Difference between revisions of "Team:Kyoto/Composite Part"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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    <h1>Composite part</h1>
  
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<h1>Composite Parts</h1>
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  <p>In this section, we nominate our Best Composite Part: T7 promoter-RRE ( <a href="http://parts.igem.org/Part:BBa_K2403002"> BBa_K2403002</a>). Expression of RNA with an RRE (Rev Response Element), provides a way to achieve nuclear export of any RNA, especially those with complex structure. </p>
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  <h5>Best Composite Part</h5>
  
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We combined our RRE part (<a href="http://parts.igem.org/Part:BBa_K2403001">BBa_K2403001</a>) along with a T7 promoter (<a href="http://parts.igem.org/Part:BBa_K1614000">BBa_K1614000</a>) for RNA expression in the high copy BioBrick assembly plasmid pSB1C3.</p>
  
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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  <p> iGEMers can simply digest our composite part <a href="http://parts.igem.org/Part:BBa_K2403002">BBa_K2403002</a> with the restriction enzymes <i>XbaI</i> and <i>SpeI</i>, and ligate their custom sequence which will be expressed as an RNA that includes an RRE. Along with expression of Rev protein, they can easily export their RNA from the nucleus.</p>
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  <p>This part makes it possible for complex RNA to be transported to the cytoplasm by CRM1.<a href="https://2017.igem.org/Team:Kyoto/Results">Please see our result for more detailed information.</a>
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
 
  
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<h3>Best Composite Part Special Prize</h3>
 
  
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<p>New BioBrick devices can be made by combining existing BioBrick Parts. For example, Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
 
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
 
  
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Latest revision as of 22:22, 1 November 2017

Composite part

In this section, we nominate our Best Composite Part: T7 promoter-RRE ( BBa_K2403002). Expression of RNA with an RRE (Rev Response Element), provides a way to achieve nuclear export of any RNA, especially those with complex structure.

Best Composite Part

Construction We combined our RRE part (BBa_K2403001) along with a T7 promoter (BBa_K1614000) for RNA expression in the high copy BioBrick assembly plasmid pSB1C3.

写真

iGEMers can simply digest our composite part BBa_K2403002 with the restriction enzymes XbaI and SpeI, and ligate their custom sequence which will be expressed as an RNA that includes an RRE. Along with expression of Rev protein, they can easily export their RNA from the nucleus.

This part makes it possible for complex RNA to be transported to the cytoplasm by CRM1.Please see our result for more detailed information.