Difference between revisions of "Team:ICT-Mumbai/Safety"

 
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   <a href="https://2017.igem.org/Team:ICT-Mumbai" class="w3-bar-item w3-button w3-hide-small"><i class="fa fa-home" aria-hidden="true"></i>
 
Home</a>
 
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  <p style="font-size:20px; font-family:'Lato', sans-serif; colour:lightgrey;">Ammonia released due to hydrolysis of urine is a major reason for the stench in public toilets. Apart from being a put-off, ammonia is also hazardous. Current approaches to tackle this problem include flushing water, using microbes (for example, BioBlocks®) that break down urea in urine to prevent its hydrolysis, and using air fresheners. The first approach requires using copious amounts of water, which is not abundantly available in many parts of the world, while the second is not cost-effective. Finally, spraying air fresheners or using deodorizers is not a solution, as it does not get rid of ammonia. We propose to engineer <i>Escherichia coli</i> to assimilate ammonia and convert it into indigoidine, a blue coloured compound. Ammonia from air can be dissolved in an aqueous medium and can act as a nitrogen source for the engineered cells that can be housed in a cassette. The synthesized indigoidine will indicate when the cassette has to be replaced.</p>
 
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<p style="height:70 px; font-family:'Lato'; font-size:20px; colour:lightgrey;">The host organism of our work is the ‘lab pet’ / ‘industrial workhorse’ <i>Escherichia coli</i>. We have used
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different strains of <i>E. coli</i> for our work – DH5α, BW25113, BL21(DE3) – all of which are categorised
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<source src="https://static.igem.org/mediawiki/2017/7/71/Dyeodorant_description_video.mp4" type="video/mp4"/></center></section>
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under Biosafety Level I (BSL I) and safe to use and handle.</p>
  
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<p style="height:70 px; font-family:'Lato'; font-size:20px; colour:lightgrey;">We carried out our work in the DBT-ICT Centre for Energy Biosciences, which houses state-of- the-art facilities for carrying out microbiology and synthetic biology workflows. We handled bacteria in biosafety laminar flow units, and discarded all waste in plastic containers that were autoclaved before disposal. We put on latex gloves and used spatulas to handle ethidium bromide-containing agarose gels. Used gels were put in labelled bags that were handed over to a contractor for disposal by incineration at a municipal facility.</p>
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<p style="height:70 px; font-family:'Lato'; font-size:20px; colour:lightgrey;">The Institutional Biosafety Committee of our Institute is responsible for ensuring that safe practices are followed in all laboratories in the Institute. The Committee meets twice a year to review and recommend safety measures. Our team primary PI, Prof. Arvind Lali, is the Member Secretary of the Committee.</p>
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  <h5 class="w3-center" style="color:#000080">Safety regulations in our country</h5>
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<p style="height:70 px; font-family:'Lato'; font-size:20px; colour:lightgrey;">The Department of Biotechnology, Government of India has laid down biosafety guidelines that all researchers in the country have to adhere to. The relevant biosafety rules and regulations can be accessed at <a href="http://dbtbiosafety.nic.in/"><mark>http://dbtbiosafety.nic.in/</mark></a></p>
  
 
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Latest revision as of 21:52, 1 November 2017

ICT-Mumbai 2017

Our host organism

The host organism of our work is the ‘lab pet’ / ‘industrial workhorse’ Escherichia coli. We have used different strains of E. coli for our work – DH5α, BW25113, BL21(DE3) – all of which are categorised under Biosafety Level I (BSL I) and safe to use and handle.

Safe practices in our laboratory

We carried out our work in the DBT-ICT Centre for Energy Biosciences, which houses state-of- the-art facilities for carrying out microbiology and synthetic biology workflows. We handled bacteria in biosafety laminar flow units, and discarded all waste in plastic containers that were autoclaved before disposal. We put on latex gloves and used spatulas to handle ethidium bromide-containing agarose gels. Used gels were put in labelled bags that were handed over to a contractor for disposal by incineration at a municipal facility.

Trolltunga Norway
Autoclave facility
Forest
Laminar air flow facility
Forest
Bio-medical waste disposal bags
Ensuring safety in our Institute

The Institutional Biosafety Committee of our Institute is responsible for ensuring that safe practices are followed in all laboratories in the Institute. The Committee meets twice a year to review and recommend safety measures. Our team primary PI, Prof. Arvind Lali, is the Member Secretary of the Committee.

Safety regulations in our country

The Department of Biotechnology, Government of India has laid down biosafety guidelines that all researchers in the country have to adhere to. The relevant biosafety rules and regulations can be accessed at http://dbtbiosafety.nic.in/