Difference between revisions of "Team:Manchester/Notebook/data.html"

(Created page with "<html> <head> <title></title> </head> <body> <!-- Junio --> <div id="date-6-1"> <h1>Sunday, June 1st.</h1> </div> <div id="date-6-2"> <h1>Monday, June 2nd.</h1> <u...")
 
Line 5: Line 5:
  
 
<body>
 
<body>
−
<!-- Junio -->
+
<!-- June -->
 
<div id="date-6-1">
 
<div id="date-6-1">
−
   <h1>Sunday, June 1st.</h1>
+
   <h1>Thursday, June 1st.</h1>
 
</div>
 
</div>
  
 
<div id="date-6-2">   
 
<div id="date-6-2">   
−
   <h1>Monday, June 2nd.</h1>
+
   <h1>Friday, June 2nd.</h1>
 
   <ul>
 
   <ul>
 
     <li>Lab cleanup, 12:00.</li>
 
     <li>Lab cleanup, 12:00.</li>
Line 21: Line 21:
  
 
<div id="date-6-3">
 
<div id="date-6-3">
−
   <h1>Tuesday, June 3rd.</h1>
+
   <h1>Saturday, June 3rd.</h1>
 
</div>
 
</div>
  
 
<div id="date-6-4">
 
<div id="date-6-4">
−
   <h1>Wednesday, June 5th.</h1>
+
   <h1>Sunday, June 4th.</h1>
 
</div>
 
</div>
  
 
<div id="date-6-5">
 
<div id="date-6-5">
−
   <h1>Thursday, June 5th.</h1>
+
   <h1>Monday, June 5th.</h1>
 
</div>
 
</div>
  
 
<div id="date-6-6">
 
<div id="date-6-6">
−
   <h1>Friday, June 6th.</h1>
+
   <h1>Tuesday, June 6th.</h1>
 
   <ul>
 
   <ul>
 
     <li>iGEM 2014 meeting to talk about lab work, 14:00.</li>
 
     <li>iGEM 2014 meeting to talk about lab work, 14:00.</li>
Line 43: Line 43:
  
 
<div id="date-6-7">
 
<div id="date-6-7">
−
   <h1>Saturday, June 7th.</h1>
+
   <h1>Wednesday, June 7th.</h1>
 
</div>
 
</div>
  
 
<div id="date-6-8">
 
<div id="date-6-8">
−
   <h1>Sunday, June 8th.</h1>
+
   <h1>Thursday, June 8th.</h1>
 
</div>
 
</div>
  
 
<div id="date-6-9">
 
<div id="date-6-9">
−
   <h1>Monday, June 9th.</h1>
+
   <h1>Friday, June 9th.</h1>
 
</div>
 
</div>
  
 
<div id="date-6-10">
 
<div id="date-6-10">
−
   <h1>Tuesday, June 10th.</h1>
+
   <h1>Saturday, June 10th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Revision of the protocols for next month&rsquo;s lab-work, 12:00.</li>
 
     <li>Revision of the protocols for next month&rsquo;s lab-work, 12:00.</li>
Line 69: Line 69:
  
 
<div id="date-6-11">
 
<div id="date-6-11">
−
   <h1>Wednesday, June 11th.</h1>
+
   <h1>Sunday, June 11th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Electro-competent cells, 13:00.</li>
 
     <li>Electro-competent cells, 13:00.</li>
Line 79: Line 79:
  
 
<div id="date-6-12">
 
<div id="date-6-12">
−
   <h1>Thursday, June 12th.</h1>
+
   <h1>Monday, June 12th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Sponsorships, all day.</li>
 
     <li>Sponsorships, all day.</li>
Line 89: Line 89:
  
 
<div id="date-6-13">
 
<div id="date-6-13">
−
   <h1>Friday, June 13th.</h1>
+
   <h1>Tuesday, June 13th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Meeting with advisors, 17:00.</li>
 
     <li>Meeting with advisors, 17:00.</li>
Line 100: Line 100:
  
 
<div id="date-6-14">
 
<div id="date-6-14">
−
   <h1>Saturday, June 14th.</h1>
+
   <h1>Wednesday, June 14th.</h1>
 
</div>
 
</div>
  
 
<div id="date-6-15">
 
<div id="date-6-15">
−
   <h1>Sunday, June 15th.</h1>
+
   <h1>Thursday, June 15th.</h1>
 
</div>
 
</div>
  
 
<div id="date-6-16">
 
<div id="date-6-16">
−
   <h1>Monday, June 16th.</h1>
+
   <h1>Friday, June 16th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Electro-competent cells, 14:00.</li>
 
     <li>Electro-competent cells, 14:00.</li>
Line 130: Line 130:
  
 
<div id="date-6-17">
 
<div id="date-6-17">
−
   <h1>Tuesday, June 17th.</h1>
+
   <h1>Saturday, June 17th.</h1>
 
</div>
 
</div>
  
 
<div id="date-6-18">
 
<div id="date-6-18">
−
   <h1>Wednesday, June 18th.</h1>
+
   <h1>Sunday, June 18th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Electro-competent cells, 10:00.</li>
 
     <li>Electro-competent cells, 10:00.</li>
Line 156: Line 156:
  
 
<div id="date-6-19">
 
<div id="date-6-19">
−
   <h1>Thursday, June 19th.</h1>
+
   <h1>Monday, June 19th.</h1>
 
</div>
 
</div>
  
 
<div id="date-6-20">
 
<div id="date-6-20">
−
   <h1>Friday, June 20th.</h1>
+
   <h1>Tuesday, June 20th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Meeting with advisors, 17:00.</li>
 
     <li>Meeting with advisors, 17:00.</li>
Line 167: Line 167:
  
 
<div id="date-6-21">
 
<div id="date-6-21">
−
   <h1>Saturday, June 21st.</h1>
+
   <h1>Wednesday, June 21st.</h1>
 
</div>
 
</div>
  
 
<div id="date-6-22">
 
<div id="date-6-22">
−
   <h1>Sunday, June 22nd.</h1>
+
   <h1>Thursday, June 22nd.</h1>
 
</div>
 
</div>
  
 
<div id="date-6-23">
 
<div id="date-6-23">
−
   <h1>Monday, June 23rd.</h1>
+
   <h1>Friday, June 23rd.</h1>
 
   <ul>
 
   <ul>
 
     <li>Calcium-competent cells, 12:00.</li>
 
     <li>Calcium-competent cells, 12:00.</li>
Line 185: Line 185:
  
 
<div id="date-6-24">
 
<div id="date-6-24">
−
   <h1>Tuesday, June 24th.</h1>
+
   <h1>Saturday, June 24th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Minipreps, 11:00</li>
 
     <li>Minipreps, 11:00</li>
Line 203: Line 203:
  
 
<div id="date-6-25">
 
<div id="date-6-25">
−
   <h1>Wednesday, June 25nd.</h1>
+
   <h1>Sunday, June 25th.</h1>
 
</div>
 
</div>
  
 
<div id="date-6-26">
 
<div id="date-6-26">
−
   <h1>Thursday, June 26th.</h1>
+
   <h1>Monday, June 26th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Minipreps, 10:00</li>
 
     <li>Minipreps, 10:00</li>
Line 221: Line 221:
  
 
<div id="date-6-27">
 
<div id="date-6-27">
−
   <h1>Friday, June 27th.</h1>
+
   <h1>Tuesday, June 27th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Meeting with advisors, 17:00.</li>
 
     <li>Meeting with advisors, 17:00.</li>
Line 228: Line 228:
  
 
<div id="date-6-28">
 
<div id="date-6-28">
−
   <h1>Saturday, June 28nd.</h1>
+
   <h1>Wednesday, June 28th.</h1>
 
</div>
 
</div>
  
 
<div id="date-6-29">
 
<div id="date-6-29">
−
   <h1>Sunday, June 29nd.</h1>
+
   <h1>Thursday, June 29th.</h1>
 
</div>
 
</div>
  
 
<div id="date-6-30">
 
<div id="date-6-30">
−
   <h1>Monday, June 30nd.</h1>
+
   <h1>Friday, June 30th.</h1>
 
</div>
 
</div>
  
  
−
<!-- Julio -->
+
<!-- July -->
 
<div id="date-7-1">
 
<div id="date-7-1">
−
   <h1>Tuesday, July 1st.</h1>
+
   <h1>Saturday, July 1st.</h1>
 
   <ul>
 
   <ul>
 
     <li>Practice: Minipreps, 13:00.</li>
 
     <li>Practice: Minipreps, 13:00.</li>
Line 256: Line 256:
  
 
<div id="date-7-2">
 
<div id="date-7-2">
−
   <h1>Wednesday, July 2nd.</h1>
+
   <h1>Sunday, July 2nd.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-3">
 
<div id="date-7-3">
−
   <h1>Thursday, July 3rd.</h1>
+
   <h1>Monday, July 3rd.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-4">
 
<div id="date-7-4">
−
   <h1>Friday, July 4th.</h1>
+
   <h1>Tuesday, July 4th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Minipreps, 12:00.</li>
 
     <li>Minipreps, 12:00.</li>
Line 282: Line 282:
  
 
<div id="date-7-5">
 
<div id="date-7-5">
−
   <h1>Saturday, July 5th.</h1>
+
   <h1>Wednesday, July 5th.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-6">
 
<div id="date-7-6">
−
   <h1>Sunday, July 6th.</h1>
+
   <h1>Thursday, July 6th.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-7">
 
<div id="date-7-7">
−
   <h1>Monday, July 7th.</h1>
+
   <h1>Friday, July 7th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Sendoffs of our teammates for a deserved vacation through the next 2 weeks.</li>
 
     <li>Sendoffs of our teammates for a deserved vacation through the next 2 weeks.</li>
Line 302: Line 302:
  
 
<div id="date-7-8">
 
<div id="date-7-8">
−
   <h1>Tuesday, July 8th.</h1>
+
   <h1>Saturday, July 8th.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-9">
 
<div id="date-7-9">
−
   <h1>Wednesday, July 9th.</h1>
+
   <h1>Sunday, July 9th.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-10">
 
<div id="date-7-10">
−
   <h1>Thursday, July 10th.</h1>
+
   <h1>Monday, July 10th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Practice: Transformation of calcium-competent cells, 11:00,</li>
 
     <li>Practice: Transformation of calcium-competent cells, 11:00,</li>
Line 328: Line 328:
  
 
<div id="date-7-11">
 
<div id="date-7-11">
−
   <h1>Friday, July 11th.</h1>
+
   <h1>Tuesday, July 11th.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-12">
 
<div id="date-7-12">
−
   <h1>Saturday, July 12th.</h1>
+
   <h1>Wednesday, July 12th.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-13">
 
<div id="date-7-13">
−
   <h1>Sunday, July 13th.</h1>
+
   <h1>Thursday, July 13th.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-14">
 
<div id="date-7-14">
−
   <h1>Monday, July 14th.</h1>
+
   <h1>Friday, July 14th.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-15">
 
<div id="date-7-15">
−
   <h1>Tuesday, July 15th.</h1>
+
   <h1>Saturday, July 15th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Practice: taking DNA out of the iGEM plates, 13:00.</li>
 
     <li>Practice: taking DNA out of the iGEM plates, 13:00.</li>
Line 362: Line 362:
  
 
<div id="date-7-16">
 
<div id="date-7-16">
−
   <h1>Wednesday, July 16th.</h1>
+
   <h1>Sunday, July 16th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Minipreps, 12:00.</li>
 
     <li>Minipreps, 12:00.</li>
Line 381: Line 381:
  
 
<div id="date-7-17">
 
<div id="date-7-17">
−
   <h1>Thursday, July 17th.</h1>
+
   <h1>Monday, July 17th.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-18">
 
<div id="date-7-18">
−
   <h1>Friday, July 18th.</h1>
+
   <h1>Tuesday, July 18th.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-19">
 
<div id="date-7-19">
−
   <h1>Saturday, July 19th.</h1>
+
   <h1>Wednesday, July 19th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Meeting with advisors to talk about gene problems and doubts, 17:00.</li>
 
     <li>Meeting with advisors to talk about gene problems and doubts, 17:00.</li>
Line 396: Line 396:
  
 
<div id="date-7-20">
 
<div id="date-7-20">
−
   <h1>Sunday, July 20th.</h1>
+
   <h1>Thursday, July 20th.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-21">
 
<div id="date-7-21">
−
   <h1>Monday, July 21st.</h1>
+
   <h1>Friday, July 21st.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-22">
 
<div id="date-7-22">
−
   <h1>Tuesday, July 22nd.</h1>
+
   <h1>Saturday, July 22nd.</h1>
 
   <ul>
 
   <ul>
 
     <li>Minipreps, 11:00.</li>
 
     <li>Minipreps, 11:00.</li>
Line 426: Line 426:
  
 
<div id="date-7-23">
 
<div id="date-7-23">
−
   <h1>Wednesday, July 23rd.</h1>
+
   <h1>Sunday, July 23rd.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-24">
 
<div id="date-7-24">
−
   <h1>Thursday, July 24th.</h1>
+
   <h1>Monday, July 24th.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-25">
 
<div id="date-7-25">
−
   <h1>Friday, July 25th.</h1>
+
   <h1>Tuesday, July 25th.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-26">
 
<div id="date-7-26">
−
   <h1>Saturday, July 26th.</h1>
+
   <h1>Wednesday, July 26th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Meeting with advisors, 17:00.</li>
 
     <li>Meeting with advisors, 17:00.</li>
Line 455: Line 455:
  
 
<div id="date-7-27">
 
<div id="date-7-27">
−
   <h1>Sunday, July 27th.</h1>
+
   <h1>Thursday, July 27th.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-28">
 
<div id="date-7-28">
−
   <h1>Monday, July 28th.</h1>
+
   <h1>Friday, July 28th.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-29">
 
<div id="date-7-29">
−
   <h1>Tuesday, July 29th.</h1>
+
   <h1>Saturday, July 29th.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-30">
 
<div id="date-7-30">
−
   <h1>Wednesday, July 30th.</h1>
+
   <h1>Sunday, July 30th.</h1>
 
</div>
 
</div>
  
 
<div id="date-7-31">
 
<div id="date-7-31">
−
   <h1>Thursday, July 31st.</h1>
+
   <h1>Monday, July 31st.</h1>
 
</div>
 
</div>
  
−
<!-- Agosto -->
+
<!-- August -->
 
<div id="date-8-1">
 
<div id="date-8-1">
−
   <h1>Friday, August 1st.</h1>
+
   <h1>Tuesday, August 1st.</h1>
 
</div>
 
</div>
  
 
<div id="date-8-2">
 
<div id="date-8-2">
−
   <h1>Saturday, August 2nd.</h1>
+
   <h1>Wednesday, August 2nd.</h1>
 
</div>
 
</div>
  
 
<div id="date-8-3">
 
<div id="date-8-3">
−
   <h1>Sunday, August 3rd.</h1>
+
   <h1>Thursday, August 3rd.</h1>
 
</div>
 
</div>
  
 
<div id="date-8-4">
 
<div id="date-8-4">
−
   <h1>Monday, August 4th.</h1>
+
   <h1>Friday, August 4th.</h1>
 
</div>
 
</div>
  
 
<div id="date-8-5">
 
<div id="date-8-5">
−
   <h1>Tuesday, August 5th.</h1>
+
   <h1>Saturday, August 5th.</h1>
 
</div>
 
</div>
  
 
<div id="date-8-6">
 
<div id="date-8-6">
−
   <h1>Wednesday, August 6th.</h1>
+
   <h1>Sunday, August 6th.</h1>
 
</div>
 
</div>
  
 
<div id="date-8-7">
 
<div id="date-8-7">
−
   <h1>Thursday, August 7th.</h1>
+
   <h1>Monday, August 7th.</h1>
 
</div>
 
</div>
  
 
<div id="date-8-8">
 
<div id="date-8-8">
−
   <h1>Friday, August 8</h1>
+
   <h1>Tuesday, August 8th</h1>
 
   <ul>
 
   <ul>
 
     <li>Talk with new students about iGEM, 15:30.</li>
 
     <li>Talk with new students about iGEM, 15:30.</li>
Line 549: Line 549:
  
 
<div id="date-8-9">
 
<div id="date-8-9">
−
   <h1>Saturday, August 9</h1>
+
   <h1>Wednesday, August 9th</h1>
 
   <ul>
 
   <ul>
 
     <li>Cleaning of glassware, 10:00.</li>
 
     <li>Cleaning of glassware, 10:00.</li>
Line 577: Line 577:
  
 
<div id="date-8-10">
 
<div id="date-8-10">
−
   <h1>Sunday, August 10</h1>
+
   <h1>Thursday, August 10th</h1>
 
</div>
 
</div>
  
 
<div id="date-8-11">
 
<div id="date-8-11">
−
   <h1>Monday, August 11</h1>
+
   <h1>Friday, August 11th</h1>
 
   <ul>
 
   <ul>
 
     <li>Selection of plate&rsquo;s colonies to pass to Falcon tubes of 50 ml, 7:30</li>
 
     <li>Selection of plate&rsquo;s colonies to pass to Falcon tubes of 50 ml, 7:30</li>
Line 611: Line 611:
  
 
<div id="date-8-12">
 
<div id="date-8-12">
−
   <h1>Tuesday, August 12</h1>
+
   <h1>Saturday, August 12th</h1>
 
   <ul>
 
   <ul>
 
     <li>Making of electro-competent H5-&alpha;, 11:00.</li>
 
     <li>Making of electro-competent H5-&alpha;, 11:00.</li>
Line 623: Line 623:
  
 
<div id="date-8-13">
 
<div id="date-8-13">
−
   <h1>Wednesday, August 13</h1>
+
   <h1>Sunday, August 13th</h1>
 
   <ul>
 
   <ul>
 
     <li>Mini-prep of GFP, 12:00.</li>
 
     <li>Mini-prep of GFP, 12:00.</li>
Line 649: Line 649:
  
 
<div id="date-8-14">
 
<div id="date-8-14">
−
   <h1>Thursady, August 14</h1>
+
   <h1>Monday, August 14th</h1>
 
</div>
 
</div>
  
 
<div id="date-8-15">
 
<div id="date-8-15">
−
   <h1>Friday, August 15</h1>
+
   <h1>Tuesday, August 15th</h1>
 
</div>
 
</div>
  
 
<div id="date-8-16">
 
<div id="date-8-16">
−
   <h1>Saturday, August 16</h1>
+
   <h1>Wednesday, August 16th/h1>
 
   <ul>
 
   <ul>
 
     <li>Miniprep GFP, 12:00.</li>
 
     <li>Miniprep GFP, 12:00.</li>
Line 675: Line 675:
  
 
<div id="date-8-17">
 
<div id="date-8-17">
−
   <h1>Sunday, August 17</h1>
+
   <h1>Thursday, August 17th</h1>
 
   <ul>
 
   <ul>
 
     <li>Preparation of TAE buffer, 18:00.</li>
 
     <li>Preparation of TAE buffer, 18:00.</li>
Line 699: Line 699:
  
 
<div id="date-8-18">
 
<div id="date-8-18">
−
   <h1>Monday, August 18th.</h1>
+
   <h1>Friday, August 18th.</h1>
 
   <ul>
 
   <ul>
 
     <li><strong>Electrophoresis, 14:00.</strong></li>
 
     <li><strong>Electrophoresis, 14:00.</strong></li>
Line 717: Line 717:
  
 
<div id="date-8-19">
 
<div id="date-8-19">
−
   <h1>Tuesday, August 19</h1>
+
   <h1>Saturday, August 19th</h1>
 
   <ul>
 
   <ul>
 
     <li>MiniPrep GFP, 12:30</li>
 
     <li>MiniPrep GFP, 12:30</li>
Line 741: Line 741:
  
 
<div id="date-8-20">
 
<div id="date-8-20">
−
   <h1>Wednesday, August 20</h1>
+
   <h1>Sunday, August 20th</h1>
 
   <ul>
 
   <ul>
 
     <li>iGEM talk with interested students, 16:30.</li>
 
     <li>iGEM talk with interested students, 16:30.</li>
Line 760: Line 760:
  
 
<div id="date-8-21">
 
<div id="date-8-21">
−
   <h1>Thursday, August 21</h1>
+
   <h1>Monday, August 21st</h1>
 
   <ul>
 
   <ul>
 
     <li><strong>Teaching of how to make an electrophoresis gel to the newbies, 18:00.</strong></li>
 
     <li><strong>Teaching of how to make an electrophoresis gel to the newbies, 18:00.</strong></li>
Line 770: Line 770:
  
 
<div id="date-8-22">
 
<div id="date-8-22">
−
   <h1>Friday, August 22</h1>
+
   <h1>Tuesday, August 22nd</h1>
 
   <ul>
 
   <ul>
 
     <li>Teaching of how to make a transformation in calcium-competent cells, 17:00.</li>
 
     <li>Teaching of how to make a transformation in calcium-competent cells, 17:00.</li>
Line 800: Line 800:
  
 
<div id="date-8-23">
 
<div id="date-8-23">
−
   <h1>Saturday, August 23</h1>
+
   <h1>Wednesday, August 23rd</h1>
 
   <ul>
 
   <ul>
 
     <li><strong>Making of electro-competent cells, 9:00.</strong></li>
 
     <li><strong>Making of electro-competent cells, 9:00.</strong></li>
Line 822: Line 822:
  
 
<div id="date-8-24">
 
<div id="date-8-24">
−
   <h1>Sunday, August 24</h1>
+
   <h1>Thursday, August 24th</h1>
 
   <ul>
 
   <ul>
 
     <li><strong>Miniprep of the ligation D2-GFP, 11:00.</strong></li>
 
     <li><strong>Miniprep of the ligation D2-GFP, 11:00.</strong></li>
Line 848: Line 848:
  
 
<div id="date-8-25">
 
<div id="date-8-25">
−
   <h1>Monday, August 25</h1>
+
   <h1>Friday, August 25th</h1>
 
   <ul>
 
   <ul>
 
     <li><strong>Miniprep D2-GFP, 13:00.</strong></li>
 
     <li><strong>Miniprep D2-GFP, 13:00.</strong></li>
Line 874: Line 874:
  
 
<div id="date-8-26">
 
<div id="date-8-26">
−
   <h1>Tuesday, August 26</h1>
+
   <h1>Saturday, August 26th</h1>
 
</div>
 
</div>
  
Line 1,681: Line 1,681:
 
<!-- October -->
 
<!-- October -->
 
<div id="date-10-1">
 
<div id="date-10-1">
−
   <h1>Wednesday, October 1st.</h1>
+
   <h1>Sunday, October 1st.</h1>
 
   <ul>
 
   <ul>
 
     <li>Minipreps of all constructs, 12:00.</li>
 
     <li>Minipreps of all constructs, 12:00.</li>
Line 1,699: Line 1,699:
  
 
<div id="date-10-2">
 
<div id="date-10-2">
−
   <h1>Thursday, October 2nd.</h1>
+
   <h1>Monday, October 2nd.</h1>
 
   <ul>
 
   <ul>
 
     <li>Collaboration: gave C4 through C9 to the UANL team, 13:30.</li>
 
     <li>Collaboration: gave C4 through C9 to the UANL team, 13:30.</li>
Line 1,721: Line 1,721:
  
 
<div id="date-10-3">
 
<div id="date-10-3">
−
   <h1>Friday, October 3rd.</h1>
+
   <h1>Tuesday, October 3rd.</h1>
 
   <ul>
 
   <ul>
 
     <li>Digestions of C1, C2 and PET variants of the other constructs, 12:00.</li>
 
     <li>Digestions of C1, C2 and PET variants of the other constructs, 12:00.</li>
Line 1,743: Line 1,743:
  
 
<div id="date-10-4">
 
<div id="date-10-4">
−
   <h1>Saturday, October 4th.</h1>
+
   <h1>Wednesday, October 4th.</h1>
 
</div>
 
</div>
  
 
<div id="date-10-5">
 
<div id="date-10-5">
−
   <h1>Sunday, October 5th.</h1>
+
   <h1>Thursday, October 5th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Western Blot, 01:00.</li>
 
     <li>Western Blot, 01:00.</li>
Line 1,769: Line 1,769:
  
 
<div id="date-10-6">
 
<div id="date-10-6">
−
   <h1>Monday, October 6th.</h1>
+
   <h1>Friday, October 6th.</h1>
 
</div>
 
</div>
  
 
<div id="date-10-7">
 
<div id="date-10-7">
−
   <h1>Tuesday, October 7th.</h1>
+
   <h1>Saturday, October 7th.</h1>
 
</div>
 
</div>
  
 
<div id="date-10-8">
 
<div id="date-10-8">
−
   <h1>Wednesday, October 8th.</h1>
+
   <h1>Sunday, October 8th.</h1>
 
</div>
 
</div>
  
 
<div id="date-10-9">
 
<div id="date-10-9">
−
   <h1>Thursday, October 9th.</h1>
+
   <h1>Monday, October 9th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Minipreps for cells with flippase, 12:00.</li>
 
     <li>Minipreps for cells with flippase, 12:00.</li>
Line 1,803: Line 1,803:
  
 
<div id="date-10-10">
 
<div id="date-10-10">
−
   <h1>Friday, October 10th.</h1>
+
   <h1>Tuesday, October 10th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Digestions of Bba_1, Bba_2 and Bba_3, 08:00.</li>
 
     <li>Digestions of Bba_1, Bba_2 and Bba_3, 08:00.</li>
Line 1,825: Line 1,825:
  
 
<div id="date-10-11">
 
<div id="date-10-11">
−
   <h1>Saturday, October 11th.</h1>
+
   <h1>Wednesday, October 11th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Inoculations for constructs stocks, 20:00.</li>
 
     <li>Inoculations for constructs stocks, 20:00.</li>
Line 1,835: Line 1,835:
  
 
<div id="date-10-12">
 
<div id="date-10-12">
−
   <h1>Sunday, October 12th.</h1>
+
   <h1>Thursday, October 12th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Pictures taken for the wiki, 13:00.</li>
 
     <li>Pictures taken for the wiki, 13:00.</li>
Line 1,857: Line 1,857:
  
 
<div id="date-10-13">
 
<div id="date-10-13">
−
   <h1>Monday, October 13th.</h1>
+
   <h1>Friday, October 13th.</h1>
 
   <ul>
 
   <ul>
 
     <li>PCR for Red Lambda confirmation, 13:00.</li>
 
     <li>PCR for Red Lambda confirmation, 13:00.</li>
Line 1,883: Line 1,883:
  
 
<div id="date-10-14">
 
<div id="date-10-14">
−
   <h1>Tuesday, October 14th.</h1>
+
   <h1>Saturday, October 14th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Work on wiki</li>
 
     <li>Work on wiki</li>
Line 1,893: Line 1,893:
  
 
<div id="date-10-15">
 
<div id="date-10-15">
−
   <h1>Wednesday, October 15th.</h1>
+
   <h1>Sunday, October 15th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Work on wiki</li>
 
     <li>Work on wiki</li>
Line 1,903: Line 1,903:
  
 
<div id="date-10-16">
 
<div id="date-10-16">
−
   <h1>Thursday, October 16th.</h1>
+
   <h1>Monday, October 16th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Work on wiki</li>
 
     <li>Work on wiki</li>
Line 1,913: Line 1,913:
  
 
<div id="date-10-17">
 
<div id="date-10-17">
−
   <h1>Friday, October 17th.</h1>
+
   <h1>Tuesday, October 17th.</h1>
 
   <ul>
 
   <ul>
 
     <li>Work on wiki, its wiki-freeze day!!!</li>
 
     <li>Work on wiki, its wiki-freeze day!!!</li>

Revision as of 14:31, 6 July 2017

Thursday, June 1st.

Friday, June 2nd.

  • Lab cleanup, 12:00.
    • Everyone.

Saturday, June 3rd.

Sunday, June 4th.

Monday, June 5th.

Tuesday, June 6th.

  • iGEM 2014 meeting to talk about lab work, 14:00.
    • Everyone.

Wednesday, June 7th.

Thursday, June 8th.

Friday, June 9th.

Saturday, June 10th.

  • Revision of the protocols for next month’s lab-work, 12:00.
    • Everyone.
  • Revision of human practice ideas and collaborations with other teams, 13:00.
    • Everyone.

Sunday, June 11th.

  • Electro-competent cells, 13:00.
    • Cinthya

Monday, June 12th.

  • Sponsorships, all day.
    • Cinthya.

Tuesday, June 13th.

  • Meeting with advisors, 17:00.
  • Calcium-competent cells, 13:00.
    • Cinthya.

Wednesday, June 14th.

Thursday, June 15th.

Friday, June 16th.

  • Electro-competent cells, 14:00.
    • Cinthya, Mercedes.
  • Minipreps, 16:00.
    • Cinthya.
  • Electrophoresis, 19:00.
    • Cinthya.
  • Inoculations, 20:00.
    • Mercedes.

Saturday, June 17th.

Sunday, June 18th.

  • Electro-competent cells, 10:00.
    • Cinthya.
  • Minipreps, 13:00.
    • Mercedes.
  • Electrophoresis, 16:00.
    • Cinthya.
  • Inoculations, 20:00.
    • Cinthya.

Monday, June 19th.

Tuesday, June 20th.

  • Meeting with advisors, 17:00.

Wednesday, June 21st.

Thursday, June 22nd.

Friday, June 23rd.

  • Calcium-competent cells, 12:00.
    • Cinthya.

Saturday, June 24th.

  • Minipreps, 11:00
    • Mercedes.
  • Electrophoresis, 14:00.
    • Mercedes.
  • Inoculations. 18:00.
    • Mercedes.

Sunday, June 25th.

Monday, June 26th.

  • Minipreps, 10:00
    • Mercedes.
  • Electrophoresis, 13:30.
    • Mercedes.

Tuesday, June 27th.

  • Meeting with advisors, 17:00.

Wednesday, June 28th.

Thursday, June 29th.

Friday, June 30th.

Saturday, July 1st.

  • Practice: Minipreps, 13:00.
    • Eduardo Ramirez (1).
  • Practice: Ligations, 16:00.
    • Eduardo Ramirez (1).

Sunday, July 2nd.

Monday, July 3rd.

Tuesday, July 4th.

  • Minipreps, 12:00.
    • Mercedes.
  • Practice: Ligations, 15:00.
    • Eduardo Ramirez (1).
  • Practice: Electrophoresis, 17:00.
    • Eduardo Ramirez (1)

Wednesday, July 5th.

Thursday, July 6th.

Friday, July 7th.

  • Sendoffs of our teammates for a deserved vacation through the next 2 weeks.
  • Practice: Minipreps, 16:00.
    • Claudia.
  • Omar leaves for vacation.

Saturday, July 8th.

Sunday, July 9th.

Monday, July 10th.

  • Practice: Transformation of calcium-competent cells, 11:00,
    • Eduardo Ramirez (1), Claudia.
  • Inoculation on LB-agar plates, 13:00.
    • Claudia.
  • Laboratory summer cleaning, 15:00.
    • Claudia.

Tuesday, July 11th.

Wednesday, July 12th.

Thursday, July 13th.

Friday, July 14th.

Saturday, July 15th.

  • Practice: taking DNA out of the iGEM plates, 13:00.
    • Claudia.
  • Transformation with calcium-competent cells, 13:30.
    • Claudia.
  • Inoculations, 15:00.
    • Claudia.

Sunday, July 16th.

  • Minipreps, 12:00.
    • Eduardo Ramirez (1).
  • Ligations, 15:00.
    • Eduardo Ramirez (1).
  • Electrophoresis, 18:00.
  • Practice: DNA recovery from gel, 20:00.
    • Eduardo Ramirez (1)

Monday, July 17th.

Tuesday, July 18th.

Wednesday, July 19th.

  • Meeting with advisors to talk about gene problems and doubts, 17:00.

Thursday, July 20th.

Friday, July 21st.

Saturday, July 22nd.

  • Minipreps, 11:00.
    • Claudia.
  • Ligations, 15:00.
    • Claudia.
  • Electrophoresis, 19:00.
    • Claudia
  • Practice: DNA recovery from gel, 21:00.
    • Claudia.

Sunday, July 23rd.

Monday, July 24th.

Tuesday, July 25th.

Wednesday, July 26th.

  • Meeting with advisors, 17:00.

 

Monday, July 29th.

  • Meeting to plan the semester’s activities, 14:00.
    • Schedule of people in the lab.
    • Ways to integrate other students.
    • Events to talk about iGEM.

Thursday, July 27th.

Friday, July 28th.

Saturday, July 29th.

Sunday, July 30th.

Monday, July 31st.

Tuesday, August 1st.

Wednesday, August 2nd.

Thursday, August 3rd.

Friday, August 4th.

Saturday, August 5th.

Sunday, August 6th.

Monday, August 7th.

Tuesday, August 8th

  • Talk with new students about iGEM, 15:30.
    • Minerva, Mercedes, Cesar, Omar, Claudia, Eduardo Ramirez (1).
  • Session with advisors, 17:00.
    • Mercedes, Cesar, Omar, Claudia, Eduardo Ramirez (1).
  • Production of LB medium, 18:30.
    • Cesar.
  • Cleaning of glassware, 18:45.
    • Eduardo Ramirez (1).
  • Autoclave, 19:00.
    • Mercedes, Cesar, Eduardo Ramirez (1).
  • Grow H5-α culture, 21:00.
    • Cesar

Comments:  Most of the progress from today’s activities was not directly related to the laboratory. Keep in mind the session with the advisors next Friday (about the team rooster primarily) and the various events this month and the next with the general student population.

Wednesday, August 9th

  • Cleaning of glassware, 10:00.
  • Stock of the growth H5-α, 11:00.
  • Mini prep GFP, 15:00.
  • Calcium-competent of H5-α, 16:30.
  • Transformation of GFP (18C), GFP (18E), GFP (mini prep), RFP (O12), RFP (mini prep), (pLUX GFP). 9:40
    • Omar
  • Stock of the growth H5-α, 10:00.
    • Mercedes, Omar.
  • End of transformation (plaques), 23:23.
    • Omar, Mercedes.

Thursday, August 10th

Friday, August 11th

  • Selection of plate’s colonies to pass to Falcon tubes of 50 ml, 7:30
    • Mercedes.
  • Miniprep, 14:00
    • Omar
  • Digestions, 19:00.
    • Cesar
  • Electrophoresis, 21:00.
    • Cesar.

Saturday, August 12th

  • Making of electro-competent H5-α, 11:00.
    • Mercedes.

Sunday, August 13th

  • Mini-prep of GFP, 12:00.
    • Before incubation: Minerva.
    • After incubation: Omar, Claudia.
  • Transformation of the electro-competent cells with RFP, 17:00.
    • Eduardo Ramirez (1), Claudia, Omar.
  • Plating of the transformed cells, 21:00.
    • Eduardo Ramirez (1).
  • Electrophoresis of GFP mini-preps and digestions of GFP (Kn) and GFP (CAM), 21:10.
    • Cesar, Eduardo Cepeda.
      • Failed.

Monday, August 14th

Tuesday, August 15th

Wednesday, August 16th/h1>
  • Miniprep GFP, 12:00.
    • Mercedes.
  • Digestions, 15:30.
    • Mercedes.

Thursday, August 17th

  • Preparation of TAE buffer, 18:00.
    • Mercedes.
  • Digestions, 20:00.
    • Mercedes.
  • Electrophoresis, 22:00.
    • Mercedes, Cesar, Claudia, Eduardo Ramirez (2), Omar.

Friday, August 18th.

  • Electrophoresis, 14:00.
    • Minerva.
  • Teaching of the newbies (Miniprep basics), 16:00.
    • Mercedes.
  • Autoclave of general materials, 18:00.
    • Everyone.

Saturday, August 19th

  • MiniPrep GFP, 12:30
    • Mercedes
  • Digestions, 18:00
    • Eduardo Cepeda
  • Electrophoresis, 21:00
    • Newbies (Alex, Dany).

Sunday, August 20th

  • iGEM talk with interested students, 16:30.
    • Special Guest: Ana Sifuentes
  • Teaching of how to make media to newbies, 18:00.
    • Claudia.

Monday, August 21st

  • Teaching of how to make an electrophoresis gel to the newbies, 18:00.
    • Claudia.

Tuesday, August 22nd

  • Teaching of how to make a transformation in calcium-competent cells, 17:00.
    • Claudia.
  • Purification of the Minipreps of GFP and the promoters D2 and D3, 18:00.
    • Eduardo Ramirez (2), Omar.
  • Electrophoresis of the past purifications, 22:00.
    • Mercedes.
  • Purification of the electrophoresis and ligations, 23:30.
    • Mercedes, Eduardo Cepeda.

Wednesday, August 23rd

  • Making of electro-competent cells, 9:00.
    • Mercedes.
  • Visit to UANL to meet their iGEM team and to hear the iGEM HQ talk, 13:30.
    • Mercedes, Claudia, Eduardo Ramirez (1), Eduardo Cepeda.
  • Transformations of the electro-competent cells with D2, D3 and GFP, 18:00.
    • Claudia.
  • Inoculation of the ligation D2-GFP, 20:00.
    • Eduardo Cepeda.

Thursday, August 24th

  • Miniprep of the ligation D2-GFP, 11:00.
    • Minerva.
  • Digestion of D2, D3, GFP and RFP, 16:00.
    • Minerva, Eduardo Cepeda.
  • Electrophoresis, 19:00.
    • Minerva, Eduardo Cepeda.
  • DNA purification of the gel, 20:15.
    • Mercedes.
  • Ligations D2-GFP, D3-GFP, 21:00.
    • Mercedes.

Friday, August 25th

  • Miniprep D2-GFP, 13:00.
    • Eduardo Cepeda.
  • Digestion of D2, 16:00.
    • Eduardo Cepeda.
  • Inoculation practice with the newbies, 19:00.
    • Claudia.
  • Transformations with electro-competent cells, 21:30.
    • Cesar.
  • Preparation of plaques, 23:00.
    • Eduardo Cepeda.

Saturday, August 26th

Wednesday, August 27

  • Inoculm of BL21 7:00.
    • Eduardo Cepeda.
  • Miniprep D2-GFP, D3-GFP, D2-RFP, D3-RFP, 13:30.
    • Eduardo Cepeda.
  • Digestions, 18:00.
    • Mercedes.
  • Electrophoresis, 20:00
    • Mercedes.
  • Preparation of calcium-competents BL21,
    • Claudia, Eduardo Ramirez.

Thursday, August 28

  • Electrophoresis, 22:00.
    • Newbies.
  • Preparation of calcium-competent cells TOP10, 22:00.
    • Eduardo Ramirez (2).

Friday, August 29

  • Preparation of chloramphenicol antibiotic, 10:00.
    • Mercedes.
  • Miniprep of D2-GFP and D3-GFP, 13:00.
    • Omar, Minerva.
  • Miniprep of D2 and D3 promoters, 15:00.
    • Omar.
  • Purification of the minipreps, 20:00.
    • Mercedes.
  • ?

Saturday, August 30

Sunday, August 31

  • Miniprep GFP Kn, RFP, 3 replicas of GFP Cam, 15:00.
    • Claudia, Eduardo Ramirez (2).
  • Digestions, 18:30.
    • Mercedes.
  • Electrophoresis, 22:40.
    • Omar.

Monday, September 1st.

  • Apoptosis kit arrived!, 14:00.
  • Accutase arrived!, 14:00.
  • Genes arrived!, 14:00.
  • Preparation of LB and LB-agar media, 13:30.
    • Eduardo Ramirez (1).
  • Preparation of the plaques for the transformation, 17:00.
    • Eduardo Cepeda.

Abbreviation

Construct

Gene

Resistance

LPP

C1

LPP Red Lambda

Amp

MSBB

C2

Red Lambda

Kn + Amp

H-GFP

C4

GFP

Amp

C-Apop

C5

Apoptine

Amp

H-SiRNA 1

C6

SiRNA 1

Amp

H-SiRNA 2

C7

SiRNA 2

Amp

H-SiRNA 3

C8

SiRNA 3

Amp

H-SiRNA-Apop

C9

SiRNA + Apoptine

Amp

BBa_1

Biobrick

  

Cam

BBa_2

Biobrick

  

Cam

BBa_3

Biobrick

Peptide + p3

Cam
  • HyperLadder 50bp arrived!, 17:10
  • HLA arrived!, 17:10:
  • Transformation of C1 through C7 in TOP10, 19:00.
    • Minerva.
  • Transformation of GFP and RFP in TOP10, 21:30.
    • Mercedes.

Tuesday, September 2

  • Inoculation of plates with transformed colonies from yesterday, 12:00.
    • Minerva.
  • Transformations of GFP Amp, RFP Cam, D1 Kn, D2 Cam, D3 Amp, 19:30.
    • Eduardo Ramirez (2).
  • Miniprep of the grown transformed cells from yesterday (C4, C5, C6, C7, D2, GFP, D1, BBa_1), 21:00.
    • Eduardo Cepeda.

Wednesday, September 3

  • Digestions of the last minipreps, 02:00.
    • Eduardo Cepeda.
  • Electrophoresis, 03:30.
    • Eduardo Cepeda.
  • Preparation of electro-competent TOP10 cells, 08:00.
    • Newbies: Alex, Danny, Chuy, Caro.
  • Preparation of stock cells (C4, C5, C6, C7, D2, GFP, D1, BBa_1), 18:30.
    • Mercedes.
  • Ligations of the C4, C5, C6, C7 with the pcb1c3, 20:30.
    • Eduardo Cepeda.
  • Inoculation of TOP10 for electro-competent cells tomorrow and D2 cells, 01:25.
    • Mercedes.

Thursday, September 4

Friday, September 5

  • Preparation of the tickets for the cake raffle (fundraising), 12:00.
    • Cinthya, Omar, Eduardo Ramirez (1), Eduardo Cepeda, Mercedes.
  • Miniprep, 14:00.
    • Mercedes and Eduardo Cepeda with special guest: Ana Cristina.
  • Talk with Professor Cardineau about the legislation proposal, 17:00.
    • Omar.
  • Preparations of electro-competente TOP10 cells, 17:30.
    • Eduardo Ramirez (2), Mercedes.
  • Digestions, 19:00.
    • Eduardo Cepeda.
  • Inoculation of LB-agar plaques, 21:00.
    • Cynthia.

Saturday, September 6

Sunday, September 7

Monday, September 8

Tuesday, September 9

  • Inoculation of PCB1c3 (with C4, C5, C7 separately), 13:30.
    • Eduardo Cepeda.

Wednesday, September 10

Thursday, September 11

  • Revision of (not grown yet) plates, 12:00.
    • Eduardo Cepeda.
  • Inoculaton of D2-GFP, 21:00.
    • Eduardo Cepeda.

Friday, September 12th

  • Cleaning of the electroporation cells for the electro-competent cells, 16:00.
    • Minerva.
  • Inoculation of cells that grew from the prior day, 18:00.
    • Cinthya.

Saturday, September 13

  • Transformation of the D2 and D3 variants for the interlab project, 00:10.
    • Eduardo Ramirez (2), Mercedes, Claudia, Omar.

Sunday, September 14th.

  • Miniprep, 17:00.
    • Mercedes, Eduardo Ramirez (2)
  • Sterilization of Falcon tubes, LB broth and plastic pearls, 18:30.
    • Omar
  • Digestions, 21:00.
    • Mercedes.
  • Inoculation of the transformed cells with D2 and D3 variants, 22:00.
    • Omar.

Monday, September 15

  • Miniprep of yesterday's inoculations of D2 and D3 variants, 10:00.
    • Mercedes.
  • Digestions, 17:30.
    • Eduardo Ramirez (2).
  • Inoculation of TOP10 and XL-Blue (Tet), 22:50.
    • Mercedes.

Tuesday, September 16th.

  • Transformations of the M13 bacteriophage into the XL-Blue, 10:00.
    • Mercedes.

Wednesday, September 17

  • Miniprep 01:15.
    • Minerva, Eduardo Cepeda.
  • Digestions (C4, C5, C6, C7 and , 02:53.
    • Eduardo Cepeda.
  • PBS buffer, 12:30.
    • Omar.
  • Recovery of DNA from Gel, 13:30.
    • Eduardo Cepeda.

Thursday, September 18

  • Transformations of C8, C9 and ligation in TOP10 electro-competent, 00:40.
    • Cinthya, Omar.
  • Electrophoresis, 01:30.
    • Mercedes, Eduardo Ramirez (1).
  • Plates from the transformations, 01:50.
    • Omar.
  • Miniprep of PET, 12:30.
    • Eduardo Cepeda.
  • Transformation replica of the same ligation as before, 15:30.
    • Omar.
  • Preparation of LB broth, 17:00.
    • All the newbies (Caro, Jesus, Alex, Dany).
  • Digestion of PET, 19:30.
    • Claudia.

Friday, September 19

  • Ligation of PET with all the constructs, 01:19.
    • Eduardo Cepeda.
  • Electrophoresis, 03:30.
    • Eduardo Cepeda.
  • Miniprep, 8:30.
    • Mercedes.
  • Digestions, 13:00.
    • Mercedes.
  • Electrophoresis, 15:30.
    • Mercedes.
  • Quantification of the fluorescence for the interlab project, 16:30.
    • Mercedes, Eduardo Ramirez (2).
  • Group photo taken, 18:00.
    • Everyone except Mercedes, Eduardo Cepeda and Eduardo Ramirez.
  • Group video filmed, 18:30.
    • Everyone.
  • Transformation of TOP10 cells with PET + (C4, C5, C6, C7), 20:30.
    • Cinthya, Eduardo Cepeda.
  • Transformation of TOP10 cells with various repetitions of D2 and D3 (with GFP or RFP), 21:00.
    • Mercedes, Eduardo Ramirez (2).
  • Inoculation in Falcon tubes with the ligation of C5 and various others, 22:00.
    • Omar.
  • Inoculation in plates of the previous transformed TOP10 cells with PET, 23:00.
    • Cinthya.

Saturday, September 20

  • Transformation in electro-competent cells, 01:20.
    • Mercedes, Eduardo Ramirez.
  • Minipreps, 18:00.
    • Mercedes, Omar.
  • Digestions, 22:00.

Sunday, September 21st.

  • Electrophoresis, 00:00.
    • Mercedes.
  • Inoculations for minipreps, 01:00.
    • Mercedes.
  • Minipreps,
    • Eduardo Cepeda.

Monday, September 22

  • Miniprep, 08:00.
    • Mercedes.
  • Revision of the cell stocks, contamination detected, 09:00.
    • Mercedes.
  • Inoculations of C8 and C9, 22:00.
    • Eduardo Cepeda.

Tuesday, September 23

  • Preparation of electo-competent cells DH5a, 03:00.
    • Minerva.
  • Minipreps for the interlab, 06:00
    • Mercedes, Minerva.
  • Transformations for the interlab, 07:30.
    • Mercedes, Minerva.
  • Digestions for the interlab, 12:30.
    • Mercedes.
  • Miniprep, 13:30.
    • Eduardo Cepeda.
  • Electrophoresis for interlab, 19:00.
    • Eduardo Ramirez (2)
  • Electrophoresis for the project (C8, C9), 21:00.
    • Eduardo Ramirez (2)

Wednesday, September 24

  • Purification of the electrophoresis gel, 01:20.
    • Mercedes.
  • Digestions for the interlab, 01:20.
    • Eduardo Cepeda.
  • Ligations, 02:00.
    • Eduardo Cepeda.
  • Electrophoresis for the interlab, 02:40.
    • Mercedes.
  • Inoculations for the interlab, 11:00.
    • Omar, Mercedes.

Thursday, September 25

  • Inoculation of XL-Blue, 22:00.
    • Mercedes.
  • Infection of the XL-Blue with bacteriophages, 23:00.
    • Mercedes.

Friday, September 26

  • Miniprep of the bacteriophage-infected cells, 03:00.
    • Mercedes.
  • Preparation of the cells for quantification of fluorescence and OD for the interlab project, from 8:00 to 24:00.
    • Mercedes.
  • Purification of the miniprep. 18:00.
    • Eduardo Ramirez (1), Claudia.
  • Transformations of C1, C2, Bba_1, Bba_2, and ligations, 19:00.
    • Cinthya, Omar.
  • Plating of the transformations, 22:00.
    • Cinthya.

Saturday, September 27

  • Inoculation of C1, C2, Bba_1, Bba _2 and ligations, 20:00.
    • Omar
  • Sterilization of material, 22:00.
    • Omar.

Sunday, September 28

  • Preparation of electro-competent cells, 01:00.
    • Eduardo Cepeda.
  • Ligations, 03:00.
    • Eduardo Cepeda.
  • Electrophoresis, 12:00.
    • Eduardo Cepeda.
  • Minipreps of the inoculations from Saturday, 13:00.
    • Claudia, Eduardo Ramirez (1).
  • Recovery of DNA from gel, 15:00.
    • Claudia.

Monday, September 29

Tuesday, September 30

Sunday, October 1st.

  • Minipreps of all constructs, 12:00.
    • Eduardo.
  • Digestions of all constructs, 20:30.
    • Claudia, Jesus.
  • Miniprep of C2, 21:00.
    • Jesus.

Monday, October 2nd.

  • Collaboration: gave C4 through C9 to the UANL team, 13:30.
    • Mercedes.
  • Inoculo XL-Blue, 18:00.
    • Eduardo Ramirez (2).
  • Miniprep C8, C10, Bb_1, C6, 16:30.
    • Eduardo Cepeda, Eduardo Ramirez (2).
  • Miniprep of phagemides, 18:30.
    • Eduardo Cepeda.

Tuesday, October 3rd.

  • Digestions of C1, C2 and PET variants of the other constructs, 12:00.
    • Eduardo Cepeda.
  • Minipreps, 12:00.
    • Claudia.
  • Electrophoresis, 16:00.
    • Eduardo Cepeda.
  • Inoculations, 20:00.
    • Mercedes.

Wednesday, October 4th.

Thursday, October 5th.

  • Western Blot, 01:00.
    • Minerva.
  • Minipreps, 00:30.
    • Eduardo Cepeda.
  • Electrophoresis, 04:00.
    • Eduardo Cepeda.
  • Inoculations, 18:00.
    • Eduardo Cepeda.

Friday, October 6th.

Saturday, October 7th.

Sunday, October 8th.

Monday, October 9th.

  • Minipreps for cells with flippase, 12:00.
    • Eduardo Cepeda.
  • Stock of cells with flippase, 12:00.
    • Mercedes.
  • Digestions for flippase, 16:00.
    • Eduardo Cepeda.
  • Electrophoresis, 18:00.
    • Eduardo Cepeda.

Tuesday, October 10th.

  • Digestions of Bba_1, Bba_2 and Bba_3, 08:00.
    • Eduardo Cepeda.
  • Digestion of the modified phage, 09:00.
    • Mercedes.
  • Electrophoresis, 12:00.
    • Eduardo Ramirez (2).
  • Ligation of the phages back to circle form, 13:00.
    • Mercedes.

Wednesday, October 11th.

  • Inoculations for constructs stocks, 20:00.
    • Mercedes.

Thursday, October 12th.

  • Pictures taken for the wiki, 13:00.
    • Everyone.
  • PCR, 16:00.
    • Eduardo Cepeda.
  • Stocks, 18:00.
    • Mercedes.
  • Electrophoresis, 20:00.
    • Eduardo Cepeda.

Friday, October 13th.

  • PCR for Red Lambda confirmation, 13:00.
    • Eduardo Cepeda.
  • Electrophoresis, 15:00.
    • Mercedes.
  • Digestions, 16:00.
    • Mercedes.
  • TAE buffer preparation, 20:00.
    • Newbies: Alejandro, Danny.
  • Western Blott, 21:00.
    • Minerva, Claudia.

Saturday, October 14th.

  • Work on wiki
    • Sebastián Valdivieso, Eduardo Zardain, Eduardo Ramírez

Sunday, October 15th.

  • Work on wiki
    • Sebastián Valdivieso, Eduardo Zardain, Eduardo Ramírez

Monday, October 16th.

  • Work on wiki
    • Sebastián Valdivieso, Eduardo Zardain, Eduardo Ramírez

Tuesday, October 17th.

  • Work on wiki, its wiki-freeze day!!!
    • Sebastián Valdivieso, Eduardo Zardain, Eduardo Ramírez