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Revision as of 22:07, 1 November 2017
Feel free to contact us, if you want to know more about our characterization: igem.toulouse@gmail.com
BBa_J04450 was tested in the Vibrio harveyi background. The biobrick was cloned in a broad host range plasmid (pBBR1MCS-4) and conjugated into Vibrio harveyi to demonstrate the production of RFP in this chassis.
To learn more: see <partinfo>BBa_J04450</partinfo> and our results.
In this part encoding sequence of D-NY15 was placed under the yeast constitutive promoter pGAP. This part was characterized by RT-qPCR.
To learn more: see <partinfo>BBa_K431009</partinfo> and our results.
The α-factor sequence contains a kozak region and a signal sequence to secrete the produced peptides. The functionality of the signal factor was investigated by demonstrating that AMP are present in the supernatant using a toxicity assay.
To learn more: see <partinfo>BBa_K180001</partinfo> and our results.
This part includes Odr-10 receptor under the control of pGAP, a constitutive yeast promoter. When diacetyl binds to Odr-10 a cascade of activation of Ste proteins (endogenous to P. pastoris ) will lead to the binding of Ste12 on pFUS1 promoter, and the expression of RFP should be activated.
To learn more: see <partinfo>BBa_K1072010</partinfo>, <partinfo>BBa_K1072023</partinfo> and our results.
Contribution
Here can you find the existing Parts we have contributed to characterized
<partinfo>BBa_J04450</partinfo>: RFP coding device
<partinfo>BBa_K431009</partinfo>: glyceraldehyde 3-phosphate dehydrogenase promoter (pGAP)
<partinfo>BBa_K1800001</partinfo>: alpha factor secretion signal
Pichia pastoris complete module with reporter gene : Odr-10 diacetyl receptor (<partinfo>BBa_K1072010</partinfo>), pFUS1 (<partinfo>BBa_K1072023</partinfo>)
This system allowed to produce RFP in response to diacetyl.
This construction was cloned in pPICZalpha yeast vector.
Realisations pages
Overview
Notebook
Clonings
Results
Contribution
Protocols
Safety