − | <ul>
| |
| − | <li>Sebastián Valdivieso, Eduardo Zardain, Eduardo Ramírez</li>
| |
| − | </ul>
| |
| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | </body>
| |
| − | </html>
| |
