Difference between revisions of "Team:CSMU NCHU Taiwan/Composite Part"

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    <title>Safety</title>
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<div class="column full_size judges-will-not-evaluate">
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body {
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<div class="column full_size">
 
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<h1>Composite Parts</h1>
 
  
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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  text-align: center;
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  display: none; }
  
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<h3>Best Composite Part Special Prize</h3>
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<p>New BioBrick devices can be made by combining existing BioBrick Parts. For example, Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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<h4>Note</h4>
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
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            <h1>Composite Parts</h1>
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            <p>This year, we CSMU_NCHU_Taiwan, brings you 3 composite parts.</p>
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              <br>
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              <img class="center" src="https://static.igem.org/mediawiki/2017/4/4a/Csmuxnchu_parts1.png" style="width:90%;" alt="">
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              <br>
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              <img class="center" src="https://static.igem.org/mediawiki/2017/0/0e/Csmuxnchu_part_form2.png" style="width:90%;" alt="">
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<h2>Composite Parts</h2>
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<h3>FOR ANTIDOTE</h3>
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<p><br>
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<b>T7 promoter + Thioredoxin-FGD fusion protein (<a href="http://parts.igem.org/Part:BBa_K2382005" style="color:#385e66;"target="_blank">BBa_K2382005</a>)</b><br>
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This part contains a T7 promoter and Thioredoxin-FGD fusion protein, and terminators are
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included. By ligating these two different parts as a fusion protein, it is supposed to raise the
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solubility of our protein, F420-dependent glucose-6- phosphate dehydrogenase (FGD).<br><br>
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<b>T7 promoter + Thioredoxin-MSMEG_5998 fusion protein (<a href="http://parts.igem.org/Part:BBa_K2382006"style="color:#385e66;" target="_blank">BBa_K2382006</a>)</b><br>
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This part contains a T7 promoter and Thioredoxin-MSMEG_5998 fusion protein, and terminators
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are included. By ligating these two different parts as a fusion protein, it works better than
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MSMEG_5998 alone.
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</p>
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<h3>FOR TEST STRIP</h3>
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<p><br>
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<b>Anti-aflatoxin scFv fusion protein construction DNA sequence (<a href="http://parts.igem.org/Part:BBa_K2382010"style="color:#385e66;" target="_blank">BBa_K2382010</a>)</b><br>
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By ligating the constitutive promoter (BBa_J23101), strong ribosome binding site (BBa_B0034)
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and scFv-EAAAK- RFP-Histag, we were able to detect aflatoxin by the purified protein expressing
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in E. coli. Moreover, we designed a restriction site, BamHI, between scFv and EAAAK, so future
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iGEM teams could take advantage of this composite part to fuse their scFv with RFP as indicator,
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and make their own test strip!
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</p>
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</p>
 
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Latest revision as of 20:21, 1 November 2017

HOME
Safety

Composite Parts

This year, we CSMU_NCHU_Taiwan, brings you 3 composite parts.



Composite Parts

FOR ANTIDOTE


T7 promoter + Thioredoxin-FGD fusion protein (BBa_K2382005)
This part contains a T7 promoter and Thioredoxin-FGD fusion protein, and terminators are included. By ligating these two different parts as a fusion protein, it is supposed to raise the solubility of our protein, F420-dependent glucose-6- phosphate dehydrogenase (FGD).

T7 promoter + Thioredoxin-MSMEG_5998 fusion protein (BBa_K2382006)
This part contains a T7 promoter and Thioredoxin-MSMEG_5998 fusion protein, and terminators are included. By ligating these two different parts as a fusion protein, it works better than MSMEG_5998 alone.

FOR TEST STRIP


Anti-aflatoxin scFv fusion protein construction DNA sequence (BBa_K2382010)
By ligating the constitutive promoter (BBa_J23101), strong ribosome binding site (BBa_B0034) and scFv-EAAAK- RFP-Histag, we were able to detect aflatoxin by the purified protein expressing in E. coli. Moreover, we designed a restriction site, BamHI, between scFv and EAAAK, so future iGEM teams could take advantage of this composite part to fuse their scFv with RFP as indicator, and make their own test strip!