Difference between revisions of "Team:Sheffield/Demonstrate"
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<p align="center">The slight unexpected variations are due to noise, however we are able to remove this by smoothing the data. To see more information about this please look at "Smoothing" under the software section. An example of a graph that has been smoothed is shown below:</p> | <p align="center">The slight unexpected variations are due to noise, however we are able to remove this by smoothing the data. To see more information about this please look at "Smoothing" under the software section. An example of a graph that has been smoothed is shown below:</p> | ||
| − | <p align="center"> | + | <img style="width:750px;" src="https://static.igem.org/mediawiki/2017/7/76/T--Sheffield--smoothedCurve.png" class="img-responsive center" style="display:inline"></p> |
| + | <h4 align="center">Figure 2: The Figure above shows a bacterial growth after it has been smoothed to reduce noise. </h4> | ||
| + | </br> | ||
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| + | <p align="center">To run the experiment above we used different dilutions of bacteria. To do this we first grew a culture of E. coli overnight. We then took 1ml of this culture and diluted it in 9ml of LB media. This dilution went into all of the wells in the first row of a 96 well-plate. We then took 1ml from the first dilution and diluted it again with another 9ml of LB media, and this dilution went into all the wells in the 2nd row of the plate. This process was repeated until the entire plate was filled. We did this so that we would have different concentrations of bacteria across the plate.</p> | ||
| + | |||
| + | <p align="center">We repeated this process with 2 plates, using the same dilutions in each of the rows of the plate. We than ran a calibration experiment between our device, which contained 1 plate, and a Victor plate reader with the 2nd plate. As the 1st and 2nd plate were prepared the same, with the same bacteria culture the growth of the bacteria in the two plate would be the same, hence we are able to run a calibration experiment between the two devices with the two plates.</p> | ||
</div> | </div> | ||
Revision as of 22:23, 1 November 2017
Demonstrate
To demonstrate that our device works, we performed a bacterial growth experiment and used our device to detect the changes in turbidity that occurred due to the bacterial growth. As the bacteria grow and the solution becomes more turbid, more light is attenuated as it passes through the sample in the well. The transmittance of light that reaches the photo-diode is therefore decreased and this decrease of light causes the photo-diode to produce less current across it, and therefore the potential across the photo-diode also drops. This change in potential is measured using a potential divider between the photo-diode and a resistor. As the voltage across the photo-diode decreases the voltage across the resistor increases, meaning if the bacteria are growing we will see the voltage across the resistor increasing. This is shown in the graph below:
Figure 1: The Figure above shows a bacterial growth curve that was measured with our device. As bacteria grow and block the light the voltage across the photo-diode decreases and therefore the voltage across the resistor increases. The increase of the voltage across the resistor as the bacteria grow is what we can see above.
The slight unexpected variations are due to noise, however we are able to remove this by smoothing the data. To see more information about this please look at "Smoothing" under the software section. An example of a graph that has been smoothed is shown below:
Figure 2: The Figure above shows a bacterial growth after it has been smoothed to reduce noise.
To run the experiment above we used different dilutions of bacteria. To do this we first grew a culture of E. coli overnight. We then took 1ml of this culture and diluted it in 9ml of LB media. This dilution went into all of the wells in the first row of a 96 well-plate. We then took 1ml from the first dilution and diluted it again with another 9ml of LB media, and this dilution went into all the wells in the 2nd row of the plate. This process was repeated until the entire plate was filled. We did this so that we would have different concentrations of bacteria across the plate.
We repeated this process with 2 plates, using the same dilutions in each of the rows of the plate. We than ran a calibration experiment between our device, which contained 1 plate, and a Victor plate reader with the 2nd plate. As the 1st and 2nd plate were prepared the same, with the same bacteria culture the growth of the bacteria in the two plate would be the same, hence we are able to run a calibration experiment between the two devices with the two plates.
What should we do for our demonstration?
Standard teams
If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field. Please note biological materials must not be taken out of the lab.
Special track teams
Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree. Please note biological materials must not be taken out of the lab.
