Difference between revisions of "Team:IIT Delhi/recipe"

 
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<div class = "navbar ">
 
<div class = "navbar ">
 
 
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                                                         <button class="dropbtn1"><a href="https://2017.igem.org/Team:IIT_Delhi">iGEM IIT Delhi</a></button>
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                                                         <button class="dropbtn2"><a href="https://2017.igem.org/Team:IIT_Delhi">iGEM IIT Delhi</a></button>
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<div class = "right_menu">
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        <div class = "right_menu">
 
   <div class="dropdown">
 
   <div class="dropdown">
 
     <button class="dropbtn">Project  
 
     <button class="dropbtn">Project  
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     </button>
 
     </button>
 
     <div class="dropdown-content">
 
     <div class="dropdown-content">
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       <a href="/Team:IIT_Delhi/Description">Description</a>
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       <a href="/Team:IIT_Delhi/Description">Overview</a>
−
       <a href="/Team:IIT_Delhi/Results">Results</a>
+
       <a href="/Team:IIT_Delhi/Design">Squarewave Generator</a>
  
−
       <a href="/Team:IIT_Delhi/Interlab">Interlab</a>
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       <a href="/Team:IIT_Delhi/InterLab">Interlab</a>
 
     </div>
 
     </div>
 
   </div>  
 
   </div>  
 
                                                         <div class="dropdown">
 
                                                         <div class="dropdown">
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    <button class="dropbtn">Results
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    <i class="fa fa-caret-down"></i>
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    </button>
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    <div class="dropdown-content">
 +
      <a href="/Team:IIT_Delhi/Circuit_Design">Circuit design and construction</a>
 +
      <a href="/Team:IIT_Delhi/Microfluidics">Microfluidics and Fluorescence</a>
 +
      <a href="/Team:IIT_Delhi/Photobleaching">Photobleaching</a>
 +
      <a href="/Team:IIT_Delhi/Promoter">Promoter strength</a>
 +
                                                      <a href="/Team:IIT_Delhi/Oscillations">Oscillations</a>
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 +
    </div>
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  </div>
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 +
                                                          <div class="dropdown">
 
     <button class="dropbtn">Parts  
 
     <button class="dropbtn">Parts  
 
     <i class="fa fa-caret-down"></i>
 
     <i class="fa fa-caret-down"></i>
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       <a href="/Team:IIT_Delhi/Improved_Part">Improved Parts</a>
 
       <a href="/Team:IIT_Delhi/Improved_Part">Improved Parts</a>
 
       <a href="/Team:IIT_Delhi/Part_Collection">Part Collection</a>
 
       <a href="/Team:IIT_Delhi/Part_Collection">Part Collection</a>
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     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="dropdown">
 
   <div class="dropdown">
 
     <button class="dropbtn">Modeling
 
     <button class="dropbtn">Modeling
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     <div class="dropdown-content-big">
 
     <div class="dropdown-content-big">
 
       <a href="/Team:IIT_Delhi/Model">Overview</a>
 
       <a href="/Team:IIT_Delhi/Model">Overview</a>
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       <a href="/Team:IIT_Delhi/Database">Simulation Database</a>
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       <a href="/Team:IIT_Delhi/Write_Model">Writing a Model</a>
 +
                                                        <a href="/Team:IIT_Delhi/Deterministic_Model">Deterministic Model </a>
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                                                        <a href="/Team:IIT_Delhi/Stochastic_Model">Stochastic Model</a>
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                                                        <a href="/Team:IIT_Delhi/Bifurcation">Bifurcation and Squareness</a>
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                                                        <a href="/Team:IIT_Delhi/Resources">Resource sharing</a>
 
     </div>
 
     </div>
 
     </div>
 
     </div>
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       <a href="/Team:IIT_Delhi/Engagement">Public Engagement</a>
 
       <a href="/Team:IIT_Delhi/Engagement">Public Engagement</a>
 
       <a href="/Team:IIT_Delhi/Collaborations">Collaborations</a>
 
       <a href="/Team:IIT_Delhi/Collaborations">Collaborations</a>
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                                                        <a href="/Team:IIT_Delhi/Safety">Safety</a>
 
     </div>
 
     </div>
 
   </div>  
 
   </div>  
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 +
                                                        <div class="dropdown">
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    <button class="dropbtn">Collaborations
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    <i class="fa fa-caret-down"></i>
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    </button>
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    <div class="dropdown-content">
 +
                                                        <a href="/Team:IIT_Delhi/Collaborations">Overview</a>
 +
      <a href="/Team:IIT_Delhi/GMM_legislation">GMM Legislation</a>
 +
      <a href="/Team:IIT_Delhi/berlin">iGEM Berlin</a>
 +
      <a href="/Team:IIT_Delhi/mohali">Mentoring IISER Mohali</a>
 +
      <a href="/Team:IIT_Delhi/glasgow">iGEM Glasgow</a>
 +
             
 +
    </div>
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  </div> 
 
   <div class="dropdown">
 
   <div class="dropdown">
 
     <button class="dropbtn">Notebook  
 
     <button class="dropbtn">Notebook  
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             <h2 class="h2font">RECIPES</h2>
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             <h2 class="h2font">Recipes</h2>
  
 
             <p> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
             <p> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
  </p>
 
  </p>
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<h2 id="pfont">Accurate Experimenting for Accurate Results !!</h2>
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<h2 id="pfont">A pinch of salt makes all the difference !!</h2>
 
           </header>
 
           </header>
 
           <button class="accordion back1" style="font-weight: bold;">Preparation of TAE buffer</button>
 
           <button class="accordion back1" style="font-weight: bold;">Preparation of TAE buffer</button>
 
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           <div align="left" style="font-size:84%;">  
 
           <div align="left" style="font-size:84%;">  
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            20x TAE buffer
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            &nbsp;&nbsp;&nbsp;&nbsp; 20x TAE buffer
 
           <ul>
 
           <ul>
 
           <li>80mL of 1M Tris solution
 
           <li>80mL of 1M Tris solution
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           <li>0.7264g of EDTANa2 salt
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           <li>0.7264g of EDTANa2+ salt
 
           <li>2.284 mL glacial acetic acid
 
           <li>2.284 mL glacial acetic acid
 
           <li>distilled H2O 100mL
 
           <li>distilled H2O 100mL
 
           </ul>
 
           </ul>
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<br>
 
</div>
 
</div>
 
</div>
 
</div>
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           <div class="panel">
 
           <div align="left" style="font-size:84%;">  
 
           <div align="left" style="font-size:84%;">  
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           <ul>For the preparation of ethidium bromide adds 1 g of ethidium bromide to 100 ml of H2O.
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           <ul>
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Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved. Wrap
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<li>For the preparation of ethidium bromide adds 1 g of ethidium bromide to 100 ml of H2O.
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the container in aluminum foil or transfer the 10 mg/ml solution to a dark bottle and
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<li>Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved.
 +
<li> Wrap the container in aluminum foil or transfer the 10 mg/ml solution to a dark bottle and
 
store at room temperature. </ul>
 
store at room temperature. </ul>
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<br>
 
           </div>
 
           </div>
 
           </div>
 
           </div>
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           <li>10 mM EDTA (pH 8.0).
 
           <li>10 mM EDTA (pH 8.0).
 
           </ul>
 
           </ul>
−
           Prepare Solution I from standard stocks in batches of approx. 100 ml, sterilize by
+
           <ul>Prepare Solution I from standard stocks in batches of approx. 100 ml, sterilize by
−
autoclaving and store at 4°C.
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autoclaving and store at 4°C.</ul>
 +
<br>
 
           </div>
 
           </div>
 
           </div>
 
           </div>
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           <div align="left" style="font-size:84%;">  
 
           <div align="left" style="font-size:84%;">  
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           <ul>0.2 N NaOH (freshly diluted from a 10 N stock).(100ulsds + 700ul mq + 200ul naoh)
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           <ul>
 +
<li>0.2 N NaOH (freshly diluted from a 10 N stock).
 +
<li>(100ul SDS + 700ul mq + 200ul NaOH)
 
1% (w/v) SDS.</ul>
 
1% (w/v) SDS.</ul>
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<br>
 
           </div>
 
           </div>
 
</div>
 
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           <ul>The resulting solution is 3 M with respect to potassium and 5 M with respect to acetate.
 
           <ul>The resulting solution is 3 M with respect to potassium and 5 M with respect to acetate.
 
Store the solution at 4°C and transfer it to an ice bucket just before use.</ul>
 
Store the solution at 4°C and transfer it to an ice bucket just before use.</ul>
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<br>
 
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Latest revision as of 22:22, 1 November 2017

iGEM IIT Delhi

Overview Squarewave Generator Interlab
Circuit design and construction Microfluidics and Fluorescence Photobleaching Promoter strength Oscillations
Basic Parts Composite Parts Improved Parts Part Collection
Overview Writing a Model Deterministic Model Stochastic Model Bifurcation and Squareness Resource sharing
Human Practices Integrated Practices Public Engagement Collaborations Safety
Overview GMM Legislation iGEM Berlin Mentoring IISER Mohali iGEM Glasgow
Lab Records Protocols Recipes Medal Criteria
The Team Attributions

Recipes

                                                                                                                                                                                                                 

A pinch of salt makes all the difference !!

     20x TAE buffer
  • 80mL of 1M Tris solution
  • 0.7264g of EDTANa2+ salt
  • 2.284 mL glacial acetic acid
  • distilled H2O 100mL

  • For the preparation of ethidium bromide adds 1 g of ethidium bromide to 100 ml of H2O.
  • Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved.
  • Wrap the container in aluminum foil or transfer the 10 mg/ml solution to a dark bottle and store at room temperature.

  • 50 mM glucose.
  • 25 mM Tris-Cl (pH 8.0).
  • 10 mM EDTA (pH 8.0).
    Prepare Solution I from standard stocks in batches of approx. 100 ml, sterilize by autoclaving and store at 4°C.

  • 0.2 N NaOH (freshly diluted from a 10 N stock).
  • (100ul SDS + 700ul mq + 200ul NaOH) 1% (w/v) SDS.

  • 5 M potassium acetate, 60.0 ml
  • Glacial acetic acid, 11.5 ml
  • H2O, 28.5 ml
    The resulting solution is 3 M with respect to potassium and 5 M with respect to acetate. Store the solution at 4°C and transfer it to an ice bucket just before use.


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Contact Us Address

E-mail: iitd.igem@gmail.com
Undergraduate Laboratory
Department of Biotechnology and Biochemical Engineering, IIT Delhi