Difference between revisions of "Team:IIT Delhi/recipe"
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background-position: center; | background-position: center; | ||
background-repeat: no-repeat; | background-repeat: no-repeat; | ||
| − | background-size: | + | background-size: cover ; |
| − | + | padding-bottom: 150px; | |
color: #ffffff; | color: #ffffff; | ||
padding: 27.5em 0em 1em; | padding: 27.5em 0em 1em; | ||
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z-index: 101; | z-index: 101; | ||
} | } | ||
| + | |||
| + | .dropbtn2 { | ||
| + | font-size: 16px; | ||
| + | border: none; | ||
| + | outline: none; | ||
| + | color: white; | ||
| + | padding: 15px 16px; | ||
| + | background-color: #000; | ||
| + | background-size: 100% 100%; | ||
| + | z-index: 101; | ||
| + | } | ||
| + | |||
.navbar a:hover, .dropdown:hover .dropbtn { | .navbar a:hover, .dropdown:hover .dropbtn { | ||
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<div class = "navbar "> | <div class = "navbar "> | ||
| − | <button class=" | + | <button class="dropbtn2"><a href="https://2017.igem.org/Team:IIT_Delhi">iGEM IIT Delhi</a></button> |
| − | + | <div class = "right_menu"> | |
<div class="dropdown"> | <div class="dropdown"> | ||
<button class="dropbtn">Project | <button class="dropbtn">Project | ||
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</button> | </button> | ||
<div class="dropdown-content"> | <div class="dropdown-content"> | ||
| − | <a href="/Team:IIT_Delhi/Description"> | + | <a href="/Team:IIT_Delhi/Description">Overview</a> |
| − | <a href="/Team:IIT_Delhi/ | + | <a href="/Team:IIT_Delhi/Design">Squarewave Generator</a> |
| − | <a href="/Team:IIT_Delhi/ | + | <a href="/Team:IIT_Delhi/InterLab">Interlab</a> |
</div> | </div> | ||
</div> | </div> | ||
<div class="dropdown"> | <div class="dropdown"> | ||
| + | <button class="dropbtn">Results | ||
| + | <i class="fa fa-caret-down"></i> | ||
| + | </button> | ||
| + | <div class="dropdown-content"> | ||
| + | <a href="/Team:IIT_Delhi/Circuit_Design">Circuit design and construction</a> | ||
| + | <a href="/Team:IIT_Delhi/Microfluidics">Microfluidics and Fluorescence</a> | ||
| + | <a href="/Team:IIT_Delhi/Photobleaching">Photobleaching</a> | ||
| + | <a href="/Team:IIT_Delhi/Promoter">Promoter strength</a> | ||
| + | <a href="/Team:IIT_Delhi/Oscillations">Oscillations</a> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
| + | |||
| + | <div class="dropdown"> | ||
<button class="dropbtn">Parts | <button class="dropbtn">Parts | ||
<i class="fa fa-caret-down"></i> | <i class="fa fa-caret-down"></i> | ||
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<a href="/Team:IIT_Delhi/Improved_Part">Improved Parts</a> | <a href="/Team:IIT_Delhi/Improved_Part">Improved Parts</a> | ||
<a href="/Team:IIT_Delhi/Part_Collection">Part Collection</a> | <a href="/Team:IIT_Delhi/Part_Collection">Part Collection</a> | ||
| + | |||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
<div class="dropdown"> | <div class="dropdown"> | ||
<button class="dropbtn">Modeling | <button class="dropbtn">Modeling | ||
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<div class="dropdown-content-big"> | <div class="dropdown-content-big"> | ||
<a href="/Team:IIT_Delhi/Model">Overview</a> | <a href="/Team:IIT_Delhi/Model">Overview</a> | ||
| − | <a href="/Team:IIT_Delhi/ | + | <a href="/Team:IIT_Delhi/Write_Model">Writing a Model</a> |
| + | <a href="/Team:IIT_Delhi/Deterministic_Model">Deterministic Model </a> | ||
| + | <a href="/Team:IIT_Delhi/Stochastic_Model">Stochastic Model</a> | ||
| + | <a href="/Team:IIT_Delhi/Bifurcation">Bifurcation and Squareness</a> | ||
| + | <a href="/Team:IIT_Delhi/Resources">Resource sharing</a> | ||
</div> | </div> | ||
</div> | </div> | ||
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<a href="/Team:IIT_Delhi/Engagement">Public Engagement</a> | <a href="/Team:IIT_Delhi/Engagement">Public Engagement</a> | ||
<a href="/Team:IIT_Delhi/Collaborations">Collaborations</a> | <a href="/Team:IIT_Delhi/Collaborations">Collaborations</a> | ||
| + | <a href="/Team:IIT_Delhi/Safety">Safety</a> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | |||
| + | <div class="dropdown"> | ||
| + | <button class="dropbtn">Collaborations | ||
| + | <i class="fa fa-caret-down"></i> | ||
| + | </button> | ||
| + | <div class="dropdown-content"> | ||
| + | <a href="/Team:IIT_Delhi/Collaborations">Overview</a> | ||
| + | <a href="/Team:IIT_Delhi/GMM_legislation">GMM Legislation</a> | ||
| + | <a href="/Team:IIT_Delhi/berlin">iGEM Berlin</a> | ||
| + | <a href="/Team:IIT_Delhi/mohali">Mentoring IISER Mohali</a> | ||
| + | <a href="/Team:IIT_Delhi/glasgow">iGEM Glasgow</a> | ||
| + | |||
| + | </div> | ||
| + | </div> | ||
<div class="dropdown"> | <div class="dropdown"> | ||
<button class="dropbtn">Notebook | <button class="dropbtn">Notebook | ||
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| − | <h2 class="h2font"> | + | <h2 class="h2font">Recipes</h2> |
<p> | <p> | ||
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| | ||
</p> | </p> | ||
| − | <h2 id="pfont"> | + | <h2 id="pfont">A pinch of salt makes all the difference !!</h2> |
</header> | </header> | ||
<button class="accordion back1" style="font-weight: bold;">Preparation of TAE buffer</button> | <button class="accordion back1" style="font-weight: bold;">Preparation of TAE buffer</button> | ||
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<ul> | <ul> | ||
<li>80mL of 1M Tris solution | <li>80mL of 1M Tris solution | ||
| − | <li>0.7264g of EDTANa2 salt | + | <li>0.7264g of EDTANa2+ salt |
<li>2.284 mL glacial acetic acid | <li>2.284 mL glacial acetic acid | ||
<li>distilled H2O 100mL | <li>distilled H2O 100mL | ||
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<div class="panel"> | <div class="panel"> | ||
<div align="left" style="font-size:84%;"> | <div align="left" style="font-size:84%;"> | ||
| − | <ul>0.2 N NaOH (freshly diluted from a 10 N stock).(100ul SDS + 700ul mq + 200ul NaOH) | + | <ul> |
| + | <li>0.2 N NaOH (freshly diluted from a 10 N stock). | ||
| + | <li>(100ul SDS + 700ul mq + 200ul NaOH) | ||
1% (w/v) SDS.</ul> | 1% (w/v) SDS.</ul> | ||
<br> | <br> | ||
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<br> | <br> | ||
</div> | </div> | ||
| − | + | ||
</div> | </div> | ||
| − | + | <br> | |
<script> | <script> | ||
var acc = document.getElementsByClassName("accordion"); | var acc = document.getElementsByClassName("accordion"); | ||
Latest revision as of 22:22, 1 November 2017
Recipes
A pinch of salt makes all the difference !!
20x TAE buffer
- 80mL of 1M Tris solution
- 0.7264g of EDTANa2+ salt
- 2.284 mL glacial acetic acid
- distilled H2O 100mL
- For the preparation of ethidium bromide adds 1 g of ethidium bromide to 100 ml of H2O.
- Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved.
- Wrap the container in aluminum foil or transfer the 10 mg/ml solution to a dark bottle and store at room temperature.
- 50 mM glucose.
- 25 mM Tris-Cl (pH 8.0).
- 10 mM EDTA (pH 8.0).
- Prepare Solution I from standard stocks in batches of approx. 100 ml, sterilize by
autoclaving and store at 4°C.
- 0.2 N NaOH (freshly diluted from a 10 N stock).
- (100ul SDS + 700ul mq + 200ul NaOH) 1% (w/v) SDS.
- 5 M potassium acetate, 60.0 ml
- Glacial acetic acid, 11.5 ml
- H2O, 28.5 ml
- The resulting solution is 3 M with respect to potassium and 5 M with respect to acetate.
Store the solution at 4°C and transfer it to an ice bucket just before use.
