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<h2><b>Marketing Research</b></h2> | <h2><b>Marketing Research</b></h2> | ||
<p style="font-family: roboto;font-size:115%;"> | <p style="font-family: roboto;font-size:115%;"> | ||
− | + | Example of current influenza point-of-care: | |
<br><br> | <br><br> | ||
BD Veritor™ system<sup>1</sup> <br> | BD Veritor™ system<sup>1</sup> <br> | ||
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The advantage of our design: <br> <ul> | The advantage of our design: <br> <ul> | ||
<li>Only heater/heat plate is needed<br> | <li>Only heater/heat plate is needed<br> | ||
− | <li>Even if no heater is available, incubated at room temperature for | + | <li>Even if no heater is available, incubated at room temperature for more than one hour can still show the result (stated in |
+ | <a href="https://www.promega.com/products/protein-expression/protein-expression-kits-and-reagents/s30-t7-high-yield-protein-expression-system/?catNum=L1110">manufacturer's protocol</a>)<br> | ||
<li>Result is reported by colour changes, no special demonstrator is required <br> | <li>Result is reported by colour changes, no special demonstrator is required <br> | ||
<li>Throat swab is possible, increasing choice of sampling </ul> | <li>Throat swab is possible, increasing choice of sampling </ul> | ||
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<h4><u>3. Time</u></h4><br> | <h4><u>3. Time</u></h4><br> | ||
<p style="font-family: roboto;font-size:115%;"> | <p style="font-family: roboto;font-size:115%;"> | ||
− | Ordinary and typical diagnosis procedure always spend an entire day long. After the conversations with two medical experts, it is known that shorter the diagnosis time, quicker for patients to undergo medical treatment. Thus, we aim to shorter the detection time. Also, we have considered that heater/ heat plate is not common, or even unavailable in the suburb, remote districts or some developing countries. The reporter chromoprotein can still be expressed under room temperature, however, its disadvantage is that users need to wait | + | Ordinary and typical diagnosis procedure always spend an entire day long. After the conversations with two medical experts, it is known that shorter the diagnosis time, quicker for patients to undergo medical treatment. Thus, we aim to shorter the detection time. Also, we have considered that heater/ heat plate is not common, or even unavailable in the suburb, remote districts or some developing countries. The reporter chromoprotein can still be expressed under room temperature, however, its disadvantage is that users need to wait more than an hour. But still, it provides convenience to people living in regions with limited resources. |
</p> | </p> | ||
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<br><br> | <br><br> | ||
<b>b) Procedure</b><br> | <b>b) Procedure</b><br> | ||
− | At the beginning, saliva | + | At the beginning, we proposed that saliva can be the source of sampling due to its easy accessibility. After interviewing with Prof Chan, he indicated that nasopharyngeal aspirate is the most common sampling source in diagnosis, but the assistance of nurse/ medical professional is needed. Besides, he suggested that throat swab is the possible sampling way in the condition that our kit is sensitive and specific enough. |
<br><br> | <br><br> | ||
− | Prior to detection, water is required to add to reactivate the cell-free system as the cell-free system is inactivated as freeze-dried. For the sake of easy operation, another plate with pre-inserted water is prepared. During detection, sample will be load into the plate with water first to stabilize the protein. After that, standardized amount is extracted (50ul is set) followed by the sample, what the detection kit needs is only the heater/heat plate which is able to heat up to 37 degree Celsius (°C), and wait for 3 to 4 hours, or alternatively, if unfortunately, no heater/heat plate is available, the chromoprotein can be expressed at the 25 Celsius (°C), but need to wait for | + | Prior to detection, water is required to add to reactivate the cell-free system as the cell-free system is inactivated as freeze-dried. For the sake of easy operation, another plate with pre-inserted water is prepared. During detection, sample will be load into the plate with water first to stabilize the protein. After that, standardized amount is extracted (50ul is set) followed by the sample, what the detection kit needs is only the heater/heat plate which is able to heat up to 37 degree Celsius (°C), and wait for 3 to 4 hours, or alternatively, if unfortunately, no heater/heat plate is available, the chromoprotein can be expressed at the 25 Celsius (°C), but need to wait for more than an hour. At the end, no special equipment/machine is needed for result identification, instead, the result is clarified in a color change attributed to the presence of chromoprotein. Avian influenza pose more threats on human, for instance, H5N1, H7N9 rendered negative and far-reaching influences on both economy and public health system in Hong Kong before. Thus, specially, we will include the detections of H5N1, H7N9 inside the kit, just as what medical experts and health department had said, rapid confirmation of whether suffering influenza, especially avian influenza helps quicker start of medical treatment. |
</p> | </p> | ||
− | Controls | + | Controls will also be tested at the same time so as to prove the functionality of our design, the toehold switch. Three controls will be performed: one positive control that verify the working of cell free system; another positive control to ensure influenza A is tested; and negative control that contain no DNA. |
</p> | </p> | ||
<h4><u>2. Detector</u></h4><br> | <h4><u>2. Detector</u></h4><br> | ||
<p style="font-family: roboto;font-size:115%;"> | <p style="font-family: roboto;font-size:115%;"> | ||
− | During use, samples are loaded into the wells of our bio-design ( | + | During use, samples are loaded into the wells of our bio-design (See <b>"Design"</b> below, each kit will only contain one of them), and the results are shown in the form of colour changes <br> |
+ | |||
</p> | </p> | ||
<h4><u>3.Cotton wool</u></h4><br> | <h4><u>3.Cotton wool</u></h4><br> | ||
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<br><br> | <br><br> | ||
<b>Design 3 (Tablets package)</b><br> | <b>Design 3 (Tablets package)</b><br> | ||
− | In response to the concern raised by Prof Chan, we designed another design and the appearance is tablet package alike. As the bottom is curve-like shape, even if the freeze-dried cell-free system melt and spread around, or cannot condense totally at the bottom, the curve-shape can help settle down the cell-free solution. During the use, tear off the firm firstly, and add a certain amount of water by dropper. After hydration, similar to previous design, add the sample by cotton wool. The procedures afterward (eg the verification) are equivalent to previous design. | + | In response to the concern raised by Prof Chan, we designed another design and the appearance is tablet package alike. The UCCKE team helped us to 3D-printed the model (see our <a href="https://2017.igem.org/Team:Hong_Kong-CUHK/Collaborations"> collaboration page</a>). As the bottom is curve-like shape, even if the freeze-dried cell-free system melt and spread around, or cannot condense totally at the bottom, the curve-shape can help settle down the cell-free solution. During the use, tear off the firm firstly, and add a certain amount of water by dropper. After hydration, similar to previous design, add the sample by cotton wool. The procedures afterward (eg the verification) are equivalent to previous design. |
<br><br> | <br><br> | ||
− | Apart from smaller size when compared to the previous design, fewer materials are used, which is more environmental-friendly. For the medical track, as mentioned above in procedure section, there are 7 wells (with freeze-dried cell-free system) in one plate and every patient will only consume 1 plate. | + | Apart from smaller size when compared to the previous design, fewer materials are used, which is more environmental-friendly. For the medical track, as mentioned above in procedure section, there are 7 wells (with freeze-dried cell-free system) in one plate and every patient will only consume 1 plate. |
<br><br> | <br><br> | ||
For the sake of convenience, especially on-site mass-screening detection carried by immigration or agriculture department, a larger plate with more wells is prepared, allowing mass detection at the same time. | For the sake of convenience, especially on-site mass-screening detection carried by immigration or agriculture department, a larger plate with more wells is prepared, allowing mass detection at the same time. | ||
− | + | ||
+ | |||
<div> | <div> | ||
<div id="myCarousel2" class="carousel slide" data-ride="carousel"> | <div id="myCarousel2" class="carousel slide" data-ride="carousel"> | ||
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<div class="item"> | <div class="item"> | ||
− | <center><img src="https://static.igem.org/mediawiki/2017/ | + | <center><img src="https://static.igem.org/mediawiki/2017/2/2b/CUHK_3dmodel.jpeg" height="300"></center> |
</div> | </div> | ||
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</div> | </div> | ||
− | </div> | + | </div> </div> |
<p style="font-family: roboto;font-size:115%;"> | <p style="font-family: roboto;font-size:115%;"> |
Latest revision as of 20:46, 1 November 2017