Difference between revisions of "Team:Kyoto/Basic Part"

(Prototype team page)
 
 
(23 intermediate revisions by 2 users not shown)
Line 1: Line 1:
 
{{Kyoto}}
 
{{Kyoto}}
 +
{{Kyoto/header}}
 
<html>
 
<html>
 +
<style type="text/css">
 +
  ul.result {
 +
    margin-left:10%;
 +
    list-style:none;
 +
    text-align:left;
 +
    font-family: serif, 'Times New Roman';
 +
    font-size:150%;
 +
  }
 +
  p.res{
 +
    font-size:200%;
 +
  }
 +
  p.pic{
 +
    text-align:center;
 +
    margin-left:0 auto;
 +
  }
 +
  p.caption{
 +
    color:#000000;
 +
    text-align:left;
 +
    font-size: 14.5px;
 +
  }
 +
 
 +
.”caption”{
 +
  margin-left:10%;
 +
  margin-right:10%;
 +
  text-align:left;
 +
  font-size: 14.5px;
 +
  color:#000000;
 +
}
 +
  ul.reference{
 +
    color:#606060;
 +
    list-style:none;
 +
    margin-left:10%;
 +
    margin-right:10%;
 +
    font-family: serif, 'Times New Roman';
 +
  }
 +
#proof_left {
 +
  text-align:center;
 +
  display: table-cell;
 +
  vertical-align: bottom;
 +
    width: 524px;
 +
}
 +
#proof_right {
 +
  text-align:center;
 +
  display: table-cell;
 +
  vertical-align: bottom;
 +
    width: 524px;
 +
}
  
−
<div class="column full_size judges-will-not-evaluate">
+
#jump{
−
<h3>★  ALERT! </h3>
+
    position:fixed;
−
<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
+
    bottom:10%;
−
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
+
    right:7%;
−
</div>
+
    width:9%;
−
<div class="clear"></div>
+
}
 +
#jump img{
 +
    width:100%;
 +
}
  
−
<div class="column full_width">
+
p.picture{
 +
    text-align:center;
 +
    margin:5%;
 +
}
 +
footer{
 +
    background-color:#ffffff;
 +
}
 +
</style>
 +
<body>
 +
  <div id="BACKGROUND">
 +
  <div id="wrapper">
 +
<h1>Basic Part</h1>
  
  
−
<h1>Basic Parts</h1>
+
 
  
−
<p>
 
−
A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
 
−
</p>
 
  
−
<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
+
  <h5>Rev Protein</h5>
−
<br>
+
  <p>For this year’s iGEM competition, we have chosen to submit Rev protein for the award of <a href="http://parts.igem.org/Part:BBa_K2403000">the Best Basic part (BBa_K2403000)</a>. In eukaryotic cells mature mRNA is exported by Tap/p15 (Mex67p/ Mtr2p in yeast) from the nucleus into the cytoplasm for translation into protein. However, in synthetic biology, other types of RNA with complex structures are frequently designed and used. If RNA with complex structure is expressed in a eukaryotic cell, Tap/p15 cannot export it effectively, and the RNA will remain in the nucleus. To solve this problem, we developed parts for the HIV Rev protein and associated cis-acting RRE (rev response element) for nuclear export of artificial RNA. iGEMers can export their RNA sequences containing an RRE to the cytoplasm if they express Rev protein simultaneously. </p>
−
<h3>Best Basic Part Special Prize</h3>
+
  
−
<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are *many* opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
+
<p class="pic"><img src="https://static.igem.org/mediawiki/2017/d/de/Kyoto_fig6b.jpeg" width="60%"></p>
 +
 
 +
  <h5>How Rev works</h5>
  
−
<br><br>
+
  <p>Rev recruits CRM1, a principal nuclear export factor in cells which mediates export of cellular RNAs including snRNA and all 4 ribosomal RNAs to the cytoplasm.</p>
−
<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
+
 
 +
  <h5>Key advantage</h5>
 +
 
 +
  <p>Rev protein makes it possible to export complex RNA structures from the nucleus to the cytoplasm, which otherwise cannot be achieved effectively.
 
<br>
 
<br>
−
 
+
<br>
−
 
+
</p>
−
 
+
 
−
<div class="highlight">
+
  </div>
−
<h4>Note</h4>
+
  </div>
−
<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
+
</body>
−
 
+
−
 
+
−
</div>
+
−
 
+
−
 
+
 
</html>
 
</html>

Latest revision as of 22:21, 1 November 2017

Basic Part

Rev Protein

For this year’s iGEM competition, we have chosen to submit Rev protein for the award of the Best Basic part (BBa_K2403000). In eukaryotic cells mature mRNA is exported by Tap/p15 (Mex67p/ Mtr2p in yeast) from the nucleus into the cytoplasm for translation into protein. However, in synthetic biology, other types of RNA with complex structures are frequently designed and used. If RNA with complex structure is expressed in a eukaryotic cell, Tap/p15 cannot export it effectively, and the RNA will remain in the nucleus. To solve this problem, we developed parts for the HIV Rev protein and associated cis-acting RRE (rev response element) for nuclear export of artificial RNA. iGEMers can export their RNA sequences containing an RRE to the cytoplasm if they express Rev protein simultaneously.

How Rev works

Rev recruits CRM1, a principal nuclear export factor in cells which mediates export of cellular RNAs including snRNA and all 4 ribosomal RNAs to the cytoplasm.

Key advantage

Rev protein makes it possible to export complex RNA structures from the nucleus to the cytoplasm, which otherwise cannot be achieved effectively.