Difference between revisions of "Team:DTU-Denmark/Biobrick Tutorial"

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             <div class="colp-10 rmvpadmarg">  
 
             <div class="colp-10 rmvpadmarg">  
 
              
 
              
−
                 <h1 class="bottomborder">Biobrick Tutorial 2017</h1>
+
                 <h1 class="bottomborder">BioBrick Tutorial 2017</h1>
 
                  
 
                  
−
                 <p>On Friday of the second weekend of March (9th-11.th of march), DTU BioBuilders stood in anticipation for the first arrival of the invited Nordic iGEM teams  
+
                 <p>On Friday of the second weekend of March (9th-11th of March) the DTU BioBuilders stood in anticipation for the first arrival of the invited Nordic iGEM teams.</p>
−
</p>
+
<p><br>
−
<p>
+
The following teams marked their presence:<br>
−
Teams from Norway: Universitetet i Oslo, UiO, Sweden: Chalmers University of Technology , and Denmark: University of Copenhagen, (KU) and University of Southern Denmark, (SDU) marked their presence. Counting 44 people in all made this year’s biobrick tutorial the most attended as of yet.
+
&emsp; - Norway: Universitetet i Oslo (UiO) <br>
 +
&emsp; - Sweden: Chalmers University of Technology <br>
 +
&emsp; - Denmark: University of Copenhagen (KU) and University of Southern Denmark (SDU)<br>
 +
Counting 44 people in total made this year’s BioBrick tutorial the most attended to date.
 
</p>
 
</p>
  
−
<img src="https://static.igem.org/mediawiki/2017/0/0a/T--DTU-Denmark--BT_picture_of_teams.jpg" alt="Photo of participants">
+
<div class="imgdisplay">
 +
    <img src="https://static.igem.org/mediawiki/2017/0/0a/T--DTU-Denmark--BT_picture_of_teams.jpg" alt="Photo of participants">
 +
</div>
  
 
<P>
 
<P>
−
The purpose of the Biobrick tutorial is to demonstrate the modularity and versatility of the Biobrick beyond on a theoretical level, to socialize and initiate a foundation for further collaboration and to lower the distance between the registry and the members of the respective teams - from teams to start exploring the registry. And to get a clearer idea of the  
+
The purpose of the BioBrick tutorial is to demonstrate the modularity and versatility of the BioBricks beyond a theoretical level, to socialize and initiate a foundation for further collaboration and to lower the distance between the registry and the members of the respective teams - from teams to start exploring the registry. And to get a clearer idea of the  
 
concepts of  “Digestion”, “Ligation”, “transformation” and “plate spreading” keeping in mind that the members of the teams have different academic backgrounds and are enrolled in various programs.
 
concepts of  “Digestion”, “Ligation”, “transformation” and “plate spreading” keeping in mind that the members of the teams have different academic backgrounds and are enrolled in various programs.
−
</p>
+
</p><br>
  
 
<p>
 
<p>
−
Thus, the bulk of the weekend (Saturday) was spent getting hand-on experience with the 3A assembly. This was achieved in the form of a laboratory exercise, where different genetical parts were combined by small teams of four. We achieved this by combining the BioBrick plasmid backbone (pSB1C3)  with a purple-blue chromoproteins (RBS-amilCP) under control of a constitutive promoter.
+
Thus, the bulk of the weekend (Saturday) was spent getting hand-on experience with the 3A assembly. This was achieved in the form of a laboratory exercise, where different genetical parts were combined by small teams of four. We achieved this by combining the BioBrick plasmid backbone (pSB1C3)  with a purple-blue chromoproteins (RBS-amilCP) under control of a constitutive promoter.<br>
 +
To view the protocol, click <a href="https://static.igem.org/mediawiki/2017/b/b4/T--DTU-Denmark--protocol_bbt.pdf">here</a>.
 
</p>
 
</p>
  
−
<img src="https://static.igem.org/mediawiki/2017/9/96/T--DTU-Denmark--BT_plate1.jpg" alt="Plate example">
+
<div class="imgdisplay">
 +
    <img src="https://static.igem.org/mediawiki/2017/9/96/T--DTU-Denmark--BT_plate1.jpg" alt="Plate example">
 +
</div>
  
 
<p>
 
<p>
−
Small lectures was interspersed in between incubation times. Lectures were prepared by us, the team form KU and by invited speakers. These lectures covered technical topics such as cloning techniques: Golden gate and USER cloning, a lecture contributed the supervisors of the iGEM-Copenhagen team - a runthrough of the registry and interactive sessions on communicating science effectively to an audience of peers. A full overview of the interesting events can be found on the bottom of this page.
+
Small lectures was interspersed in between incubation times. Lectures were prepared by us, the team form KU and by invited speakers. These lectures covered technical topics such as cloning techniques: Golden gate and USER cloning, a lecture contributed the supervisors of the iGEM-Copenhagen team - a runthrough of the registry and interactive sessions on communicating science effectively to an audience of peers. A full overview of the interesting events can be found on the bottom of this page.<br><br>
 
</p>
 
</p>
  
 
<p>
 
<p>
−
On Sunday, we arranged for a panel of iGEM alumni and our supervisor to take and answer questions that the teams had. Questions covered topics on funding- and PR activities, judging criterias and team dynamics. This was followed by an activity encouraging ideation in the form of an exercise, where the teams built a bridge between a gap of the tables after brief deliberation among themselves on the design features and load requirements.
+
On Sunday, we arranged for a panel of iGEM alumni and our supervisor Chris Workman to take and answer questions that the teams had. Questions covered topics on funding- and PR activities, judging criterias and team dynamics. This was followed by an activity encouraging ideation in the form of an exercise, where the teams built a bridge between a gap of the tables after brief deliberation among themselves on the design features and load requirements.<br><br>
 
</p>
 
</p>
  
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−
<h2 id="overview">Overview of the Tutorial</h2>
+
<h2 id="overview" class="bottomborder" align="center">Overview of the Tutorial</h2>
  
 
<figure>
 
<figure>
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         </tr>
 
         </tr>
 
         <tr>
 
         <tr>
−
             <td>1</td>
+
             <td>Morning</td>
−
             <td>2</td>
+
             <td>-</td>
−
             <td>3</td>
+
            <td>
 +
                <span>Lecture 2: "3A assembly"</span><br>
 +
                <span>Lab: Digestion of Biobricks</span><br>
 +
            </td>
 +
             <td>
 +
                <span>Lab: Evaluating results</span><br>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td>Noon</td>
 +
            <td>-</td>
 +
            <td>
 +
                <span>Lunch</span><br>
 +
                <span>Lab: Heat activation & ligation</span><br>
 +
            </td>
 +
            <td>
 +
                <span>Panel discussion</span><br>
 +
                <span>Lunch</span><br>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td>Afternoon</td>
 +
            <td>Arrival of teams</td>
 +
            <td>
 +
                <span>Lecture 3: "Golden Gate and USER Cloning"</span><br>
 +
                <span>Lab: Transformation</span><br>
 +
                <span>Lecture 4: "Anatomy of the elevator pitch"</span><br>
 +
                <span>Pitching exercise</span><br>
 +
            </td>
 +
            <td>
 +
                <span>Lecture 6: "Prototyping"</span><br>
 +
                <span>Bridgebuilding: Build your own prototype</span><br>
 +
                <span>Awardshow</span><br>
 +
                <span>Wrap up and evaluation</span><br>
 +
            </td>
 +
        </tr>
 +
        <tr>
 +
            <td>Evening</td>
 +
            <td>
 +
                <span>Introduction and safety tour of the laboratories</span><br>
 +
                <span>Lecture 1: "What is a BioBrick and the Registry"</span><br>
 +
                <span>Dinner and socializing</span>
 +
            </td>
 +
            <td>
 +
                <span>Lecture 5: "Communicate your message"</span><br>
 +
                <span>Lab: Inoculation and plating</span><br>
 +
                <span>Dinner & socializing</span><br>
 +
            </td>
 +
            <td>-</td>
 
         </tr>
 
         </tr>
 
    
 
    
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     <figcaption></figcaption>
 
     <figcaption></figcaption>
 
</figure>
 
</figure>
 +
 +
<h2 id="gallery" class="bottomborder" align="center" style="clear: both;">Gallery</h2>
 +
<div class="row">
 +
            <div class="colp-4 rmvpadmarg">
 +
                <figure>
 +
                    <div class="galleryimg">
 +
                        <img src="https://static.igem.org/mediawiki/2017/c/cc/T--DTU-Denmark--BT_labpic1.jpg" alt="Lab picture">
 +
                    </div>
 +
                    <figcaption>Concentrated work in the laboratory</figcaption>
 +
                </figure>
 +
            </div>
 +
 +
            <div class="colp-2 rmvpadmarg" style="white-space: pre;">
 +
                <p> </p>
 +
            </div>
 +
 +
            <div class="colp-4 rmvpadmarg">
 +
                <figure>
 +
                    <div class="galleryimg">
 +
                        <img src="https://static.igem.org/mediawiki/2017/8/8e/T--DTU-Denmark--BT_lecture1.jpg" alt="Lecture">
 +
                    </div>
 +
                    <figcaption>Focused listening during lecture on Golden Gate and USER cloning techniques</figcaption>
 +
                </figure>
 +
            </div>
 +
</div>
 
              
 
              
 +
 +
<div class="row">
 +
            <div class="colp-4 rmvpadmarg">
 +
                <figure>
 +
                <div class="galleryimg">
 +
                    <img src="https://static.igem.org/mediawiki/2017/f/fa/T--DTU-Denmark--BT_panel_discussion.jpg" alt="Panel discussion">
 +
                </div>
 +
                <figcaption>Panel Discussion with iGEM alumni and DTU Biobuilders' supervisor Chris Workman</figcaption>
 +
                </figure>
 +
            </div>
 +
 +
            <div class="colp-2 rmvpadmarg" style="white-space: pre;">
 +
                <p> </p>
 +
            </div>
 +
 +
            <div class="colp-4 rmvpadmarg">
 +
                <figure>
 +
                <div class="galleryimg">
 +
                    <img src="https://static.igem.org/mediawiki/2017/7/76/T--DTU-Denmark--BT_laughlab.jpg" alt="Prototype Lecture">
 +
                </div>
 +
                <figcaption>You can have fun while working seriously in the laboratory!</figcaption>
 +
                </figure>
 +
            </div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 
             </div>
 
             </div>
 
              
 
              
Line 93: Line 211:
 
                         <li  class="rightnavbarbtn">
 
                         <li  class="rightnavbarbtn">
 
                             <a href="#biobricktutorial">Biobrick Tutorial 2017</a>
 
                             <a href="#biobricktutorial">Biobrick Tutorial 2017</a>
 +
                        </li>
 +
                        <li  class="rightnavbarbtn">
 +
                            <a href="#overview">Event Schedule</a>
 +
                        </li>
 +
                        <li  class="rightnavbarbtn">
 +
                            <a href="#gallery">Gallery</a>
 
                         </li>
 
                         </li>
 
                     </ul>
 
                     </ul>

Latest revision as of 20:09, 1 November 2017

BioBrick Tutorial 2017

On Friday of the second weekend of March (9th-11th of March) the DTU BioBuilders stood in anticipation for the first arrival of the invited Nordic iGEM teams.


The following teams marked their presence:
  - Norway: Universitetet i Oslo (UiO)
  - Sweden: Chalmers University of Technology
  - Denmark: University of Copenhagen (KU) and University of Southern Denmark (SDU)
Counting 44 people in total made this year’s BioBrick tutorial the most attended to date.

Photo of participants

The purpose of the BioBrick tutorial is to demonstrate the modularity and versatility of the BioBricks beyond a theoretical level, to socialize and initiate a foundation for further collaboration and to lower the distance between the registry and the members of the respective teams - from teams to start exploring the registry. And to get a clearer idea of the concepts of “Digestion”, “Ligation”, “transformation” and “plate spreading” keeping in mind that the members of the teams have different academic backgrounds and are enrolled in various programs.


Thus, the bulk of the weekend (Saturday) was spent getting hand-on experience with the 3A assembly. This was achieved in the form of a laboratory exercise, where different genetical parts were combined by small teams of four. We achieved this by combining the BioBrick plasmid backbone (pSB1C3) with a purple-blue chromoproteins (RBS-amilCP) under control of a constitutive promoter.
To view the protocol, click here.

Plate example

Small lectures was interspersed in between incubation times. Lectures were prepared by us, the team form KU and by invited speakers. These lectures covered technical topics such as cloning techniques: Golden gate and USER cloning, a lecture contributed the supervisors of the iGEM-Copenhagen team - a runthrough of the registry and interactive sessions on communicating science effectively to an audience of peers. A full overview of the interesting events can be found on the bottom of this page.

On Sunday, we arranged for a panel of iGEM alumni and our supervisor Chris Workman to take and answer questions that the teams had. Questions covered topics on funding- and PR activities, judging criterias and team dynamics. This was followed by an activity encouraging ideation in the form of an exercise, where the teams built a bridge between a gap of the tables after brief deliberation among themselves on the design features and load requirements.

We had fun doing this and are handing the baton to the next year’s DTU BioBuilders team to make the Biobrick tutorial 2018, at least as successful as it was this year.

Overview of the Tutorial

Friday Saturday Sunday
Morning - Lecture 2: "3A assembly"
Lab: Digestion of Biobricks
 Lab: Evaluating results
Noon - Lunch
Lab: Heat activation & ligation
 Panel discussion
Lunch
Afternoon Arrival of teams Lecture 3: "Golden Gate and USER Cloning"
Lab: Transformation
Lecture 4: "Anatomy of the elevator pitch"
Pitching exercise
 Lecture 6: "Prototyping"
Bridgebuilding: Build your own prototype
Awardshow
Wrap up and evaluation
Evening Introduction and safety tour of the laboratories
Lecture 1: "What is a BioBrick and the Registry"
Dinner and socializing 		Lecture 5: "Communicate your message"
Lab: Inoculation and plating
Dinner & socializing
 -
Lab picture
Concentrated work in the laboratory

Lecture
Focused listening during lecture on Golden Gate and USER cloning techniques
Panel discussion
Panel Discussion with iGEM alumni and DTU Biobuilders' supervisor Chris Workman

Prototype Lecture
You can have fun while working seriously in the laboratory!

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