Difference between revisions of "Team:ETH Zurich/Parts"

 
(33 intermediate revisions by 3 users not shown)
Line 10: Line 10:
 
<h1 class="headline">Parts</h1>
 
<h1 class="headline">Parts</h1>
  
−
<figure class="biobrick">
+
 
 +
<figure class="top-fig-single">
 
     <img src="https://static.igem.org/mediawiki/2017/7/7e/T--ETH_Zurich--basicparts.png" alt="FIXME">
 
     <img src="https://static.igem.org/mediawiki/2017/7/7e/T--ETH_Zurich--basicparts.png" alt="FIXME">
 
</figure>
 
</figure>
  
 
<section class="overview">
 
<section class="overview">
−
    <h1> Overview </h1>
+
 
−
     <p>We submitted in total 14 new BioBricks to the iGEM registry! You can find all parts and their design below.</p>
+
<h1>Overview</h1>
 +
 
 +
     <p>We submitted in total 14 new BioBricks to the iGEM registry! You can find all of them below in our list of parts. Curious to learn more? Visit <a href="https://2017.igem.org/Team:ETH_Zurich/Basic_Part">Basic Parts</a> and <a href="https://2017.igem.org/Team:ETH_Zurich/Composite_Part">Composite Parts</a> for more details.</p>
 
</section>
 
</section>
  
 
<section>
 
<section>
−
     <h1>Basic Parts</h1>
+
     <h1>List of Parts</h1>
  
 
     <table width="100%">
 
     <table width="100%">
Line 49: Line 52:
 
         <tr>
 
         <tr>
 
             <!--<td>Image</td>-->
 
             <!--<td>Image</td>-->
−
             <td>p<sub>TlpA</sub></td>
+
             <td>P<sub>TlpA</sub></td>
 
             <td>Temperature-responsive promoter optimized for slight activation above 37°C and full activation at 45 °C</td>
 
             <td>Temperature-responsive promoter optimized for slight activation above 37°C and full activation at 45 °C</td>
 
             <td><a href="http://parts.igem.org/Part:BBa_K2500003">BBa_K2500003</a></td>
 
             <td><a href="http://parts.igem.org/Part:BBa_K2500003">BBa_K2500003</a></td>
Line 77: Line 80:
 
             <td><a href="http://parts.igem.org/Part:BBa_K2500007">BBa_K2500007</a></td>
 
             <td><a href="http://parts.igem.org/Part:BBa_K2500007">BBa_K2500007</a></td>
 
         </tr>
 
         </tr>
−
 
+
<tr>
 +
            <!--<td>Image</td>-->
 +
            <td>P<sub>Const</sub>RBS<sub>eng</sub>_TlpA</td>
 +
            <td><strong>Best Composite Part:</strong> Constitutively expressed temperature-dependent transcriptional repressor of p<sub>Tlpa</sub> with engineered RBS</td>
 +
            <td><a href="http://parts.igem.org/Part:BBa_K2500008">BBa_K2500008</a></td>
 +
        </tr>
 
         <tr>
 
         <tr>
 
             <td>RBS<sub>eng</sub>_ProteinE</td>
 
             <td>RBS<sub>eng</sub>_ProteinE</td>
Line 91: Line 99:
 
         <tr>
 
         <tr>
 
             <!--<td>Image</td>-->
 
             <!--<td>Image</td>-->
−
             <td><strong>Best Basic Part:</strong><br>AND Gate B</td>
+
             <td>AND Gate B</td>
−
             <td>Synthetic promoter responsive to LldR and luxR </td>
+
             <td><strong>Best Basic Part:</strong> Synthetic promoter responsive to LldR and luxR </td>
 
             <td><a href="http://parts.igem.org/Part:BBa_K2500011">BBa_K2500011</a></td>
 
             <td><a href="http://parts.igem.org/Part:BBa_K2500011">BBa_K2500011</a></td>
 
         </tr>
 
         </tr>
Line 102: Line 110:
 
         </tr>
 
         </tr>
 
       <tr>
 
       <tr>
−
             <td>p<sub>Const</sub>_RBS_LldP/<br>LldR_p<sub>Const</sub>_RBS_LuxR</td>
+
             <td>P<sub>Const</sub>_RBS_LldP/<br>LldR_P<sub>Const</sub>_RBS_LuxR</td>
 
             <td>Expression cassette consisting of LuxR and LldP/LldR</td>
 
             <td>Expression cassette consisting of LuxR and LldP/LldR</td>
 
             <td><a href="http://parts.igem.org/Part:BBa_K2500013">BBa_K2500013</a></td>
 
             <td><a href="http://parts.igem.org/Part:BBa_K2500013">BBa_K2500013</a></td>
Line 109: Line 117:
 
</section>
 
</section>
  
−
<section>
 
−
    <h1>Composite Parts</h1>
 
  
−
    <table width="100%">
 
−
        <tr>
 
−
            <th>Part</th>
 
−
            <th>Description</th>
 
−
            <th>BioBrick</th>
 
−
        </tr>
 
−
<tr>
 
−
            <!--<td>Image</td>-->
 
−
            <td>p<sub>Const</sub>RBS<sub>eng</sub>_TlpA</td>
 
−
            <td>Constitutively expressed temperature-dependent transcriptional repressor of p<sub>Tlpa</sub> with engineered RBS</td>
 
−
            <td><a href="http://parts.igem.org/Part:BBa_K2500008">BBa_K2500008</a></td>
 
−
        </tr>
 
  
−
    </table>
 
−
</section>
 
−
 
−
<section class="design">
 
−
    <h1>Design</h1>
 
−
 
−
    <div class="multi-summary">
 
−
 
−
<details>
 
−
<summary>BBa_K2500000: Bacterioferritin</summary>
 
−
<figure>
 
−
<img src="#" alt="FIXME">
 
−
</figure>
 
−
<p>Description.</p>
 
−
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500000">BBa_K2500000</a></p>
 
−
</details>
 
−
 
−
<details>
 
−
<summary>BBa_K2500001: Azurin</summary>
 
−
<figure>
 
−
<img src="#" alt="FIXME">
 
−
</figure>
 
−
<p>Description.</p>
 
−
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500001">BBa_K2500001</a></p>
 
−
</details>
 
−
 
−
<details>
 
−
<summary>BBa_K2500002: p28</summary>
 
−
<figure>
 
−
<img src="#" alt="FIXME">
 
−
</figure>
 
−
<p>Description.</p>
 
−
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500002">BBa_K2500002</a></p>
 
−
</details>
 
−
 
−
<details>
 
−
<summary>BBa_K2500003: p<sub>TlpA</sub></summary>
 
−
<figure>
 
−
<img src="#" alt="FIXME">
 
−
</figure>
 
−
<p>Description.</p>
 
−
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500003">BBa_K2500003</a></p>
 
−
</details>
 
−
 
−
<details>
 
−
<summary>BBa_K2500004: TlpA</summary>
 
−
<figure>
 
−
<img src="#" alt="FIXME">
 
−
</figure>
 
−
<p>Description.</p>
 
−
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500004">BBa_K2500004</a></p>
 
−
</details>
 
−
 
−
<details>
 
−
<summary>BBa_K2500005: RBS_TlpA</summary>
 
−
<figure>
 
−
<img src="#" alt="FIXME">
 
−
</figure>
 
−
<p>Description.</p>
 
−
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500005">BBa_K2500005</a></p>
 
−
</details>
 
−
 
−
<details>
 
−
<summary>BBa_K2500006: Protein E</summary>
 
−
<figure>
 
−
<img src="#" alt="FIXME">
 
−
</figure>
 
−
<p>Description.</p>
 
−
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500006">BBa_K2500006</a></p>
 
−
</details>
 
−
 
−
<details>
 
−
<summary>BBa_K2500007: RBS<sub>eng</sub>_TlpA</summary>
 
−
<figure>
 
−
<img src="#" alt="FIXME">
 
−
</figure>
 
−
<p>Description.</p>
 
−
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500007">BBa_K2500007</a></p>
 
−
</details>
 
−
 
−
       
 
−
<details>
 
−
<summary>BBa_K2500008: p<sub>Const</sub>_RBS_LldP/LldR_p<sub>Const</sub>_RBS_LuxR</summary>
 
−
<figure>
 
−
<img src="#" alt="FIXME">
 
−
</figure>
 
−
<p>Description.</p>
 
−
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500008">BBa_K2500008</a></p>
 
−
</details>
 
−
 
−
<details>
 
−
<summary>BBa_K2500009: RBS<sub>eng</sub>_ProteinE</summary>
 
−
<figure>
 
−
<img src="#" alt="FIXME">
 
−
</figure>
 
−
<p>Description.</p>
 
−
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500009">BBa_K2500009</a></p>
 
−
</details>
 
−
 
−
 
−
<details>
 
−
<summary>BBa_K2500010: AND gate A</summary>
 
−
<figure class="A">
 
−
<img src="https://static.igem.org/mediawiki/2017/7/72/T--ETH_Zurich--ANDgateA.png" alt="FIXME">
 
−
</figure>
 
−
<p>In the absence of high concentrations of L-lactate, LldR inhibitor proteins bind to the binding sites O1 and O2 surrounding the pLux promoter leading to the formation of a DNA loop. The pLux promoter is sequestered and inaccessible for transcription. In design A, the distances between the intercalated promoter and the binding sites were adapted from <a href="http://parts.igem.org/Part:BBa_K1847007">BBa_K1847007</a>.</p>
 
−
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500010">BBa_K2500010</a></p>
 
−
</details>
 
−
 
−
 
−
<details>
 
−
<summary>BBa_K2500011: AND Gate B</summary>
 
−
<figure class="B">
 
−
<img src="https://static.igem.org/mediawiki/2017/6/6a/T--ETH_Zurich--ANDgateB.png" alt="FIXME">
 
−
</figure>
 
−
<p>In design B, each binding site was duplicated in order to achieve a potential zipper mechanism and stronger inhibition due to binding more LldR inhibitor proteins.</p>
 
−
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500011">BBa_K2500011</a></p>
 
−
</details>
 
−
 
−
<details>
 
−
<summary>BBa_K2500012: AND Gate C</summary>
 
−
<figure class="C">
 
−
<img src="https://static.igem.org/mediawiki/2017/c/c2/T--ETH_Zurich--ANDgateC.png" alt="FIXME">
 
−
</figure>
 
−
<p>In design C, an artificial spacer was embedded between the pLux promoter and the O2 binding site in order to influence the looping dynamics.</p>
 
−
<p> Registry: <a href="http://parts.igem.org/Part:BBa_K2500012">BBa_K2500012</a></p>
 
−
</details>
 
−
</div>
 
−
</section>
 
−
 
−
<!--
 
−
<section>
 
−
    <h1>Inspiration</h1>
 
−
    <p>We have a created a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started. You can also take a look at how other teams have documented their parts in their wiki:</p>
 
−
    <ul>
 
−
        <li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
−
        <li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
−
        <li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
−
    </ul>
 
−
</section>
 
−
<section>
 
−
    <h1>Part Table </h1>
 
−
    <p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry.</p>
 
−
    <groupparts>iGEM17 ETH_Zurich</groupparts>
 
−
</section>
 
−
-->
 
 
</main>
 
</main>
 
</html>
 
</html>
 
{{ETH_Zurich/Footer_N}}
 
{{ETH_Zurich/Footer_N}}

Latest revision as of 21:29, 1 November 2017

Parts

FIXME

Overview

We submitted in total 14 new BioBricks to the iGEM registry! You can find all of them below in our list of parts. Curious to learn more? Visit Basic Parts and Composite Parts for more details.

List of Parts

Part Description BioBrick
Bacterioferritin Heme-deletion mutant of bacterial iron storage protein functioning as an MRI contrast agent BBa_K2500000
Azurin Redox protein originating from P. aeruginosa with anti-cancer activity BBa_K2500001
p28 Effector domain of azurin BBa_K2500002
PTlpA Temperature-responsive promoter optimized for slight activation above 37°C and full activation at 45 °C BBa_K2500003
TlpA Temperature-dependent transcriptional repressor of pTlpa BBa_K2500004
RBS_TlpA Temperature-dependent transcriptional repressor of pTlpa and synthetic RBS BBa_K2500005
Protein E Bacteria-lysing protein encoded by phage Phi X 17 BBa_K2500006
RBSeng_TlpA Temperature-dependent transcriptional repressor of pTlpa with engineered RBS BBa_K2500007
PConstRBSeng_TlpA Best Composite Part: Constitutively expressed temperature-dependent transcriptional repressor of pTlpa with engineered RBS BBa_K2500008
RBSeng_ProteinE Bacteria-lysing protein encoded by phage Phi X 174 with engineered RBS BBa_K2500009
AND Gate A Synthetic promoter responsive to LldR and luxR BBa_K2500010
AND Gate B Best Basic Part: Synthetic promoter responsive to LldR and luxR BBa_K2500011
AND Gate C Synthetic promoter responsive to LldR and luxR BBa_K2500012
PConst_RBS_LldP/
LldR_PConst_RBS_LuxR		 Expression cassette consisting of LuxR and LldP/LldR BBa_K2500013