Latest revision as of 22:20, 1 November 2017

Notebook

Here is a summary of what we did for our project, week by week. You can go on our Human Practices labbook page to know more about how each event affected us to take a decision about the future of our project.

Brainstorming meeting

Team day

Meetup with other iGEM teams

Public Engagement event

Integrated Human Practices / Entrepreneurship major step

Experiments

January

1	2	3	4	5	6	7
8	9	10	11	12	13	14
15	16	17	18	19	20	21
22	23	24	25	26	27	28
29	30	31	1	2	3	4

February

29	30	31	1	2	3	4
5	6	7	8	9	10	11
12	13	14	15	16	17	18
19	20	21	22	23	24	25
26	27	28	1	2	3	4

March

26	27	28	1	2	3	4
5	6	7	8	9	10	11
12	13	14	15	16	17	18
19	20	21	22	23	24	25
26	27	28	29	30	31	1

April

26	27	28	29	30	31	1
2	3	4	5	6	7	8
9	10	11	12	13	14	15
16	17	18	19	20	21	22
23	24	25	26	27	28	29
30	1	2	3	4	5	6

May

30	1	2	3	4	5	6
7	8	9	10	11	12	13
14	15	16	17	18	19	20
21	22	23	24	25	26	27
28	29	30	31	1	2	3

June

28	29	30	31	1	2	3
4	5	6	7	8	9	10
11	12	13	14	15	16	17
18	19	20	21	22	23	24
25	26	27	28	29	30	1

July

25	26	27	28	29	30	1
2	3	4	5	6	7	8
9	10	11	12	13	14	15
16	17	18	19	20	21	22
23	24	25	26	27	28	29
30	31	1	2	3	4	5

August

30	31	1	2	3	4	5
6	7	8	9	10	11	12
13	14	15	16	17	18	19
20	21	22	23	24	25	26
27	28	29	30	31	1	2

September

27	28	29	30	31	1	2
3	4	5	6	7	8	9
10	11	12	13	14	15	16
17	18	19	20	21	22	23
24	25	26	27	28	29	30

October

1	2	3	4	5	6	7
8	9	10	11	12	13	14
15	16	17	18	19	20	21
22	23	24	25	26	27	28
29	30	31	1	2	3	4

Week 1

Our team was being recruited. Nothing happened this week!

Week 2

Our team was being recruited. Nothing happened this week!

Week 3

Our team was being recruited. Nothing happened this week!

Week 4

01-25: Kick Off Meeting

Gathering the team members and distribute the tasks... The adventure begins!

Week 5

01-30: 2th brainstorming: on the track of crocodile antimicrobial peptides...

We’ve took charge of differents accounts of the association (facebook, twitter, email, bank account, google drive…), and made a listing of 45 potential subjects for the competition.

Week 6

02-06: 3th brainstorming meeting

We’ve selected 17 subjects that we considered the most originals, the most feasible, and the most interesting. We’ve imagined to make a dressing against nosocomial infections containing bacteria that produces antimicrobial peptides from crocodile. We’ve read that crocodile peptides are not toxic for humans at microbicide quantity.

Week 7

02-16: 4th brainstorming

Only 7 subjects left! To make a safe device, we’ve thought of switch on the dressing by using spores that can be activated with the temperature.

02-13 to 02-17: Training course for INSA students at LISBP with our instructor Brice Enjalbert

Week 8

02-23: 5th brainstorming

We made a list of our public engagement events. For our subject, we’ve focused our research on the originality, and we’ve noted that a similar dressing has already been made by an iGEM team before. So we imagined the use of anticoagulant molecules in the dressing. We’ve thought of a bandage for pets.

Week 9

03-02: 6th brainstorming

We’ve analysed the 7 subjects, and we’ve noted some technical issues. For the “crocodile peptides project”, we’ve raised several questions : what organisme should we use to produce antimicrobial peptides? We need to find an organism which is not susceptible to their antimicrobial activity. We need to use a special membrane to contain GMOs inside the dressing and not directly in contact to the skin.

Week 10

03-05: Team building at the restaurant

Our team met for the first time outside the INSA buildings and shared a delicious korean meal after brainstorming the whole afternoon!

03-09: 7th brainstorming meeting

We’ve voted for our 4 favourite subjects : “crocodile antimicrobial peptides”, “bioluminescent living board”, “expiration pastille”, and “colourful cellular cycle”.

Week 11

03-12: Brainstorming in a café

03-14: 8th brainstorming

The problem of originality persists for the application: what about a suture thread? For the peptide-producer microorganism, a yeast could be a good solution because the antimicrobial activity of our peptide is bacterial-specific. For the activation of the peptide production, we’ve thought about lyophilisating the engineered yeast.

Week 12

03-21: 9th brainstorming

In view of what has already been done in iGEM for detection of quorum sensing molecules, it seems that we’ll need a bacteria to detect other quorum sensing molecules because it’s difficult to express a prokaryotic membrane-receptor into a yeast. So we’ve imagined a system with a prokaryote that detect a quorum sensing signal from a pathogenic bacteria, and that triggers peptide production by a yeast.

Week 13

03-26: Brainstorming in a café

03-30: 10th brainstorming

A second device is born: a shaker containing our too organisms (prokaryote-detector and yeast-peptide-producer) to detect and kill Vibrio cholerae in the water. We had a idea for our public engagement project: creating a pedagogic card game about microbiology. MicrobioWorld conception is in process...

Week 14

04-06: 11th brainstorming

Dropping of the first device because ALS detection of one single Staphylococcus aureus is going to be difficult. In contrary, Vibrio cholerae produces a high quantity of CAI-1, a specific quorum sensing molecule, at pathogenic quantity.

04-07: choice of the subject

We’ve finally choose to work on cholera water treatment using a prokaryotic-eukaryotic communication.

Week 15

04-13: 12th brainstorming

Actual solution to fight against cholera are not sufficient to prevent great epidemics, we need to contact NGOs to benefit from their experience of the field of a cholera epidemy. For the proof of concept, we need to find an effective cholera detector, and a good peptide producer. The detector bacteria could be E. coli, or maybe V. harveyi, that already possess the quorum sensing receptor of CAI-1, and all the proteins to do the phosphorylation cascade in response of CAI-1.

04-10 to 04-14 Training course for the UPS students at LBME with our instructors Anthony Henras and Yves Romeo

Week 16

04-19: Meeting with Marc Lemonnier, CEO of Antabio

We met Marc Lemonnier, founding CEO of ANTABIO: a start-up that develops novel therapies to treat drug-resistant infections by the most critical Gram-negative pathogens. This was our first meeting within the Entrepreneurship part of the project. The aim was to learn about the approach to follow to start a business and to have a first external opinion regarding the integration of our biological system into the market. You can read the result of his testimony here.

04-20: 13th brainstorming

We’ve found a company called “Sunwaterlife” that work on water treatment by filtrating water in Toulouse. We’ve planned to meet them to take advices from their experience, and ask if they could help us to build our device to contain GMMs into a membrane to avoid their dispersion into the environment. For the peptide-producer yeast, we’ve selected Pichia pastoris, famous for it’s capacity of fast production of proteins. To make a communication between our two organisms, we’ve thought of using diacetyl: our detector organism produces diacetyl in response of CAI-1 detection, that triggers AMP by P. pastoris.

Week 17

04-23: First drafts of Sobki, our mascot!

04-25:

Following our appointment with Marc Lemonnier, we began to think about a device so that our system could treat the water in a tank for villagers or the water of a flask for the tourists. The challenge is to confine the microorganisms so that they do not go into the water.

04-26: 14th brainstorming

For safety reasons we can’t use V. cholerae for our proof of concept, and we can’t use any gene from that bacteria. As V. harveyi is genetically close to V. cholerae but not pathogenic for human, we can use it’s quorum sensing molecule, the C8-CAI-1 that looks very much as the CAI-1, to mimic V. cholerae. Here, the idea is to engineer a bacteria to be a “cholerae-like bacteria” that produces a lot quorum sensing molecules to be detected by our detector V. harveyi.

04-27:

Téo and Margaux went to a conference about V. cholerae to learn more about genetics of that bacteria, and the main differences between V. cholerae and V. harveyi.

Week 18

05-02: 15th brainstorming

We need to evaluate the volume of water to treat. In the case of our device contain 1L water, it’s for only 1 person but we can imagine to treat a high volume for an entire village. We’ve prepared a list of questions to ask to NGOs to make a user friendly device.

05-07:

We’ve met 2 game designers and graphists students, Vincent and Julien, to help us in the conception of our card game about microbiology! They will make the graphism designs of our card game.

Week 19

05-11: 16th brainstorming

For the detection of diacetyl, we found the receptor Odr10, that activate the promoter pFUS for peptide production. For diacetyl production, the gene ALS is known to enhance diacetyl production, so we’ll need to express ALS gene in V. harveyi only when C8-CAI-1 is detected. This is complicated because the receptor of C8-CAI-1 induces a repression of the promoter pqrr4. We need to find a inhibitor regulation in our system.

05-13: Paul & Margaux officialy joined our team!

Week 20

05-17: 17th brainstorming

We planed to do NMR analysis to detect C8-CAI-1 and diacetyl for the proof of concept. We’ve asked to Bassler’s lab to give us 2 V. harveyi strains: a WT one, and the JMH626 strain deleted from the gene CqsA, which can’t produce C8-CAI-1 by itself. We’ll need that strain to detect C8-CAI-1 from our engineered “E. coli cholera-like”. Purified diacetyl has been commanded to do induction experiments on P. pastoris. We find the Tet on/ Tet off repression system for diacetyl production in response to C8-CAI-1.

05-18: iGEM distribution kit received

05-21 Sobki is born!

Week 21

We made a survey about cholera disease and about our project to know if people are aware of cholera epidemics in Africa and the existing solutions, and to know if people would be ready to use a device containing GMMs to treat the water they drink.

05-25: Parts design day at Brice's

Week 22

05-28: Official announcement of our subject

We officialy the subject of our project on social media!

05-30 and 31: Exposciences

We took part to “Exposciences” which is a scientific festival. During this event we made children extract banana DNA thanks to simple ingredients that they can find in their kitchen. We also discussed with them about microorganisms thanks to paper fortune teller and this support led them to be curious on the microscopic world around them.

06-01: Interventions in schools

The main goals of these interventions were the discovery of biology and research at school with two workshops: Microorganisms and their environment and growth of microorganisms on a Petri dish.

Experiments

Design and orders

Design of the gBlocks and launching of the synthesis order for IDT
Design of the PCR oligos

Design and orders

Design of the gBlocks and launching of the synthesis order for IDT
Design of the PCR oligos

Week 23

06-06: Meeting with the CEO of Sunwaterlife

We presented our project to Christophe Campéri-Ginestet who seems to be interested, especially by the detection function of V. cholerae. He would like to adapt it to the Sunwaterlife filters in order to have a direct colored response according to the water contamination. In addition, he would be willing to invest in our project in order to apply for JEI status (or “Young Innovative Enterprises” in english), a very interesting status for start-ups allowing them to have financial advantages. Besides, a collaboration is discussed so that Sunwaterlife provide us with their membranes, allowing us to confine our microorganisms within a device. He told us that he might be able to put us in touch with a UNICEF person in charge of cholera epidemics.

You can read the result of his testimony here.

06-08: 18th brainstorming

We’ve divided the subject in 3 modules. The “Quorum” module, with E. coli engineered to produce C8-CAI-1; the “Detection” module, with V. harveyi engineered to detect C8-CAI-1 and produce diacetyl in response; and the “Killing” module with P. pastoris engineered to detect diacetyl and produce crocodile AMP in response.

06-09: Intervention in school

The main goals of these interventions were the discovery of biology and research at school with two workshops: Microorganisms and their environment and growth of microorganisms on a Petri dish.

Experiments

Taking care of V. harveyi

Reception and storage of V. harveyi BB120 (WT) and V. harveyi JMH626 (ΔcqsA ΔluxQ ΔluxN) CmR at -80 °C.
Investigation of V. harveyi antibiotics resistances.

Week 24

06-13: Model

A first version of the SBGN (Systems Biology Graphical Notation) summarizing our synthetic system have been drawn.

06-14: Scope statement start

A scope statement was created for a device that treats the water of a tank and another for a device that treats the water in a flask.

06-15: 19th brainstorming

We’ve received our IDT parts! We finally choose the name of our project: Croc’n cholera.

06-16: Skype with Greece iGEM team

Experiments

Amplifying pSB1C3

Reception of IDT gBlocks and storage
Preparative work with the cloning vectors : Amplification and stock of pSB1C3

Taking charge of the lab and of V. harveyi

Growth kinetic assay in order to determine the adequate NaCl concentration for V. harveyi growth.
Reception of IDT gBlocks and storage
Transformation of DH5α competent cells with pYFP, pDsRed, pPIZα, pBR322 and amplification of the plasmids. The plasmids are double digested with the appropriate restriction enzymes.

Cloning

pPICzα, jmp62-DsRed (pDsRed) and jmp62-YFP (pYFP) were transformed into E.coli DHAα in order to amplify them.
Bacteria grown were then put into liquid culture to do miniprep.
Digestion BamHI/EcoRI to check pPICzα gel migration profile
Digestion XbaI/NheI to check jmp62-DsRed gel migration profile
Digestion BamHI/NheI to check jmp62-YFP gel migration profile

Week 25

06-19: Meeting with Doctors Without Borders, regional manager

She explained that the detection of cholera in the patient is simple because the symptoms are known. She told us that our system would be more useful treating water tanks in villages far from cholera camps rather than tourists. Indeed, governments often hide epidemics from tourists and tourist places take enough precautions to avoid tourists’ contamination.

You can read the result of her testimony here.

06-23: Beginning of the marketing approach

A marketing approach has been initiated to study the strengths and weaknesses of our two potential products and to determine the approach to sell them. Thanks to a SWOT analysis on both products, we realized that the product to treat water in a flask is not adapted to the market. This product is therefore abandoned and we focus on a product that processes a larger volume of water, suitable for villagers in the affected countries.

06-22 Skype with NUS iGEM team

06-23: 20th brainstorming

Meeting with all the team members and supervisors and final choice of the global device purpose: made for people living in remote areas in Africa, which are far from NGOs camps and more vulnerable to a cholera epidemy.

06-23: Model

Our mathematical model based on twelve ODEs was built!

06-23: First appearance of Sobki, our mascott!

Experiments

Plasmids preparation

Transformation of DH5α competent cells with pSB1C3 and amplification of the plasmids. The plasmids are double digested with the appropriate restriction enzymes.
Scale up of the preparative digestion, in order to have more DNA matrix.
Reception of the PCR primer.

Plasmids preparation

Transformation of DH5α competent cells with pGAP, pSB1C3 and amplification of the plasmids. The plasmids are double digested with the appropriate restriction enzymes.
Scale up of the preparative digestion, in order to have more DNA matrix.
Reception of the PCR primer.

Cloning

Preparative digestion of pPICZα with BamHI/BglII to clone our gene followed by a gel extraction to purify the open plasmid.
Glycerol stocks of E.coli DHAα containing pPICZα, pDSRed and pYFP were made after liquid culture.

Week 26

06-26: Skype with the iGEM team of Purdue

06-28: Meeting with the CEO of Sunwaterlife, building a collaboration

Really interested in the ability to detect V. cholerae in water, Christophe Campéri-Ginestet asked us to provide our bibliographic research and results at the end of the iGEM project. In addition, in exchange for our financial investment in our project, we have to fill out the JEI document which attests to our R&D activity for Sunwaterlife. He also put us in touch with Alama Keita, cholera leader of UNICEF in Niger. Finally, we talked to him about our product to contain our microorganisms. He helped us to design a cylindrical device whose sections would be these membranes to ensure GMMs containment.

You can read the result of his testimony here.

06-30: First steps of our ethical matrix

06-29: 21th brainstorming

We choose to made a Prezi for the presentation in Boston, the structure has been validated by instructors and students, and we made a first version of our poster for the meetup in Delft. Our wiki is online next day! Our survey has around 400 answers, so we can start analyses of results.

Experiments

Cloning VhCqsA

Amplification of the IDT gblocks and optimisation of the PCR cycle condition for VhCqsA part
Digestion of the PCR amplicons and ligation with the appropriate cloning vector.

Assembly of V. harveyi gene circuit : starting the sub-cloning work

Amplification of the IDT gblocks and optimisation of the PCR cycle condition for VhCqsA part
Digestion of the PCR amplicons and ligation with the appropriate cloning vector.
Cloning attempt #1 and #2 to sub-clone Vh1, Vh2 and Vh3 in their respective vector.

Cloning

PCR of DsRed and YFP with primers containing restriction sites. PCR products for these genes have right size. PCR products are purified.
PCR of our parts (cOT2 ; leucrocine ; DNY15; Odr10) from IDT GBlocks. All PCRs succeeded.
cOT2 ; leucrocine ; DNY15 ; Odr10 were digested BamHI/BglII to be inserted in pPICZα.
Transformation of pPICZα-cOT2 ; pPICZα-leucrocine ; pPICZα-Odr10 and pPICZα-DNY15 in E. coli DH5α
Miniprep of liquid culture transformants
Digestion BmaHI/BglII to check the gel migration profile.
→ It appears that the plasmid was well digested but the insert does not appear. There is an intense band around 1300bp that might correspond to supercoiled plasmid. To conclude it seems like pPICZα closed itself without the insert: BamHI and BglII are compatibles.
New primers were ordered replace by PCR the BglII site by KpnI site to remove the possibility for the plasmid to close itself.

Week 27

07-06: 22th brainstorming

We’ve prepared some public engagement events for september : an exhibition at the campus science in Toulouse to showcase ancient iGEM Toulouse projects, and synthetic biology, a practical work in an high school, and we’ve planned to participate to the “European Researcher’s Night” with the LISBP by creating a game called “Possible or Impossible”, to open a dialogue about GMO legislation with people.

07-07: Delft European iGEM Meetup

Experiments

Cloning VhCqsA

Optimization of PCR cycle
Cloning of VhCqsA into pSB1C3 : positives clones grew

Assembly of V. harveyi gene circuit : continuing the sub-cloning work

Assembly of V.harveyi gene circuit: Analysis of the transformants of V.harveyi module (attempt #1 and #2) by checking of the restriction map.
New attempt (attempt #3) to sub-clone Vh1, Vh2 and Vh3 in their respective vector.

Cloning

While waiting for the new primers, another try was made using for the ligation a 1:10 ratio (pPICZα:insert).
Transformants appeared in all plates except for Odr10.
Miniprep and digestions of transformants to check the gel migration profile.
We obtained 2 clones pPICZα-DNY15. The gel confirmed that D1 and D2 are clones with the entire plasmid pPICZa A open only in BamHI site with one insert in this site. D1 has the insert in the good direction and D2 has the insert in the opposite direction.
Clones D1 and D2 were stored in glycerol.
At the end of the week, PCR with new primers containing KpnI instead of BglII were made for all the parts (cOT2 ; leucrocine ; DNY15 ; Odr10). All the PCR worked perfectly, PCR tubes were pooled. Odr10 has secondary products which need gel extraction.
Digestion of plasmid and parts with KpnI and BamHI

Week 28

07-13: 23th brainstorming

Citation from Cédric : “we must be humble before molecular biology”. Our card game conception progressing well! We find a name : MicrobioWorld, and the main rules has been determined after several crazy gameplay!

07-14: Meeting with Doctors Without Borders, cholera specialist

Fransisco Luquero assured us that the current solutions (bleach, boil water) were not the most suitable to treat water from cholera. Our device could be interesting if it is suitable to treat several liters of water and even if it contains GMM, it could be easily accepted by the population as long as it does not change the color and taste of water.

Experiments

Assembly of V. harveyi gene circuit : continuing the sub-cloning work

Assembly of V.harveyi gene circuit: Analysis of the transformants of V.harveyi module (attempt #1 and #2) by checking of the restriction map.
New attempt (attempt #3) to sub-clone Vh1, Vh2 and Vh3 in their respective vector.

Cloning

Ligation and transformation of pPICZα-cOT2 ; pPICZα-leucrocine ; pPICZα-Odr10 and pPICZα-DNY15.
Miniprep of transformants and gel migration were made. The ladder was clear but there was no DNA on tranformants colums.
Nanodrop analysis showed that there was not enough DNA.
→ The hypothesis is that the liquid culture was too long so E. coli lost the plasmid.

Experiments with P. pastoris

We did plates of YPD with increasing concentration of zeocin to select P. pastoris clones which integrated the most copies of the construction.
Preparation of competent yeast (Pichia pastoris)
Digestion of D1 clone (pPICZα-DNY15) with HindIII to integrate the plasmid construction at the AOX1 promotor in P. pastoris genome.
Electroporation of competent yeast with the digested DNA.

Week 29

07-17: Model

Our system of ODEs script and function have been developed in Matlab.

07-20: 24th brainstorming

We’ve finished a first version of our presentation, which will be presented during the Parisian Meetup. The Matlab programming is done, the next step is to add values from bibliography.

07-20: First meeting with Pierre-Alain Hoffmann

We met Pierre-Alain Hoffmann, Deputy Director of CRITT Bio-Industries for the business plan elaboration. The knowledge in creation and development of companies of Pierre-Alain Hoffmann allowed us to understand the goal of a business plan. He accompanied us in this approach, explaining the points to be detailed in order to better integrate our system in the market.

07-21: Parisian meetup

Experiments

Expressing VhCqsA (2nd)

Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR (fail)
SDS page fo the protein overexpression of our clones (fail)

Assembly of V. harveyi gene circuit : A new strategy

Amplification of the IDT gblocks, digestion of the PCR amplicons.
Amplification and digestion of the sub-cloning vectors.
Ligation and transformation in E. coli STELLAR competent cells (attempt #8).

Cloning

This time transformation were made with E. coli XL1 Blue which are supposed to be more efficient.
No DNA after gel migration like the previous week.

Experiments with P. pastoris

Colony PCR of P. pastoris clone which grew on YPD zeocin. → All clones have the gene construction in their genome!
The 11 clones of Pichia were grown on plates with 250 µg/mL, 500 µg/mL and 1000 µg/mL to see which one has the best integration of the construction. All clones grew onto 250, 500 and 1000 µg/mL zeocin.

Week 30

07-23: Skype with the iGEM team of Greece

07-27: Meeting with Pierre Monsan at TWB

As a preindustrial demonstrator recognized in France, TWB helps many projects to become companies. We presented them our project and had a positive feedback. It is even one of our biggest sponsors.

You can read the result of their testimony here.

07-29: Team building in Pyrenees

Experiments

Expressing VhCqsA (3rd)

Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR (fail)

Assembly of V. harveyi gene circuit : The first transformant

TA cloning (attempt #9): amplification of the IDT gblocks with KAPA2G polymerase , digestion of the PCR amplicons. Ligation with pGEM vector TA cloning vector.
Analysis of the transformants (attempt #8 and #9) by checking the restriction map.
The part 1 and 2 were successfully cloned in pBR322 and pGEM.

Cloning

PCR were made again of all parts in order to have enough materials to do ligation.
This time lot of DNA was digested.
Several strategies were tried to obtain pPICZα-cOT2 ; pPICZα-leucrocine and pPICZα-Odr10 with DHAα and XL1 Blue strains.
At the end of the week we got 2 clones of pPICZα-Odr10 in E. coli DHAα

Experiments with P. pastoris

The supernatant of P. pastoris containing the DNY15 gene was extracted and then concentrated via rotavapor and freeze-drying.
In a 24 wells plate, kinetic growths of V. harveyi were followed with or without P. pastoris supernatant to check the bactericide effect of DNY15. → this first try was not perfectly made
Inhibition test with the same supernatant was made on petri dish. → this first try was not perfectly made

Week 31

08-01: 25th brainstorming

Our collaborations with other iGEM teams are in process! We’ve started an experiment for iGEM Purdue.

Experiments

Expressing VhCqsA (aborted) + Cloning of VcCqsA

Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR aborted : no growth of bacteria

Step 2 : reconstruction of CqsS Rc by ligating Vh2 to Vh1 in pBR322.

Storage of the transformants with Vh1 and Vh2 in glycerol at -80°C.
Amplification, digestion of pBR322-Vh1, pGEM-Vh2 and ligation in E. coli TOP10 competent cells (attempt A)
Transformation of Vh3 (attempt #10) in E. coli DH5α competent cells
Analysis of the transformants (attempt #A and #10) by checking the restriction map.

Cloning

Different strategies were made again to obtain pPICZα-cOT2 and pPICZα-leucrocine in E.coli. → Several clones of pPICZα-cOT2 and pPICZα-leucrocine were obtained!!!
Liquid culture of DHAα containing pSB1C3 was made to get enough material to do cloning of our parts in it.
Ligation of YFP in pPICZα-Odr10 and in pPICZα-DNY15 instead of the AMP gene.
Integration of AMP genes in pSBIC3 (=iGEM parts).

Experiments with P. pastoris

Cytotoxicity test of DNY15 was tried again with kinetic growths of V. harveyi.
Cytotoxicity test of DNY15 was tried again on petri dish: No effect of DNY15 can be seen.
Genomic integration of pPICZα-cOT2 , pPICZα-leucrocine and pPICZα-Odr10.

Week 32

08-07: Skype with the iGEM team of Boston

08-07: Model

All the experimental and literature values have been implemented in our Matlab script, which provides promising results. These first results will be used for preliminary work in design and entrepreneurship.

Experiments

Expressing VhCqsA (4th time) + VcCqsA cloning

Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR (fail)
PCR on VcCqsA

Step 2 CqsS Rc assembly in progress…still no success for Vh3

Amplification, digestion of pBR322-Vh1 pGEM-Vh2 and ligation in E. coli TOP10 competent cells (attempt B and C)
Transformation of Vh3 (attempt #11 to #13) in E. coli TOP10 competent cells using an alternative restriction enzyme : PstI-HF restriction enzyme is remplaced by SpeI.

Cloning

We obtained the YFP gene after the GAP promotor and the FUS1 promotor in E. coli.

Experiments with P. pastoris

pGAP-YFP and pFUS-YFP were transformed in P. pastoris.
P. pastoris clones were obtained with pPICZα-Odr10 ; pPICZα-leucrocine and pPICZα-cOT2 but the PCR colony didn’t work. After several trials and controls we concluded that the DNA extraction the way we did it was not enough to do a PCR of the GAP promotor in the pichia genome.
A kinetic study of V. harveyi growth was made using pichia supernatant. → A difference was observed but the experiment needs to be done again.

Week 33

08-16: Skype with the iGEM team of Groningen

08-17: “iGEM on ice”

Experiments

Cloning of VcCqsA

From ligation to transformation : transformants grew.

Step 2 CqsS Rc assembly in progress, preparation of the conjugation

Analysis of the transformants (attempt #13) by checking the restriction map : the part 3 is successfully cloned in pSB1C3. The transformant is stored in glycerol at -80°C.
Amplification of pBBR1MCS-4 and pBBR1MCS-5 for cloning of Vh3 and for a conjugation assay.
Ligation of Vh3 in pBBR1MCS-4 and transformation in E. coli TOP10 (attempt α).
Amplification, digestion of pBR322-Vh1 pGEM-Vh2 and ligation in E. coli TOP10 competent cells (attempt D to F)

P. pastoris clones were obtained with the YFP reporter behind both promotor: GAP and FUS1.
Fluorescence test was made but it didn’t work.
A cytotoxicity test was made on V.harveyi with pichia-AMP supernantant but we had trouble because of volumes used.
We tried a new DNA extraction protocol to do the PCR colony on P. pastoris and it did work.

Week 34

08-24: 26th brainstorming

We finally choose our track! We will be candidate in “information processing”, to highlight the prokaryote-eukaryote communications in our multimicrobial system!

Experiments

Confirming VcCqsa clone + Expressing VhCqsA (5th time)

Transformant were good
Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR (fail)

Analysis of the transformants (attempt #F) by checking the restriction map : the part Vh1+Vh2 is successfully cloned in pBR322. The transformant is stored in glycerol at -80°C.
Amplification, digestion of pBR322-Vh1+Vh2, pSB1C3-Vh3. Ligation and transformation (attempt #1 and #2) in E. coli TOP10 competent cells.
Ligation of Vh3 in pBBR1MCS-4 and transformation in E. coli TOP10 (attempt β and γ)
Vh3 part validation : diacetyl production assay by NMR detection with E. coli BL21.

Cytotoxicity test was made again but this time on a petri dish. We had a halo for the antibiotic but P. pastoris’ supernatant didn’t stop V. harveyi’s growth.
We’ve tried to characterize pFUS1 promoter in different media but we got no positive result.

Week 35

08-28: First try for the device modeling

A 3D modeling of the device was initiated on the Solidworks software with the help of a professional in the design of industrial parts. The objective is to model the device in 3D and then to print the prototype.

08-31: 27th brainstorming

After much thinking, we are agreed that we want Margaux, Maxant and Margaux to be our speakers during the competition in Boston!

08-31: Model

First MCA results! The control exerted by each parameter on the response time can now be analysed.

Experiments

Expressing VhCqsA (6th time) + Bioluminescence

Production of C8-CAI-1 in minimal media, extration on LLE dichloromethane and analysis on NMR (fail)
Optimisation of Vibrio harveyi JMH626 bioluminescent assay

2nd diacetyl production assay by NMR detection with E. coli BL21.
Success of the conjugation assay of pBBR1MCS-4.

This time, we tested the production of DNY15 in YPD with 40g/L of glucose instead of 30g/L because publications which use pGAP as a promotor to produce AMP sometimes have a higher production with more glucose.
Cytotoxicity tests using the supernatant from the 40g/L glucose culture media was made. Supernatants were filtered beforehand and half was freeze-dried. We got a weak halo for the DNY15 production in glucose 40 and supernatant freeze-dried!

Week 36

09-04: Start of our Ulule campaign

09-07: 28th brainstorming

09-08: Farewell party

09-09: Meeting with Marie-Pierre Escudié

Experiments

Expressing VhCqsA (7th time)

Production of C8-CAI-1 in minimal media, and analysis on MS (fail) extration on LLE dichloromethane and analysis on NMR (fail)

3rd Diacetyl production assay by NMR detection with E. coli MG1655.
Amplification of pBBR1MCS-4 and pBBR1MCS-5.

Because we still had trouble integrating some parts in P. pastoris’ genome we tried the integration via electroporation and via chemical way at the same time.
We launched one again P. pastoris culture in YPD 40g/L and 50g/L glucose to try producing more DNY15.

Week 37

09-14: 29th brainstorming

We’ve prepared the abstract, and choose a subtitle : “a synthetic microbial consortium”

Experiments

Bioluminescence assay

Trial on the expression of C8-CAI-1 with induction on bioluminescence on liquid media : fail

4th diacetyl production assay by NMR detection with E. coli MG1655 in M9 media supplemented with xylose and pyruvate.
Transformation of E. coli TOP10 competent cells with GFP from iGEM kit
Ligation and transformation of pBBR1MCS-4 to RFP from iGEM kit in E. coli TOP10 competent cells for conjugation to V. harveyi.
Preparation of the clones for sequencing.

We prepared sample to sequence the AMP genes we wanted to give to the iGEM registry.
Culture media from P. pastoris culture of last week were freeze-dried. And the cytotoxicity test on petri dish was made again but this time, even the positive control with antibiotic didn’t work.
P. pastoris clones were obtained with pAOX1-leucrocine, pAOX1-cOT2, Odr10-pFUS-RFP.

Week 38

09-21: 30th brainstorming

We’ve finished the experiment time, it’s time to write results for the wiki!

09-22: Meeting with UNICEF

He warned us that NGOs would agree to use a GMM system only if efficacy is proven and there is no danger of GMM proliferation. He also gave us an idea of the volume of water a village needs. Moreover, it is also important to make a system with as little waste as possible.

You can read the result of Alama Keita's testimony here.

Experiments

Bioluminescence assay

Trial on the expression of C8-CAI-1 with induction on bioluminescence on plate : success

Results of the 4th Diacetyl production assay by NMR detection: a small peak of diacetyl is visible.
5th diacetyl production assay by NMR detection with E. coli MG1655 in M9 media supplemented with xylose and pyruvate.
Results of ligation of RFP in conjugative plasmid: all transformants are red. Two transformants are stored at -80°C in order to be used for conjugation assay.
Preparative work for the ligation of Vh3 in pBBR1MCS-4 with fresh material

AMP genes were sent to be sequenced.
P. pastoris producing DNY15 was cultured to do a RT-qPCR experiment to compare it to the WT P. pastoris.
New cytotoxicity tests on petri dish were made but this time it didn’t work because of the paper disk we used. Once again the positive control didn’t work.
Cultures in BMMY adding methanol every 24h for 120h were made for both pAOX1-leucrocine and pAOX1-cOT2 P.pastoris clones. Half of each supernatant was stored at 4°C and the other half was evaporated to concentrate 10 times.

Week 39

09-27: 31th brainstorming

We’ve started to make rehearsals for the presentation in Boston.

09-29: European Researcher's Night

We’ve been to the European Researcher’s Night, a major scientific event that gather researchers and general public in a convivial atmosphere. In 2017, the topic of the event was “Impossible”. In order to follow this thematic, we chose to focus our workshop on the incredible features of biodiversity, how to use it in synthetic biology, and what are legal limits on GMO use in France.

Experiments

Observation by microscopy of V. harveyi (rfp) transformant
Ligation of pBBR1MCS-4 and Vh3 at 16°C overnight, and transformation in competent E. coli (Stellars)

New cytotoxicity tests on petri dishes were made with the leucrocine and cOT2 supernatants. This time with appropriate discs. Positive control did not work again.
New cytotoxicity tests on petri dishes were made but using pure chloramphenicol. A weak halo was seen. We found out that the V. harveyi strain we used to do cytotoxicity test was maybe! the JMH626 one which was chloramphenicol-resistant.
RT-PCR experiment was made.
To test the Odr10-RFP system and activate pFUS, nitrogen depletion cultures were done and two sources of nitrogen were tested: sulfate ammonium and glutamine. The fluorescence following was made in a plate reader.
New cytotoxicity tests on petri dishes were made starting from a fresh V. harveyi cuture. Leucrocine, cOT2 and D-NY15 (40g/L and 50g/L glucose) productions were tested. Halos are clearly visible for D-NY15 40g / L not concentrated and concentrated 10 times.
A 24 well plate was launched by adding leucrocine, cOT2 and D-NY15 supernatant to V. harveyi cultures in exponential phase.

Week 40

10-02: Meeting with Pierre-Alain Hoffmann, director of CRITT Bio-industries.

Between these two appointments, we have elaborated the business plan ourselves. Pierre-Alain Hoffmann read it and made a feed-back. He was pleased with our work, ensuring that some start-ups do not start their business with such a business plan.

10-05: 32th brainstorming

Rehearsals, and writing of different pages of the wiki.

10-04: High school practical work

We prepared an intervention for High School senior students in scientific classes. Our involvement was focused on 3 main goals: implement a learner-centred pedagogical approach in a high school class, make discover biotechnologies through a practical approach, and discuss on “how to be part of science, and which paths to choose after high school?”.

Week 41

33th brainstorming

Rehearsals, and writing of different pages of the wiki.

All week: exhibition at Bib'INSA

In order to make former iGEM Toulouse projects understandable for all scientist student (and not only for biologists), we redesigned posters of previous project E. calculus, SubtiTree, ApiColi, Paleotilis, and of our project Croc’n Cholera.

Week 42

10-10: End of the 3D modeling of the device and 3D printing

The device was printed in 3D by the High school Georges Cabanis at Brive-La-Gaillarde.

10-11: Model

Some inaccuracies in our mathematical model have been corrected, we have now the final version of all our Matlab files! Results will be used for our entrepreneurship and design work.

10-13

We have now our final MCA, and performed a global analysis of our system. We can now definitely conclude about sensitivity and robustness of our model.

13-10: Seminar at the campus science Toulouse

After working during several months on synthetic biology for our iGEM project, we needed to organize an event to present our work and the one achieved by the previous iGEM teams. We also wanted to have an exchange of opinions with people who possibly have different values and considerations than us. The intended public was mainly composed of the Paul Sabatier university and INSA Toulouse students, two of the schools we are intending.

Week 43

10-21: Model

An intuitive modeling interface have been built for displaying our model results to non-specialists.

Week 44

Realisations pages
Overview	Notebook	Clonings	Results	Contribution	Protocols	Safety

@@ Line 4: / Line 4: @@
 <html>
-<style>
-main{
-  position:relative;
-  overflow: auto;
-  height:100%;
-}
-.main_content{
-  position:fixed;
-  top:90px;
-  right:0px;
-  left:0px;
-  bottom:0px;
-  overflow:auto;
-  background-image: linear-gradient(45deg, #4296c1 0%, #e4efe9 100%);
-}
-.middle_container{
-  padding-bottom: 80px;
-}
-.section_container{
-  width:90%;
-  min-height:100%;
-  margin:0px auto;
-  position:relative;
-}
-@media screen and (max-width: 900px){
-    .section_container{
-      width:100%;
-    }
-  }
-section{
-  background-color: rgba(255,255,255,0.2);
-  padding:3% 6%;
-  text-align: justify;
-  border-radius:20px;
-  position:relative;
-  margin-top:50px;
-}
-.main_title{
-  height:300px;
-  font: 700 4em/1.5 'Quicksand', sans-serif;
-  position:relative;
-  letter-spacing: 0.1em;
-  z-index:10;
-  margin-bottom:50px;
-  width:100%;
-}
-.main_title > div{
-  width:100%;
-  position:absolute;
-  bottom:-10px;
-  background:rgba(255,255,255,0.2);
-  border-radius: 20px;
-}
-.main_title p{
-  padding:30px;
-}
-.main_title img{
-  position:absolute;
-  right:0;
-  width:400px;
-  bottom:-25px;
-}
-section h1{
-      font-family: 'Quicksand', sans-serif;
-      font-size:34pt;
-      margin-top:-75px;
-      text-align: right;
-     }
-</style>
@@ Line 91: / Line 12: @@
    <div class="section_container">
-     <section style="margin-top:200px;">
+     <section style="display:table;background:none;padding:0px !important;z-index:100; ">
-      <h1 style="text-align: left; font-size:40pt;">Notebook</h1>
+       <h1 style="vertical-align:bottom;display:table-cell; width:50%;font-size:60pt;letter-spacing: 0.2em;z-index:120;text-align: center;">Notebook</h1>
+       <img style="vertical-align:bottom;display:table-cell; width:100%;" src="https://static.igem.org/mediawiki/2017/5/53/T--INSA-UPS_France--Labbook.png" alt="">
+    </section>
+    <section style="margin-top:50px;">
        <p>
          Here is a summary of what we did for our project, week by week.
          You can go on our <a href="https://2017.igem.org/Team:INSA-UPS_France/HP/Silver">Human Practices labbook</a> page to know more about how each event affected us to take a decision about the future of our project.
        </p>
+    </section>
+    <section style="background:none;margin:0;">
+      <div>
+        <div style="display:inline-block;background:#ffd966; width:20px; height:20px"></div>
+        <div style="display:inline-block;">Brainstorming meeting</div>
+      </div>
+      <div>
+        <div style="display:inline-block;background:#8e7cc3; width:20px; height:20px"></div>
+        <div style="display:inline-block;">Team day</div>
+      </div>
+      <div>
+        <div style="display:inline-block;background: #d5a6bd; width:20px; height:20px"></div>
+        <div style="display:inline-block;">Meetup with other iGEM teams</div>
+      </div>
+      <div>
+        <div style="display:inline-block;background:#f6b26b; width:20px; height:20px"></div>
+        <div style="display:inline-block;">Public Engagement event</div>
+      </div>
+      <div>
+        <div style="display:inline-block;background:#e06666; width:20px; height:20px"></div>
+        <div style="display:inline-block;">Integrated Human Practices / Entrepreneurship major step</div>
+      </div>
+      <div>
+        <div style="display:inline-block;border-left:solid 5px #0097a7; background:#eee; width:15px; height:20px"></div>
+        <div style="display:inline-block;">Experiments</div>
+      </div>
      </section>
@@ Line 271: / Line 226: @@
          </tr>
          <tr data-target="7">
-           <td>12</td>
+           <td class="experiment_day">12</td>
-           <td>13</td>
+           <td class="experiment_day">13</td>
-           <td>14</td>
+           <td class="experiment_day">14</td>
-           <td>15</td>
+           <td class="experiment_day">15</td>
-           <td class="meeting_day">16</td>
+           <td class="meeting_day experiment_day">16</td>
-           <td>17</td>
+           <td class="experiment_day">17</td>
-           <td>18</td>
+           <td class="experiment_day">18</td>
          </tr>
          <tr data-target="8">
@@ Line 372: / Line 327: @@
        </tr>
        <tr data-target="15">
-         <td>9</td>
+         <td  class="experiment_day">9</td>
-         <td>10</td>
+         <td class="experiment_day">10</td>
-         <td>11</td>
+         <td class="experiment_day">11</td>
-         <td>12</td>
+         <td class="experiment_day">12</td>
-         <td class="meeting_day">13</td>
+         <td class="meeting_day experiment_day">13</td>
-         <td>14</td>
+         <td class="experiment_day">14</td>
-         <td>15</td>
+         <td class="experiment_day">15</td>
        </tr>
        <tr data-target="16">
@@ Line 392: / Line 347: @@
          <td class="team_day"s>23</td>
          <td>24</td>
-         <td>25</td>
+         <td class="ihp_day">25</td>
          <td class="meeting_day">26</td>
-         <td>27</td>
+         <td class="team_day">27</td>
          <td>28</td>
          <td>29</td>
@@ Line 419: / Line 374: @@
            <td>3</td>
            <td>4</td>
-           <td>5</td>
+           <td  class="pe_day">5</td>
            <td>6</td>
          </tr>
@@ Line 443: / Line 398: @@
          <tr data-target="21">
-           <td>21</td>
+           <td  class="team_day">21</td>
            <td>22</td>
            <td>23</td>
@@ Line 581: / Line 536: @@
            <td class="lastmonth">30</td>
            <td class="lastmonth">31</td>
-           <td class="meeting_day">1</td>
+           <td class="meeting_day experiment_day">1</td>
-           <td>2</td>
+           <td class="experiment_day">2</td>
-           <td>3</td>
+           <td class="experiment_day">3</td>
-           <td>4</td>
+           <td class="experiment_day">4</td>
-           <td>5</td>
+           <td class="experiment_day">5</td>
          </tr>
          <tr data-target="32">
-           <td>6</td>
+           <td class="experiment_day">6</td>
-           <td class="igemers_day">7</td>
+           <td class="igemers_day experiment_day">7</td>
-           <td>8</td>
+           <td class="experiment_day">8</td>
-           <td>9</td>
+           <td class="experiment_day">9</td>
-           <td>10</td>
+           <td class="experiment_day">10</td>
-           <td>11</td>
+           <td class="experiment_day">11</td>
-           <td>12</td>
+           <td class="experiment_day">12</td>
          </tr>
          <tr data-target="33">
-           <td>13</td>
+           <td class="experiment_day">13</td>
-           <td>14</td>
+           <td class="experiment_day">14</td>
-           <td>15</td>
+           <td class="experiment_day">15</td>
-           <td class="igemers_day">16</td>
+           <td class="igemers_day experiment_day">16</td>
-           <td class="team_day">17</td>
+           <td class="team_day experiment_day">17</td>
-           <td>18</td>
+           <td class="experiment_day">18</td>
-           <td>19</td>
+           <td class="experiment_day">19</td>
          </tr>
          <tr data-target="34">
-           <td>20</td>
+           <td class="experiment_day">20</td>
-           <td>21</td>
+           <td class="experiment_day">21</td>
-           <td>22</td>
+           <td class="experiment_day">22</td>
-           <td>23</td>
+           <td class="experiment_day">23</td>
-           <td class="meeting_day">24</td>
+           <td class="meeting_day experiment_day">24</td>
-           <td>25</td>
+           <td class="experiment_day">25</td>
-           <td>26</td>
+           <td class="experiment_day">26</td>
          </tr>
          <tr data-target="35">
-           <td>27</td>
+           <td class="experiment_day">27</td>
-           <td>28</td>
+           <td class="experiment_day">28</td>
-           <td>29</td>
+           <td class="experiment_day">29</td>
-           <td>30</td>
+           <td class="experiment_day">30</td>
            <td class="meeting_day">31</td>
            <td class="lastmonth">1</td>
@@ Line 635: / Line 590: @@
            <td class="lastmonth">30</td>
            <td class="lastmonth">31</td>
-           <td>1</td>
+           <td class="experiment_day">1</td>
-           <td>2</td>
+           <td class="experiment_day">2</td>
          </tr>
          <tr data-target="36">
-           <td>3</td>
+           <td class="experiment_day">3</td>
            <td class="pe-event_day">4</td>
-           <td>5</td>
+           <td class="experiment_day">5</td>
-           <td>6</td>
+           <td class="experiment_day">6</td>
-           <td class="meeting_day">7</td>
+           <td class="meeting_day experiment_day">7</td>
-           <td class="team_day">8</td>
+           <td class="team_day experiment_day">8</td>
-           <td class="ihp_day">9</td>
+           <td class="ihp_day experiment_day">9</td>
          </tr>
          <tr data-target="37">
-           <td>10</td>
+           <td class="experiment_day">10</td>
-           <td>11</td>
+           <td class="experiment_day">11</td>
-           <td>12</td>
+           <td class="experiment_day">12</td>
-           <td>13</td>
+           <td class="experiment_day">13</td>
-           <td class="meeting_day">14</td>
+           <td class="meeting_day experiment_day">14</td>
-           <td>15</td>
+           <td class="experiment_day">15</td>
-           <td>16</td>
+           <td class="experiment_day">16</td>
          </tr>
          <tr data-target="38">
-           <td>17</td>
+           <td class="experiment_day">17</td>
-           <td>18</td>
+           <td class="experiment_day">18</td>
-           <td>19</td>
+           <td class="experiment_day">19</td>
-           <td>20</td>
+           <td class="experiment_day">20</td>
-           <td class="meeting_day">21</td>
+           <td class="meeting_day experiment_day">21</td>
-           <td class="ihp_day">22</td>
+           <td class="ihp_day experiment_day">22</td>
-           <td>23</td>
+           <td class="experiment_day">23</td>
          </tr>
          <tr data-target="39">
-           <td>24</td>
+           <td class="experiment_day">24</td>
-           <td>25</td>
+           <td class="experiment_day">25</td>
-           <td>26</td>
+           <td class="experiment_day">26</td>
-           <td class="meeting_day">27</td>
+           <td class="meeting_day experiment_day">27</td>
-           <td>28</td>
+           <td class="experiment_day">28</td>
-           <td class="pe-event_day">29</td>
+           <td class="pe-event_day experiment_day">29</td>
-           <td>30</td>
+           <td class="experiment_day">30</td>
          </tr>
        </table>
@@ Line 915: / Line 870: @@
            Only 7 subjects left! To make a safe device, we&rsquo;ve thought of switch on the dressing by using spores that can be activated with the temperature.
          </p>
+      </section>
+      <section class="week-experiment">
+        <h2>02-13 to 02-17: Training course for INSA students at LISBP with our instructor Brice Enjalbert</h2>
        </section>
      </div>
@@ Line 958: / Line 916: @@
        <section class="week-meeting">
          <h2>03-09: 7th brainstorming meeting</h2>
-         <p><i>
+         <p>
-          Dropping of first device
+           We&rsquo;ve voted for our 4 favourite subjects : &ldquo;crocodile antimicrobial peptides&rdquo;, &ldquo;bioluminescent living board&rdquo;, &ldquo;expiration pastille&rdquo;, and &ldquo;colourful cellular cycle&rdquo;.
-         </i></p>
+         </p>
        </section>
      </div>
@@ Line 975: / Line 933: @@
        <section class="week-meeting">
          <h2>03-14: 8th brainstorming</h2>
+        <p>
+          The problem of originality persists for the application: what about a suture thread? For the peptide-producer microorganism, a yeast could be a good solution because the antimicrobial activity of our peptide is bacterial-specific. For the activation of the peptide production, we&rsquo;ve thought about lyophilisating the engineered yeast.
+        </p>
        </section>
      </div>
@@ Line 986: / Line 947: @@
        <section class="week-meeting">
          <h2>03-21: 9th brainstorming</h2>
+        <p>
+          In view of what has already been done in iGEM for &nbsp;detection of quorum sensing molecules, it seems that we&rsquo;ll need a bacteria to detect other quorum sensing molecules because it&rsquo;s difficult to express a prokaryotic membrane-receptor into a yeast. So we&rsquo;ve imagined a system with a prokaryote that detect a quorum sensing signal from a pathogenic bacteria, and that triggers peptide production by a yeast.
+        </p>
        </section>
      </div>
@@ Line 1,000: / Line 964: @@
        <section class="week-meeting">
          <h2>03-30: 10th brainstorming</h2>
-         <p><i>
+         <p>
-          Technical solutions
+          A second device is born: a shaker containing our too organisms (prokaryote-detector and yeast-peptide-producer) to detect and kill <i>Vibrio cholerae</i> in the water. We had a idea for our public engagement project: creating a pedagogic card game about microbiology. <i>MicrobioWorld</i> conception is in process...
-        </i></p>
+        </p>
        </section>
      </div>
@@ Line 1,014: / Line 978: @@
        <section class="week-meeting">
          <h2>04-06: 11th brainstorming</h2>
-         <p><i>
+         <p>
-           Second device proposition
+           Dropping of the first device because ALS detection of one single Staphylococcus aureus is going to be difficult. In contrary, <i>Vibrio cholerae</i> produces a high quantity of CAI-1, a specific quorum sensing molecule, at pathogenic quantity.
-        </i></p>
+        </p>
        </section>
        <section class="week-team">
          <h2>04-07: choice of the subject</h2>
+        <p>
+          We&rsquo;ve finally choose to work on cholera water treatment using a prokaryotic-eukaryotic communication.
+        </p>
        </section>
      </div>
@@ Line 1,031: / Line 998: @@
        <section class="week-meeting">
          <h2>04-13: 12th brainstorming</h2>
+        <p>
+          Actual solution to fight against cholera are not sufficient to prevent great epidemics, we need to contact NGOs to benefit from their experience of the field of a cholera epidemy. For the proof of concept, we need to find an effective cholera detector, and a good peptide producer. The detector bacteria could be <i>E. coli</i>, or maybe <i>V. harveyi</i>, that already possess the quorum sensing receptor of CAI-1, and all the proteins to do the phosphorylation cascade in response of CAI-1.
+        </p>
+      </section>
+      <section class="experiment-meeting">
+        <h2>04-10 to 04-14 Training course for the UPS students at LBME with our instructors Anthony Henras and Yves Romeo</h2>
        </section>
      </div>
@@ Line 1,043: / Line 1,016: @@
          <h2>04-19: Meeting with Marc Lemonnier, CEO of Antabio</h2>
          <p>
-           You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a1">his testimony here.</a>
+           We met Marc Lemonnier, founding CEO of ANTABIO: a start-up that develops novel therapies to treat drug-resistant infections by the most critical Gram-negative pathogens. This was our first meeting within the Entrepreneurship part of the project. The aim was to learn about the approach to follow to start a business and to have a first external opinion regarding the integration of our biological system into the market. You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a1">his testimony here.</a>
          </p>
        </section>
        <section class="week-meeting">
          <h2>04-20: 13th brainstorming</h2>
+        <p>
+          We&rsquo;ve found a company called &ldquo;Sunwaterlife&rdquo; that work on water treatment by filtrating water in Toulouse. We&rsquo;ve planned to meet them to take advices from their experience, and ask if they could help us to build our device to contain GMMs into a membrane to avoid their dispersion into the environment. For the peptide-producer yeast, we&rsquo;ve selected <i>Pichia pastoris</i>, famous for it&rsquo;s capacity of fast production of proteins. To make a communication between our two organisms, we&rsquo;ve thought of using diacetyl: our detector organism produces diacetyl in response of CAI-1 detection, that triggers AMP by <i>P. pastoris</i>.
+        </p>
        </section>
      </div>
@@ Line 1,057: / Line 1,033: @@
        </section>
        <section class="week-team">
-         <h2>04-23: First drafts of Sobki</h2>
+         <h2>04-23: First drafts of Sobki, our mascot!</h2>
          <img src="https://static.igem.org/mediawiki/2017/0/02/T--INSA-UPS_France--Notebook_Week-16.jpg" alt="">
+      </section>
+      <section class="week-ihp">
+        <h1>04-25:</h1>
+        <p>
+          Following our appointment with Marc Lemonnier, we began to think about a device so that our system could treat the water in a tank for villagers or the water of a flask for the tourists. The challenge is to confine the microorganisms so that they do not go into the water.
+        </p>
        </section>
        <section class="week-meeting">
          <h2>04-26: 14th brainstorming</h2>
+        <p>
+          For safety reasons we can&rsquo;t use <i>V. cholerae</i> for our proof of concept, and we can&rsquo;t use any gene from that bacteria. As <i>V. harveyi</i> is genetically close to <i>V. cholerae</i> but not pathogenic for human, we can use it&rsquo;s quorum sensing molecule, the C8-CAI-1 that looks very much as the CAI-1, to mimic <i>V. cholerae</i>. Here, the idea is to engineer a bacteria to be a &ldquo;cholerae-like bacteria&rdquo; that produces a lot quorum sensing molecules to be detected by our detector <i>V. harveyi</i>.
+        </p>
+      </section>
+      <section class="week-team">
+        <h2>04-27:</h2>
+        <p>
+          T&eacute;o and Margaux went to a conference about <i>V. cholerae</i> to learn more about genetics of that bacteria, and the main differences between <i>V. cholerae</i> and <i>V. harveyi</i>.
+        </p>
        </section>
      </div>
@@ Line 1,072: / Line 1,063: @@
        <section class="week-meeting">
          <h2>05-02: 15th brainstorming</h2>
+        <p>
+          We need to evaluate the volume of water to treat. In the case of our device contain 1L water, it&rsquo;s for only 1 person but we can imagine to treat a high volume for an entire village. We&rsquo;ve prepared a list of questions to ask to NGOs to make a user friendly device.
+        </p>
+      </section>
+      <section class="week-pe">
+        <h2>05-07:</h2>
+        <p>
+          We&rsquo;ve met 2 game designers and graphists students, Vincent and Julien, to help us in the conception of our card game about microbiology! They will make the graphism designs of our card game.
+        </p>
        </section>
      </div>
@@ Line 1,083: / Line 1,083: @@
        <section class="week-meeting">
          <h2>05-11: 16th brainstorming</h2>
+        <p>
+          For the detection of diacetyl, we found the receptor Odr10, that activate the promoter pFUS for peptide production. For diacetyl production, the gene ALS is known to enhance diacetyl production, so we&rsquo;ll need to express ALS gene in <i>V. harveyi</i> only when C8-CAI-1 is detected. This is complicated because the receptor of C8-CAI-1 induces a repression of the promoter pqrr4. We need to find a inhibitor regulation in our system.
+        </p>
        </section>
        <section class="week-team">
@@ Line 1,098: / Line 1,101: @@
        <section class="week-meeting">
          <h2>05-17: 17th brainstorming</h2>
+        <p>
+           We planed to do NMR analysis to detect C8-CAI-1 and diacetyl for the proof of concept. We&rsquo;ve asked to Bassler&rsquo;s lab to give us 2 <i>V. harveyi</i> strains: a WT one, and the JMH626 strain deleted from the gene CqsA, which can&rsquo;t produce C8-CAI-1 by itself. We&rsquo;ll need that strain to detect C8-CAI-1 from our engineered &ldquo;<i>E. coli</i> cholera-like&rdquo;. Purified diacetyl has been commanded to do induction experiments on <i>P. pastoris</i>. We find the Tet on/ Tet off repression system for diacetyl production in response to C8-CAI-1.
+        </p>
        </section>
        <section class="week-experiment">
          <h2>05-18: iGEM distribution kit received</h2>
          <img src="https://static.igem.org/mediawiki/2017/7/70/T--INSA-UPS_France--Notebook_Week-20.jpg" alt="">
+      </section>
+      <section class="week-team">
+        <h2>05-21 Sobki is born!</h2>
+        <img src="" alt="">
        </section>
      </div>
@@ Line 1,109: / Line 1,119: @@
        <section>
          <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 21 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+      </section>
+      <section class="week-ihp">
+        <p>
+          We made a survey about cholera disease and about our project to know if people are aware of cholera epidemics in Africa and the existing solutions, and to know if people would be ready to use a device containing GMMs to treat the water they drink.
+        </p>
        </section>
        <section class="week-team">
@@ Line 1,128: / Line 1,143: @@
        </section>
        <section class="week-pe">
-         <h2>05-31: Exposciences</h2>
+         <h2>05-30 and 31: Exposciences</h2>
          <p>
-           We took part to the Exposciences event.
+           We took part to &ldquo;Exposciences&rdquo; which is a scientific festival. During this event we made children extract banana DNA thanks to simple ingredients that they can find in their kitchen. We also discussed with them about microorganisms thanks to paper fortune teller and this support led them to be curious on the microscopic world around them.
          </p>
-        <!-- LINK -->
          <img src="https://static.igem.org/mediawiki/2017/f/fb/T--INSA-UPS_France--Engagement-Exposciences_1.jpg" alt="">
          <h2>06-01: Interventions in schools</h2>
-         <!-- LINK -->
+         <p>
+          The main goals of these interventions were the discovery of biology and research at school with two workshops: Microorganisms and their environment and growth of microorganisms on a Petri dish.
+        </p>
          <img src="https://static.igem.org/mediawiki/2017/6/61/T--INSA-UPS_France--Notebook_Week-22.jpg" alt="">
        </section>
@@ Line 1,179: / Line 1,195: @@
        <section class="week-ihp">
          <h2>06-06: Meeting with the CEO of Sunwaterlife</h2>
+        <p>
+          We presented our project to Christophe Camp&eacute;ri-Ginestet who seems to be interested, especially by the detection function of <i>V. cholerae</i>. He would like to adapt it to the Sunwaterlife filters in order to have a direct colored response according to the water contamination. In addition, he would be willing to invest in our project in order to apply for JEI status (or &ldquo;Young Innovative Enterprises&rdquo; in english), a very interesting status for start-ups allowing them to have financial advantages. Besides, a collaboration is discussed so that Sunwaterlife provide us with their membranes, allowing us to confine our microorganisms within a device. He told us that he might be able to put us in touch with a UNICEF person in charge of cholera epidemics.
+        </p>
          <p>
            You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a4">his testimony here.</a>
@@ Line 1,185: / Line 1,204: @@
        <section class="week-meeting">
          <h2>06-08: 18th brainstorming</h2>
+        <p>
+          We&rsquo;ve divided the subject in 3 modules. The &ldquo;Quorum&rdquo; module, with <i>E. coli</i> engineered to produce C8-CAI-1; the &ldquo;Detection&rdquo; module, with V. harveyi engineered to detect C8-CAI-1 and produce diacetyl in response; and the &ldquo;Killing&rdquo; module with <i>P. pastoris</i> engineered to detect diacetyl and produce crocodile AMP in response.
+        </p>
        </section>
        <section class="week-pe">
          <h2>06-09: Intervention in school</h2>
+        <p>
+          The main goals of these interventions were the discovery of biology and research at school with two workshops: Microorganisms and their environment and growth of microorganisms on a Petri dish.
+        </p>
          <img src="https://static.igem.org/mediawiki/2017/6/60/T--INSA-UPS_France--Engagement_school1.JPG" alt="">
        </section>
@@ Line 1,228: / Line 1,253: @@
        <section>
          <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 24 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+      </section>
+      <section class="week-experiment">
+        <h2>06-13: Model</h2>
+        <p>
+          A first version of the SBGN (Systems Biology Graphical Notation) summarizing our synthetic system have been drawn.
+        </p>
        </section>
        <section class="week-ihp">
          <h2>06-14: Scope statement start</h2>
-         <p><i>
+         <p>
-           Third device proposition
+           A scope statement was created for a device that treats the water of a tank and another for a device that treats the water in a flask.
-         </i></p>
+         </p>
        </section>
        <section class="week-meeting">
          <h2>06-15: 19th brainstorming</h2>
+        <p>
+          We&rsquo;ve received our IDT parts! We finally choose the name of our project: Croc&rsquo;n cholera.
+        </p>
        </section>
        <section class="week-igemers">
@@ Line 1,266: / Line 1,300: @@
              </div>
              <div>
-               <!--<h2>Titre</h2>
+               <h2>Cloning</h2>
-               <p>
+               <ul>
-                 Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+                 <li>pPICz&alpha;, jmp62-DsRed (pDsRed) and jmp62-YFP (pYFP) were transformed into E.coli DHA&alpha; in order to amplify them.  </li>
-              </p>-->
+                <li>Bacteria grown were then put into liquid culture to do miniprep.</li>
+                <li>Digestion BamHI/EcoRI to check pPICz&alpha; gel migration profile<br />
+Digestion XbaI/NheI to check jmp62-DsRed gel migration profile<br />
+Digestion BamHI/NheI to check jmp62-YFP gel migration profile</li>
+              </ul>
              </div>
            </div>
@@ Line 1,283: / Line 1,321: @@
        <section class="week-ihp">
          <h2>06-19: Meeting with Doctors Without Borders, regional manager</h2>
+        <p>
+          She explained that the detection of cholera in the patient is simple because the symptoms are known. She told us that our system would be more useful treating water tanks in villages far from cholera camps rather than tourists. Indeed, governments often hide epidemics from tourists and tourist places take enough precautions to avoid tourists&rsquo; contamination.
+        </p>
          <p>
            You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a5">her testimony here.</a>
          </p>
-         <h2>06-20: Cholera survey</h2>
+         <h2>06-23: Beginning of the marketing approach</h2>
          <p>
-           We shared our survey about cholera on the social media.
+           A marketing approach has been initiated to study the strengths and weaknesses of our two potential products and to determine the approach to sell them. Thanks to a SWOT analysis on both products, we realized that the product to treat water in a flask is not adapted to the market. This product is therefore abandoned and we focus on a product that processes a larger volume of water, suitable for villagers in the affected countries.
-          <!-- lINK -->
          </p>
        </section>
@@ Line 1,296: / Line 1,336: @@
        </section>
        <section class="week-meeting">
-         <h2>06-23: Meeting with all the team members and supervisors</h2>
+         <h2>06-23: 20th brainstorming</h2>
-         <p><i>
+         <p>
-           Final choice of the global device purpose
+           Meeting with all the team members and supervisors and final choice of the global device purpose: made for people living in remote areas in Africa, which are far from NGOs camps and more vulnerable to a cholera epidemy.
-         </i></p>
+         </p>
+      </section>
+      <section class="week-experiment">
+        <h2>06-23: Model</h2>
+        <p>
+          Our mathematical model based on twelve ODEs was built!
+        </p>
        </section>
        <section class="week-team">
@@ Line 1,331: / Line 1,377: @@
              </div>
              <div>
-               <!--<h2>Titre</h2>
+               <h2>Cloning</h2>
-               <p>
+               <ul>
-                 Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+                 <li>Preparative digestion of pPICZ&alpha; with BamHI/BglII to clone our gene followed by a gel extraction to purify the open plasmid.</li>
-               </p>-->
+                <li>Glycerol stocks of <i>E.coli</i> DHA&alpha; containing pPICZ&alpha;, pDSRed and pYFP were made after liquid culture. </li>
+               </ul>
              </div>
            </div>
@@ Line 1,351: / Line 1,398: @@
        <section class="week-ihp">
          <h2>06-28: Meeting with the CEO of Sunwaterlife, building a collaboration</h2>
+        <p>
+          Really interested in the ability to detect <i>V. cholerae</i> in water, Christophe Camp&eacute;ri-Ginestet asked us to provide our bibliographic research and results at the end of the iGEM project. In addition, in exchange for our financial investment in our project, we have to fill out the JEI document which attests to our R&amp;D activity for Sunwaterlife. He also put us in touch with Alama Keita, cholera leader of UNICEF in Niger. Finally, we talked to him about our product to contain our microorganisms. He helped us to design a cylindrical device whose sections would be these membranes to ensure GMMs containment.
+        </p>
          <p>
            You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a4">his testimony here.</a>
          </p>
          <h2>06-30: First steps of our ethical matrix</h2>
-         <!-- img éthique -->
+         <img src="https://static.igem.org/mediawiki/2017/6/6b/T--INSA-UPS_France--Notebook_Week-26.jpg" alt="">
        </section>
        <section class="week-meeting">
          <h2>06-29: 21th brainstorming</h2>
-      </section>
+        <p>
-      <section class="week-team">
+          We choose to made a Prezi for the presentation in Boston, the structure has been validated by instructors and students, and we made a first version of our poster for the meetup in Delft. Our wiki is online next day! Our survey has around 400 answers, so we can start analyses of results.
-        <h2>06-30: Tropicool!</h2>
+         </p>
-         <img src="https://static.igem.org/mediawiki/2017/6/6b/T--INSA-UPS_France--Notebook_Week-26.jpg" alt="">
        </section>
        <section class="week-experiment">
@@ Line 1,389: / Line 1,438: @@
              </div>
              <div>
-               <!--<h2>Titre</h2>
+               <h2>Cloning</h2>
-               <p>
+               <ul>
-                 Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+                 <li>PCR of DsRed and YFP with primers containing restriction sites. PCR products for these genes have right size. PCR products are purified.</li>
-              </p>-->
+                <li>PCR of our parts (cOT2 ; leucrocine ; DNY15; Odr10) from IDT GBlocks. All PCRs succeeded.</li>
+                <li>cOT2&nbsp;; leucrocine&nbsp;; DNY15 ; Odr10 were digested BamHI/BglII to be inserted in pPICZ&alpha;.</li>
+                <li>Transformation of pPICZ&alpha;-cOT2 ; pPICZ&alpha;-leucrocine ; pPICZ&alpha;-Odr10 and pPICZ&alpha;-DNY15 in <i>E. coli</i> DH5&alpha;</li>
+                <li>Miniprep of liquid culture transformants</li>
+                <li>Digestion BmaHI/BglII to check the gel migration profile. <br />&rarr; It appears that the plasmid was well digested but the insert does not appear. There is an intense band around 1300bp that might correspond to supercoiled plasmid. To conclude it seems like pPICZ&alpha; closed itself without the insert: BamHI and BglII are compatibles.
+                </li>
+                <li>New primers were ordered replace by PCR the BglII site by KpnI site to remove the possibility for the plasmid to close itself.</li>
+              </ul>
              </div>
            </div>
@@ Line 1,406: / Line 1,462: @@
        <section class="week-meeting">
          <h2>07-06: 22th brainstorming</h2>
+        <p>
+          We&rsquo;ve prepared some public engagement events for september : an exhibition at the campus science in Toulouse to showcase ancient iGEM Toulouse projects, and synthetic biology, a practical work in an high school, and we&rsquo;ve planned to participate to the &ldquo;European Researcher&rsquo;s Night&rdquo; with the LISBP by creating a game called &ldquo;Possible or Impossible&rdquo;, to open a dialogue about GMO legislation with people.
+        </p>
        </section>
        <section class="week-igemers">
@@ Line 1,436: / Line 1,495: @@
              </div>
              <div>
-               <!--<h2>Titre</h2>
+               <h2>Cloning</h2>
-               <p>
+               <ul>
-                 Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+                 <li>While waiting for the new primers, another try was made using for the ligation a 1:10 ratio (pPICZ&alpha;:insert).</li>
-              </p>-->
+                <li>Transformants appeared in all plates except for Odr10.</li>
+                <li>Miniprep and digestions of transformants to check the gel migration profile.</li>
+                <li>We obtained 2 clones pPICZ&alpha;-DNY15. The gel confirmed that D1 and D2 are clones with the entire plasmid pPICZa A open only in BamHI site with one insert in this site. D1 has the insert in the good direction and D2 has the insert in the opposite direction.</li>
+                <li>Clones D1 and D2 were stored in glycerol.</li>
+                <li>At the end of the week, PCR with new primers containing KpnI instead of BglII were made for all the parts (cOT2 ; leucrocine ; DNY15 ; Odr10). All the PCR worked perfectly, PCR tubes were pooled. Odr10 has secondary products which need gel extraction. </li>
+                <li>Digestion of plasmid and parts with KpnI and BamHI</li>
+              </ul>
              </div>
            </div>
@@ Line 1,453: / Line 1,518: @@
        <section class="week-meeting">
          <h2>07-13: 23th brainstorming</h2>
+        <p>
+           Citation from C&eacute;dric : &ldquo;we must be humble before molecular biology&rdquo;. Our card game conception progressing well! We find a name : MicrobioWorld, and the main rules has been determined after several crazy gameplay!
+        </p>
        </section>
        <section class="week-ihp">
          <h2>07-14: Meeting with Doctors Without Borders, cholera specialist</h2>
+        <p>
+          Fransisco Luquero assured us that the current solutions (bleach, boil water) were not the most suitable to treat water from cholera. Our device could be interesting if it is suitable to treat several liters of water and even if it contains GMM, it could be easily accepted by the population as long as it does not change the color and taste of water.
+        </p>
        </section>
        <section class="week-experiment">
@@ Line 1,480: / Line 1,551: @@
              </div>
              <div>
-               <!--<h2>Titre</h2>
+               <h2>Cloning</h2>
-               <p>
+              <ul>
-                 Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+                <li>Ligation and transformation of pPICZ&alpha;-cOT2 ; pPICZ&alpha;-leucrocine ; pPICZ&alpha;-Odr10 and pPICZ&alpha;-DNY15.</li>
-              </p>-->
+                <li>Miniprep of transformants and gel migration were made. The ladder was clear but there was no DNA on tranformants colums.</li>
+                <li>Nanodrop analysis showed that there was not enough DNA.<br />&rarr; The hypothesis is that the liquid culture was too long so <i>E. coli</i> lost the plasmid.</li>
+              </ul>
+              <h2>Experiments with <i>P. pastoris</i></h2>
+               <ul>
+                 <li>We did plates of YPD with increasing concentration of zeocin to select <i>P. pastoris</i> clones which integrated the most copies of the construction.</li>
+                <li>Preparation of competent yeast (<i>Pichia pastoris</i>)</li>
+                <li>Digestion of D1 clone (pPICZ&alpha;-DNY15) with HindIII to integrate the plasmid construction at the AOX1 promotor in <I>P. pastoris</I> genome.</li>
+                <li>Electroporation of competent yeast with the digested DNA.</li>
+              </ul>
              </div>
            </div>
@@ Line 1,494: / Line 1,574: @@
        <section>
          <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 29 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+      </section>
+      <section class="week-experiment">
+        <h2>07-17: Model</h2>
+        <p>
+          Our system of ODEs script and function have been developed in Matlab.
+        </p>
        </section>
        <section class="week-meeting">
          <h2>07-20: 24th brainstorming</h2>
+        <p>
+          We&rsquo;ve finished a first version of our presentation, which will be presented during the Parisian Meetup. The Matlab programming is done, the next step is to add values from bibliography.
+        </p>
+      </section>
+      <section class="week-ihp">
+        <h2>07-20: First meeting with Pierre-Alain Hoffmann</h2>
+        <p>
+          We met Pierre-Alain Hoffmann, Deputy Director of CRITT Bio-Industries for the business plan elaboration. The knowledge in creation and development of companies of Pierre-Alain Hoffmann allowed us to understand the goal of a business plan. He accompanied us in this approach, explaining the points to be detailed in order to better integrate our system in the market.
+        </p>
        </section>
        <section class="week-igemers">
@@ Line 1,527: / Line 1,622: @@
              </div>
              <div>
-               <!--<h2>Titre</h2>
+               <h2>Cloning</h2>
-               <p>
+              <ul>
-                 Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+                <li>This time transformation were made with <i>E. coli</i> XL1 Blue which are supposed to be more efficient.</li>
-               </p>-->
+                <li>No DNA after gel migration like the previous week.</li>
+              </ul>
+              <h2>Experiments with <i>P. pastoris</i></h2>
+               <ul>
+                 <li>Colony PCR of <i>P. pastoris</i> clone which grew on YPD zeocin. &rarr; All clones have the gene construction in their genome!</li>
+                <li>The 11 clones of Pichia were grown on plates with 250 &micro;g/mL, 500 &micro;g/mL and 1000 &micro;g/mL to see which one has the best integration of the construction. All clones grew onto 250, 500 and 1000 &micro;g/mL zeocin.</li>
+               </ul>
              </div>
            </div>
@@ Line 1,547: / Line 1,648: @@
        </section>
        <section class="week-ihp">
-         <h2>07-27: Meeting at TWB</h2>
+         <h2>07-27: Meeting with Pierre Monsan at TWB</h2>
+        <p>
+          As a preindustrial demonstrator recognized in France, TWB helps many projects to become companies. We presented them our project and had a positive feedback. It is even one of our biggest sponsors.
+        </p>
          <p>
            You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a2">their testimony here.</a>
@@ Line 1,580: / Line 1,684: @@
              </div>
              <div>
-               <!--<h2>Titre</h2>
+               <h2>Cloning</h2>
-               <p>
+              <ul>
-                 Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+                <li>PCR were made again of all parts in order to have enough materials to do ligation.</li>
-              </p>-->
+                <li>This time lot of DNA was digested.</li>
+                <li>Several strategies were tried to obtain pPICZ&alpha;-cOT2 ; pPICZ&alpha;-leucrocine &nbsp;and pPICZ&alpha;-Odr10 with DHA&alpha; and XL1 Blue strains.</li>
+                <li>At the end of the week we got 2 clones of pPICZ&alpha;-Odr10 in E. coli DHA&alpha; </li>
+              </ul>
+              <h2>Experiments with <i>P. pastoris</i></h2>
+               <ul>
+                 <li>The supernatant of <i>P. pastoris</i> containing the DNY15 gene was extracted and then concentrated via rotavapor and freeze-drying.</li>
+                <li>In a 24 wells plate, kinetic growths of <i>V. harveyi</i> were followed with or without <i>P. pastoris</i> supernatant to check the bactericide effect of DNY15. &rarr; this first try was not perfectly made</li>
+                <li>Inhibition test with the same supernatant was made on petri dish. &rarr; this first try was not perfectly made</li>
+              </ul>
              </div>
            </div>
@@ Line 1,597: / Line 1,710: @@
        <section class="week-meeting">
          <h2>08-01: 25th brainstorming</h2>
+        <p>
+          Our collaborations with other iGEM teams are in process! We’ve started an experiment for iGEM Purdue.
+        </p>
        </section>
        <section class="week-experiment">
@@ Line 1,623: / Line 1,739: @@
              </div>
              <div>
-               <!--<h2>Titre</h2>
+               <h2>Cloning</h2>
-               <p>
+              <ul>
-                 Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+                <li>Different strategies were made again to obtain pPICZ&alpha;-cOT2 and pPICZ&alpha;-leucrocine in E.coli. &rarr; Several clones of pPICZ&alpha;-cOT2 and pPICZ&alpha;-leucrocine were obtained!!!</li>
-              </p>-->
+                <li>Liquid culture of DHA&alpha; containing pSB1C3 was made to get enough material to do cloning of our parts in it.</li>
+                <li>Ligation of YFP in pPICZ&alpha;-Odr10 and in pPICZ&alpha;-DNY15 instead of the AMP gene.</li>
+                <li>Integration of AMP genes in pSBIC3 (=iGEM parts).</li>
+              </ul>
+              <h2>Experiments with <i>P. pastoris</i></h2>
+               <ul>
+                 <li>Cytotoxicity test of DNY15 was tried again with kinetic growths of <i>V. harveyi</i>.</li>
+                <li>Cytotoxicity test of DNY15 was tried again on petri dish: No effect of DNY15 can be seen. </li>
+                <li>Genomic integration of pPICZ&alpha;-cOT2 , pPICZ&alpha;-leucrocine and pPICZ&alpha;-Odr10.</li>
+              </ul>
              </div>
            </div>
@@ Line 1,641: / Line 1,766: @@
        <section class="week-igemers">
          <h2>08-07: Skype with the iGEM team of Boston</h2>
+      </section>
+      <section class="week-experiment">
+        <h2>08-07: Model</h2>
+        <p>
+          All the experimental and literature values have been implemented in our Matlab script, which provides promising results. These first results will be used for preliminary work in design and entrepreneurship.
+        </p>
        </section>
        <section class="week-experiment">
@@ Line 1,666: / Line 1,797: @@
              </div>
              <div>
-               <!--<h2>Titre</h2>
+               <h2>Cloning</h2>
                <p>
-                 Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+                 We obtained the YFP gene after the GAP promotor and the FUS1 promotor in <i>E. coli</i>.
-               </p>-->
+               </p>
+              <h2>Experiments with <i>P. pastoris</i></h2>
+              <ul>
+                <li>pGAP-YFP and pFUS-YFP were transformed in <i>P. pastoris</i>.</li>
+                <li><i>P. pastoris</i> clones were obtained with pPICZ&alpha;-Odr10 ; pPICZ&alpha;-leucrocine and pPICZ&alpha;-cOT2 but the PCR colony didn&rsquo;t work. After several trials and controls we concluded that the DNA extraction the way we did it was not enough to do a PCR of the GAP promotor in the pichia genome.</li>
+                <li>A kinetic study of <i>V. harveyi</i> growth was made using pichia supernatant. &rarr; A difference was observed but the experiment needs to be done again.</li>
+              </ul>
              </div>
            </div>
@@ Line 1,713: / Line 1,850: @@
              </div>
              <div>
-               <!--<h2>Titre</h2>
+               <ul>
-              <p>
+                <li><i>P. pastoris</i> clones were obtained with the YFP reporter behind both promotor: GAP and FUS1.</li>
-                 Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+                <li>Fluorescence test was made but it didn’t work.</li>
-               </p>-->
+                 <li>A cytotoxicity test was made on V.harveyi with pichia-AMP supernantant but we had trouble because of volumes used.</li>
+                <li>We tried a new DNA extraction protocol to do the PCR colony on <i>P. pastoris</i> and it did work.</li>
+               </ul>
              </div>
            </div>
@@ Line 1,731: / Line 1,870: @@
        <section class="week-meeting">
          <h2>08-24: 26th brainstorming</h2>
+        <p>
+          We finally choose our track! We will be candidate in &ldquo;information processing&rdquo;, to highlight the prokaryote-eukaryote communications in our multimicrobial system!
+        </p>
        </section>
        <section class="week-experiment">
@@ Line 1,758: / Line 1,900: @@
              </div>
              <div>
-               <!--<h2>Titre</h2>
+               <ul>
-              <p>
+                <li>Cytotoxicity test was made again but this time on a petri dish. We had a halo for the antibiotic but <i>P. pastoris&rsquo;</i> supernatant didn&rsquo;t stop <i>V. harveyi&rsquo;</i>s growth.</li>
-                 Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+                 <li>We&rsquo;ve tried to characterize pFUS1 promoter in different media but we got no positive result.</li>
-               </p>-->
+               </ul>
              </div>
            </div>
@@ Line 1,772: / Line 1,914: @@
        <section>
          <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 35 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+      </section>
+      <section class="week-ihp">
+        <h2>08-28: First try for the device modeling</h2>
+        <p>
+           A 3D modeling of the device was initiated on the Solidworks software with the help of a professional in the design of industrial parts. The objective is to model the device in 3D and then to print the prototype.
+        </p>
        </section>
        <section class="week-meeting">
          <h2>08-31: 27th brainstorming</h2>
+        <p>
+          After much thinking, we are agreed that we want Margaux, Maxant and Margaux to be our speakers during the competition in Boston!
+        </p>
+      </section>
+      <section class="week-experiment">
+        <h2> 08-31: Model</h2>
+        <p>
+          First MCA results! The control exerted by each parameter on the response time can now be analysed.
+        </p>
        </section>
        <section class="week-experiment">
@@ Line 1,799: / Line 1,956: @@
              </div>
              <div>
-               <!--<h2>Titre</h2>
+               <ul>
-              <p>
+                <li>This time, we tested the production of DNY15 in YPD with 40g/L of glucose instead of 30g/L because publications which use pGAP as a promotor to produce AMP sometimes have a higher production with more glucose.</li>
-                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+                <li>Cytotoxicity tests using the supernatant from the 40g/L glucose culture media was made. Supernatants were filtered beforehand and half was freeze-dried. &nbsp;We got a weak halo for the DNY15 production in glucose 40 and supernatant freeze-dried!</li>
-               </p>-->
+               </ul>
              </div>
            </div>
@@ Line 1,849: / Line 2,006: @@
              </div>
              <div>
-               <!--<h2>Titre</h2>
+               <ul>
-              <p>
+                <li>Because we still had trouble integrating some parts in <i>P. pastoris&rsquo;</i> genome we tried the integration via electroporation and via chemical way at the same time.</li>
-                 Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+                 <li>We launched one again <i>P. pastoris</i> culture in YPD 40g/L and 50g/L glucose to try producing more DNY15.</li>
-               </p>-->
+               </ul>
              </div>
            </div>
@@ Line 1,866: / Line 2,023: @@
        <section class="week-meeting">
          <h2>09-14: 29th brainstorming</h2>
+        <p>
+          We&rsquo;ve prepared the abstract, and choose a subtitle : &ldquo;a synthetic microbial consortium&rdquo;
+        </p>
        </section>
        <section class="week-experiment">
@@ Line 1,891: / Line 2,051: @@
              </div>
              <div>
-               <!--<h2>Titre</h2>
+               <ul>
-              <p>
+                <li>We prepared sample to sequence the AMP genes we wanted to give to the iGEM registry.</li>
-                Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+                <li>Culture media from <i>P. pastoris</i> culture of last week were freeze-dried. And the cytotoxicity test on petri dish was made again but this time, even the positive control with antibiotic didn&rsquo;t work.</li>
-              </p>-->
+                <li><i>P. pastoris</i> clones were obtained with pAOX1-leucrocine, pAOX1-cOT2, Odr10-pFUS-RFP.</li>
+              </ul>
              </div>
            </div>
@@ Line 1,908: / Line 2,069: @@
        <section class="week-meeting">
          <h2>09-21: 30th brainstorming</h2>
+        <p>
+          We&rsquo;ve finished the experiment time, it&rsquo;s time to write results for the wiki!
+        </p>
        </section>
        <section class="week-ihp">
          <h2>09-22: Meeting with UNICEF</h2>
+        <p>
+          He warned us that NGOs would agree to use a GMM system only if efficacy is proven and there is no danger of GMM proliferation. He also gave us an idea of the volume of water a village needs. Moreover, it is also important to make a system with as little waste as possible.
+        </p>
          <p>
            You can read the result of <a href="https://2017.igem.org/Team:INSA-UPS_France/Entrepreneurship/Testimonies#a6">Alama Keita's testimony here.</a>
@@ Line 1,939: / Line 2,106: @@
              </div>
              <div>
-               <!--<h2>Titre</h2>
+               <ul>
-              <p>
+                <li>AMP genes were sent to be sequenced.</li>
-                 Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+                <li><i>P. pastoris</i> producing DNY15 was cultured to do a RT-qPCR experiment to compare it to the WT <i>P. pastoris</i>.</li>
-               </p>-->
+                <li>New cytotoxicity tests on petri dish were made but this time it didn&rsquo;t work because of the paper disk we used. Once again the positive control didn&rsquo;t work.</li>
+                 <li>Cultures in BMMY adding methanol every 24h for 120h were made for both pAOX1-leucrocine and pAOX1-cOT2 P.pastoris clones. Half of each supernatant was stored at 4&deg;C and the other half was evaporated to concentrate 10 times.</li>
+               </ul>
              </div>
            </div>
@@ Line 1,956: / Line 2,125: @@
        <section class="week-meeting">
          <h2>09-27: 31th brainstorming</h2>
+        <p>
+          We&rsquo;ve started to make rehearsals for the presentation in Boston.
+        </p>
        </section>
        <section class="week-pe">
          <h2>09-29: European Researcher's Night</h2>
+        <p>
+          We&rsquo;ve been to the European Researcher&rsquo;s Night, a major scientific event that gather researchers and general public in a convivial atmosphere. In 2017, the topic of the event was &ldquo;Impossible&rdquo;. In order to follow this thematic, we chose to focus our workshop on the incredible features of biodiversity, how to use it in synthetic biology, and what are legal limits on GMO use in France.
+        </p>
          <img src="https://static.igem.org/mediawiki/2017/0/05/T--INSA-UPS_France--Notebook_Week-39.jpg" alt="">
          <!-- link -->
@@ Line 1,984: / Line 2,159: @@
              </div>
              <div>
-               <!--<h2>Titre</h2>
+               <ul>
-              <p>
+                <li>New cytotoxicity tests on petri dishes were made with the leucrocine and cOT2 supernatants. This time with appropriate discs. Positive control did not work again.</li>
-                 Lorem ipsum dolor sit amet, consectetur adipisicing elit. Odio inventore modi dignissimos sint. Nobis nulla eum soluta vero quibusdam maxime, quo totam non, at provident labore. Ut dolorum libero totam!
+                <li> New cytotoxicity tests on petri dishes were made but using pure chloramphenicol. A weak halo was seen. We found out that the <i>V. harveyi</i> strain we used to do cytotoxicity test was maybe! the JMH626 one which was chloramphenicol-resistant. </li>
-               </p>-->
+                <li>RT-PCR experiment was made.</li>
+                <li>To test the Odr10-RFP system and activate pFUS, nitrogen depletion cultures were done and two sources of nitrogen were tested: sulfate ammonium and glutamine. The fluorescence following was made in a plate reader.</li>
+                 <li>New cytotoxicity tests on petri dishes were made starting from a fresh <i>V. harveyi</i> cuture. Leucrocine, cOT2 and D-NY15 (40g/L and 50g/L glucose) productions were tested. Halos are clearly visible for D-NY15 40g / L not concentrated and concentrated 10 times.</li>
+                <li>A 24 well plate was launched by adding leucrocine, cOT2 and D-NY15 supernatant to <i>V. harveyi</i> cultures in exponential phase.</li>
+               </ul>
              </div>
            </div>
@@ Line 1,998: / Line 2,177: @@
        <section>
          <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 40 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+      </section>
+      <section class="week-ihp">
+        <h2>10-02: Meeting with Pierre-Alain Hoffmann, director of CRITT Bio-industries.</h2>
+        <p>
+          Between these two appointments, we have elaborated the business plan ourselves. Pierre-Alain Hoffmann read it and made a feed-back. He was pleased with our work, ensuring that some start-ups do not start their business with such a business plan.
+        </p>
        </section>
        <section class="week-meeting">
-         <h2>09-05: 32th brainstorming</h2>
+         <h2>10-05: 32th brainstorming</h2>
+        <p>
+           Rehearsals, and writing of different pages of the wiki.
+        </p>
        </section>
        <section class="week-pe">
-         <h2>09-04: High school practical work</h2>
+         <h2>10-04: High school practical work</h2>
+        <p>
+          We prepared an intervention for High School senior students in scientific classes. Our involvement was focused on 3 main goals: implement a learner-centred pedagogical approach in a high school class, make discover biotechnologies through a practical approach, and discuss on &ldquo;how to be part of science, and which paths to choose after high school?&rdquo;.
+        </p>
          <img src="https://static.igem.org/mediawiki/2017/8/8a/T--INSA-UPS_France--Notebook_Week-42.jpg" alt="">
        </section>
@@ Line 2,012: / Line 2,203: @@
        <section>
          <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 41 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+      </section>
+      <section class="week-meeting">
+        <h2>33th brainstorming</h2>
+        <p>
+          Rehearsals, and writing of different pages of the wiki.
+        </p>
+      </section>
+      <section class="week-pe">
+        <h2>All week: exhibition at Bib'INSA</h2>
+        <p>
+          In order to make former iGEM Toulouse projects understandable for all scientist student (and not only for biologists), we redesigned posters of previous project E. calculus, SubtiTree, ApiColi, Paleotilis, and of our project Croc’n Cholera.
+        </p>
        </section>
@@ Line 2,020: / Line 2,223: @@
        <section>
          <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 42 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+      </section>
+      <section class="week-ihp">
+        <h2>10-10: End of the 3D modeling of the device and 3D printing</h2>
+        <p>
+          The device was printed in 3D by the High school Georges Cabanis at Brive-La-Gaillarde.
+        </p>
+        <img src="" alt="">
+      </section>
+      <section class="week-experiment">
+        <h2>10-11: Model</h2>
+        <p>
+          Some inaccuracies in our mathematical model have been corrected, we have now the final version of all our Matlab files! Results will be used for our entrepreneurship and design work.
+        </p>
+        <h2>10-13</h2>
+        <p>
+          We have now our final MCA, and performed a global analysis of our system. We can now definitely conclude about sensitivity and robustness of our model.
+        </p>
+      </section>
+      <section class="week-pe">
+        <h2>13-10: Seminar at the campus science Toulouse</h2>
+        <p>
+          After working during several months on synthetic biology for our iGEM project, we needed to organize an event to present our work and the one achieved by the previous iGEM teams. We also wanted to have an exchange of opinions with people who possibly have different values and considerations than us. The intended public was mainly composed of the Paul Sabatier university and INSA Toulouse students, two of the schools we are intending.
+        </p>
        </section>
      </div>
@@ Line 2,027: / Line 2,253: @@
        <section>
          <h1><i class="fa fa-chevron-left last-week" aria-hidden="true"></i> Week 43 <i class="fa fa-chevron-right next-week" aria-hidden="true"></i></h1>
+      </section>
+      <section class="week-experiment">
+        <h2>10-21: Model</h2>
+        <p>
+          An intuitive modeling interface have been built for displaying our model results to non-specialists.
+        </p>
        </section>
      </div>
@@ Line 2,038: / Line 2,270: @@
      <!-- fin section -->
+    <style>
+      .links_end{
+        background: none;
+      }
+      .links_end table{
+        width:100%;
+      }
+      .links_end th, .links_end td{
+        font-size:18pt;
+        padding:10px;
+      }
+      .links_end td{
+        border:solid #eee 1px;
+      }
+    </style>
+    <section class="links_end">
+      <table>
+        <tr>
+          <th colspan="6">
+            Realisations pages
+          </th>
+        </tr>
+        <tr>
+          <td><a href="https://2017.igem.org/Team:INSA-UPS_France/Experiments">Overview</a></td>
+          <td><i>Notebook</i></td>
+          <td><a href="https://2017.igem.org/Team:INSA-UPS_France/Experiments/Clonings">Clonings</a></td>
+          <td><a href="https://2017.igem.org/Team:INSA-UPS_France/Results">Results</a></td>
+<td><a href="https://2017.igem.org/Team:INSA-UPS_France/Contribution">Contribution</a></td>
+          <td><a href="https://2017.igem.org/Team:INSA-UPS_France/Protocols">Protocols</a></td>
+          <td><a href="https://2017.igem.org/Team:INSA-UPS_France/Safety">Safety</a></td>
+        </tr>
+      </table>
+    </section>
    </div>
 </div>
-  <style>
-footer{
-  position:relative;
-  text-align:center;
-  margin-top:20px;
-  background: #323537;
-  width:100%;
-}
-/* CONTACT ICONS */
-.icons{
-  display:inline-block;
-  margin:40px 0;
-}
-.icons > a{
-  color:black;
-  margin:10px;
-  text-shadow:2px 2px 0px white;
-}
-#fbIcon:hover{
-  color:#3b5998;
-  text-shadow:2px 2px 0 #000000;
-}
-#twitterIcon:hover{
-  color:#55acee;
-  text-shadow:2px 2px 0 #000000;
-}
-#contactIcon:hover{
-  color:#e5e5e5;
-  text-shadow:2px 2px 0 #000000;
-}
-#instaIcon:hover{
-  color:#8a3ab9;
-  text-shadow:2px 2px 0 #000000;
-}
-/* SPONSORS IMG */
-.footer_sponsors img{
-  max-height:50px;
-  display:inline-block;
-  margin:10px;
-  opacity:0.5;
-}
-.footer_sponsors img:hover{
-  opacity:1;
-}
-</style>
    <footer>
@@ Line 2,116: / Line 2,325: @@
        <a href="https://www.veolia.com/en"><img src="https://static.igem.org/mediawiki/2017/9/91/T--INSA-UPS_France--Logo_veolia.png" alt=""></a>
        <a href="https://www.france-science.org/-Homepage-English-.html"><img src="https://static.igem.org/mediawiki/2017/1/1a/T--INSA-UPS_France--Logo_ambassade.jpg" alt=""></a>
+      <a href="https://www-lbme.biotoul.fr/"><img src="https://static.igem.org/mediawiki/2017/5/51/T--INSA-UPS_France--Logo_LBME.png" alt=""></a>
+      <a href="https://www6.toulouse.inra.fr/metatoul_eng/"><img src="https://static.igem.org/mediawiki/2017/1/16/T--INSA-UPS_France--Logo_metatoul.png" alt=""></a>
        <a href="http://www.univ-tlse3.fr/associations-+/do-you-have-a-project--378066.kjsp?RH=1238417866394"><img src="https://static.igem.org/mediawiki/2017/5/5b/T--INSA-UPS_France--Logo_fsdie.png" alt=""></a>
        <a href="http://en.univ-toulouse.fr/our-strengths"><img src="https://static.igem.org/mediawiki/2017/9/93/T--INSA-UPS_France--Logo_fsie.jpg" alt=""></a>

26	27	28	1	2	3	4
5	6	7	8	9	10	11
12	13	14	15	16	17	18
19	20	21	22	23	24	25
26	27	28	29	30	31	1

26	27	28	29	30	31	1
2	3	4	5	6	7	8
9	10	11	12	13	14	15
16	17	18	19	20	21	22
23	24	25	26	27	28	29
30	1	2	3	4	5	6

28	29	30	31	1	2	3
4	5	6	7	8	9	10
11	12	13	14	15	16	17
18	19	20	21	22	23	24
25	26	27	28	29	30	1

25	26	27	28	29	30	1
2	3	4	5	6	7	8
9	10	11	12	13	14	15
16	17	18	19	20	21	22
23	24	25	26	27	28	29
30	31	1	2	3	4	5

27	28	29	30	31	1	2
3	4	5	6	7	8	9
10	11	12	13	14	15	16
17	18	19	20	21	22	23
24	25	26	27	28	29	30

26	27	28	1	2	3	4
5	6	7	8	9	10	11
12	13	14	15	16	17	18
19	20	21	22	23	24	25
26	27	28	29	30	31	1

26	27	28	29	30	31	1
2	3	4	5	6	7	8
9	10	11	12	13	14	15
16	17	18	19	20	21	22
23	24	25	26	27	28	29
30	1	2	3	4	5	6

28	29	30	31	1	2	3
4	5	6	7	8	9	10
11	12	13	14	15	16	17
18	19	20	21	22	23	24
25	26	27	28	29	30	1

25	26	27	28	29	30	1
2	3	4	5	6	7	8
9	10	11	12	13	14	15
16	17	18	19	20	21	22
23	24	25	26	27	28	29
30	31	1	2	3	4	5

27	28	29	30	31	1	2
3	4	5	6	7	8	9
10	11	12	13	14	15	16
17	18	19	20	21	22	23
24	25	26	27	28	29	30

Difference between revisions of "Team:INSA-UPS France/Notebook"

Latest revision as of 22:20, 1 November 2017

Notebook

January

February

March

April

May

June

July

August

September

October

Week 1

Week 2

Week 3

Week 4

01-25: Kick Off Meeting

Week 5

01-30: 2th brainstorming: on the track of crocodile antimicrobial peptides...

Week 6

02-06: 3th brainstorming meeting

Week 7

02-16: 4th brainstorming

02-13 to 02-17: Training course for INSA students at LISBP with our instructor Brice Enjalbert

Week 8

02-23: 5th brainstorming

Week 9

03-02: 6th brainstorming

Week 10

03-05: Team building at the restaurant

03-09: 7th brainstorming meeting

Week 11

03-12: Brainstorming in a café

03-14: 8th brainstorming

Week 12

03-21: 9th brainstorming

Week 13

03-26: Brainstorming in a café

03-30: 10th brainstorming

Week 14

04-06: 11th brainstorming

04-07: choice of the subject

Week 15

04-13: 12th brainstorming

04-10 to 04-14 Training course for the UPS students at LBME with our instructors Anthony Henras and Yves Romeo

Week 16

04-19: Meeting with Marc Lemonnier, CEO of Antabio

04-20: 13th brainstorming

Week 17

04-23: First drafts of Sobki, our mascot!

04-25:

04-26: 14th brainstorming

04-27:

Week 18

05-02: 15th brainstorming

05-07:

Week 19

05-11: 16th brainstorming

05-13: Paul & Margaux officialy joined our team!

Week 20

05-17: 17th brainstorming

05-18: iGEM distribution kit received

05-21 Sobki is born!

Week 21

05-25: Parts design day at Brice's

Week 22

05-28: Official announcement of our subject

05-30 and 31: Exposciences

06-01: Interventions in schools

Experiments

Design and orders

Design and orders

Week 23

06-06: Meeting with the CEO of Sunwaterlife

06-08: 18th brainstorming

06-09: Intervention in school

Experiments

Taking care of V. harveyi

Week 24

Experiments with P. pastoris

26	27	28	1	2	3	4
5	6	7	8	9	10	11
12	13	14	15	16	17	18
19	20	21	22	23	24	25
26	27	28	29	30	31	1

26	27	28	29	30	31	1
2	3	4	5	6	7	8
9	10	11	12	13	14	15
16	17	18	19	20	21	22
23	24	25	26	27	28	29
30	1	2	3	4	5	6

28	29	30	31	1	2	3
4	5	6	7	8	9	10
11	12	13	14	15	16	17
18	19	20	21	22	23	24
25	26	27	28	29	30	1

25	26	27	28	29	30	1
2	3	4	5	6	7	8
9	10	11	12	13	14	15
16	17	18	19	20	21	22
23	24	25	26	27	28	29
30	31	1	2	3	4	5

27	28	29	30	31	1	2
3	4	5	6	7	8	9
10	11	12	13	14	15	16
17	18	19	20	21	22	23
24	25	26	27	28	29	30