Difference between revisions of "Team:Rice/Parts"
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<td><a href='http://parts.igem.org/Part:BBa_K2194000' style='color:rgb(153,117,185)'><font color='Indigo '>BBa_K2194000</font></a></td> | <td><a href='http://parts.igem.org/Part:BBa_K2194000' style='color:rgb(153,117,185)'><font color='Indigo '>BBa_K2194000</font></a></td> | ||
| − | <td> | + | <td>chrR6 Chromate Reductase Enzyme</td> |
<td>basic</td> | <td>basic</td> | ||
<td width = "50%"><img src="https://static.igem.org/mediawiki/parts/4/40/ChrR6_yellow.svg" style="height:70%; width:20%;"> | <td width = "50%"><img src="https://static.igem.org/mediawiki/parts/4/40/ChrR6_yellow.svg" style="height:70%; width:20%;"> | ||
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<tr> | <tr> | ||
<td><a href='http://parts.igem.org/Part:BBa_K2194002' style='color:rgb(153,117,185)'><font color='Indigo '>BBa_K2194002</font></a></td> | <td><a href='http://parts.igem.org/Part:BBa_K2194002' style='color:rgb(153,117,185)'><font color='Indigo '>BBa_K2194002</font></a></td> | ||
| − | <td width = "20%">PgntK Negative Feedback Membrane Stress Promoter </td> | + | <td width = "20%"><i>PgntK</i> Negative Feedback Membrane Stress Promoter </td> |
<td>basic</td> | <td>basic</td> | ||
| − | <td><img src="https://static.igem.org/mediawiki/parts/d/d0/Rice_PgntK_without_RBS.svg" style="height:70%; width:20%;" > <p> The PgntK promoter is a native Escherichia coli negative feedback membrane stress promoter.</p> </td> | + | <td><img src="https://static.igem.org/mediawiki/parts/d/d0/Rice_PgntK_without_RBS.svg" style="height:70%; width:20%;" > <p> The <i>PgntK</i> promoter is a native <i>Escherichia coli</i> negative feedback membrane stress promoter. BioBrick BBa_K2194002 contains the 200 bp upstream of the <i>gntK</i> gene in <i>Escherichia coli</i>, which has been previously experimentally shown to incorporate the full <i>Pgntk</i> promoter region.</p> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href='http://parts.igem.org/Part:BBa_K2194001' style='color:rgb(153,117,185)'><font color='Indigo '>BBa_K2194001</font></a></td> | <td><a href='http://parts.igem.org/Part:BBa_K2194001' style='color:rgb(153,117,185)'><font color='Indigo '>BBa_K2194001</font></a></td> | ||
| − | <td> | + | <td>Sulfate Binding Protein (sbp) cds</td> |
<td>basic</td> | <td>basic</td> | ||
| − | <td><img src="https://static.igem.org/mediawiki/parts/5/57/Rice_sbp.svg" style="height:30%; width:20%;"> <p> The sbp protein is found in Escherichia coli and specifically binds sulfate and related species (like chromate and selenate) for their transmembrane transport via the ABC transporter system encoded by the cysPUWA genes. | + | <td><img src="https://static.igem.org/mediawiki/parts/5/57/Rice_sbp.svg" style="height:30%; width:20%;"> <p> The sbp protein is found in<i>Escherichia coli</i> and specifically binds sulfate and related species (like chromate and selenate) for their transmembrane transport via the ABC transporter system encoded by the <i>cysPUWA</i> genes. BioBrick BBa_K2194001 contains only the cds for this protein. |
</p></td> | </p></td> | ||
</tr> | </tr> | ||
| Line 83: | Line 83: | ||
<td>cysPUWA Sulfate Transport System cds</td> | <td>cysPUWA Sulfate Transport System cds</td> | ||
<td>composite</td> | <td>composite</td> | ||
| − | <td><img src="https://static.igem.org/mediawiki/parts/d/d4/CysPUWA_rice.svg" style="width:70% !important; height:90%" !important; > <p>The cysPUWA genes encode the sulfate ABC transporter in Escherichia coli. This system also transports related species such as chromate. | + | <td><img src="https://static.igem.org/mediawiki/parts/d/d4/CysPUWA_rice.svg" style="width:70% !important; height:90%" !important; > <p>The <i>cysPUWA</i> genes encode the sulfate ABC membrane transporter in <i>Escherichia coli</i>. This system also transports related species such as chromate. BioBrick BBa_K2194003 contains the cds for <i>cysP</i>, <i>cysU</i>, <i>cysW</i>, and <i>cysA</i> in the context in which they appear in <i>E. coli</i> MG1655. |
</p></td> | </p></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href='http://parts.igem.org/Part:BBa_K2194004' style='color:rgb(153,117,185)'><font color='Indigo '>BBa_K2194004</font></a></td> | <td><a href='http://parts.igem.org/Part:BBa_K2194004' style='color:rgb(153,117,185)'><font color='Indigo '>BBa_K2194004</font></a></td> | ||
| − | <td> | + | <td>Sulfate Transport System w/ Negative Feedback for Membrane Stress</td> |
<td>composite</td> | <td>composite</td> | ||
| − | <td><img src="https://static.igem.org/mediawiki/parts/1/11/PgntK%2C_sbp%2C_cys.svg" style="height:100%; width:90%;" > | + | <td><img src="https://static.igem.org/mediawiki/parts/1/11/PgntK%2C_sbp%2C_cys.svg" style="height:100%; width:90%;" > <p>This transcriptional unit codes for the genetic parts necessary to bind and transport chromate, sulfate, and related species across the cell membrane. BioBrick BBa_K2194004 is a composite part composed of negative feedback membrane stress promoter <i>PgntK</i> (<a href="http://parts.igem.org/Part:BBa_K2194002">BBa_K2194002</a>), a synthetic RBS, cysPUWA proteins (<a href="http://parts.igem.org/Part:BBa_K2194003">BBa_K2194003</a>), Elowitz RBS (<a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>), sulfate binding protein sbp (<a href="http://parts.igem.org/Part:BBa_K2194001">BBa_K2194001</a>), and a synthetic terminator. </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><a href='http://parts.igem.org/Part:BBa_K2194005' style='color:rgb(153,117,185)'><font color='Indigo '>BBa_K2194005</font></a></td> | <td><a href='http://parts.igem.org/Part:BBa_K2194005' style='color:rgb(153,117,185)'><font color='Indigo '>BBa_K2194005</font></a></td> | ||
| − | <td>chrR6 | + | <td> chrR6 Chromate Reductase Enzyme with Chromate-sensitive Promoter </td> |
<td>composite</td> | <td>composite</td> | ||
| − | <td><img src="https://static.igem.org/mediawiki/parts/f/f9/Rice_Pchr%2BChrR6.svg" style="height:100%; width:40%;"> <p> | + | <td><img src="https://static.igem.org/mediawiki/parts/f/f9/Rice_Pchr%2BChrR6.svg" style="height:100%; width:40%;"> <p>This transcriptional unit codes for a chromate reductase enzyme under a chromate-sensitive promoter. BioBrick BBa_K2194005 is a composite part composed of <i>Pchr</i> chromate-sensitive promoter ("cr-promoter" feature of <a href="http://parts.igem.org/Part:BBa_K1758312">BBa_K1758312</a>), with rbs (<a href="http://parts.igem.org/Part:BBa_K1758100">BBa_K1758100</a>), and chrR6 chromate reductase (<a href="http://parts.igem.org/Part:BBa_K2194000">BBa_K2194000</a>). </p></td> |
</tr> | </tr> | ||
</table> | </table> | ||
Latest revision as of 20:19, 1 November 2017
| Part # | Name | Type | Description |
|---|---|---|---|
| BBa_K2194000 | chrR6 Chromate Reductase Enzyme | basic | 
The chrR6 protein is a mutated/enhanced version (Tyr128Asn) of the chrR enzyme originally found in Escherichia coli. It has chromate and uranyl reductase activity. BioBrick BBa_K2194000 contains only the cds for this protein.
|
| BBa_K2194002 | PgntK Negative Feedback Membrane Stress Promoter | basic |  The PgntK promoter is a native Escherichia coli negative feedback membrane stress promoter. BioBrick BBa_K2194002 contains the 200 bp upstream of the gntK gene in Escherichia coli, which has been previously experimentally shown to incorporate the full Pgntk promoter region. |
| BBa_K2194001 | Sulfate Binding Protein (sbp) cds | basic |  The sbp protein is found inEscherichia coli and specifically binds sulfate and related species (like chromate and selenate) for their transmembrane transport via the ABC transporter system encoded by the cysPUWA genes. BioBrick BBa_K2194001 contains only the cds for this protein. |
| BBa_K2194003 | cysPUWA Sulfate Transport System cds | composite |  The cysPUWA genes encode the sulfate ABC membrane transporter in Escherichia coli. This system also transports related species such as chromate. BioBrick BBa_K2194003 contains the cds for cysP, cysU, cysW, and cysA in the context in which they appear in E. coli MG1655. |
| BBa_K2194004 | Sulfate Transport System w/ Negative Feedback for Membrane Stress | composite |  This transcriptional unit codes for the genetic parts necessary to bind and transport chromate, sulfate, and related species across the cell membrane. BioBrick BBa_K2194004 is a composite part composed of negative feedback membrane stress promoter PgntK (BBa_K2194002), a synthetic RBS, cysPUWA proteins (BBa_K2194003), Elowitz RBS (BBa_B0034), sulfate binding protein sbp (BBa_K2194001), and a synthetic terminator. |
| BBa_K2194005 | chrR6 Chromate Reductase Enzyme with Chromate-sensitive Promoter | composite |  This transcriptional unit codes for a chromate reductase enzyme under a chromate-sensitive promoter. BioBrick BBa_K2194005 is a composite part composed of Pchr chromate-sensitive promoter ("cr-promoter" feature of BBa_K1758312), with rbs (BBa_K1758100), and chrR6 chromate reductase (BBa_K2194000). |
