Difference between revisions of "Team:ICT-Mumbai/Safety"

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{{ICT-Mumbai}}
 
 
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<title>ICT-Mumbai 2017</title>
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<h1> Safety </h1>
 
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<p>Please visit <a href="https://2017.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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body,h1,h2,h3,h4,h5,h6 {font-weight: 'bold'; font-family: 'Lato', sans-serif;}
  
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body, html {
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    background-image: url("https://static.igem.org/mediawiki/2017/1/16/ICT-Mumbai_safety_bgimage.png");
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      <i class="fa fa-bars"></i>
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    </a>
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  <a href="https://2017.igem.org/Team:ICT-Mumbai" class="w3-bar-item w3-button w3-hide-small"><i class="fa fa-home" aria-hidden="true"></i>
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Home</a>
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    Project</button></a>
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        <a href="https://2017.igem.org/Team:ICT-Mumbai/Background" class="w3-bar-item w3-button">Background</a>
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        <a href="https://2017.igem.org/Team:ICT-Mumbai/Description" class="w3-bar-item w3-button">Description</a>
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        <a href="https://2017.igem.org/Team:ICT-Mumbai/Design" class="w3-bar-item w3-button">Design</a>
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        <a href="https://2017.igem.org/Team:ICT-Mumbai/Notebook"class="w3-bar-item w3-button">Notebook</a>
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        <a href="https://2017.igem.org/Team:ICT-Mumbai/Protocols"class="w3-bar-item w3-button">Protocols</a>
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        <a href="https://2017.igem.org/Team:ICT-Mumbai/InterLab"class="w3-bar-item w3-button">InterLab</a>
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        <a href="https://2017.igem.org/Team:ICT-Mumbai/Results"class="w3-bar-item w3-button">Results</a>
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</div>
 
</div>
 
</div>
  
  
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<div class="column full_size">
 
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<h5>Safe Project Design</h5>
 
  
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
  
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    <div class="w3-dropdown-hover">
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<li>Choosing a non-pathogenic chassis</li>
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<button class="w3-button" class="w3-bar-item w3-button w3-hide-small"><i class="fa fa-users" aria-hidden="true"></i>Team</button></a>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<div class="w3-dropdown-content w3-bar-block w3-animate-opacity">
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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        <a href="https://2017.igem.org/Team:ICT-Mumbai/Team"class="w3-bar-item w3-button">Team</a>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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        <a href="https://2017.igem.org/Team:ICT-Mumbai/Collaborations" class="w3-bar-item w3-button">Collaborations</a>
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</ul>
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</div>
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</div>
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    <div class="w3-dropdown-hover">
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<a href="https://2017.igem.org/Team:ICT-Mumbai/Parts" button class="w3-button" class="w3-bar-item w3-button w3-hide-small"><i class="fa fa-cogs" aria-hidden="true"></i> Part</a>
  
 
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</div>
  
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<div class="column half_size">
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<a href="https://2017.igem.org/Team:ICT-Mumbai/Safety" class="w3-bar-item w3-button w3-hide-small"><i class="fa fa-flag" aria-hidden="true"></i>
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<h5>Safe Lab Work</h5>
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Safety</a>
  
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<a href="https://2017.igem.org/Team:ICT-Mumbai/HP/Gold_Integrated" class="w3-bar-item w3-button w3-hide-small"><i class="fa fa-handshake-o" aria-hidden="true"></i>
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Human Practices</a>
  
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<a href="https://2017.igem.org/Team:ICT-Mumbai/Attributions" class="w3-bar-item w3-button w3-hide-small"><i class="fa fa-user-o" aria-hidden="true"></i>Attributions</a>
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    <a href="#about" class="w3-bar-item w3-button" onclick="toggleFunction()">ABOUT</a>
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    <a href="#portfolio" class="w3-bar-item w3-button" onclick="toggleFunction()">PORTFOLIO</a>
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    <a href="#contact" class="w3-bar-item w3-button" onclick="toggleFunction()">CONTACT</a>
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    <a href="#" class="w3-bar-item w3-button">SEARCH</a>
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<h5>Safe Shipment</h5>
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<div class="bgimg-1 w3-display-container w3-opacity-min" id="home">
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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<!-- Container (About Section)-->
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  <!--<h3 class="w3-center" style="font-weight: bold">Team ICT-Mumbai 2017</h3>
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<br>-->
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<!--<h4 class="w3-center">ABSTRACT</h4>-->
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  <h5 class="w3-center" style="color:#000080">Our host organism</h5>
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<p style="height:70 px; font-family:'Lato'; font-size:20px; colour:lightgrey;">The host organism of our work is the ‘lab pet’ / ‘industrial workhorse’ <i>Escherichia coli</i>. We have used
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different strains of <i>E. coli</i> for our work – DH5α, BW25113, BL21(DE3) – all of which are categorised
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under Biosafety Level I (BSL I) and safe to use and handle.</p>
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<div class="w3-content w3-container w3-padding-64" id="about">
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  <h5 class="w3-center" style="color:#000080">Safe practices in our laboratory</h5>
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<p style="height:70 px; font-family:'Lato'; font-size:20px; colour:lightgrey;">We carried out our work in the DBT-ICT Centre for Energy Biosciences, which houses state-of- the-art facilities for carrying out microbiology and synthetic biology workflows. We handled bacteria in biosafety laminar flow units, and discarded all waste in plastic containers that were autoclaved before disposal. We put on latex gloves and used spatulas to handle ethidium bromide-containing agarose gels. Used gels were put in labelled bags that were handed over to a contractor for disposal by incineration at a municipal facility.</p>
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    <img src="https://static.igem.org/mediawiki/2017/e/e4/Autoclave_ict.jpeg" alt="Trolltunga Norway" width="600" height="600">
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  <div class="desc">Autoclave facility</div>
 
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    <img src="https://static.igem.org/mediawiki/2017/1/14/Min1_1509506194_59.182.136.30.jpg" alt="Forest" width="600" height="600">
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  <div class="desc">Laminar air flow facility</div>
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<div class="gallery">
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  <div class="desc">Bio-medical waste disposal bags</div>
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<div class="w3-content w3-container w3-padding-64" id="about">
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  <h5 class="w3-center" style="color:#000080">Ensuring safety in our Institute</h5>
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<p style="height:70 px; font-family:'Lato'; font-size:20px; colour:lightgrey;">The Institutional Biosafety Committee of our Institute is responsible for ensuring that safe practices are followed in all laboratories in the Institute. The Committee meets twice a year to review and recommend safety measures. Our team primary PI, Prof. Arvind Lali, is the Member Secretary of the Committee.</p>
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<div class="w3-content w3-container w3-padding-64" id="about">
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  <h5 class="w3-center" style="color:#000080">Safety regulations in our country</h5>
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<p style="height:70 px; font-family:'Lato'; font-size:20px; colour:lightgrey;">The Department of Biotechnology, Government of India has laid down biosafety guidelines that all researchers in the country have to adhere to. The relevant biosafety rules and regulations can be accessed at <a href="http://dbtbiosafety.nic.in/"><mark>http://dbtbiosafety.nic.in/</mark></a></p>
  
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Latest revision as of 21:52, 1 November 2017

ICT-Mumbai 2017

Our host organism

The host organism of our work is the ‘lab pet’ / ‘industrial workhorse’ Escherichia coli. We have used different strains of E. coli for our work – DH5α, BW25113, BL21(DE3) – all of which are categorised under Biosafety Level I (BSL I) and safe to use and handle.

Safe practices in our laboratory

We carried out our work in the DBT-ICT Centre for Energy Biosciences, which houses state-of- the-art facilities for carrying out microbiology and synthetic biology workflows. We handled bacteria in biosafety laminar flow units, and discarded all waste in plastic containers that were autoclaved before disposal. We put on latex gloves and used spatulas to handle ethidium bromide-containing agarose gels. Used gels were put in labelled bags that were handed over to a contractor for disposal by incineration at a municipal facility.

Trolltunga Norway
Autoclave facility
Forest
Laminar air flow facility
Forest
Bio-medical waste disposal bags
Ensuring safety in our Institute

The Institutional Biosafety Committee of our Institute is responsible for ensuring that safe practices are followed in all laboratories in the Institute. The Committee meets twice a year to review and recommend safety measures. Our team primary PI, Prof. Arvind Lali, is the Member Secretary of the Committee.

Safety regulations in our country

The Department of Biotechnology, Government of India has laid down biosafety guidelines that all researchers in the country have to adhere to. The relevant biosafety rules and regulations can be accessed at http://dbtbiosafety.nic.in/